Abstract
In the present study HIV-1 quasispecies were analysed in Langerhans cells (LC) derived from eight split-thickness skin patches (Bl-8) obtained from clinically normal skin taken soon after autopsy from an AIDS patient (coded RI). RI was an Italian intravenous drug user who had been HIV-1 Ab positive from 1986. Fifteen days before death, the blood CD4+ and CD8+ T-cell counts were 9 and 165/γl respectively, while the serum p24 titre was 73pg/ml. Purification of LC from the skin samples was carried out as previously described1. The LC preparations were devoid of T cells, as demonstrated by reverse transcription PCR analysis for the T-cell receptor ß-chain gene constant region2. Immunostaining of cytospin preparations2 failed to reveal any CD14+ cells (monocytes/macrophage lineage) in purified LC fraction. Patches Bl, B2, B3, B4 were disposed in a clockwise manner and derived from one thigh, while B5, B6, B7, B8 were similarly related yet from the other.
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Sala, M. et al. (1995). Discontinuous Distribution of HIV-1 Quasispecies in Epidermal Langerhans Cells of an AIDS Patient and Evidence for Double Infection. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_108
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DOI: https://doi.org/10.1007/978-1-4615-1971-3_108
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