Abstract
We have sequenced that part of the spike protein (S) gene which encodes the amino-terminal and most variable quarter (hypervariable region, HVR) of the SI subunit of 28 isolates of the 793/B (also known as CR88 and 4/91) serotype of infectious bronchitis virus (IBV) and the whole of S1 for nine of them. The isolates were from France and Britain between the years 1985 (first isolation) and 1996. The maximum nucleotide and amino acid differences between the first isolate and the others were 4.1% and 7.6%, respectively, for the whole of SI and 7.1% and 14.6%, respectively, in the HVR. Analysis within clearly recognisable subgroups suggested that even in the HVR the nucleotide mutation rate was only 0.3 to 0.6% per year. However, there was no evidence that mutations had become fixed in a progressive manner; this serotype did not appear to be evolving. Strains isolated several years apart could be more similar than those isolated in a given year. It is likely that the amino acid changes are largely at positions where amino acid differences are tolerated rather than as a consequence of immune pressure. Reasons for this conclusion are discussed.
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References
A. Adzhar, R. E. Gough, D. Haydon, K. Shaw, P. Britton & D. Cavanagh., 1997, Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great Britain. Avian Pathol. 27. In press.
Cavanagh, D., 1995, The Coronavirus surface glycoprotein, in: S.G. Siddell (Ed) The Coronaviridae, 73–114, New York, Plenum Press.
Cook, J.K.A., Orbell, S.J., Woods, M.A. & Huggins, M.B., 1996, A survey of the presence of a new infectious bronchitis virus designated 4/91 (793B). Vet. Rec. 138: 178–180.
Felsentein, J., 1993, PHYLIP (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle.
Gough, R.E., Randall, C.J., Dagless, M., Alexander, D.J., Cox, W.J. & Pearson, D., 1992, A ‘new’ strain of infectious bronchitis virus infecting domestic fowl in Great Britain. Vet. Rec. 130:493.
Hughes, M.K. & Hughes, A.L., 1995, Natural selection on Plasmodium surface proteins. Mol Biochem. Parasitol. 71:99–113.
Jukes, T.H. & Cantor, CR., 1969, Evolution of protein molecules, in: Munro, H.N. (Ed) Mammalian Protein Metabolism, 21–132.
Kant, A., Koch, G., Van Roozelaar, D.J., Küsters, J.G., Poelwijk, F.A.J. & Van Der Zeijst, B.A.M., 1992, Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide. J. Gen. Virol. 73: 591–596.
Nei, M. & Gojobori, T., 1986, Simple methods for estimating the numbers of synonymous and non-synonymous nucleotide substitutions. Mol. Biol. Evol. 3: 418–426.
Parsons, D., Ellis, M.M., Cavanagh, D. & Cook, J.K.A., 1992, Characterisation of an infectious bronchitis virus isolated from vaccinated broiler breeder flocks. The Vet. Rec. 131: 408–411.
Picault, J.P., Drouin, P., Lamande, J., Allee, C., Toux, J.Y., Le Coq, H., Guittet, M. & Bennejean, G., 1995, L‘epizootie recente de bronchite infectieuse aviaire en France: importance, evolution et etiologie. 1eres Journee de la Recherche Avicole, Centre de Congres, d’Angers.
Seibert, S.A., Howell, C.Y., Hughes, K.A., & Hughes, K.L., 1995, Natural selection on the gag, pol and env genes of human immunodeficiency virus 1 (HIV-1). Mol. Biol. Evol., 12: 803–813.
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Cavanagh, D. et al. (1998). Does IBV Change Slowly Despite the Capacity of the Spike Protein to Vary Greatly?. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_94
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_94
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