Abstract
Dendritic cells (DC) represent a subpopulation of leukocytes of bone marrow (BM) origin, involved in crucial immunological reactions. DC play a fundamental role in the primary immune response by stimulating quiescent T cells. In this study we describe an in vitro culture system to raise DC from unfractionated bone marrow (BM) cells of LEWIS rats in the presence of low doses of mouse recombinant GM-CSF, that was successfully used in previous work to culture mouse DC1,2,3.
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© 1995 Springer Science+Business Media New York
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Mehlig, M., Scheicher, C., Dienes, HP., Reske, K. (1995). Development of Rat DC by in Vitro Culture of Bone Marrow Cells. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_14
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DOI: https://doi.org/10.1007/978-1-4615-1971-3_14
Publisher Name: Springer, Boston, MA
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