Abstract
Recent advances in the purification of DNA-binding proteins have been due primarily to the use of the specific sequence of the binding site as a ligand for affinity chromatography. Different variations of this basic principle have been developed in several laboratories, and all have been found to function effectively.
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References
Rosenfeld, P.J. and Kelly, T.J. (1986) J. Biol. Chem. 261, 1398–1408.
Kadonaga, J.T. and Tjian, R. (1986) Proc. Nat. Acad. Sci.U.S.A. 83, 5889–5893.
Wu, C., Wilson, S., Walker, B., Dawid, I., Paisley, T.,Zimarino, V. and Ueda, H. (1987) Science 238, 1247–1253.
Bahi,C.P.,Wu,R.and narang,S.A. (1980) Methods Enzymol. 65, 877–885.
Galas, D.J. and Schmitz, A. (1978) Nucl. Acids Res. 5, 3157–3170.
Arndt-Jovin, D.J., Jovin, T.M., Bahr, W., Frischauf, A.M. and Maequardt, M. (1975) Eur. J. Biochem. 54, 411–418.
Bunemann,H., Westhoff, P. and Herrmann, R.G. (1982) Nucl. Acids Res. 10, 7163–7180.
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© 1988 Plenum Press, New York
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Wu, C., Tsai, C., Wilson, S. (1988). Affinity Chromatography of Sequence-Specific DNA-Binding Proteins. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7081-3_3
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DOI: https://doi.org/10.1007/978-1-4615-7081-3_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-7083-7
Online ISBN: 978-1-4615-7081-3
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