Abstract
Recently patch clamp techniques or optical fluorometric techniques have been applied to freshly dissociated or cultured carotid body glomus cells to understand chemosensory mechanisms of the carotid body. Results were not consistent. The inconsistency of the available data may result from various reasons. Firstly, the studied cells might have been heterogeneous due to the difficulty of differentiating between dissociated glomus cells and sheath cells. Secondly, culture conditions may alter characteristics of the glomus cells. Thirdly, differences in the age and species used to generate the available data make the interpretation more difficult. The purpose of this study is to standardise our culture techniques to overcome these difficulties.
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References
Banker, G. &Goslin, K. (1991). Culturing nerve cells. Cambridge, MA: The MIT press
Bottenstein, J.E. &Sato, G.H. (1979). Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc. Natl. Acad. Sci. U.S.A. 76:514–517.
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© 1994 Springer Science+Business Media New York
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Shirahata, M., Schofield, B., Chin, B.Y., Guilarte, T.R. (1994). Culturing Carotid Body Cells of Adult Cats. In: O’Regan, R.G., Nolan, P., McQueen, D.S., Paterson, D.J. (eds) Arterial Chemoreceptors. Advances in Experimental Medicine and Biology, vol 360. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2572-1_19
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DOI: https://doi.org/10.1007/978-1-4615-2572-1_19
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