Abstract
Chitin is one of the most abundant organic compounds on earth. It is present in both animals and fungi, and characteristically it is absent in plants. In nature, the most abundant crystalline form of the polymer is α-chitin, which occurs in the form of microfibrils. Microfibrils have been observed by electron microscopy of fungal cell walls by use of different techniques: shadow-casting, heavy-metal replicas, negative staining, and oblique and tangential sections. In the cell walls of fungi, chitin microfibrils constitute the skeleton responsible for their rigidity (for a review, see Ruiz-Herrera, 1978).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Aoyagi, T., Umezawa, H., 1975, Structures and activities of protease inhibitors of microbial origin, in: Proteases and Biological Control ( E. Reich, D. B. Rifkin, E. Shaw, eds.), pp. 429–454, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Arroyo-Begovich, A., Ruiz-Herrera, J., 1979, Proteolytic activation and inactivation of chitin synthase from Neurospora crassa. J. Gen. Microbiol. 113: 339.
Bartnicki-García, S., Lippman, E., 1972, Inhibition of Mucor rouxii by polyoxin: Effects on chitin synthetase and morphological development. J. Gen. Microbiol. 71: 301.
Bartnicki-García, S., Bracker, C. E., Ruiz-Herrera, J., 1977, Reassembly of solubilized chitin synthetase into functional structures resembling chitosomes, Abstracts of the Second International Mycological Congress, Tampa, Florida, p. 41.
Bartnicki-García, S., Bracker, C. E., Ruiz-Herrera, J., 1978, Isolation of chitosomes from taxonomically diverse fungi and synthesis of chitin microfibrils in vitro. Exp. My col. 2: 173.
Bartnicki-García, S., Lippman, E.,Heick, J., 1979, Evidence for a polypeptide acceptor in fungal chitin biosynthesis, Abstracts of the Annual Meeting of the American Society for Microbiology, Los Angeles, p. 106.
Bartnicki-García, S., Bracker, C. A., Ruiz-Herrera, J., 1980, Isolation of chitosomes from osmotically fragile cells of Mucer rouxii and Neurospora crassa, Abstracts of the Annual Meeting of the American Society of Microbiology, Los Angeles, p. 98.
Benítez, T., Villa, T. G., García, Acha, I., 1976, Effects of polyoxin D on germination, morphological development and biosynthesis of cell wall of Trichoderma viride. Arch. Microbiol in vitro. 108: 183.
Betz, H., Hinze, H., Holzer, H., 1974, Isolation and properties of two inhibitors of proteinase B from yeast. J. Biol. Chem. 249: 4515.
Blackwell, J., Minke, R., Gardner, K. H., 1978, Determination of the structures of α- and β-chitins by X-ray diffraction, in: Proceedings of the First International Conference on Chitin/Chitosan (R. A. A. Muzzarelli, E. R. Pariser, eds.), pp. 108–123, MIT Sea Grant Report MITSG 78-7, Boston.
Bowers, B., Levin, G., Cabib, E., 1974, Effect of polyoxin D on chitin synthesis and septum formation in Saccharomyces cerevisiae. J. Bacteriol. 119: 564.
Bracker, C. E., Ruiz-Herrera, J., Bartnicki-García, S., 1976, Structure and transformation and chitin synthetase particles (chitosomes) during microfibril synthesis in vitro. Proc. Natl. Acad. Sci. U.S.A. 73: 4570.
Braun, P. C., Calderone, R. A., 1978, Chitin synthesis in Candida albicans: Comparison of yeast and hyphal forms. J. Bacteriol. 135: 1472.
Braun, P. C., Calderone, R. A., 1979, Regulation and solubilization of Candida albicans chitin synthetase. J. Bacteriol. 140: 666.
Cabib, E., Farkas, V., 1971, Control of morphogenesis: An enzymatic mechanism for the initiation of septum formation in yeast. Proc. Natl. Acad. Sci. U.S.A. 68: 2052.
Cabib, E., Keller, F. A., 1971, Chitin and yeast budding: Allosteric inhibition of chitin synthetase by a heat-stable protein from yeast. J. Biol. Chem. 246: 167.
Cabib, E., Ulane, R., 1973, Yeast chitin synthetase: Separation of the zymogen from its activating factor and recovery of the latter in the vacuole fraction. J. Biol. Chem. 248: 1451.
Calvo, C., 1981, Mechanisms de sinteus de quitosana en Mucer rouxii. M. Sc. Thesis., Centro de Investigasion y Estudios Avanjados, Instituto Politicuico Nacinal, Mexico.
Camargo, P. E., Dietrich, C. P., Sonneborn, D., Strominger, J. L., 1967, Biosynthesis of chitin in spores and growing cells of Blastocladiella emersonii. J. Biol. Chem. 242: 3121.
Dahn, U., Hagenmaier, H., Hohne, H., Konig, W. A., Wolf, G., Zahner, H., 1976, Stoffwechselproduckte von Mikroorganismen. 154. Mitteilung. Nikkomycin, ein neuer Hemmstoff der Chitinsynthese bei Pilzen. Arch. Microbiol. 107: 143.
De Rousset-Hall, A., Gooday, G. W., 1975, A kinetic study of solubilized chitin synthetase preparations from Coprinus cinereus. J. Gen. Microbiol. 89: 146.
Díaz, S. V., Ruiz-Herrera, J., 1978, Purificación de una proteasa que inactiva la quitina sintetasa de Mucor rouxii, Resúmenes, Reunion Bianual de Microbiologia, Toluca, Mexico, p. B-11.
Durán, A., Cabib, E., 1978, Solubilization and partial purification of yeast chitin synthetase: Confirmation of the zymogenic nature of the enzyme. J. Biol. Chem. 253: 4419.
Endo, A., Misato, T., 1969, Polyoxin D, a competitive inhibitor of UDP-N-acetyl-glucosamine: Chitin N-acetyl glucosaminyl transferase in Neurospora crassa. Biochem. Biophys Res. Commun 37: 718.
Endo, A., Kakiki, K., Misato, T., 1970, Mechanism of action of the antifungal agent polyoxin D. J. Bacteriol. 104: 189.
Feingold, D. S., Neufeld, E. F.,Hassid, W. Z., 1958, Synthesis of α β1,3 linked glucan by extracts of Phaseolus aureus seedlings. J. Biol. Chem. 133: 783.
Ferguson, A. R., Katsunuma, T., Betz, H., Holzen, H., 1973, Purification and properties of an inhibitor of the tryptophan-synthase inactivating enzymes in yeast. Eur. J. Biochem. 32: 444.
Glaser, L., Brown, D. H., 1957, The synthesis of chitin in cell-free extracts of Neurospora crassa. J. Biol. Chem. 228: 729.
Gooday, G. W., 1977, Biosynthesis of the fungal wall: Mechanisms and implications. J. Gen. Microbiol in vitro. 99: 1.
Gooday, G. W., De Rousset-Hall, A., 1975, Properties of chitin synthetase from Coprinus cinereus. J. Gen. Microbiol. 89: 137.
Hassilik, A., 1974, Inactivation of chitin synthetase in Saccharomyces cerevisiae. Arch. Microbiol. 101: 295.
Hernandez, J. Lopez-Romero, E., Cerbon, J., Ruiz-Herrera, J. Lipid analysis of chitosomes, chitin synthesizing microvesicles, Experimental Mycol. in press.
Holzer, H., Saheki, T., 1976, Mechanism and regulation of proteolytic processes in yeast. Tokai J. Exp. Clin. Med. 1: 115.
Hori, M., Kakiki, K., Misato, T., 1974a, Further study on the relation of polyoxin structure of chitin synthetase inhibition. Agric. Biol. Chem. 38: 691.
Hori, M., Kakiki, K., Misato, T., 1974b, Interaction between polyoxin and active center of chitin synthetase. Agric. Biol. Chem. 38: 699.
Isono, K., Asahi, K., Suzuki, S., 1969, Studies on polyoxins, antifungal antibiotics. XII. The structure of polyoxins. J. Am. Chem. Soc. 91: 7490.
Jan, Y. N., 1974, Properties and cellular localization of chitin synthetase in Phycornyces blakesleeanus. J. Biol. Chem. 249: 1973.
Jaworski, E. G., Wang, L., Marco, G., 1963, Synthesis of chitin in cell-free extracts of Prodenia eridania. Nature (London) 198: 790.
Jaworski, E. G., Wang, L. C., Carpenter, W. D., 1965, Biosynthesis of chitin in cell-free extracts of Venturia inaequalis. Phytopathology 55: 1309.
Katz, D., Rosenberger, R. F., 1970, A mutation in Aspergillus nidulans producing hyphal walls which lack chitin. Biochim. Biophys. Acta 208: 452.
Keller, F. A., Cabib, E., 1971, Chitin and yeast budding: Properties of chitin synthetase from Saccharomyces carlsbergensis. J. Biol. Chem. 246: 160.
Leloir, L. F., 1972, Biosynthesis of polysaccharides seen from Buenos Aires, in: Biochemistry of the Glycosidic Linkage ( R. Piras, H. G., Ponds, eds.), pp. 1–17, Academic Press, New York.
Lenney, J. F., 1975, Three yeast proteins that specifically inhibit yeast proteases A, B, C. J. Bacteriol. 122: 1265.
Lenney, J. F., Dalbec, J. M., 1967, Purification and properties of two proteinases from Saccharomyces cerevisiae. Arch. Biochem. Biophys. 120: 42.
Lenney, J. F., Dalbec, J. M., 1969, Yeast proteinase B: Identification of the inactive form as an enzyme-inhibitor complex. Arch. Biochem. Biophys. 129: 407.
Lenney, J. F., Matile, P., Weimken, A., Schellenberg, M., Meyer, J., 1974, Activities and cellular localization of yeast proteases and their inhibitors. Biochem. Biophys. Res. Commun. 60: 1378.
Lopez-Romero, E., Ruiz-Herrera, J., 1976, Synthesis of chitin by particulate preparations from Aspergillus flavus, Antonie van Leeuwenhoek. J. Microbiol. Serol. 42: 261.
Lopez-Romero, E., Ruiz-Herrera, J., Bartnicki-Garcia, S., 1978, Purification and properties of an inhibitory protein of chitin synthetase from Mucor rouxii. Biochim. Biophys. Acta 525: 338.
McMurrough, I., Bartnicki-Garcia, S., 1971, Properties of a particulate chitin synthetase from Mucor rouxii, J. Biol. Chem. 246: 4008.
McMurrough, I., Bartnicki-Garcia, S., 1973, Inhibition and activation of chitin synthesis by Mucor rouxii cell extracts. Arch. Biochem. Biophys. 158: 812.
McMurrough, I., Flores-Carreon, A., Bartnicki-Garcia, S., 1971, Pathway of chitin synthesis and cellular localization of chitin synthetase in Mucor rouxii. J. Biol. Chem. 246: 3999.
Mills, G. L.,Cantino, E., 1978, The lipid composition of the Blastocladiella emersonii γ-particle and the function of particle lipid in chitin formation. Exp. Mycol. 2: 99.
Moore, P. M., Peberdy, J. F., 1975, Biosynthesis of chitin by particulate fractions from Cunninghamella elegans. Microbios 12: 29.
Moore, P. M.,and Peberdy, J. F., 1976, A particulate chitin synthetase from Aspergillus flavus Link: The properties, location, levels of activity in mycelium and regenerating protoplast preparations. Can. J. Microbiol. 22: 915.
Myers, R. B., Cantino, C. E., 1974, The Gamma Particle, S. Karger, Basel.
Nanba, H., Kuroda, H., 1974, Studies on fungicides. X. Biosynthesis of β-glucan and chitin-like substance of cell wall from Cochliobolus miyabeanus. Chem. Pharm. Bull. 22: 610.
Ohta, N., Kakiki, K.,Misato, T., 1970, Studies on the mode of action of polyoxin D. Part II. Effect of polyoxin D on the synthesis of fungal cell wall chitin. Agric. Biol. Chem. 34: 1224.
Parodi, A. J., Leloir, L. F., 1979, The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell. Biochim. Biophys. Acta 559: 1.
Peberdy, J. F., Moore, P. M., 1975, Chitin synthetase in Mortierella vinacea: Properties, cellular location and synthesis in growing cultures. J. Gen. Microbiol. 90: 228.
Porter, C. A., Jaworski, E. G., 1966, The synthesis of chitin by particulate preparations of Allomyces macrogynus. Biochemistry 5: 1149.
Ruiz-Herrera, J., 1978, The distribution and quantitative importance of chitin in fungi, in: Proceedings of the First International Conference of Chitin Chitosan (R. A. A. Muzzarelli, E. R. Pariser, eds.), pp. 11–21, MIT Sea Grant Report MITSG 78-7, Boston.
Ruiz-Herrera, J., Bartnicki-Garcia, S., 1974, Synthesis of cell wall microfibrils in vitro by a “soluble” chitin synthetase from Mucorrouxii. Science 186: 357.
Ruiz-Herrera, J., Bartnicki-Garcia, S., 1976, Proteolytic activation and inactivation of chitin synthetase from Mucor rouxii, J. Gen. Microbiol. 97: 241.
Ruiz-Herrera, J., Sing, V. O., Van Der Woude, W. J., Bartnicki-Garcia, S., 1975, Microfibril assemby by granules of chitin synthetase. Proc. Natl. Acad. Sci. U.S.A. 72: 2706.
Ruiz-Herrera, J., Lopez-Romero, E., Bartnicki-Garcia, S., 1977, Properties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii, J. Biol. Chem. 252: 3338.
Ruiz-Herrera, J., Bartnicki-Garcia, S., Brackner, C. E., 1980, Dissociation of chitosomes by digitonin into IGS subuncts with chitin syntetase activity. Biochem. Biophys. Acta. 629: 201.
Ryder, N. S., Peberdy, J. F. 1977, Chitin synthase in Aspergillus nidulans: Properties and proteolytic activation. J. Gen. Microbiol. 99: 69.
Schott, E. H., Holzer, H., 1974, Purification and some properties of tryptophan synthase inactivase II from yeast. Eur. J. Biochem. 42: 61.
Tsai, C. M., Hassid, W. Z., 1971, Solubilization and separation of uridine diphospho-D-glucose: β - glucan and uridine diphospho-D-glucose: glucan glucosyl transferases from coleoptiles of Avena sativa. Plant Physiol. 47: 740.
Ulane, R. E., Cabib, E., 1974, The activating system of chitin synthetase from Saccharomyces cerevisiae: Purification and properties of an inhibitor of the activating factor. J. Biol. Chem. 249: 3418.
Ulane, R. E., Cabib, E., 1976, The activating system of chitin synthetase from Saccharomyces cerevisiae: Purification and properties of the activating factor. J. Biol. Chem. 251: 3367.
Van Laere, A. J., Carlier, A. R., 1978, Synthesis and proteolytic activation of chitin synthetase in Phycomyces blakesleeanus Burgeff. Arch. Microbiol. 116: 181.
Vermeulen, C. A., Raeven, M. B. J. M., Wessels, J. G. H., 1979, Localization of chitin synthase activity in subcellular fractions of Schizophyllum commune protoplasts. J. Gen. Microbiol. 114: 87.
Wolf, D. H., Ehman, C., 1978, Isolation of yeast mutants lacking proteinase B activity. Febs Lett. 92: 121.
Zubenko, G. S., Mitchell, A. P., Jones, E. W., 1979, Septum formation, cell division, sporulation in mutants of yeast defficient in proteinase B. Proc. Natl. Acad. Sci. U.S.A. 76: 2395.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Ruiz-Herrera, J. (1982). Synthesis of Chitin Microfibrils in Vitro . In: Brown, R.M. (eds) Cellulose and Other Natural Polymer Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1116-4_11
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1116-4_11
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1118-8
Online ISBN: 978-1-4684-1116-4
eBook Packages: Springer Book Archive