Abstract
Glycoproteins have been reported in surface membranes by many laboratories, and the literature has been reviewed (Cook, 1968; Ginsberg and Neufeld, 1969; Korn, 1969; Winzler, 1970; Ginsberg and Kobata, 1971; Kraemer, 1971; Guidotti, 1972; Sharon and Lis, 1973). Several reviews (Spiro, 1970; Marshall, 1972) and a number of books (Jamieson and Greenwalt, 1969, 1971; Aminoff, 1970; Gottschalk, 1972) concerning glycoproteins in general are available, so this chapter is not designed as a comprehensive treatise on the subject. The literature which is quoted is for the purpose of aiding an experimental approach to define surface membrane glycoproteins. Many of the procedures defined here have been described in detail (Ginsberg, 1972).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Agrawal, K. M. L., and Bahl, O. P., 1968, Glycosidases of Phaseolus vulgaris. II. Isolation and general properties, J. Biol. Chem. 243(1):103.
Akiyama, Y., and Osawa, T., 1972, Isolation and characterization of glycoproteins possessing inhibitory activity against various phytohemagglutinins from human group A erythrocytes, Hoppe-Seyler’s Z. Physiol. Chem. 353:323.
Allan, D., Auger, J., and Crumpton, M. J., 1972, Glycoprotein receptors for concanavalin A isolated from pig lymphocyte plasma membrane by affinity chromatography in sodium deoxycholate, Nature New Biol. 236:23.
Aminoff, D., 1970, ed.. Blood and Tissue Antigens, Academic Press, New York.
Aminoff, D., and Furukawa, K., 1970, Enzymes that destroy blood group specificity. I. Purification and properties of α-l-fucosidase from Clostridium perfringens, J. Biol. Chem. 245(7):1659.
Arima, T., and Spiro, R. G., 1972, Studies on the carbohydrate units of thyroglobulin: Structure of the mannose-N-acetylglucosamine unit (unit A) of the human and calf proteins, J. Biol. Chem. 247(6):1836.
Arima, T., Spiro, M. J., and Spiro, R. G., 1972, Studies on the carbohydrate units of thyroglobulin: Evaluation of their microheterogeneity in the human and calf proteins, J. Biol Chem. 247(6):1825.
Ashwell, G., and Morrell, A. G., 1971, Galactose: A cryptic determinant of glycoprotein catabolism, in: Glycoproteins of Blood Cells and Plasma (G. A. Jamieson and T. J. Greenwalt, eds.), pp. 173–189, Lippincott, Philadelphia.
Atkinson, P. H., and Summers, D. P., 1971, Purification and properties of HeLa cell plasma membranes, Biol. Chem. 246(16):5162.
Bahl, O. P., 1970, Glycosidases of Aspergillus niger, J. Biol. Chem. 245(2):299.
Bahl, O. P., and Agrawal, K. M. L., 1968, Glycosidases of Phaseolus vulgaris. I. Isolation and characterization of β-N-acetyglucosaminidase, J. Biol. Chem. 243(1):98.
Barber, A. J., and Jamieson, G. A., 1971, Isolation of glycopeptides from low- and high-density platelet plasma membranes. Biochemistry 10:4711.
Bella, A. M., Jr., and Kim, Y. S., 1970, An improved method of separating glucosaminitol from galactosaminitol and their amino sugars on an amino acid analyzer, J. Chromatog. 51:314.
Bentley, R., 1972, Configurational and conformational aspects of carbohydrate biochemistry, Ann. Rev. Biochem. 41:953.
Björndal, H., and Lundblad, A., 1970, Structure of two urinary oligosaccharides characteristic of blood group O(H)- and B-secretors, Biochim. Biophys. Acta 201:434.
Björndal, H., Lindberg, B., Pilotti, A., and Svensson, S., 1970, Mass spectra of partially methylated alditol acetates. II. Deuterium labelling experiments, Carbohyd. Res. 15:339.
Blumenfeld, O. O., Gallop, P. M., Howe, C., and Lee, L. T., 1970, Erythrocyte membrane proteins: Their study using aqueous pyridine solutions, Biochim. Biophys. Acta 211:109.
Bretscher, M. S., 1971, Major human erythrocyte glycoprotein spans the cell membrane. Nature New Biol. 231:229.
Brown, J. C., 1971, Surface glycoprotein characteristic of the differentiated state of neuroblastoma C-1300 cells, Exptl. Cell Res. 69:440.
Buck, C. A., Glick, M. C., and Warren, L. A., 1970, A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry 9:4567.
Buck, C. A., Glick, M. C., and Warren, L., 1971a, Glycopeptides from the surface of control and virus transformed cells. Science 172:169.
Buck, C. A., Glick, M. C., and Warren, L., 1971b, Effect of growth on the glycopeptides from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry 10:2176.
Burger, M. M., 1968, Isolation of a receptor complex for a tumor specific agglutinin from the neoplastic cell surface. Nature 219:499.
Carlson, D. M., 1968, Structures and immunochemical properties of oligosaccharides isolated from pig submaxillary mucins, J. Biol. Chem. 243(3):616.
Carpenter, F. H., 1967, Treatment of trypsin with TPCK, in: Methods in Enzymology, Vol. XI: Enzyme Structure (C. H. W. Hirs, ed.), p. 287, Academic Press, New York.
Codington, J. F., Sanford, B. H., and Jeanloz, R. W., 1970, Glycoprotein coat of the TA3 cell. L Removal of carbohydrate and protein material from viable cells, J. Natl. Cancer Inst. 45:637.
Codington, J. F., Sanford, B. H., and Jeanloz, R. W., 1972, Glycoprotein coat of the TA3 cell: Isolation and partial characterization of a sialic acid containing glycoprotein fraction. Biochemistry 11:2559.
Cook, G. M. W., 1968, Chemistry of membranes, Brit. Med. Bull. 24:118.
Cook, G. M. W., Heard, D. H., and Seaman, G. V. F., 1960, A sialomucopeptide liberated by trypsin from the human erythrocyte. Nature 188:1011.
Cuatrecasas, P., 1972a, Isolation of the insulin receptor of liver and fat-cell membranes, Proc. Natl. Acad. Sci. 69(2):318.
Cuatrecasas, P., 1972b, Affinity chromatography and purification of the insulin receptor of liver cell membranes, Proc. Natl. Acad. Sci. 69(5):1277.
Cuatrecasas, P., Wilcheck, M., and Anfinsen, C. B., 1968, Selective enzyme purification by affinity chromatography, Proc. Natl. Acad. Sci. 61:636.
Culp, L. A., and Black, P. H., 1972, Release of macromolecules from BALB/c mouse cell lines treated with chelating agents, Biochemistry 11:2161.
Cunningham, L. W., 1971, Microheterogeneity and function of glycoproteins, in: Glycoproteins of Blood Cells and Plasma (G. D. Jamieson and T. J. Greenwalt, eds.), pp. 16–34, Lippincott, Philadelphia.
Cunningham, L. W., and Ford, J. D., 1968, A comparison of glycopeptides derived from soluble and insoluble collagens, J. Biol. Chem. 243(9):2390.
Dische, Z.,and Shettles, L. B., 1948, A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination, J. Biol. Chem. 175:595.
Dodge, J. T., Mitchell, C., and Hanahan, D. J., 1963, The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys. 100:119.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F., 1956, Colorimetric method for the determination of sugars and related substances. Anal. Chem. 28:350.
Fairbanks, G., Steck, T. L., and Wallach, D. F. H., 1971, Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606.
Gahmberg, C. G., and Hakomori, S.-I., 1973, External labelling of cell surface Gal and GalNAc in glycolipid and glycoproteins: Patterns of normal vs. fetal erythrocytes and mouse 3T3 vs. 3T3 supy cells. Fed. Proc. 32:1711 (abst.).
Gesner, B. M., and Ginsberg, V., 1964, Effect of glycosidases on the fate of transfused lymphocytes, Proc. Natl. Acad. Sci. 52:750.
Ginsberg, V., 1972, ed.. Methods in Enzymology, Vol. XXVIII, Part B, Academic Press, New York.
Ginsberg, V., and Kobata, A., 1971, Structure and function of surface components of mammalian cells, in: Structure and Function of Biological Membranes (L. J. Rothfield, ed.), pp. 439–459, Academic Press, New York.
Ginsberg, V., and Neufeld, E., 1969, Complex heterosaccharides of animals, Ann. Rev. Biochem. 38:371.
Glick, D., 1955, ed., Methods of Biochemical Analysis, Vol. 2, Interscience, New York. Glick, M. C., 1971, Carbohydrate changes of mammalian cell membranes, Fed. Proc. 30(3):1044 (abst.).
Glick, M. C., and Buck, C. A., 1973, Glycoproteins from the surface of metaphase cells. Biochemistry 12:85.
Glick, M. C., Comstock, C., and Warren, L., 1970, Membranes of animal cells. VII. Carbohydrates of surface membranes and whole cells, Biochim. Biophys. Acta 219:290.
Glick, M. C., Gerner, E. W., and Warren, L., 1971, Changes in the carbohydrate content of the KB cell during the growth cycle. Cell Physiol 77:1.
Glick, M. C., Kimhi, Y., and Littauer, U. Z., 1973a, Glycopeptides from surface membranes of neuroblastoma cells, Proc. Natl. Acad. Sci. 70:1682.
Glick, M. C., Rabinowitz, Z., and Sachs, L., 1973b, Surface membrane glycopeptides correlated with tumorigenesis. Biochemistry 12:4864.
Glossmann, H., and Neville, D. M., Jr., 1971, Glycoproteins of cell surfaces: A comparative study of three different cell surfaces of the rat, J. Biol Chem. 246(20):6339.
Gottschalk, A., 1972, ed.. Glycoproteins, Their Composition, Structure and Function, Vols. I and II, American Elsevier, New York.
Gray, W. R., 1967, Sequential degradation plus dansylation, in: Methods in Enzymology, Vol. XI: Enzyme Structure (C. H. W. Hirs, ed.), pp. 469–475, Academic Press, New York.
Gray, W. R., 1972, End-group analysis using dansyl chloride, in: Methods in Enzymology, Vol. XXV: Enzyme Structure, Part B (C. H. W. Hirs and S. N. Timasheff, eds.), pp. 121–138, Academic Press, New York.
Green, D. E., 1972, ed., Membrane structure and its biological applications, Ann. N.Y. Acad. Sci. 195.
Greenberg, C. S., and Glick, M. C., 1972, Electrophoretic study of the polypeptides from surface membranes of mammalian cells. Biochemistry 11:3680.
Grimes, W. J., 1970, Sialic acid transferases and sialic acid levels in normal and transformed cells. Biochemistry 9:5083.
Gross, E., 1967, The cyanogen bromide reaction, in: Methods in Enzymology, Vol. XI: Enzyme Structure (C. H. W. Hirs, ed.), pp. 238–255, Academic Press, New York.
Guidotti, G., 1972, Membrane proteins, Ann. Rev. Biochem. 41:731.
Gwynne, J. T., and Tanford, C., 1970, A polypeptide chain of very high molecular weight from red blood cell membranes, J. Biol. Chem. 245:3269.
Hakomori, S.-I., 1964, A rapid permethylation of glycolipid and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide, J. Biochem. (Tokyo) 55:205.
Hamilton, P. B., 1967, Micro and submicro determinations of amino acids by ion-exchange chromatography, in: Methods in Enzymology, Vol. XI: Enzyme Structure (C. H. W. Hirs, ed.), pp. 15–27, Academic Press, New York.
Hartmann, J. P., Buck, C. A., Defendi, V., Glick, M. C., and Warren, L., 1972, The carbohydrate content of control and virus-transformed cells, J. Cell Physiol. 80:159.
Heller, J., and Lawrence, M. A., 1970, Structure of the glycopeptide from bovine visual pigment 500, Biochemistry 9(4):864.
Hickman, S., Kornfeld, R., Osterland, C. K., and Kornfeld, S., 1972, The structure of the glycopeptides of a human γM-immunoglobulin, J. Biol. Chem. 247(7):2156.
Hirs, C. H. W., 1967, ed.. Methods in Enzymology, Vol. XI: Enzyme Structure, Academic Press, New York.
Hirs, C. H. W., and Timasheff, S. N., 1972, eds.. Methods in Enzymology, Vol. XXV: Enzyme Structure, Part B, Academic Press, New York.
Huang, C.-C., Mayer, H. E., Jr., and Montgomery, R., 1970, Microheterogeneity and paucidispersity of glycoproteins. I. The carbohydrate of chicken ovalbumin, Carbohyd. Res. 13:127.
Hughes, R. C., Sanford, B., and Jeanloz, R. W., 1972, Regeneration of the surface glycoproteins of a transplantable mouse tumor cell after treatment with neuraminidase, Proc. Natl Acad. Scl 69(4):942.
Jakoby, R. K., and Warren, L., 1961, Identification and quantitation of N-acetylneuraminic acid in human cerebrospinal fluid. Neurology 11:232.
Jamieson, G. A., and Greenwalt, T. J., 1969, eds., Red Cell Membranes, Lippincott, Philadelphia.
Jamieson, G. A., and Greenwalt, T. J., eds., 1971, Glycoproteins of Blood Cells and Plasma, Lippincott, Philadelphia.
Jamieson, G. A., Jett, M., and DeBernardo, S. L., 1971, The carbohydrate sequence of the glycopeptide chains of human transferrin, J. Biol. Chem. 246(11):3686.
Jansons, V. K., and Burger, M. M., 1973, Isolation and characterization of agglutinin receptor sites. II. Isolation and partial purification of a surface membrane receptor for wheat germ agglutinin, Biochim. Biophys. Acta 291:127.
Jansons, V. K., Sakamoto, C. K., and Burger, M. M., 1973, Isolation and characterization of agglutinin receptor sites. III. Studies of the interaction with other lecitins, Biochim. Biophys. Acta 291:136.
Jeanloz, R. J., 1969, ed.. The Amino Sugars, Academic Press, New York.
Kathan, R. H., Winzler, R. J., and Johnson, C. A., 1961, Preparation of an inhibitor of viral hemagglutination from human erythrocytes, J. Exptl. Med. 113:37.
Kohno, M., and Yamashina, I., 1972, Purification and properties of 4-l-aspartylglycosylamine and amidohydrolase from hog kidney, Biochim. Biophys. Acta 258:600.
Korn, E. D., 1969, Cell membranes: Structure and synthesis, Ann. Rev. Biochem. 38:263.
Kornfeld, R., and Kornfeld, S., 1970, The structure of a phytohemagglutinin receptor site from human erythrocytes, J. Biol. Chem. 245(10):2536.
Kornfeld, R., Keller, J., Baenziger, J., and Kornfeld, S., 1971, The structure of the glycopeptide of human γG myeloma proteins, J. Biol. Chem. 246(10):3259.
Kornfeld, S., and Kornfeld, R., 1969, Solubilization and partial characterization of a phytohemagglutinin receptor site from human erythrocytes, Proc. Natl. Acad. Sci. 63(4):1439.
Kornfeld, S., Rogers, J., and Gregory, W., 1971, The nature of the cell surface receptor site for Lens culinaris phytohemagglutinin, J. Biol. Chem. 246(21):6581.
Kraemer, P. M., 1971, Complex carbohydrates of animal cells: Biochemistry and physiology of the cell periphery, in: Biomembranes I (L. Manson, ed.), pp. 67–190, Plenum Press, New York.
Lee, Y. C., McKelvy, J. F., and Lang, D., 1969, Rapid automatic analysis of sugar components of glycoproteins. II. Neutral sugars. Anal. Biochem. 27:561.
Lehnhardt, W. F., and Winzler, R. J., 1968, Determination of neutral sugars in glycoproteins by gas-liquid chromatography, J. Chromatog. 34:471.
Li, S.-C., and Li, Y.-T., 1970, Studies on the glycosidases of jack bean meal. III. Crystallization and properties of β-N-acetylhexosaminidase, J. Biol. Chem. 245(19):5153.
Li, Y.-T., 1967, Studies on the glycosidases in jack bean meal. I. Isolation and properties of α-mannosidase, J. Biol. Chem. 242(23):5474.
Li, Y.-T., and Li, S.-C., 1968, Studies on the glycosidases in jack bean meal. II. Separation of various glycosidases by iso-electric focusing, J. Biol. Chem. 243(14):3994.
Li, Y.-T., Li, S.-C., and Shetlar, M. R., 1968, Isolation of glycopeptides from rat liver microsomes involved in the biosynthesis of plasma glycoprotein, J. Biol. Chem. 243(3):656.
Liu, T.-Y., 1973, Determination of sialic acid using an amino acid analyzer, in: Methods in Enzymology, Vol. XXVIII: Complex Carbohydrates, Part B (V. Ginsberg, ed.), pp. 48–53, Academic Press, New York.
Makino, M., Kojima, T., and Yamashina, I., 1966, Enzymatic cleavage of glycopeptides, Biochem. Biophys. Res. Commun. 24(6):961.
Marchesi, V. T., and Andrews, E. P., 1971, Glycoproteins: Isolation from cell membranes with lithium diiodosalicylate. Science 174:1247
Marchesi, V. T., Tillack, T. W., Jackson, R. L., Segrest, J. P., and Scott, R. E., 1972, Chemical characterization and surface orientation of the major glycoprotein of the human erythrocyte membrane, Proc. Natl. Acad. Sci. 69(6):1445.
Marshall, R. D., 1972, Glycoproteins, Ann. Rev. Biochem. 41:673.
Melchers, P., and Andersson, J., 1973, Synthesis, surface deposition and secretion of immunoglobulin M in bone marrow-derived lymphocytes before and after mitogenic stimulation. Transplant. Rev. 14:76.
Molnar, J., 1967, Glycoproteins of Ehrlich ascites carcinoma cells: Incorporation of (14C) glucosamine and (14C) sialic acid into membrane proteins. Biochemistry 6:3064.
Morell, A. G., Van Den Hamer, C. J. A., and Scheinberg, I. H., 1966, Physical and chemical studies on ceruloplasmin. IV. Preparation of radioactive, sialic acid-free ceruloplasmin labeled with tritium on terminal d-galactose residues, J. Biol. Chem. 241(16):3745.
Muramatsu, T., 1971, Demonstration of an endo-glycosidase acting on a glycoprotein, J. Biol. Chem. 246:5535.
Muramatsu, T., and Nathenson, S. G., 1970, Studies on the carbohydrate portion of membrane-located mouse H-2 alloantigen. Biochemistry 9:4875.
Nathenson, S. G., Shimada, A., Yamane, K., Muramatsu, T., Gullen, S., Mann, D. L., Pahey, J. L., and Graff, R., 1970, Biochemical properties of papain-solubilize murine and human histocompatibility alloantigens. Fed. Proc. 29(6):2026.
Neuberger, A., 1971, Past and present concept of glycoproteins, in: Glycoproteins of Blood Cells and Plasma (G. D. Jamieson and T. J. Greenwalt, eds.), pp. 1–15, Lippincott, Philadelphia.
Neuberger, A., and Marshall, R. D., 1968, in: Carbohydrates and Their Roles (H. W. Schultze, R. F. Cain, and R. W. Wrotstad, eds.), Avi, Westport, Conn.
Neville, D. M., Jr., 1960, The isolation of a cell membrane fraction from rat liver, J. Biophys. Biochem. Cytol. 8:413.
Niederwieser, A., 1972, Thin-layer chromatography of amino acids and derivatives, in: Methods in Enzymology, Vol. XXV: Enzyme Structure, Part B (C. H. W. Hirs and S. N. Timasheff, eds.), pp. 60–99, Academic Press, New York.
Oppenheimer, S. B., Edidin, M., Orr, C. W., and Roseman, S., 1969, An l-glutamine requirement for intercellular adhesion, Proc. Natl. Acad. Sci. 63:1395.
Osborn, M. J., 1971, The role of membranes in the synthesis of macromolecules, in: Structure and Function of Biological Membranes (L.I. Rothfield, ed.), pp. 343–400, Academic Press, New York.
Pepper, D. S., and Jamieson, G. A., 1970, Isolation of a macroglycopeptide from human platelets. Biochemistry 9:3706.
Phillips, D. R., 1972, Effect of trypsin on the exposed polypeptides and glycoproteins in the human platelet membrane. Biochemistry 11:4582.
Phillips, D. R., and Morrison, M., 1971, Exposed protein on the intact human erythrocyte. Biochemistry 10:1766.
Pigman, W., and Horton, D., 1970, The Carbohydrates, Chemistry and Biochemistry, Vols. IIA and IIB, Academic Press, New York.
Poduslo, J. F., Greenberg, C. S., and Glick, M. C., 1972, Proteins exposed on the surface of mammalian membranes, Biochemistry 11:2616.
Ponder, E., 1952, Various types of ghosts derived from human red cells: Heat fragmentation and plasma optics studies, J. Exptl Biol. 29:605.
Presant, C. A., and Kornfeld, S., 1972, Characterization of the cell surface receptor for the Agaricus bisporus hemagglutinin, Biol. Chem. 247(21):6937.
Reinhold, V. N., 1972, Gas-liquid chromatographic analysis of constituent carbohydrates in glycoproteins, in: Methods in Enzymology, Vol. XXV: Enzyme Structure, Part B (C.H.W. Hirs and S.N. Timasheff, eds.), pp. 244–249, Academic Press, New York.
Roe, J. H., 1955, The determination of sugar in blood and spinal fluid with anthrone reagent, J. Biol. Chem. 212:335.
Rosai, J., Tillack, T. W., and Marchesi, V. T., 1971, A new method for the isolation of tumor-specific antigens from human neoplasms. Fed. Proc. 30(2):453 (abst.).
Roth, S., and White, D., 1972, Intercellular contact and cell-surface galactosyl transferase activity, Proc. Natl. Acad. Sci. 69(2):485.
Rothfield, L. J., 1971, ed., Structure and Function of Biological Membranes, Academic Press, New York.
Sakiyama, H., and Bürge, B. W., 1972, Comparative studies of the carbohydrate-containing components of 3T3 and simian virus 40 transformed 3T3 mouse fibroblasts. Biochemistry 11:1366.
Sharon, N., and Lis, H., 1972, Lectins: Cell-agglutinating and sugar-specific proteins. Science 177(4053):949.
Sharon, N., and Lis, H., 1973, The biochemistry of plant lectins (phytohemagglutinins), Ann. Rev. Biochem. 42:541.
Sheinin, R., and Onodera, K., 1970, Studies on the biochemical properties of surface component of normal and SV-40 transformed 3T3 mouse cells. Can. J. Biochem. 48:851.
Shen, L., and Ginsberg, V., 1968, Release of sugars from HeLa cells by trypsin, in: Biological Properties of the Mammalian Surface Membrane (L. A. Manson, ed.), pp. 67–71, Wistar Institute Symposium Monograph No. 8.
Shimizu, S., and Funakoshi, I., 1970, Carbohydrate composition of the plasma membrane of rat ascites hepatoma, Biochim. Biophys. Acta 203:167.
Slayter, H. S., and Codington, J. F., 1973, Size and configuration of glycoprotein fragments cleaved from tumor cells by proteolysis, J. Biol. Chem. 248(10):3405.
Smith, D. F., and Walborg, E. F., Jr., 1972, Isolation and chemical characterization of cell surface sialoglycopeptide fractions during progression of rat ascites hepatoma AS-30D, Cancer Res. 32:543.
Smith, D. F., Neri, G., and Walborg, E. F., Jr., 1973, Isolation and partial chemical characterization of cell-surface glycopeptides from AS-30D rat hepatoma which possess binding sites for wheat germ agglutinin and concanavalin A, Biochemistry 12(11):2111.
Smith, F., and Unrau, A. M., 1959, On the presence of 1←6 linkages in lamarin, Chem. Ind. 881.
Somogyi, M., 1952, Notes on sugar determination, J. Biol. Chem. 195:19.
Spiro, R. G., 1964, Periodate oxidation of the glycoprotein fetuin, J. Biol. Chem. 239(2):567.
Spiro, R. G., 1966, Analysis of sugars found in glycoproteins: Characterization of carbohydrate units of glycoproteins, in: Methods in Enzymology, Vol. VIII: Complex Carbohydrates (E. Neufeld and V. Ginsberg, eds.), pp. 3–52, Academic Press, New York.
Spiro, R. G., 1967, Studies on the renal glomerular basement membrane: Nature of the carbohydrate units and their attachment to the peptide portion, J. Biol. Chem. 242(8):1923.
Spiro, R. G., 1970, Glycoproteins, Ann. Rev. Biochem. 39:599.
Spiro, R. G., and Fukushi, S., 1969, The lens capsule: Studies on the carbohydrate units, J. Biol. Chem. 244(8):2049.
Sukeno, T., Tarentino, A. L., Plummer, T. H., Jr., and Maley, P., 1971, On the nature of α-mannosidase-resistant linkages in glycoproteins, Biochem. Biophys. Res. Commun. 45:219.
Svennerholm, E., and Svennerholm, L., 1958, Quantitative paper partition chromatography of sialic acids. Nature 181:1154.
Sweeley, C. C., Wells, W. W., and Bentley, R., 1966, Gas chromatography of carbohydrates, in: Methods in Enzymology, Vol. VIII: Complex Carbohydrates (E. Neufeld, and V. Ginsberg, eds.), pp. 95–108, Academic Press, New York.
Tarentino, A. L., and Maley, P., 1969, The purification and properties of a β-aspartyl N-acetylglucosylamine amidohydrolase from hen oviduct. Arch. Biochem. Biophys. 130:295.
Tarentino, A., Plummer, T. H., Jr., and Maley, F., 1970, Studies on the oligosaccharide sequence of ribonuclease B, J. Biol. Chem. 245:4150.
Thomas, D. B., and Winzler, R. J., 1969a, Oligosaccharides from M-active sialoglyco-peptides of human erythrocytes, Biochem. Biophys. Res. Commun. 35(6):811.
Thomas, D. B., and Winzler, R. J., 1969b, Structural studies of human erythrocyte glycoproteins: Alkali-labile oligosaccharides, J. Biol. Chem. 244(21):5943.
Trevelyan, W. E., Procter, D. P., and Harrison, J. S., 1950, Detection of sugars on paper chromatograms. Nature 166:445.
Van Lenten, L., and Ashwell, G., 1971, Studies on the chemical and enzymatic modification of glycoproteins: A general method for the tritation of sialic acid-containing glycoproteins, J. Biol. Chem. 246(6):1889.
Walborg, E. P., Jr., and Kondo, L., 1970, Automated system for ion-exchange chromatography of saccharides. Anal. Biochem. 37:320.
Walborg, E. P., Jr., Cobb, B. F., Adams-Mayne, M., and Ward, D. N., 1963, Semiautomatic analysis of glucosamine and galactosamine in protein hydrolyzates, Anal. Biochem. 6:367.
Walborg, E. P., Jr., Lantz, R. S., and Wray, V. P., 1969a, Isolation and chemical characterization of a cell-surface sialoglycopeptide fraction from Novikoff ascites cells, Cancer Res. 29:2034.
Walborg, E. P., Jr., Ray, D. B., and Öhrberg, L. E., 1969b, Ion-exchange chromatography of saccharides: An improved system utilizing boric acid/2,3-butanediol buffers. Anal. Biochem. 29:433.
Wallach, D. P. H., and Kamat, V. B., 1966, Preparation of plasma-membrane fragments from mouse ascites tumor cells, in: Methods in Enzymology, Vol. VIII: Complex Carbohydrates (E. Neufeld and V. Ginsberg, eds.), pp. 164–172, Academic Press, New York.
Warren, L., 1959, The thiobarbituric acid assay of sialic acids, J. Biol. Chem. 234:1971.
Warren, L., and Glick, M. C., 1969, Isolation of surface membranes of tissue culture cells, in: Fundamental Techniques in Virology (K. Habel and N. P. Salzman, eds.), pp. 66–71, Academic Press, New York.
Warren, L., and Glick, M. C., 1971, The isolation of the surface membranes of animal cells: A survey, in: Biomembranes I (L. A. Manson, ed.), pp. 257–288, Plenum Press, New York.
Warren, L., Critchley, D., and Macpherson, I., 1972a, Surface glycoproteins and glycolipids of chicken embryo cells transformed by a temperature-sensitive mutant of Rous sarcoma virus, Nature 235:275.
Warren, L., Fuhrer, J. P., and Buck, C. A., 1972b, Surface glycoproteins of normal and transformed cells: A difference determined by sialic acid and a growth-dependent sialyl transferase, Proc. Natl. Acad. Sci. 69:1838.
Weber, P., and Winzler, R. J., 1969, Determination of hexosaminitols by ion-exchange chromatography and its application to alkali-labile glycosidic linkages in glycoproteins, Arch. Biochem. Biophys. 129:534.
Weinstein, D. B., Marsh, J. B., Glick, M. C., and Warren, L., 1970, Membranes of animal cells. VI. The glycolipids of the L cell and its surface membranes, J. Biol. Chem. 245:3928.
Weissmann, B., and Hinrichsen, D. F., 1969, Mammalian α-acetylgalactosaminidase: Occurrence, partial purification, and action on linkages in submaxillary mucins. Biochemistry 8:2034.
Winzler, R. J., 1969, in: Red Cell Membranes (G. A. Jamieson and T. J. Greenwalt, eds.), pp. 157–171, Lippincott, Philadelphia.
Winzler, R. J., 1970, Carbohydrates in cell surfaces, Internat. Rev. Cytol. 29:77.
Wu, H. C., Meezan, E., Black, P. H., and Robbins, P. W., 1969, Comparative studies on the carbohydrate containing membrane components of normal and virus-transformed mouse fibroblasts. I. Glucosamine labeling patterns in 3T3, spontaneously transformed 3T3, and SV-40 transformed 3T3 cells, Biochemistry 8:2509.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Plenum Press, New York
About this chapter
Cite this chapter
Glick, M.C. (1974). Isolation and Characterization of Surface Membrane Glycoproteins from Mammalian Cells. In: Korn, E.D. (eds) Methods in Membrane Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8960-0_2
Download citation
DOI: https://doi.org/10.1007/978-1-4615-8960-0_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-8962-4
Online ISBN: 978-1-4615-8960-0
eBook Packages: Springer Book Archive