Abstract
Demyelination following central nervous system (CNS) infection with the neurotropic Coronavirus, mouse hepatitis virus, strain JHM (MHV-JHM) has been shown to be dependent on the immune response of the host (Perlman, 1998). Although controversial, both CD4 and CD8 T cells are thought to contribute to demyelination (Perlman, 1998). However, the mechanisms by which each T cell subset contributes to demyelination remains unknown. To address this issue, the following questions were raised. First, what is the magnitude of the T cell response during immune-mediated demyelination? Second, what is the timing of the immune response leading to demyelination? Third, what are the roles of CD4 and CD8 T cells — in particular virus-specific T cells — during MHV-JHM-induced demyelination? In order to address these questions, a previously described adoptive transfer system (Wu and Perlman, 1999) was utilized to measure the quantity of virus-specific T cells during the process of immune-mediated demyelination. Furthermore, the individual contributions of CD4 or CD8 T cells to MHV-JHM-induced demyelinating disease were determined by depletion studies.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Bergmann, C. C., Altaian, J. D., Hinton, D., Stohlman, S. A. (1999). Inverted immunodominance and impaired cytolytic function of CD8+ T cells during viral persistence in the central nervous system. J. Immunol. 163, 3379–3387.
Lane, T. E., Liu, M. T., Chen, B. P., Asensio, V. C., Samawi, R. M., Paoletti, A. D., Campbell, I. L., Kunkel, S. L., Fox, H. S., Buchmeier, M. J. (2000). A central role for CD4+ T-cells and RANTES in virus-induced central nervous system inflammation and demyelination. J. Virol. 74, 1415–1424.
Perlman, S. (1998). Pathogenesis of Coronavirus-induced infections: Review of pathological and immunological aspects. Adv. Expt. Med. Biol. 440, 503–513.
Pewe, L., Heard, S. B., Bergmann, C. C., Dailey, M. O., Perlman, S. (1999). Selection of CTL escape mutants in mice infected with a neurotropic Coronavirus: Quantitative estimate of TCR diversity in the infected CNS. J. Immunol. 163, 6106–6113.
Stohlman, S. A., Bergmann, C. C., Lin, M. T., Cua, D. J., Hinton, D. R. (1998). CTL effector function within the central nervous system requires CD4+ T Cells. J. Immunol. 160, 2896–2904.
Wu, G., Dandekar, A., Pewe, L., Perlman, S. CD4 and CD8 T cells have redundant but not identical roles in virus-induced demyelination. J. Immunol. in press.
Wu, G. F., Perlman, S. (1999). Macrophage infiltration, but not apoptosis, is correlated with immune-mediated demyelination following murine infection with a neurotropic Coronavirus. J. Virol. 73, 8771–8780.
Xue, S., Sun, N., van Rooijen, N., Perlman, S. (1999). Depletion of blood-borne macrophages does not reduce demyelination in mice infected with a neurotropic Coronavirus. J. Virol. 73, 6327–6334.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this chapter
Cite this chapter
Wu, G.F., Dandekar, A.A., Pewe, L., Perlman, S. (2001). The Role of CD4 and CD8 T Cells in MHV-JHM-Induced Demyelination. In: Lavi, E., Weiss, S.R., Hingley, S.T. (eds) The Nidoviruses. Advances in Experimental Medicine and Biology, vol 494. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1325-4_51
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1325-4_51
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5498-7
Online ISBN: 978-1-4615-1325-4
eBook Packages: Springer Book Archive