Abstract
Recombinant egg white avidin and bacterial B-glucuronidase (GUS) from transgenic maize have been commercially produced. High levels of expression were obtained in seed by employing the ubiquitin promoter from maize. The recombinant proteins had activities that were indistinguishable from their native counterparts. We have illustrated that downstream activities in the production of these recombinant proteins, such as stabilizing the germplasm and processing for purification, were accomplished without any major obstacles. Avidin (A8706) and GUS (G2035) are currently marketed by Sigma Chemical Co.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
An, G., Mitra, A., Choi, H.K., Costa, M.A., An, K., Thornburg, R.W., Ryan, CA. Functional analysis of the 3’ control region of the potato wound-inducible Proteinase inhibitor II gene. Plant Cell 1989, 1, 115–122.
Austin, S., Bingham, E.T., Koegel, R.G., Mathews, D.E., Shahan, M.N., Straub, R.J., Burgess, R.R. An overview of a feasibility study for the production of industrial enzymes in trangenic alfalfa. Ann. NY Acad. Sci. 1994, 727,234–244.
Benfey, P.N., Chua, N-H. Regulated genes in transgenic plants. Science 1989, 244, 174–181.
Brot, F.E., Bell, C.E., Sly, W.S. Purification and properties of β-glucuronidase from human placenta. Biochemistry 1978, 17,385–391.
Christensen, A.M., Sharrock, R.A., Quail, P.H. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol Biol. 1992,18, 675–689.
Clements, J.M., O’Connel, L.I., Tsunasawa, S., Sherman, F. Expression and activity of a gene encoding rat cyto-chrome c in the yeast Saccharomyces cerevisiae. Gene 1989, 83, 1–14.
Cornejo, M., Luth, D., Blankenship, K., Anderson, O., Blechl, A. Activity of a maize ubiquitin promoter in transgenic rice. Plant Mol. Biol. 1993, 23, 567–581.
DeLange, R.J., Huang, T.S. Egg White Avidin III. Sequence of the 75-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit. J. Biol.Chem. 1971, 246, 698–709.
Fisk, H.J., Dandekar, A.M. The introduction and expression of transgenes in plants. Scientia Hort. 1993, 55, 5–36.
Gehrmann, M.C., Opper, M., Sedlacek, H.H., Bosslet, K., Czech, J. Biochemical properties of recombinant human β-glucuronidase synthesized in baby hamster kidney cells. Biochem J. 1994, 301, 821–828.
Gope, M.L., Keinanen, R.A., Kristo, P.A., Conneely, O.M., Beattie, W.G., Zarucki-Schulz, T., O’Malley, B.W., Kulomaa, M.S.: Molecular cloning of the chicken avidin cDNA. Nuc. Acids Res. 1987, 15, 3595–3606.
Heney, G., Orr, G.A.. The purification of avidin and its derivatives on 2-iminobiotin-6-aminohexyl-sepharose 4B. Anal Biochem. 1981, 114, 92–96.
Hiller, Y., Gershoni, J.M., Bayer, E.A., Wilchek, M. Biotin binding to avidin. Biochem J. 1987, 248, 167–171.
Hirel, P-H., Schmitter, J-M., Dessen, P., Fayat, G., Blanquet, S. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimat amino acid. Proc Natl. AcadSci USA, 1989, 86, 8247–8251.
Ho, K-J. A large scale purification of β-glucuronidase from human liver by immunoaffinity chromatography. Biotech and Appl Biochem 1991, 14, 296–305.
Hood, E.E., Witcher, D.R., Maddock, S., Meyer, T., Baszczynski, C., Bailey, M., Flynn, P., Register, J., Marshall, L., Bond, D., Kulisek, E., Kusnadi, A., Evangelista, R., Nikolov, Z., Wooge, C., Mehigh, R.J., Hernán, R., Kappel, W.K., Ritland, D., Li, C.P., Howard, LA. Commercial production of avidin from transgenic maize: Characterization of transformant, production, processing, extraction and purification. Mol. Breeding 1997, 3:291–306.
Holzman, D. Agracetus grows monoclonals in soybeans and corn plants. Genet. Eng. News 1994, 14(16), 1, 34.
Jefferson, R.A., Burgess, S.M., Hirsch, D. β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci 1986, 83, 8447–8451.
Jefferson, R.A. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Molec Biol Rep 5, 1987, 387–405.
Jefferson, R.A., Kavanagh, T.A., Bevan, M.W. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J, 1987, 6, 3901–3907.
Jhingan, A.K. A novel technology for DNA isolation. Methods Mol Cell Biol, 1992, 3, 15–22.
Keinanen, R.A., Laukkanen, M-L., Kulomaa, M.S. Molecular cloning of three structurally related genes for chicken avidin. J. Steroid Biochem. 1988, 30, 17–21.
Keinanen, RA., Wallen, M.J., Kristo, P.A., Laukkanen, M.O., Toimela, T.A., Helenius, M.A., Kulomaa, M.S. Molecular cloning and nucleotide sequence of chicken avidin-related genes 1-5. Eur. J. Biochem. 1994, 220, 615–621.
Krebbers, E., Bosch, D., Vandekerckhove, J. Prospects and progress in the production of foreign proteins and pep-tides in plants. In Plant Protein Engineering Shewry, P.R., Gutteridge, S., Eds.; Cambridge University Press, pp.315–325 Cambridge, MA 1993.
Kusnadi, A.R., Hood, E.E., Witcher, D.R., Howard, J.A., Nikolov, Z.L. Production and purification of two recombinant proteins from transgenic corn. Biotechnology Progress. 1998,14, 147–155.
Matsudaira, P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride mem-brnes. J. Biol. Chem. 1987, 262, 10035–10038.
Nagy, F., Odell, J.T., Morelli, G., Chua, N.H. Properties of expression of the 35S promoter from CaMV in transgenic tobacco plants. In Biotechnology in plant science: relevance to agriculture in the eighties. Zaitlin, M., Day, P., Hollaender, A. Eds.; Academic Press, p. 227–235, Orlando, FL 1985.
Pen, J., Verwoerd, T.C., van Paridon, P.A., Beudeker, R.F., van den Elzen, P.J.M., Geerse, K., van der Klis, J.D., Versteegh, H.A.J., van Ooyen, A.J.J., Hoekema, A. Phytase-containing transgenic seeds as a novel feed additive for improved phosphorus utilization. Bio/Technol, 1993A, 11, 811–814.
Pen, J., Sijmons, P.C., van Ooijen, A.J.J., Hoekema, A. Protein production in transgenic crops: Analysis of plant molecular farming. In Industrial Crops Production. Elsevier, Amsterdam, pp. 241–250 1993B.
Rogers, J.C. Two barley alpha-amylase gene families are regulated differently in aleurone cells. J. Biol. Chem. 1985,250,3731–3738.
Rethinaswamy, A., Yang, C-H., Srivastava, P.N. Purification and characterization of β-glucuronidase from bull seminal plasma and its role in fertilization. Mol Reprod and Dev. 1994, 38, 404–409.
Schmidt, J., Herfurth, E., Subramanian, A.R. Purification and characterization of seven chloroplast ribosomal proteins: Evidence that organeile ribosomal protein genes are functional and that NH2-terminal processing oc-curs via multiple pathways in chloroplasts. Plant Mol Biol. 1992, 20, 459–465.
Termignoni, C., Freitas, J.O., Guimaraes, J.A. Methionyl aminopeptidase from rat liver: distribution of the membrane-bound subcellular enzyme. Mol Cell Biochem., 1991,102, 101–113.
White J., Chang, S-Y.P., Bibb, M.J., Bibb, J.M. A cassette containing the bar gene of Streptomyces hygroscopicus: a selectable marker for plant transformation. Nuc. Acids Res. 1990,18, 1062.
Whitelem, G.C., Cockburn, B., Gandecha, A.R., Owen, M.R.L. Heterologous protein production in transgenic plants. Biotechnol. Genetic Eng. Rev. 1993, 11, 1–29.
Wilson, C.M. Proteins of the kernel. In Corn: chemistry and technology. Watson, S.A., Ramstad, P.E. Eds., American Association of Cereal Chemists, Inc. St. Paul, MN 1987, pp. 273–305.
Witcher, D.R., Hood, E.E., Peterson, D., Bailey, M., Bond, D., Kusnadi, A., Evangelista, R., Nikolov, Z., Wooge, C., Mehigh, R., Kappel, W., Register, J., and Howard, J.A. Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants. Molecular Breeding 1998, 4, 301–312.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Hood, E.E., Kusnadi, A., Nikolov, Z., Howard, J.A. (1999). Molecular Farming of Industrial Proteins from Transgenic Maize. In: Shahidi, F., Kolodziejczyk, P., Whitaker, J.R., Munguia, A.L., Fuller, G. (eds) Chemicals via Higher Plant Bioengineering. Advances in Experimental Medicine and Biology, vol 464. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4729-7_11
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4729-7_11
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7143-4
Online ISBN: 978-1-4615-4729-7
eBook Packages: Springer Book Archive