Abstract
Whole mammary glands from pubescent mice, primed for 9 days with estrogen and progesterone, cultured in the presence of the lactogenic hormone mix of insulin, aldosterone, hydrocortisone, and prolactin develop pregnancy-like lobuloalveolar structures and synthesize milk protein. Withdrawal of prolactin from the medium (with or without the steroids) results in involution of the gland in a manner comparable to that which occurs in vivo. Reincubation with the four-hormone mix plus epidermal growth factor results in a second round of development. Thus, whole organ culture provides an excellent model system to study systematically the hormonal and growth factor requirements that regulate the biochemical events that occur during these processes.
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Abbreviations
- (I):
-
insulin
- (E):
-
estradiol
- (P):
-
progesterone
- (H):
-
hydrocortisone
- (A):
-
aldosterone
- (PRL):
-
prolactin
- (EGF):
-
epidermal growth factor
- (PBS):
-
phosphate buffered saline
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Ginsburg, E., Vonderhaar, B.K. (2000). Whole Organ Culture of the Mouse Mammary Gland. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_14
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DOI: https://doi.org/10.1007/978-1-4615-4295-7_14
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