Abstract
The effects of changes in blood pH upon afferent, carotid sinus nerve (CSN) discharge are well documented: a decreased pHo increases firing rate (Gray, 1968; Biscoe, Purves & Sampson; 1970). The mechanisms by which this is brought about, however, remain largely unknown.A number of observations suggest that the type -I, or glomus, cell is the primary site of pH chemoreception (McClodcey, 1968; Ridderstrale & Hanson, 1984; Rigual, Inequez, Carreres & Gonzalez, 1985; Rigual, Lopez-Lopez & Gonzalez, 1991; Rigual, Gonzalez, Gonzalez & Fidone, 1986; Lopez-Lopez et al., 1989; Peers 1990). One of the fundamental questions concerning pH chemoreception is whether these cells respond primarily to a change of extracellular pH or to a secondary change of intracellular pH. We have shown that type-I cells possess at least three pHi-regulatory mechanism which serve, under conditions of constant pHo, to maintain pHi at a fairly constant level of 7.2–7.3 in physiological media (Buckler, Vaughan-Jones, Peers & Nye, 1991a). This has prompted us to determine the extent to which pHi in the type-I cell is influenced by external pH and to consider whether changes of pHi; are required for chemoreception of arterial pH.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Aickin,C.C.,1984,Direct measurement of intracellular pH and buffering power in smooth muscle cells of Guinea - pig vas deferens. J.Physiol., 349:571.
Aickin, C.C. & Thomas, R.C., 1977, Microelectrode measurement of the intracellular pH and buffering power of mouse soleus muscle fibres. J. Physiol., 267:571.
Aronson, P.S., 1985, Kinetic properties of the plasma membrane Na+-H+ exchanger. Ann. Rev. Physiol., 47:545.
Biscoe, T.J. & Duchen, M.R., 1990, Responses of type I cells dissociated from the rabbit carotid body to hypoxia. J. Physiol., 428:39.
Biscoe, T. J., Purves, M.J. & Sampson, S.R., 1970, The frequency of nerve impulses in single carotid body chemoreceptor afferent fibres recorded in vivo with intact circulation. J. Physiol., 208:121.
Buckler, K.J. & Vaughan-Jones, R.D., 1990, Application of a new pH-sensitive fluoroprobe (carboxyl-SNARF-I) for intracellular pH-measurement in small isolated cells. Pflugers Archive., 417:234.
Buckler, K. J., Vaughan-Jones, R. D., Peers, C. & Nye, P.C.G., 1991a, Intracellular pH and its regulation in isolated type I carotid-body cells of the neonatal rat. J. Physiol., 436:107.
Buckler, K.J., Vaughan-Jones, R.D., Peers, C., Lagadic-Gossmann, D. & Nye, P.C.G., 1991b, Effects of extracellular pH, pCO2 and HCO3 - on intracellular pH in isolated type I cells of the neonatal rat carotid body. J. Physiol., 444:703.
Buckler, K.J. & Vaughan-Jones, R.D., 1992, Effects of acidic stimuli on intracellular calcium in rat neonatal carotid body glomus cells. (Manuscript in preparation).
Ellis, D. & Thomas, R.C., 1976, Microelectrode measurement of the intracellular pH of mammalian heart cells. Nature, 262:224.
Gray, B.A., 1968, Responses of the perfused carotid body to changes in pH and pCO2. Resp. Physiol., 4:229.
Hornbein, T.F. & Roos, A., 1963, Specificity of H+ ion concentration as a carotid chemoreceptor stimulus. J. Appl. Physiol., 18:580.
Hornbein,T.F., 1968, The relation between stimulus to chemoreceptors and their response. In “Arterial chemoreceptors,” R.W. Torrance, Ed., Blackwell, Oxford.
Lopez-Lopez, J., Gonzalez, C., Urena, J. & Lopez-Barneo, J., 1989, Low PO2 selectivity inhibiytis K channel activity in chemoreceptor cells of the mammalian carotid body. J. Ge. Physiol., 93:1001–1015.
McCloskey, D.I., 1968, In:“Arterial chemoreceptors,” R.W. Torrance, Ed., Blackwell, Oxford: pp 279–296.
Peers, C., 1990, Selective effect of lowered extracellular pH on Ca2+-dependent K+ currents in type I cells isolated from the neonatal rat carotid body. J. Physiol., 422:381.
Peers, C. & Green, F.K., 1991, Inhibition of Ca2+-activated K+ currents by intracellular acidosis in isolated type-I cells of the neonatal rat carotid body. J. Physiol., 437:589.
Peers, C. & O’ Donnell, J., 1990, Potassium currents recorded in type-I carotid body cells from the neonatal rat and their modulation by chemoexcitatory agents. Brain Res., 522:259.
Ridderstrale, Y. & Hanson, M A., 1984, Histochemical localization of carbonic anhydrase in the cat carotid body. Ann. N. Y. Acad. Sci., 429:398.
Rigual, R., Gonzalez, E., Gonzalez, C. & Fidone,S., 1986, Synthesis and release of catecholamines by the cat carotid body in vitro: effects of hypoxic stimulation. Brain Res., 374:101.
Rigual, R., Iniguez, C., Carreres, J. & Gonzalez, C., 1985, Carbonic anhydrase in the carotid body and the carotid sinus nerve. Histochem., 82:577.
Rigual, R., Lopez-Lopez, J.R. & Gonzalez, C., 1991, Release of dopamine and chemoreceptor discharge induced by low pH and high pCO2 stimulation of the cat carotid body. J. Physiol., 433:519.
Rocher, A., Obeso, A., Gonzalez, C. & Herreros, B., 1991, Ionic mechanisms for the transduction of acidic stimuli in rabbit carotid body glomus cells. J. Physiol., 433:533.
Stea, A. & Nurse, C.A., 1989, Chloride channels in cultured glomus cells of the rat carotid body. Am. J. Physiol., 257:C174.
Tolkovsky, A.M. & Richards, C.D., 1987, Na+/H+ Exchange is the major mechanism of pH regulation in cultured sympathetic neurones: measurements in single cell bodies and neurites using a fluorescent pH indicator. Neurosci., 22:1093.
Vaughan-Jones, R.D., 1986, An investigation of chloride-bicarbonate exchange in the sheep cardiac Purkinje fibre. J. Physiol., 379:377.
Vaughan-Jones, RD. & Wu, Mei-Lin., 1990, Extracellular H+ inactivation of Na+ -H+ exchange in the sheep cardiac Purkinje fibre. J. Physiol., 428:441.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media New York
About this chapter
Cite this chapter
Buckler, K.J., Vaughan-Jones, R.D., Peers, C., Lagadic-Gossmann, D., Nye, P.C.G. (1993). The Modulation of Intracellular pH in Carotid Body Glomus Cells by Extracellular pH and pCO2 . In: Data, P.G., Acker, H., Lahiri, S. (eds) Neurobiology and Cell Physiology of Chemoreception. Advances in Experimental Medicine and Biology, vol 337. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2966-8_15
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2966-8_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6290-6
Online ISBN: 978-1-4615-2966-8
eBook Packages: Springer Book Archive