Abstract
In mammalian cells, prostaglandin (PG)-biosynthetic pathways utilizing endogenous arachidonic acid (AA) are subdivided into three distinct phases, which show different kinetics and may recruit different sets of biosynthetic enzymes. The constitutive immediate response, which occurs within several minutes after stimuli causing a rapid and transient increase in cytoplasmic Ca2+, is regulated by post-translational activation of constitutively expressed enzymes. The delayed response, which proceeds gradually for several hours after proinflammatory stimuli, requires de novo synthesis of particular biosynthetic enzymes. The induced immediate response, which is elicted by Ca2+-mobilizing stimuli after priming by proinflammatory stimuli, reflects the combination of the above two responses, and involves both constitutive and inducible enzymes. Here we introduce the differential coupling of the initial [phospholipase A2 (PLA2)], intermediate [cyclooxygenase (COX)], and terminal [terminal PG synthase (tPGS)] biosynthetic enzymes in each phase using rat peritoneal macrophages, mouse osteoblasts and other cell types as model systems.
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Kudo, I., Murakami, M. (1999). Diverse Functional Coupling of Prostanoid Biosynthetic Enzymes in Various Cell Types. In: Honn, K.V., Marnett, L.J., Nigam, S., Dennis, E.A. (eds) Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury, 4. Advances in Experimental Medicine and Biology, vol 469. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4793-8_5
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DOI: https://doi.org/10.1007/978-1-4615-4793-8_5
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