Abstract
In Chapter 7 we discussed the mechanisms by which solvents interact with fluorophores, and the effects of these interactions on emission spectra. These emission spectra can be shifted to lower energy either as a result of the general solvent effects, or by specific effects based on chemical interactions between the fluorophore and the solvent. Throughout this discussion we assumed that the fluorophore was in a fluid solvent, and that the relaxation time of the solvent (τ s ) was much smaller than the fluorescence lifetime (τ). As a result, emission occurred from the relaxed state where the solvent was in equilibrium with the new electron distribution of the excited fluorophore. The reorganization of the solvent around the excited state dipole is an excited state reaction. In Chapter 12 we will describe the effects of a simple two-state reaction on the spectral properties of fluorophores. In this instance, the time-resolved decays of fluorescence, and the phase-modulation data, display a number of general and useful characteristics. Once understood, these characteristics can be used to prove that an excited state reaction is occurring, and to derive the kinetic constants for the system. The two-state model is relatively simple in that there are only two emitting species, and the lifetimes of these species are independent of emission wavelength. In many respects, the two-state model provides a useful conceptual framework within which to consider any excited state process.
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© 1983 Plenum Press, New York
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Lakowicz, J.R. (1983). Mechanisms and Dynamics of Solvent Relaxation. In: Principles of Fluorescence Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7658-7_8
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DOI: https://doi.org/10.1007/978-1-4615-7658-7_8
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