Abstract
Over the past two decades there has been increased interest in the possibility of cellular damage and dysfunction caused by oxidative biochemical reactions involving oxygen by products, particularly oxygen-derived free radicals and nitric oxide. Free radicals have been suggested as playing an important role in a wide variety of clinical diseases including heart attack, stroke, respiratory distress syndrome, acute tubular necrosis of kidney, reperfusion injury of a wide variety of organs, and oncogenesis and tumor promotion (Korthius & Granger, 1986; Fantone & Ward, 1982; Guarnieri et al., 1980; Hess & Manson, 1984; Burton et al., 1984; Fridovich, 1983, 1989). In certain cases such as radiation injury and some chemical toxicities free radical injury has been considered to be the sole cause. Thus, free radicals have been proposed to mediate many of the most prevalent diseases causing morbidity and mortality. Of the several methods available to study free radicals in biological tissues and cells, electron paramagnetic resonance (EPR) spectroscopy has been recognized to be the most important and direct technique.
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Zweier, J.L., Kuppusamy, P. (1998). Principles of Electron Paramagnetic Resonance Spectroscopy for Measurement of Free Radicals in Biological Tissues. In: Lukiewicz, S., Zweier, J.L. (eds) Nitric Oxide in Transplant Rejection and Anti-Tumor Defense. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5081-5_2
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DOI: https://doi.org/10.1007/978-1-4615-5081-5_2
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