Abstract
The application of NMR methods for the investigation of metabolic processes in living systems is now well established. In recent years 31P-NMR in particular has provided a wealth of information regarding the flux of phosphorylated metabolites under physiological conditions in whole cells, isolated organs, and within intact organisms. These studies have been extensively reviewed (Burt et al., 1979; Radda and Seeley, 1979; Gadian and Radda, 1981). Despite this success in the elucidation of cellular energetic processes 31P-NMR suffers intrinsic limitations as a metabolic probe. Firstly, many processes of interest do not directly involve net changes of concentration of phosphorylated species, and secondly since the 31P nucleus is present at 100% natural abundance the fate of a particular atom cannot be monitored. Although a phosphorylated metabolite may be undergoing rapid turnover this may not be readily apparent since the method is sensitive only to changes in overall concentration. An exception to this is the application of saturation transfer methods which have provided valuable kinetic information on the exchange rates between ATP and Pi and phosphocreatine and ATP in vivo (Brown et al., 1977; Gadian et al., 1981).
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© 1983 Plenum Press, New York
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Baxter, R.L., Mackenzie, N.E., Scott, A.I. (1983). CMR as a Probe for Metabolic Pathways in vivo . In: Berliner, L.J., Reuben, J. (eds) Biological Magnetic Resonance. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6543-7_1
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