Abstract
The measurement of oxygen consumption in small tissue regions has been problematic. The perfusion of myocardium is very heterogeneous,1 and it was desirable to measure whether aerobic metabolic rate paralleled the blood flow heterogeneity. However, no method was available to measure local oxygen consumption in tissue regions of a similar size as used for measurement of local blood flow with labeled microspheres. Our goal was to develop a method to measure local O2 consumption (VO2) in many tissue samples simultaneously. VO2 is proportional to the rate in the tricarboxylic acid (TCA) cycle. Methods to determine the flux in the TCA cycle by measuring the gradual enrichment of glutamate with 13C isotopes with NMR spectroscopy exist.2–6 NMR spectra are measured every few minutes until a steady state has been reached, and the time course of enrichment is analyzed with a model for the isotope distribution in the TCA cycle.2 Local blood flow is measured in many small regions simultaneously, and VO2 too must be assessed in many regions simultaneously, which is not yet feasible with NMR coils in vivo. Our strategy is to freeze tissue sam ples quickly at a prescribed time briefly after starting infusion of 13C-enriched substrate for the TCA cycle (e.g. acetate, pyruvate, lactate) and to analyze the 13C NMR multiplets of glutamate in extracts of the samples. We will show below that measurement of O2 consumption is feasible in this way, and test the method in isolated perfused rabbit hearts.
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van Beek, J.H.G.M., Csont, T., de Kanter, F.J.J., Bussemaker, J. (1998). Simple Model Analysis of 13C NMR Spectra to Measure Oxygen Consumption Using Frozen Tissue Samples. In: Hudetz, A.G., Bruley, D.F. (eds) Oxygen Transport to Tissue XX. Advances in Experimental Medicine and Biology, vol 454. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4863-8_58
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DOI: https://doi.org/10.1007/978-1-4615-4863-8_58
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