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Methods for Assessing Proteolysis in Cheese During Maturation

  • Chapter
Chemistry of Structure-Function Relationships in Cheese

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 367))

Abstract

Proteolysis is the principal and most complex biochemical event occurring during the maturation of the majority of ripened cheese varieties. The extent of proteolysis varies from very limited (e.g., Mozzarella) to very extensive (e.g., blue-mould varieties). The products of proteolysis vary from large polypeptides, comparable in size with intact caseins, through a range of intermediate-sized and small peptides to free amino acids and their degradation products. Proteolysis in cheese involves a complex and dynamic series of events and has important implications for the development of the correct flavour and texture characteristic of the variety. Owing to its complexity and importance to the biochemistry of cheese ripening, proteolysis in cheese has been the subject of much study and a wide range of analytical techniques have been developed to assess its extent and nature.

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References

  • Abd-El-Salam, M.H., and El-Shibiny, S., 1972, Changes in proteins of Domiati cheese during pickling, J. Dairy Res. 39:219.

    Article  CAS  Google Scholar 

  • Abdel Baky, A.A., Abo Ella, W.M., Aly, M.E., and Fox, P.E., 1987, Improving the quality of Ras-type cheese made from recombined milk containing high levels of total solids, Food Chem. 26:175.

    Article  Google Scholar 

  • Abo El-Ella, W.M., Abdel Baky, A.A., Aly, M.E., and Fox, P.E., 1988, Effect of ripening temperatures on proteolysis and lipolysis in the outer and inner regions of Ras-type cheese made by various salting methods, Food Chem 28:1.

    Article  Google Scholar 

  • Addeo, F., Chianese, L., Di Luccia, A., Petrilli, P., Mauriello, R., mid Anelli, G., 1983, Identification of bovine casein variants by gel isoelectric focusing, Milchwissenschaft 38:586.

    CAS  Google Scholar 

  • Addeo, F., Moio, L., Chianese, L., Stingo, C, and Di Luccia, A., 1990a, Improved procedure for detecting bovine and ovine milk mixtures in cheese by isoelectric focusing of para-κ-casein,Milchwissenschaft 45:221.

    CAS  Google Scholar 

  • Addeo, F., Moio, L., Chianese, L., Stingo, C, Resmini, E., Berner, I., Krause, D., Di Luccia, A., and Bocca, A., 1990b, Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine cheesee by gel isoelectric focusing of 72-caseins, Milchwissenschafl 45:708.

    CAS  Google Scholar 

  • Addeo, F., Chianese, L., Salzano, A., Sacchi, R., Cappuccio, U., Ferranti, P., and Malorni, A., 1992,Characterization of the 12% trichloroacetic acid-soluble oJigopeptides of Pannigiano-Reggianocheese, J. Dairy Res. 59:401.

    Article  CAS  Google Scholar 

  • Adler-Nissen, J., 1979, Determination of the degree of hydrolysis of food protein hydrolyzates by trinitrobenzene-sulphonic acid, J. Agric. Food Chem. 27:1256.

    Article  CAS  Google Scholar 

  • Ali, L.A.M.,and Mulder, H., 1961, Taste and flavour forming compounds in cheese. Die amino acid content of Edam cheese, Neth. Milk Dairy J. 15:377.

    CAS  Google Scholar 

  • Ali, M.M., Al-Dalihan, D.H., Abo-Elnaga I.G., and Zanco, A., 1980, Performance of single strain starter inthe manufacture of Cheddar cheese in Iraq, Pure Appl. Sciences 6:97, cited from Food Sci. Technol.Abstr ,1984,16:9P2064.Abstr , 1984, 16:9P2064.

    Google Scholar 

  • Amanlea, G.F., Skura, B.J. and Nakai, S., 1986, Culture effect on ripening characteristics and rheological behaviour of Cheddar cheese, J. Food Sci. 51:912.

    Article  Google Scholar 

  • Amigo, L., Ramos, M., Calhau, L. and Barbosa, M., 1992, Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determination of cow’s and goat’s milk in Serra da Estrela cheeses,Lait 72: 95.

    Article  CAS  Google Scholar 

  • Andrews, A.T., 1983, Proteinases in normal bovine milk and their action on caseins, J. Dairy Res. 50:45.

    Article  CAS  Google Scholar 

  • Ardo, Y. and Gripon, J.-C., 1991, Chromatographie methods used to measure proteolysis in cheese, Bulletin 261, p 29, International Dairy Federation, Brussels.

    Google Scholar 

  • Ardo, Y. and Meisel, H., 1991, Methods for direct measurement of peptide bond cleavage in cheese. Bulletin 261, p 10, International Dairy Federation, Brussels.

    Google Scholar 

  • Ardo, Y. and Pettersson, H.-E., 1988, Accelerated cheese ripening with heat treated cells of Lactobacillus helveticus and a commercial proteolytic enzyme, J. Dairy Res. 55:239.

    Article  CAS  Google Scholar 

  • Ashworth, V.S., 1966, Determination of protein in dairy products by dye-binding, J. Dairy Sci. 49:133.

    Article  CAS  Google Scholar 

  • Aston, J.W. and Creamer, L.K., 1986, Contribution of the components of the water-soluble fraction to the flavour of Cheddar cheese, N.Z. J. Dairy Sci. Technol. 21:229.

    CAS  Google Scholar 

  • Aston, J.W. and Dulley, J.R., 1982, Cheddar cheese flavour, Aust. J. Dairy Technol. 37: 59.

    CAS  Google Scholar 

  • Aston, J.W., Durward, I.G. and Dulley, J.R., 1983a, Proteolysis and flavour development in Cheddar cheese, Aust. J. Dairy Technol. 38:55.

    CAS  Google Scholar 

  • Aston, J.W., Grieve, P.A., Durward, I.G. and Dulley, J.R., 1983b, Proteolysis and flavour development in Cheddar cheeses subjected to accelerated ripening treatments, Aust. J. Dairy Technol. 38:59.

    CAS  Google Scholar 

  • Attia, I.A. and Gooda, E., 1987, The chemical composition in relation to the flora of market Ras cheese, Egypt. J. Dairy Sci. 15:135, cited from Food Sci. Technol , Abstr ,1987, 19:11P82.

    CAS  Google Scholar 

  • Attia, I.A., Gooda, E., and Salem, S. A., 1990, Gel filtration of soluble nitrogeneous compounds and free amino acids in hydrolysed lactose Kachkoval cheese during ripening, Alexandria Sci. Exchange 11:93, cited from Food Sci. Technol. Abstr, 1991, 23:7P71.

    Google Scholar 

  • Bachmann, M., and Schaub, W., 1974, Measuring casein-breakdown in cheese by gel filtration, Proc. 19th Intern. Dairy Congr., New Delhi, 1E:271.

    Google Scholar 

  • Barlow, I.E., Lloyd, G.T., and Ramshaw, E.H., 1986, The measurement of proteolysis in Cheddar cheese: a comparison of trinitrobenzene sulphonic acid procedures, Aust. J. Dairy Technol. 41:79.

    CAS  Google Scholar 

  • Barrefors, P., Ekstrand, B., Fagerstam, L., Larson-Raznikiewicz, M., Scharr, J., and Steffner, P., 1985, Fast protein liquid cliromatography (FPLC) of bovine caseins, Milchwissenschafl 40:257.

    CAS  Google Scholar 

  • Barthel, C, Sandberg, E., and Haglund, E., 1928, Une nouvelle méthiode pour l’étude du procédé de la maturation des fromages. Communication préliminaire, Lait 8: 285.

    Article  CAS  Google Scholar 

  • Basch, J.J., Farrell, H.M.,Jr., Walsh, R.A., Konstance, R.P., and Kumosinski, T.F., 1989, Development of a quantitative model for enzyme-catalyzed, time-dependent changes in protein composition of Cheddar cheese during storage, J. Dairy Sci. 72:591.

    Article  CAS  Google Scholar 

  • Beeby, R., 1980, Use of fluorescamine at pH 6.0 to follow the action of chymosin on κ-casein and to estimate this protein in milk, N.Z. J. Dairy Sci. Technol ,15:99.

    CAS  Google Scholar 

  • Bertacco G., Boschella, O., and Lercker, G., 1992, Gas chromatographic determination of free amino acids in cheese, Milchwissenschaft 47:348.

    CAS  Google Scholar 

  • Bhomik, T., Riesterer, R., van Boetal, M.A.J.S., and Marth, E.H., 1990, Characteristics of low-fat Cheddar cheese made with added Micrococcus or Pediococcus species, Milchwissenschafl 45:230.

    Google Scholar 

  • Bican, P., and Blanc, B., 1982, Milk protein analysis, a high performance chromatography study, Milchwissenschaft 37: 592.

    CAS  Google Scholar 

  • Bican, P., and Spahni, A., 1991, Low molecular mass nitrogen components in ripening cheese, Lebensm .-Wiss . Technoi 24:315.

    CAS  Google Scholar 

  • Biede, S.L., and Hammond, E.G., 1979, Swiss cheese. 1. Chemical analysis, J. Dairy Sci. 62:227.

    Article  CAS  Google Scholar 

  • Blakesley, R.W., and Boezi, J.A., 1977, A new staining technique for proteins in polyacrylaande gels using Coomassie Brilliant Blue G250, Anal. Biochem. 82:580.

    Article  CAS  Google Scholar 

  • Bradford, M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72:248.

    Article  CAS  Google Scholar 

  • Breen, E.D., 1992, “Fractionation of Water-soluble and Water-insoluble Cheese Nitrogen,“ M.Sc. Thesis, National University of Ireland, Cork.

    Google Scholar 

  • Broome, M.C., Krause, D.A., and Hickey, M.W., 1990, The use of non-starter lactobacilli in Cheddar cheese manufacture, Aust. J. Dairy Technoi. 45:67.

    Google Scholar 

  • Broome, M.C., Krause, D.A., and Hickey, M.W., 1991, The use of proteinase negative starter and lactobacilli in Cheddar cheese manufacture, Aust. J. Dairy Technoi. 46:6.

    Google Scholar 

  • Bullock D.H., and Irvine, O.R., 1956, A cliromatographic study of Cheddar cheese ripening, J. Dairy Sci. 39:1229.

    Article  CAS  Google Scholar 

  • Buruiana, L.M., and Farag, S.I., 1983, The variation of amino acids in Telemea cheese during pickling,Egypt.J. Dairy Sci. 11:53.

    CAS  Google Scholar 

  • Buruiana, L.M., and Zeidan, A.N., 1982, Variation of free amino acids in Kashkawal ‘Dalia’ cheese during ripening, Egypt. J. Dairy Sci. 10:209.

    CAS  Google Scholar 

  • Bütikofer, U., Fuchs, D., Murni, D., and Bosset, J.O., 1990, Beitrag zur Bestimmung biogener Aniine in Käse. Vergleich einer verbesserten HPLC-mit einer IC-methode und miwendung bei verschiedenen Kasesorten, Mitt. Gebiete Lebensm. Hyg. 81:120.

    Google Scholar 

  • Bütikofer, U., Fuchs, D., Bosset, J.O., and Gmur, W., 1991, Automated HPLC-amiiio acid determination by precolumn derivitization with OPA and FMOC and comparison with classical ion-exchange chromatography, Chromatogr . 31:441.

    Article  Google Scholar 

  • Bütikofer, U., Ruegg, M., and Ardo, Y., 1993, Determination of nitrogen fractions in cheese: evaluation of a collaborative study, Lebensm . Wiss. u. Technoi. 26: 271.

    Google Scholar 

  • Bynum, D.G., and Barbano, D.M., 1985, Whole milk reverse osmosis retentate for Cheddar cheese manufacture: chemical changes during ripening, J. Dairy Sci. 68:1.

    Article  CAS  Google Scholar 

  • Chakravotry, S.C., Srinivasan, R.A., Babbar, I.J., Dudani, A.T., Burde, S.D., and Iya, K.K., 1966, Comparative study of proteolysis during ripening of Cheddar cheese by bacterial milk clotting enzymes and rennet extract, Proc. 17th Intern. Dairy Congr., Munich, D:I87.

    Google Scholar 

  • Champion, H.M., and Stanley D.W., 1982, HPLC separation of bitter peptides from Cheddar cheese, Can. bist. Food Sci. Technoi . J. 15:283.

    CAS  Google Scholar 

  • Christensen, T.M.I.E., Been, A.-M., and Werner, H., 1991, Methods for crude fractionation (extraction and precipitation) of nitrogen components in cheese, Bulletin 261, p 4, International Dairy Federation,Brussels

    Google Scholar 

  • Christensen, T.M.I.E., Kristiansen, K.R., and Madsen, J.S., 1989, Proteolysis in cheese investigated by high performance liquid chromatography, J. Dairy Res. 56:823.

    Article  Google Scholar 

  • Church, F.C., Swaisgood, H.E., Porter, D.H., and Catignani, G.L., 1983, Spectrophotometric assay using <?-phüialdialdehyde for determination of proteolysis in milk and isolated milk proteins, J. Dairy Sci.66:1219.

    Article  CAS  Google Scholar 

  • Cifuentes, A., de Frutos, M., and Diez-Masa, J. C, 1993, Analysis of whey proteins by capillary electrophoresis using buffer-containing polymeric additives, J. Dairy Sci. 76:1870

    Article  CAS  Google Scholar 

  • Clegg, K.M., Lee, Y.K., and McGilligan, J.F., 1982, Technical note: trinitrobenzene-sulphonic acid and ninhydrin reagents for the assessment of protein degradation in cheese samples, J. Food Technoi. 17:

    Google Scholar 

  • Clemens, W., 1954, Papierchromatographische Untersuchungen an Käse, Milchwissenschaft 9:195.

    CAS  Google Scholar 

  • Cliffe, A.J., and Law, B.A., 1990, Peptide composition of enzyme-treated Cheddar cheese slurries, determined by reverse phase liquid chromatography, Food Chem. 36:73.

    Article  CAS  Google Scholar 

  • Cliffe, A.J., and Law, B.A., 1991, A time course study of peptide production in accelerated-ripened Cheddar cheese using reverse phase high performance liquid chromatography, Food Biotechnol. 5:1.

    Article  CAS  Google Scholar 

  • Cliffe, A.J., Marks, J.D., and Mulholland, F., 1993, Isolation and characterization of non-volatile flavours from cheese: peptide profile of flavour fractions from Cheddar cheese, determined by reverse phase high-performance liquid chromatography, Intern. Dairy J. 3:379.

    Article  CAS  Google Scholar 

  • Cliffe, A.J., Revell, D., and Law, B.A., 1989, A method for the reverse phase high performance liquid chromatography of peptides from Cheddar cheese, Food Chem. 34:147.

    Article  CAS  Google Scholar 

  • Conti, A., Napolitano, L., Cantisani, S.M., Davoli, R., and Dall-Olio, S., 1988, Bovine beta-lactoglobulin H -isolation by preparative isoelectric-focusing in immobilized pH gradients and preliminary characterization, J. Biochem. Biophys. Methods 16:205.

    Article  CAS  Google Scholar 

  • Creamer, L.K., 1975, ß-Casein degradation in Gouda and Cheddar cheese, J. Dairy Sci. 58: 287.

    Article  CAS  Google Scholar 

  • Creamer, L.K., 1991, Electrophoresis of cheese, Bulletin 261, p 14, Internernational Dairy Federation, Brussels.

    Google Scholar 

  • Creamer, L.K., and Richardson, B.C., 1974, Identification of the primary degradation product of αs1-casein in Cheddar cheese, N.Z. J. DairySciTeehnol. 9:9.

    CAS  Google Scholar 

  • Creamer, L.K., Aston, A., and Knighton D., 1988, Some differences between Cheddar cheeses made using calf rennet and a microbial coagulant (Rennilase 464), N.Z. J. DairySciTeehnol 23:185.

    CAS  Google Scholar 

  • Creamer, L.K., Lawrence, R.C., and Gilles, J., 1985, Effect of acidification of cheese milk on the resultant Cheddar cheese, N.Z. J. DairySciTeehnol. 20:185.

    CAS  Google Scholar 

  • Dahlberg, A.C., and Kosikowsky ,F.V., 1947, The flavour, volatile acidity and soluble protein of Cheddar and oüier cheese, J. Dairy Sci. 30:165.

    Article  CAS  Google Scholar 

  • Davis, J.G., 1965, in : “Cheese,“ Vol. 1, Basic Technology, p 308, J. &A. Churchill, Ltd., London.

    Google Scholar 

  • de Jong, L., 1975, A quantitative electrophoretic method of studying cheese ripening, Neth. Milk Dairy J. 29:162.

    Google Scholar 

  • Desmazeaud, M.J., Gripon, J.-C., La Bars, D. and Bergere, J.L., 1976, Etude du role des micro-organisms et des enzymes au cours de la maturation des formages. III. Influence des micro-organisms, Lait 56:379.

    Article  CAS  Google Scholar 

  • Desyatnikova, O.I., and Alexaev, N.S., 1979, Amino acids and protein value of ewes’ milk cheese (Bryndza) proteins, Izvestiya vysshikh Uchebnykh Zavedeii Pishchevaya Tekhnologiya 5:29, cited from Food Sci. Teehnol. Abstr. ,1981, 13:2P309.

    Google Scholar 

  • Doi, E., Shibata, D., and Matoba, T., 1981, Modified colorimetric ninhydrin methods for peptidase assay, Anal. Biochem. 118:173.

    Article  CAS  Google Scholar 

  • Edwards, J., and Kosikowski, F.V., 1983, Bitter compounds from Cheddar cheese, J. Daiiy Sci. 66:727.

    Article  CAS  Google Scholar 

  • El Soda, M., Desmazeaud, M.J., Aboudonia, S., and Kamal, N., 1981, Acceleration of cheese ripening by the addition of whole cells or cell free extracts from Lactobacillus casei to the cheese curd Milchwissenschaft 36:140.

    Google Scholar 

  • Farkye, N.Y., and Fox, P.F., 1991, Preliminary study on the contribution of plasmin to proteolysis in Cheddar cheese: cheese containing plasmin inhibitor, 6-aminohexanoic acid, J. Agric. Food Chem.39:786.

    Article  CAS  Google Scholar 

  • Farkye, N.Y., and Fox, P.F., 1992, Contribution of plasmin to Cheddar cheese ripening: effect of added plasmin, J. Dairy Res. 59:209.

    Article  CAS  Google Scholar 

  • Farkye, N.Y., Fox, P.F., Filzgerald, G.F., and Daly, C, 1990, Proteolysis and flavour development in Cheddar cheese made exclusively with single strain protcinase-positive or proteinase-negative starters, J. Dairy Sci. 73:874.

    Article  CAS  Google Scholar 

  • Fernandez del Pozo, B., Gaya, P., Medina, M., Rodriguez-Martin, M.A., and Nunez, M., 1988, Changes in chemical and rheological characteristics of La Serena ewes’ milk cheese during ripening, J. DairyRes. 55:457.

    Article  Google Scholar 

  • Fields, R., 1971, The measurement of amino groups in proteins and peptides, Biochem. J. 124:581.

    CAS  Google Scholar 

  • Folkertsma, B., and Fox, P.F., 1992, Use of Cd-ninhydrin reagent to assess proteolysis in cheese during ripening, J. Dairy Res. 59:217.

    Article  CAS  Google Scholar 

  • Foster, P.M.D., and Green, M.L., 1974, A quantitative gel filtration method for analysis of the proteinaceous fraction of Cheddar cheese, J. Dairy Res. 41:259.

    Article  CAS  Google Scholar 

  • Fox, P.F., 1989, Proteolysis in cheese during manufacturing and ripening, J. Dairy Sci. 72:1379.

    Article  CAS  Google Scholar 

  • Friedman, M., Pang, J., and Smith, G.A., 1984, Ninhydrin-reactive lysine in food proteins, J. Food Sci. 49:10.

    Article  Google Scholar 

  • Frister, H., Meisel, H., and Schlimme, E, 1989, Photometric measurement of proteolysis in semi-hard cheese using the modified OPA-method, Kierler Milchwirtschaftliehe Forschungsberichte 41:237.

    CAS  Google Scholar 

  • Furtado, M.M., and Partridge, J.A., 1988, Characterization of nitrogen fractions during ripening of a soft cheese made from ultrafiltration retentate, J. Dairy Sci. 71:2877.

    Article  CAS  Google Scholar 

  • Gonashvily, S., 1966, Protein substances in cheese, Proc. 17th Intern. Dairy Congr., Munich, D:289.

    Google Scholar 

  • Gonzalez de Llano, D., Polo, C., and Ramos, M., 1991, Production, isolation and identification of low molecular mass peptides from Blue cheese by high performance liquid chromatography, J. Dairy? Res.58:363.

    Article  Google Scholar 

  • Gonzalez de Llano, D., Ramos, M.,and Polo, C, 1987, Gel filtration and high performance liquid Chromatographie analysis of phosphotuiigstic acid soluble peptides from Blue cheeses, Chromatog.23:764

    Article  Google Scholar 

  • Green, M.L., and Foster, P.M.D., 1974, Comparison of the rates of proteolysis during ripening of Cheddar cheeses made with calf rennet and swine pepsin as coagulants, J. Dairy Res. 41: 269.

    Article  CAS  Google Scholar 

  • Green, M.L., and Stackpoole, A., 1975, The preparation and assessment of a suitable Mucor pusillus Lindt proteinase-swine pepsin mixture for Cheddar cheese making, J. Dairy Res. 42:297.

    Article  CAS  Google Scholar 

  • Gripon, J.C., Desmazeaud, M.J., Le Bars, D., and Bergere, J.-L., 1975, Etude du role des micro-organismes etdes enzymes au cours de la maturation des fromages. II. Influence de la presure commerciale, Lait 55:502.

    Article  CAS  Google Scholar 

  • Gripon, J.C., Desmazeaud, M.J., Le Bars, D., and Bergere, J.-L., 1977, Role of proteolytic enzymes ofStreptococcus lactis, Penicillium roqueforti and Penicillium caseicolum during cheese ripening J. Dairy Sci. 60:1532.

    Article  CAS  Google Scholar 

  • Guigoz, Y., and Solms, J., 1974, Isolation of a bitter testing pepticle from “Alpkäse,“ a Swiss mountaincheese, Lebensmittelwiss. Technol. 7:356.

    CAS  Google Scholar 

  • Guinee, T.P., Wilkinson, M.G., Mulholland, E.O., and Fox, P.F., 1991, Influence of ripening temperature, added commercial enzyme preparations and attenuated mutant (Lac) Lactococcus lactis starter on the proteolysis and maturation of Cheddar cheese, Irish J. FoodSciTechnol. 15:27.

    Google Scholar 

  • Gupta, S.K., Whitney, R.M., and Tuckey, S.L., 1974, Brine soluble protein of Cheddar and Gouda cheese, J. Dairy Sci. 57: 540.

    Article  CAS  Google Scholar 

  • Haasnoot, W., Venema, D.P., and Elenbaas, H.L., 1986, Determination of cow milk in the milk and cheese of ewes and goats by fast protein liquid chromatography, Milchwissenschafl 41:642.

    CAS  Google Scholar 

  • Haasnoot, W., Stouter, P., and Venema, D.P., 1989, High performance liquid chromatography determination of the extent of proteolysis in Gouda cheese. J. Chromatogr. 483:319.

    Article  CAS  Google Scholar 

  • Habeeb, A.F.S., 1966, Determination of free amino groups in proteins by trinitrobenzene-sulfonic acid, Anal. Biochem. 14:328.

    Article  CAS  Google Scholar 

  • Harwalkar, V.R., 1967, Comparative study of bitter flavor fraction obtained from non-bitter and bitter Cheddar cheese, J. Dairy Sci. 50:956.

    Google Scholar 

  • Harwalkar, V.R., and Elliot, J.A., 1965, Isolation and partial purification of bitter components from Cheddar cheese, J. Dairy Sci. 48:784. .

    Article  Google Scholar 

  • Harwalkar, V.R., and Elliott, J.A., 1971, Isolation of bitter and astringent fractions from Cheddar cheese, J. Dairy Sci. 54: 8.

    Article  CAS  Google Scholar 

  • Hickey, M.W., van Leeuwen, H., Hillier, A.J., and Jago, G.R., 1983, Amino acid accumulation in Cheddar cheese manufactured from normal and ultrafiltered milk, Aust. J. Dairy Technol. 38:110.

    CAS  Google Scholar 

  • Hiniz, P.C., Slattery, W.L., and Harper, W.J., 1956, A survey of various free amino and fatty acids in domestic Swiss cheese, J. Dairy Sci. 39:235.

    Article  Google Scholar 

  • Huber, L., and Klostermeyer, II., 1974, Isolierung und Identifizierung eines Bitterstoffes aus Butterkäse, Milchwissenschaft 29: 449.

    CAS  Google Scholar 

  • Hull, M.E., 1947, Studies on milk proteins. II. Colorimetric determination of die partial hydrolysis of die proteins in milk, J. Dairy Sci. 30:881.

    Article  CAS  Google Scholar 

  • Humbert, G., Guingamp, M.-F., Kouomegne, R., and Linden, G., 1990, Measurement of proteolysis in milk and cheese using trinitrobenzene sulphonic acid and a new dissolving reagent, J. Dairy Res. 57:143.

    Article  CAS  Google Scholar 

  • Hwang, J.H., Huh, J.W., and Yu, J.H., 1987, Flavour changes during ripening of domestic Cheddar cheese. Changes in free amino acids, ripening degree and gross composition, Korean J. Dairy Sci. 9:101, cited from FoodSciTechnol. Abstr ,1988, 20:4P98.

    Google Scholar 

  • Inikhov, G.S., 1951, “Chemical Analysis of Milk Products,“ Part II, Food Industry Publ., Moscow.

    Google Scholar 

  • International Dairy Federation, 1991, Chemical methods for evaluating proteolysis in cheese maturation, IDF Bulletin 261, International Dairy Federation, Brussels.

    Google Scholar 

  • Ismail, A.A., and Hansen, K., 1972, Accumulation of free amino acids during cheese ripening of some types of Danish cheese, Milchwissenschafl 27:556.

    CAS  Google Scholar 

  • Jarrett, W.D., Aston, J.W., and Dulley, J.R., 1982, A simple method for estimating free amino acids in Cheddar cheese, Aust. J. Dairy Technol. 37: 55.

    CAS  Google Scholar 

  • Kaminogawa, S., Yan, T.R., Azuma, N., and Yamauchi, K., 1986, Identification of low molecular weight peptides in Gouda-type cheese and evidence for the formation of these peptides from 23-N-terminal residues of αs1-casein by proteinases of Streptococcus cremoris H61, J. Food Sci. 51:1253.

    Article  CAS  Google Scholar 

  • Kieler, G., 1976, The ripening of Gouda cheese made under strict aseptic conditions. 1. Cheese with no other bacterial enzymes than those from a starter Streptococcus, Neth. Milk Dairy J. 30:254.

    Google Scholar 

  • Kosikowsky, F.V., 1951a, Paper chromatography of the free amino acids in American Cheddar cheese, J. Dairy Sci. 34:228.

    Article  CAS  Google Scholar 

  • Kosikowsky, F.V., 1951b, The liberation of the free amino acids in raw and pasteurized milk Cheddar cheese during ripening, J. Dairy Sci. 34:235.

    Article  CAS  Google Scholar 

  • Kosikowsky, F.V., and Dahlberg, A.C., 1954, A quantitative appraisal of the free amino acids in foreign type cheese, J. Dairy Sci. 37:167.

    Article  CAS  Google Scholar 

  • Kostyra, H., and Damicz, W., 1979, Comparison of effectiveness of extraction of peptides and the amino acids from Rokpol cheese using 3 solvent systems, Zes Naukowe. Akadem. Rolniczo Techniczo Technic. Olsztynie Technol. Zymosci. 15:91, cited from FoodSciTechnol. Abstr. ,1981, 13:6P1024.

    Google Scholar 

  • Kroger, M., and Weaver, J.C., 1979, Use of protein dye-binding values as indicators of the “chemical age” of conventionally made Cheddar cheese and hydrolyzed-lactose Cheddar cheese, J. Food Sci. 44:304.

    Article  CAS  Google Scholar 

  • Kuchroo, C.N., and Fox, P.F., 1982a, Soluble nitrogen in Cheddar cheese: Comparison of extraction procedures, Milchwissenschaft 37:331.

    CAS  Google Scholar 

  • Kuchroo, C.N., and Fox, P.F., 1982b, Fractionation of water-soluble nitrogen from Cheddar cheese: chemical methods, Milchwissenschafl 37:651.

    CAS  Google Scholar 

  • Kuchroo, C.N., and Fox, P.F., 1983a, Fractionation of the water-soluble nitrogen from Cheddar cheese: Chromatographie methods, Milchwissenschafl 38:76.

    CAS  Google Scholar 

  • Kuchroo, C.N., and Fox, P.F., 1983b, A fractionation scheme for the water-soluble nitrogen in Cheddar cheese, Milchwissenschaft 38:389.

    CAS  Google Scholar 

  • Kuchroo, C.N., Rahilly, J. and Fox, P.F., 1983, Assessment of proteolysis in cheese by reaction with trinitrobenzene sulphonic acid, Irish J. FoodSciTechnol. 7:129.

    CAS  Google Scholar 

  • Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227:680.

    Article  CAS  Google Scholar 

  • Laleye, L.C., Simard R.E., Grosselin, C, Lee, B.H., and Giroux, R.N., 1987, Assessment of Cheddar cheese quality by Chromatographie analysis of free amino acids and biogenic amines, J. Food Sci. 52:303.

    Article  CAS  Google Scholar 

  • Lau, K.Y., Barbano, D.M., and Rasmussen, R.R., 1990, Influence of pasteurization on fat and nitrogen recoveries and Cheddar cheese yield, J. Dairy Sci. 73:561.

    Article  Google Scholar 

  • Lau, K.Y., Barbano, D.M., and Rasmussen, R.R., 1991, Influence of pasteurization on protein breakdown in Cheddar cheese during aging, J. Dairy Sci. 74:727.

    Article  CAS  Google Scholar 

  • Lavanchy, P., and Buhlmann, C, 1983, Normal values of some important parameters of metabolism of cheeses made in Switzerland, Schweiz. Milchwirtschaft. Forsch. 12:3, cited from Food Sci. Technol.Abstr. ,1984, I6:3P623.Abstr. ,1984, I6:3P623.

    Google Scholar 

  • Law, J., Fitzgerald, G.F., Daly, C, Fox, P.F., and Farkye, N.Y., 1992, Proteolysis and flavour development in Cheddar cheese made with the single starter strains Lactococcus lactis ssp. lactis UC 317 or Lactococcus lactis ssp. cremoris HP, J. Dairy Sci. 75:1173.

    Article  CAS  Google Scholar 

  • Law, J., Fitzgerald, G.F., Uniacke-Lowe, T., Daly, C, and Fox, P.F., 1993, The contribution of lactococcal starter proteinases to proteolysis in Cheddar cheese, J. Dairy Sci. 76:2455.

    Article  CAS  Google Scholar 

  • Le Bars, D., and Gripon, J.-C., 1989, Specificity of plasmin towards bovine αs2-casein, J. Dairy Res. 56:817.

    Article  Google Scholar 

  • Ledford, R.A., O’Sullivan, A.C., and Nath, K.R., 1966, Residual casein fractions in ripened cheese determined by polyacryIainide-gel electrophoresis, J. Daiiy Sci. 49:1098.

    Article  CAS  Google Scholar 

  • Lemieux, L., and Amiot, J., 1989, Application of reversed-phase high performance liquid chromatography to the separation of peptides from phosphorylated and dephosphorylated casein hydrolysates,J. Chromatogr. 473:189.

    Article  CAS  Google Scholar 

  • Lemieux, L., and Simard, RE., 1991, Bitter flavour in dairy products. I. A review of the factors likely to influence its development, mainly in cheese manufacture, Lait 71:599.

    Article  CAS  Google Scholar 

  • Lemieux, L., and Simard, R.E., 1992, Bitter flavour in dairy products. II. A review of bitter peptides from caseins: their formation, isolation and identification, structure masking and inhibition, Lait 72:335.

    Article  CAS  Google Scholar 

  • Lemieux L., Puchades, R., and Simard, R.E., 1989, Size-exclusion HPLC separation of bitter and astringent fractions from Cheddar cheese made with added Lactobacillus strains to accelerate ripening, 7. Food Sci. 54:1234.

    Article  CAS  Google Scholar 

  • Lemieux, L., Puchades, R., and Simard, R.E., 1990, Free amino acids in Cheddar cheese: comparison of quantitation methods, J. Food Sci. 55:1552.

    Article  CAS  Google Scholar 

  • Lindqvist, B., 1962, Preparative separation of high molecular weight peptides of cheese, Proc. 16th Intern. Dairy Congr., Copenhagen, B:673.

    Google Scholar 

  • Lindqvist, B., and Storgards, T., 1959, Changes in casein during cheese ripening, Proc. 15th Intern. Dairy Congr., London, 2:679.

    Google Scholar 

  • Lindqvist, B., Storgards, T., and Goransson, M.-B., 1953a, Electrophoresis in paper as a means of studying the ripening process in cheese, Proc. 13th Intern. Dairy Congr., The Hague, 3:1261.

    Google Scholar 

  • Lindqvist, B., Lindberg, I., and Molin, H., 1963, Investigation of a 37 year old cheese, Milchwissenschaf ì 18:12.

    CAS  Google Scholar 

  • Lorenz, K., 1974, A new photometric method for the determination of amino acids in biological fluids using p-benzoquinone, Z. Analytische Chemie 269:182.

    Article  Google Scholar 

  • Lucey, J.A., Gorry, C., and Fox, P.F., 1993, Changes in the acid-base buffering curves during the ripening of Emmental cheese, Milchwissenschaf ì 48:183.

    CAS  Google Scholar 

  • Mabbitt, L.A., 1955, Quantitative estimation of the amino acids in Cheddar cheese and their importance in flavour, J. Dairy Res. 22:224.

    Article  CAS  Google Scholar 

  • Madkor, S.A., Fox, P.F., Shalabi, S.I., and Metwally, N.H., 1987, Studies on the ripening of Stilton cheese: Proteolysis, Food Chem. 25:13.

    Article  CAS  Google Scholar 

  • Mahmoud, S.A.Z., Khader, A.E., Salem, O.M., Moussa, A.M., and Kebary, K.M., 1983, Studies on Blue veined cheese. II. Amino and fatty acids, Minufiya J. Agric. Res. 6:207, cited from Food Sci.Technol Abstr. ,1984, l6:6Pl446.

    Google Scholar 

  • March, J.F., 1975, A modified technique for the quantitative analysis of amino acids by gas chromatography using heptafluorobutyric-fl-propyl derivatives, Anal Biochem. 69:420.

    Article  CAS  Google Scholar 

  • Marcos, A., and Esteban, M.A., 1993, Iberian cheeses, in, “Cheese: Chemistry, Physics and Microbiology,“ 2nded., Vol. 2, p 173, P.F. Fox, ed., Chapman &Hall, London.

    Google Scholar 

  • Marshall, T., Gilliland, D., and Williams, K.M., 1988, Sodium dodecylsulphateJ.polyacrylaande gel electrophoresis of milk proteins: application to cheese, Biochem. Soc. Trans. 16:165.

    CAS  Google Scholar 

  • Marsili, R., 1985, Monitoring chemical changes in Cheddar cheese during ripening by high performance liquid chromatography and gas chromatography techniques, J. Dairy Sci. 68:3155.

    Article  CAS  Google Scholar 

  • McGann, T.C.A., Mathiassen, A., and O’Connell, J.A., 1972, Applications of the Pro-Milk Mk. II. Part III. Rapid estimation of casein in milk and protein in whey, Lab. Pract. 21:628.

    CAS  Google Scholar 

  • McGugan, W.A., Emmons, D.B., and Larmond, E., 1979, Influence of volatile and nonvolatile fractions on intensity of Cheddar cheese flavor, J. Dairy Sci. 62:398.

    Article  CAS  Google Scholar 

  • McSweeney, P.L.H., and Fox, P.F., 1993, Cheese: Methods of Chemical Analysis, in: “Cheese: Chemistry, Physics and Microbiology,“ Vol. 1, General Aspects, 2nded., P.F. Fox, ed., Chapman

    Google Scholar 

  • McSweeney, P.L.H., Fox, P.F., and Law, J., 1993a, Contribution of cell wall-associated proteinases of Lactococcus to the primary proteolysis of ß-casein in Cheddar cheese, Milchwissenschafl 48:319.

    CAS  Google Scholar 

  • McSweeney, P.L.H., Fox, P.F., Lucey, J.A., Jordon, K.N., and Cogan, T.M., 1993b, Contribution of the indigenous microflora to the maturation of Cheddar cheese, Intern. Dairy J. 3:613.

    Article  CAS  Google Scholar 

  • McSweeney, P.L.H., Olson, N.F., Fox, P.F., Healy, A., and Hojrup, P., 1993c, Proteolytic specificity of chymosin on bovine αs1-casein, J. Dairy Res. 60:401.

    Article  CAS  Google Scholar 

  • McSweeney, P.L.H., Pochet, S., Fox, P.F., and Healy, A., 1994, Partial identification of peptides from the water-insoluble fraction of Cheddar cheese, J. Dairy Res. ,in press.

    Google Scholar 

  • Melachouris, N.P., and Tuckey, S.L., 1966, Changes in the proteins in Cheddar cheese made from milk heated at different temperatures, J. Dairy Sci. 49:800.

    Article  CAS  Google Scholar 

  • Mogensen, M.T.S., 1947, Determination of the degree of proteolytic decomposition in cheese, Assk. Alnarbs. Lantsbruk. Mejeri-Tradgardsinst. 279, cited from Chem. Abstr. ,1948,42:8987d.

    Google Scholar 

  • Moio, L., Di Luccia, A., and Addeo, F., 1989, Fast isoelectric-focusing of milk-proteins on small ultrathin polyacrylaande gels containing urea, Electrophoresis 10:533.

    Article  CAS  Google Scholar 

  • Moio, L., Chianese, L., Rivemale, M., and Addeo, F., 1992, Fast detection of bovine milk in Roquefort cheese with PhastSystem® by gel isoelectric focusing and immunoblotting, Lait 72:87.

    Article  CAS  Google Scholar 

  • Mojarro-Guerra, S.H., Amado, R., Arrigoni, E., and Solms, J., 1991, Isolation of low-molecular-weight taste peptides from Vachenin Mont d’Or cheese, J. Food Sci. 56:943.

    Article  CAS  Google Scholar 

  • Moore, S., and Stein, W.H., 1948, Photometric ninhydrin method for use in the chromatography of amino acids, J. Biol. Chem. 176:367.

    CAS  Google Scholar 

  • Moore, S., and Stein, W.H., 1954a, Procedure for the Chromatographie determination of amino acids on four percent cross-linked sulfonated polystyrene resins, J. Biol. Chem. 211:893.

    CAS  Google Scholar 

  • Moore, S., and Stein, W.H., 1954b, A modified ninhydrin reagent for the photometric determination of amino acids and related compounds, J. Biol. Chem. 211: 907.

    CAS  Google Scholar 

  • Morris, H.A., Holt, C., Brooker, B.E., Banks, J.M., and Manson, W., 1988, Inorganic constituents of cheese: analysis of juice from a one month old Cheddar cheese and the use of light and electron microscopy to characterize the crystalline phases, J. Dairy Res. 55:255.

    Article  CAS  Google Scholar 

  • Motoba, T., Hayashi, R., and Hata, T., 1970, Isolation of bitter peptides from tryptic hydrolyzate of casein and their chemical structure, Agric. Biol. Chem. 34:1235.

    Article  Google Scholar 

  • Mulvihill, D.M., and Fox, P.F., 1979, Proteolytic specificity of chymosin on bovine αs1-casein, J. Dairy Res. 46:641.

    Article  CAS  Google Scholar 

  • Natli, K.R.,and Ledford, R.A., 1972, Growth response of Lactobacillus casei variety casei to the proteolysis products in cheese during ripening, J. Dairy Sci. 56:710.

    Google Scholar 

  • Ney, K.H., 1985, N-Endstandige Aminosauren der Peptide aus reifem Cheddarkase, Milchwissenschaft 40:207.

    CAS  Google Scholar 

  • Nguyen, A.L., Luong, J.H.T., and Masson, T., 1990, Determination of nucleotides in fish tissues using capillary electrophoresis, Anal. Chem. 62: 2490.

    Article  CAS  Google Scholar 

  • Noomen, A., 1977a, Noordhollandse Meshanger cheese: a novel method for research on cheese ripening. 2. The ripening of the cheese, Neth. Milk Dairy J. 31:75.

    CAS  Google Scholar 

  • Noomen, A., 1977b, A rapid method for the estimation of the dissolved and the undissolved nitrogen compounds in cheese, Neth. Milk Dairy J. 31:163.

    CAS  Google Scholar 

  • Notte, E.La., Santoro, M., Leone, A. M and Vitagliano, M., 1980, Behaviour of free amino acids during ripening of pasta filata cheeses. I. Fior di Latte, Sci. Tec. Lanier o-Casearia 31:19, cited from Food Sci. Technol. Abstr. ,1980, l2:P2O47.

    Google Scholar 

  • Nunez, M., Garcia-Aser, C., Rodriguez-Martin, M. A., Medina, M., and Gaya, P., 1986, The effect of ripening and cooking temperature on proteolysis and lipolysis in Manchego cheese, Food Chem. 21:115.

    Article  CAS  Google Scholar 

  • O’Keeffe, A.M., Fox, P.F., and Daly, C, 1978, Proteolysis in Cheddar cheese: role of coagulant and starter bacteria, J. Dairy Res. 45:465.

    Article  Google Scholar 

  • O’Keeffe, R.B., Fox, P.F., and Daly, C., 1976, Contribution of rennet and starter proteases to proteolysis in Cheddar cheese, J. Dairy Res. 43:97.

    Article  Google Scholar 

  • O’Sullivan, M., and Fox, P.F., 1990, A scheme for partial fractionation of cheese peptides, J. Dairy Res. 57:135.

    Article  Google Scholar 

  • Ollikainen, P., 1990, Titration -a rapid method for the determination of proteolysis in cheese, J. Dairy Res. 57:149.

    Article  CAS  Google Scholar 

  • Omar, M.M., 1984, Microstructure, free amino acids and free fatty acids in Ras cheese, Food Chem. 15:19.

    CAS  Google Scholar 

  • Ordonez, J.A., and Burgos, J., 1977, Etude de la variete de fromage “Ulloa”. 1. Evolution de la flore micorbienne et des composants azotes au cours de la maturation, Lait 57:150.

    Article  Google Scholar 

  • Pearce, K.N., 1979, Use of fluorescence to determine the rate of release of the caseino-macropeptide in rennet treated milk, N.Z. J. Dairy Sci. Technol. 14: 233.

    CAS  Google Scholar 

  • Pearce, K.N., Karahalios, D., and Friedman, M., 1988, Ninhydrin assay for proteolysis in ripening cheese, J. Food Sci. 53:432.

    Article  CAS  Google Scholar 

  • Peterson, R.F., Nauman, L.W., and McMeekin, T.L., 1957, The separation of amino acid composition of a pure phosphopeptone prepared from ß-casein by the action of trypsin, J. Am. Chem. Soc. 80:95.

    Article  Google Scholar 

  • Pettersson, H.-E., and Sjostrom, G., 1975, Accelerated ripening of cheese: a method for increasing the number of lactic starter bacteria in cheese without detrimental effect to cheese making process, and its effect on the cheese ripening, J. Dairy Res. 42:313.

    Article  CAS  Google Scholar 

  • Pham, A.-M, and Nakai, S., 1984, Application of stepwise discriminant analysis to high pressure liquid chromatography profiles of water extract for judging ripening of Cheddar cheese, J. Dairy Sci.67:1390.

    Article  Google Scholar 

  • Polo, C., Ramos, M., and Sanches, R., 1985, Free ainino acids by high perfonnance liquid chromatography and peptides by gel electrophoresis in Mahon cheese during ripening, Food Chem. 16:85.

    Article  CAS  Google Scholar 

  • Polychroniadou, A., 1988, A simple procedure using trinitrobenzenesulphonic acid for monitoring proteolysis in cheese, J. Dairy Res. 55:585.

    Article  CAS  Google Scholar 

  • Polychroniadou, A., and Vlachos, J., 1979, Les acides amines du fromage télémé, La it 59:234.

    CAS  Google Scholar 

  • Poznanski, S., Habaj, B., Rymaszewski, J., and Rapazynski, T., 1966, Influence of different sUirter cultures on the protein breakdown in Edam cheese, Proc. 17th Intern. Dairy Congr., Munich, D: 555.

    Google Scholar 

  • Puchades, R., Lemieux, L., and Simard, R.E., 1989a, Evolution of free ainino acids during the ripening of Cheddar cheese containing added lactobacilli strains, J. Food Sci. 54:885.

    Article  CAS  Google Scholar 

  • Puchades, R., Lemieux, L., and Simard, R.E., 1989b, Sensitive, rapid and precise determination of L-glutamic acid in cheese using a flow injection system with immobilized enzyme column,J. Food Sci. 54:423.

    Article  CAS  Google Scholar 

  • Puchades, R., Lemieux, L., and Simard, R.E., 1990, Determination of free amino acids in cheese by flowinjection analysis with an enzyme reactor and chemiluminescence detector, J. Food Sci. 55:1555.

    Article  CAS  Google Scholar 

  • Ramos, M., Caceres, I., Polo, C, Alonso, L., and Juarez, M 1987, Effect of freezing on soluble nitrogen fraction of Cabrales cheese, Food Chem. 24:271.

    Article  CAS  Google Scholar 

  • Rank, T.C., Grappin, R., and Olson, N.F., 1985, Secondary proteolysis of cheese during ripening: A review, J. Dairy Sci. 68:801.

    Article  CAS  Google Scholar 

  • Reiter, B., Sorokin, Y., Pickering, A., and Hall, A.J., 1969, Hydrolysis of fat and protein in small cheeses made under aseptic conditions, J. Dairy Res. 36:65.

    CAS  Google Scholar 

  • Resmini, P., Pellegrino, L., Pazzaglia, C., and Hogenboom, J.A., 1985a, Free amino acids as an index of the quality of Parmigiano-Reggiana cheese, particularly grated products, Scienza Tec. Lattiero-Casearia

    Google Scholar 

  • Resmini, P., Pellegrino, L., Hogenboom, J.A., and Bertucciolo, M. 1985b, Investigation of the possibility of characterizing Provolone cheese by its free amino acid pattern, Scienza Tec. Lattiero-Casearia 39:81, cited from Food Sci. Technol. Abstr. ,1988, 2O:I2PI28.

    Google Scholar 

  • Reville, W.J., and Fox, P.F., 1978, Soluble protein in Cheddar cheese: A comparison of analytical methods, Irish J. Food Sci. Technol. 2:67’.

    CAS  Google Scholar 

  • Ruheman, S., 1910, Triketohydrinden hydrate, J. Chem. Soc. 98:2025.

    Google Scholar 

  • Salji, J.P., and Kroger, M., 1981, Proteolysis and lipolysis in ripening Cheddar cheese made with conventional bulk starter and with frozen concentrated direct-to-the-vat culture, J. Food Sci. 46:1345.

    Article  CAS  Google Scholar 

  • Samples, D.R., Richter, R.L., and Dill, C.W., 1984, Measuring proteolysis in Cheddar cheese slurries: comparison of Hull and trinitrobenzene sulfonic acid procedures, J. Dairy Sci. 67:60.

    Article  CAS  Google Scholar 

  • Santoro, M., and Faccia, M., 1991, Some peptides in Provolone cheese, Ital. J. Food Sci. 3:307.

    CAS  Google Scholar 

  • Santoro, M., Leone, A.M., La Notte, E., and Liuzzi, V.A., 1987, Fractionation of soluble nitrogen in Provolone cheese, I, Milchwissenschaft 42:709.

    CAS  Google Scholar 

  • Satake, K., Okuyama, T., Ohashi, M., and Shineda, T., 1960, The spectrophotometric determination of amine, amino acid and peptide with 2,4,6-trinitrobenzene 1-sulfonic acid, J. Biochem (Tokyo)47:654.

    CAS  Google Scholar 

  • Sciancalepore, V., and Alviti, F.S., 1987, A dialysis method for measuring cheese ripening, Milchwissenschaft 42: 220.

    Google Scholar 

  • Sciancalepore, V., and Longone, V., 1988, Rapid methods for measuring the degree of proteolysis as cheese ripening index, Milchwissense haft 43:357.

    CAS  Google Scholar 

  • Shalabi, S.I., and Fox, P.F., 1988, Electrophoretic analysis of cheese: comparison of methods, Irish J. Food Sci. Technol. 11:135.

    Google Scholar 

  • Shindo, K., Sakurada, K., Niki, R., and Arima, S., 1980, Studies on immobilized chymosin. 5. Experiments in cheesemaking with immobilized chymosin., Milchwissenschafl 35:527.

    CAS  Google Scholar 

  • Singh, A., and Ganguli, N.C., 1972, Changes in peptides and amino acids in cheese during ripening, Milchwissenschaft 27:412.

    CAS  Google Scholar 

  • Singh, T.K., Fox, P.F., Højrup, P., and Healy, A., 1994, A scheme for the fractionation of cheese nitrogen and identification of principal peptides, Intern. Dairy J. 4:111.

    Article  Google Scholar 

  • Smith, A.M., and Nakai, S., 1990, Classification of cheese varieties by multivariate analysis of HPLC profiles, Can. Inst. Food Sci. Technol. J. 23:53.

    Google Scholar 

  • St. Martin, M., and Paquin, P., 1990, Ion exchange fast protein liquid chromatography: optimization of the purification of caseins using a non-denaturing detergent, J. Dairy Res. 57:63.

    Article  Google Scholar 

  • Stadhouders, J., 1960, The hydrolysis of protein during ripening of Dutch cheese. The enzymes and bacteria involved, Neth. Milk Dairy J. 14:83.

    CAS  Google Scholar 

  • Storgards, T., and Lindqvist, B., 1953, Untersuchungen über die Käsereifung. I. Chromatographische Untersuchungen über das Vorkommen von Aminosäuren in verschiedenen Käsesorten Milchwissenschafl 8:5.

    CAS  Google Scholar 

  • Strange E.D., Malin, E.L., Van Hekken, D.L. and Basch, J.J., 1992, Chromatographie and electrophoretic methods used for analysis of milk proteins, J. Chromatogr. 624:81.

    Article  CAS  Google Scholar 

  • Tegtmeyer, P., Schwartz, M., Collins, J.K., and Rundell, K., 1975, Regulation of tumor antigen synthesis by Simian virus 40 Gene A, J. Virol. 16:168.

    CAS  Google Scholar 

  • Thibault, P., Pleasance, S., and Laycock, M.V., 1991, Analysis of paralytic shellfish poisons by capillary electrophoresis, J. Chromatogr. 542:483.

    Article  CAS  Google Scholar 

  • Tieleman, A.E., and Warthesen, J.J., 1991, Comparison of three extraction procedures to characterize Cheddar cheese proteolysis, J. Dairy Sci. 74:3686.

    Article  CAS  Google Scholar 

  • Tokita, F., and Hosono, A., 1968, An investigation about Limburger cheese ripening by gel filtration on Sephadex, Milchwissenschaft 23:758.

    Google Scholar 

  • Tokita, F., and Nakanishi, T., 1962, On the distributive patterns of lower molecular weight peptides in cheese, Milchwissenschaft 17:198.

    Google Scholar 

  • Trieu-Cuot, P., and Gripon, J.-C., 1982, A study of proteolysis during Camembert cheese ripening using isoelectric focusing and two-dimensional electrophoresis, J. Dairy Res. 49:501.

    Article  CAS  Google Scholar 

  • Tsarichenko, A.P., 1966, New ninhydrin reagent for quantitative determination of amino acids in eluates in column chromatography, Nauch. Tr. Krasnodar. Gos. Pedogog. Inst. No. 70:86, cited from Chem. Abstr. ,1967, 67:79479C.

    Google Scholar 

  • Tunick, M.H., Mackey, K.L., Shieh, J.J., Smith, P.W., Cooke, P., and Malin, E.L., 1993, Rheology and microstructure of low-fat Mozzarella cheese, Intern. Dairy J. 649.

    Google Scholar 

  • Ucuncu, M., 1981, Study of free amino acids during ripening of Turkish white cheese (Feta cheese) from cows’ and ewes’ milk, Molkerei-Zeitung Wett de Milch. 35:634, cited from Food Sci. Technol. Abstr. ,1982, 14:2P298.

    Google Scholar 

  • Udenfried, S., Stein, S., Bohlen, P., Darman, W., Leimgruber, W., and Weigele, M., 1972, Fluorescamine: A reagent for assay of amino acids, peptides, proteins and primary amines in the picomole range, Science 178:871.

    Google Scholar 

  • Vakaleris, D.G., and Price, W.V., 1959, A rapid spectrophotometric method for measuring cheese ripening, J. Dairy Sci. 42:264.

    Article  CAS  Google Scholar 

  • Vakaleris, D.G., Olson, N.F., Price, W.V., and Knight, S.G., 1960, A study of the ripening of Dariworld and Cheddar cheese with special emphasis on proteolysis, J. Dairy Sci. 43:1058.

    Article  CAS  Google Scholar 

  • van Ilooydonk, A.C.M., and Olieman, C., 1982, A rapid sensitive high performance liquid Chromatographie method of following the action of chymosin in milk, Neth. Milk Dairy J. 36:153.

    Google Scholar 

  • Venema, D.P., Herstel, H., and Elenbaas, ILL., 1987, Determination of ripening time of Edam and Gouda cheese by chemical analysis, Neth. Milk Dairy J. 41:215.

    Google Scholar 

  • Vijayalakshmi, M.A., Lemieux, L., and Amiot, J., 1986, High performance size exclusion liquid chromatography of small molecular weight peptides from protein hydrolysates using methanol as mobile phase additive, J. Liquid Chromatogr. 9:3559.

    Article  CAS  Google Scholar 

  • Visser, F.M.W., 1977a, Contribution of enzymes from rennet, starter bacteria and milk to proteolysis and flavour development in Gouda cheese. 3. Protein breakdown: analysis of the soluble nitrogen and amino acid fractions, Neth. Milk Dairy J. 31:210.

    CAS  Google Scholar 

  • Visser, F.M.W., 1977b, Contribution of enzymes from rennet, starter bacteria and milk to proteolysis and flavour development in Gouda cheese. 2. Development of bitterness and chese flavour, Neth. Milk Dairy J. 31:265.

    CAS  Google Scholar 

  • Visser, S., Slangen, K.J., and Hup, G., 1975, Some bitter peptides from rennet-treated casein. A method for their purification, utilizing Chromatographie separation on silica gel, Neth. Milk Dairy J. 29:319.

    CAS  Google Scholar 

  • Visser, S., Hup, G., Exterkate, F.A., and Stadhouders, J., 1983a, Bitter peptides in cheese. 2. Model studies on the formation and degradation of bitter peptides by proteoly tic enzymes from calf rennet, starter cells and starter cell fractions, Neth. Milk Dairy J. 37:169

    CAS  Google Scholar 

  • Visser, S., Slangen, K.J., Hup, G., and Stadhouders, J., 1983b, Bitter flavour in cheese. 3. Comparative gel-chromatographic analysis of hydrophobic peptide fractions from twelve Gouda-type cheeses and identification of bitter peptides isolated from a cheese made with Streptococcus cremoris strain HP, Neth. MilkDaityJ. 37:181.

    CAS  Google Scholar 

  • Vreeman, H.J., Visser, S., Slangen, C.J., and van Riel, J.A.M., 1986, Characterization of bovine κ-casein fractions and the kinetics of chymosin-induced macropeptide release from carbohydrate-free and carbohydrate-containing fractions determined by high performance gel permeation chromatography, Biochem. J. 240:87.

    CAS  Google Scholar 

  • Weaver, J.C., Kroger, M., and Thompson, M.P., 1978, Free amino acid and rheological measurement on hydrolysed lactose Cheddar cheese during ripening, J. Food Sci. 43:579.

    Article  CAS  Google Scholar 

  • Weigele, M., de Bernardo, S.L., Tengi, J.P., and Leimgruber, W., 1972, A novel reagent for the fluorometric assay of primary amines, J. Am. Chem. Soc. 94:5927.

    Article  CAS  Google Scholar 

  • Wilkinson, M.G., 1992, “Studies on the Acceleration of Cheddar Cheese Ripening,“ Ph.D. Thesis, National University of Ireland, Cork.

    Google Scholar 

  • Wilkinson, M.G., Guinee, T.P., O’Callaghan, D.M., and Fox, P.F., 1992, Effect of commercial enzymes on proteolysis and ripening in Cheddar cheese, Lait 72:449.

    Article  CAS  Google Scholar 

  • Wood, A.F., Aston, J.W., and Douglas, G.K., 1985, The determination of free amino acids in cheese by capillary column gas-liquid chromatography, Aust. J. Dairy Technol. 40:166.

    CAS  Google Scholar 

  • Young, P.M., Astephen, N.E., and Wheat, T.E., 1992, Effects of pH and buffer composition on peptide separations by high performance liquid chromatography and capillary electrophoresis, LC-GC 10:26.

    CAS  Google Scholar 

  • Yvon, M., Chabanet, C, and Pelisser, J.-P., 1989, Solubility of peptides in trichloroacetic acid (TCA) solutions, Intern. J. Peptide Protein Res. 34:166.

    Article  CAS  Google Scholar 

  • Zeece, M., 1992, Capillary electrophoresis: a new analytical tool for food science, Trends Food Sci. Technol. 3:6.

    Article  CAS  Google Scholar 

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© 1995 Springer Science+Business Media New York

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Fox, P.F., McSweeney, P.L.H., Singh, T.K. (1995). Methods for Assessing Proteolysis in Cheese During Maturation. In: Malin, E.L., Tunick, M.H. (eds) Chemistry of Structure-Function Relationships in Cheese. Advances in Experimental Medicine and Biology, vol 367. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1913-3_10

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  • DOI: https://doi.org/10.1007/978-1-4615-1913-3_10

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