Skip to main content
SpringerLink
Log in

Somatic Mutation and Recombination Test in Drosophila Used for Biomonitoring of Environmental Pollutants

  • Chapter
Biomonitors and Biomarkers as Indicators of Environmental Change 2

Part of the book series: Environmental Science Research ((ESRH,volume 56))

Abstract

The somatic mutation and recombination test (SMART) in Drosophila melanogaster has been designed to detect genetic damage in a rapid and inexpensive way. It is an in vivo system that uses an eukaryotic organism with metabolic machinery similar to that found in mammalian cells. Two different systems have been widely explored, i.e. the wing spot test and the eye spot test. Both of them are based on the fact that during the early embryonic development of the fly, groups of cells (imaginal discs) are set apart and proliferate mitotically during the whole larval period until differentiation into the different structures of an adult fly (Guzamán-Rincón and Graf, 1995).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Benedict, W.F., A. Benerjee, K.K. Kangalingam, D.R. Dansie, R.E. Kouri, and C.J. Henry. 1984. Increased sister chromatid exchange in bone-marrow cells to exposed to whole cigarette smoke. Mutation Res. 136: 73–80.

    Article  CAS  Google Scholar 

  • Crebelli, R., L. Conti, S. Fuselli, P. Leopardi, A. Zijno, and A. Carere. 1991. Further studies on the comutagenic activity of cigarette smoke condensate. Mutation Res. 259: 29–36.

    Article  CAS  Google Scholar 

  • Dapkus, J., and D.J. Merrel. 1977. Chromosomal analysis of DDT resistance in a long-term selected population of Drosophila melanogaster. Genetics 87: 685–697.

    CAS  Google Scholar 

  • De Flora, S., R. Balansky, L. Gasparini, and A. Camoriano. 1995. Bacterial mutagenicity of cigarette smoke and its interaction with ethanol. Mutagenesis 10: 47–52.

    Article  Google Scholar 

  • DeMarini, D.M. 1998. Mutation spectra of complex mixtures. Mutation Res. 411: 11–18.

    Article  CAS  Google Scholar 

  • DeMarini, D.M., M.L. Shelton, and J.G. Levine. 1995. Mutation spectrum of cigarette smoke condensate in Salmonella: comparison to mutations in smoking-associated tumors. Carcinogenesis 16: 2535–2542.

    Article  CAS  Google Scholar 

  • Delgado-Rodriguez, A., R. Ortiz-Martello, U. Graf, R. Villalobos-Pietrini, and S. Gomez-Arroyo. 1995. Genotoxic activity of environmentally important polycyclic aromatic hydrocarbons and their nitro derivates in the wing spot test o Drosophila melanogaster. Mutation Res. 341: 235–247.

    Article  Google Scholar 

  • Frei, H., and F.E. Würgler. 1988. Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positibe, negative or inconclusive results. Mutation Res. 203: 297–308.

    Article  CAS  Google Scholar 

  • Frei, H., and F.E. Würgler. 1995. Optimal experimental design and sample size for the statistical evaluation of data from somatic mutation and recombination test (SMART) in Drosophila. Mutation Res. 334: 247–258.

    Article  CAS  Google Scholar 

  • Frei, H., H. Clements, D. Howe, and F.E. Wurgler. 1992a. The genotoxicity of the anti-cancer drug mitox-antrone in somatic and germ cells of Drosophila melanogaster. Mutation Res. 279: 21–33.

    Article  CAS  Google Scholar 

  • Frei, H., J. Liithy, J. Brauchli, U. Zweifel, F.E. Wtirgler, and C. Schlatter. 1992b. Structure/activity relationship of the genotoxic potencies of sixteen pyrrolizidine alkaloids assayed for the induction of somatic mutation and recombination in wing cells of Drosophila melanogaster. Chem. Biol. Interact. 83: 1–22.

    Article  CAS  Google Scholar 

  • Frolich, A., and F.E. Wtirgler. 1989. New tester strains with improved bioactivation capacity for the Drosophila wing spot test. Mutation Res. 216: 179–187.

    Article  CAS  Google Scholar 

  • FrOlich, A., and F.E. Wurgler. 1990a. Drosophila wing spot test: improved detectability of genotoxicity of polycyclic aromatic hydrocarbons. Mutation Res. 234: 71–80.

    Article  CAS  Google Scholar 

  • Frolich, A., and F.E. Wtirgler. 1990b. Genotoxicity of ethyl carbamate in the Drosophila wing spot test: dependence on genotype-controlled metabolic capacity. Mutation Res. 244: 201–208.

    Article  CAS  Google Scholar 

  • Graf, U. 1986. Temperature effect on mwh expression in the wing somatic mutation and recombination test in Drosophila melanogaster. Drosophila Inform. Serv. 63: 65.

    Google Scholar 

  • Graf, U. 1994. Analysis of the relationship between age of larvae at mutagen treatment and frequency of size of spots in the wing somatic mutation and recombination test of Drosophila melanogaster. Experientia. 51: 168–174.

    Article  Google Scholar 

  • Graf, U., and D. Singer. 1989. Somatic mutation and recombination test in Drosophila melanogaster (wing spot test): Effects of extracts of airborne particulate matter from fire-exposed and non fire-exposed building ventilation filters. Chemosphere 19: 1094–1097.

    Article  Google Scholar 

  • Graf, U., and D. Singer. 1992. Genotoxicity testing of promutagens in the wing somatic mutation and recombination test in Drosophila melanogaster. Rev. Int. Contam. Ambient. 8: 15–27.

    CAS  Google Scholar 

  • Graf U., and N. van Schaik. 1992. Improved high bioactivation cross for the wing somatic mutation and recombination test in Drosophila melanogaster. Mutation Res. 271: 59–67.

    Article  CAS  Google Scholar 

  • Graf, U., and F.E. Würgler. 1986. Investigation of coffee in Drosophila genotoxicity tests. Food Chem. Toxicol. 24: 835–842.

    Article  CAS  Google Scholar 

  • Graf, U., and F.E. Wilrgler. 1996. The somatic white-ivory eye spot test does not detect the same spectrum of genotoxic events as he wing somatic mutation and recombination test in Drosophila melanogaster. Environ. Mol. Mutagen. 27: 219–226.

    Article  CAS  Google Scholar 

  • Graf, U., C.B. Hall, and N. van Schaik. 1990. On the use of excision repair defective cells in the wing somatic mutation and recombination test in Drosophila melanogaster. Mutation Res. 271: 59–67.

    Google Scholar 

  • Graf, U., S. Heo, and 0. Olvera Ramirez 1992. The genotoxicity of chromium (VI) oxide in the wing spot test of Drosophila melanogaster is over 90% due to mitotic recombination. Mutation Res. 266: 197–203.

    Article  CAS  Google Scholar 

  • Graf, U., A. Alonso-Moraga, R. Castro, and E. Díaz-Carrillo. 1994. Genotoxicity testing of different types of beverages in the Drosophila wing somatic mutation and recombination test. Food Chem. Toxicol. 32: 423–430.

    Article  CAS  Google Scholar 

  • Graf, U., F.E. Würgler, A.J. Katz, H. Frei, H. Juon, C.B. Hall, and P.G. Kale. 1984. Somatic mutation and recombination test in Drosophila melanogaster. Environ. Mutagen. 6: 153–188.

    Article  CAS  Google Scholar 

  • Guzmán-Rincón, J., and U. Graf. 1995. Drosophila melanogaster Somatic Mutation and Recombination Test as a biomonitor., in: EM. Butterworth, L.D. Corkum, and J. Guzmán-Rincón (Eds). Biomonitors and Biomarkers as Indicators of Environmental Change: a Handbook, Plenum Press, New York pp. 169–181.

    Google Scholar 

  • Guzmán-Rincón, J., J. Espinosa, and U. Graf. 1998. Analysis of the in vivo nitrosation capacity of the larvae used in the wing somatic mutation and recombination test of Drosophila melanogaster. Mutation Res. 412: 69–81.

    Article  Google Scholar 

  • Guzmán-Rincón, J., U. Graf, A. Varela, R. Policroniades, and A. Delfin. 1994. Somatic mutation and recombination induced by fast neutrons in the wing spot test of Drosophila melanogaster. Rev. Int. Contam. Ambient. 10. suppl. 1: 29–30.

    Google Scholar 

  • Hallstrom, I., and A. Blanck. 1985. Genetic variation in cytochrome P450 system in Drosophila melanogaster. L. Chromosomal determination of some cytochrome P450-dependent reactions. Chem. Biol. Interactions 56: 157–171.

    CAS  Google Scholar 

  • Hailstorm, I., and R. Grafstrom. 1981. The metabolism of drugs and carcinogens in isolated subcellular fractions of Drosophila melanogaster. II. Enzyme induction and metabolism of benzo(a)pyrene. Chem. Biol. Interact. 34: 1455–1459.

    Google Scholar 

  • Hess, R.D., and G. Brandner. 1996. DNA damage by filtered, tar-and aerosol-free cigarette smoke in rodent cells: a novel evaluation. Toxicol. Lett. 88: 9–13.

    CAS  Google Scholar 

  • Hecht, S.S., and D. Hoffmann 1988. Tobacco-specific nitrosamines, an important group of carcinogens in tobacco and tobacco smoke. Carcinogenesis. 9: 875–884.

    Article  CAS  Google Scholar 

  • Hetch, S.S., A. Riverson, J. Braley, J. Dibello, J D. Adams, and D. Hoffmann. 1986. Induction of oral cavity tumors in F344 rats by tobacco. specific nitrosamines and snuff. Cancer Res. 46: 4162–4166.

    Google Scholar 

  • Hoffmann, D., A. Castonguay, A. Riverson, and S.S. Hecht. 1981. Comparative carcinogenicity and metabolism of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine in Syrian golden hamsters. Cancer Res. 41: 2386–2393.

    CAS  Google Scholar 

  • Hsu, T.C., L.M. Cherry, C. Bucana, L.R. Shirley, and C.G. Gairola. 1991. Mitosis-arresting effects of cigarette smoke condensate on human lymphoid cell lines. Mutation Res. 259: 67–78.

    Article  CAS  Google Scholar 

  • IARC (International Agency for Research on Cancer). Tobacco smoking. IARC. 1986. Monographs on the evaluation of the carcinogenic risk of chemicals to humans, Vol. 38, pp. 37–375. Lyon, France.

    Google Scholar 

  • Katz, A.J., and T.A. Foley. 1993. Effect of temperature on frequencies of spots in Drosophila wing-spot assay. Environ. Mol. Mutagen. 22: 54–58.

    Article  CAS  Google Scholar 

  • Kramers, P.G.N., B. Bissumbhar, and H.C.A. Moout. 1985. Studies with gaseous mutagens in Drosophila melanogaster, in: M.D. Waters, S.S. Sandhu, J. Lewtas and L. Claxton (Eds.) Short-Term Bioassays in the analysis of Complex Environmental Mixtures, IV, Plenum, New York, pp. 65–73.

    Google Scholar 

  • Lindsley, D.L., and G. Zimm 1992. The genome of Drosophila melanogaster. Academic Press, San Diego. 1133pp.

    Google Scholar 

  • Nakamura, KY., K. Kawai, H. Furukawa, T. Matsuo, K. Shimoi, I. Tomita, and Y. Nakamura. 1997. Suppressing effects of S-methyl methanethiosulfonate and diphenyl disulfide on mitomycin C-induced somatic mutation and recombination in Drosophila melanogaster and micronuclei in mice. Mutation Res. 385: 41–46.

    Article  CAS  Google Scholar 

  • Olvera, 0., S. Zimmering, M.P. Cruces, E. Pimentel, C. Arceo, J. Guzman, and M.E. de la Rosa. 1997. Antimutagenesis in somatic cells of Drosophila as monitored in the wing spot test. Food Factors for Cancer Prevention, Ohigashi, Osawa, Terao, Watanabe, Yoshikawa Eds. Springer-Verlag Tokyo pp. 567–571.

    Google Scholar 

  • Ramel, C., and J. Magnusson. 1992. Modulation of genotoxicity in Drosophila. Mutation Res. 267: 221–227.

    Article  CAS  Google Scholar 

  • Riverson, A., D. Hoffmann, B. Prokopczyk, S. Amin, and S.S. Hecht. 1988. Induction of lung and exocrine pancreas tumours in F344 rats by tobacco-specific and areca-derived N’nitrosamines. Cancer Res. 48: 6912–6917.

    Google Scholar 

  • Van Schaik, N., A. Grant, I. Rubenchik, and U. Graf. 1984. Use of Drosophila test systems for genotoxicity testing of herbal teas. Inmunol. Hematol. Res. 3: 199–202.

    Google Scholar 

  • Vogel, E.W., M.J.M. Nivard, and J.A. Zijlstra. 1991. Variation of spontaneous and induced mitotic recombination in different Drosophila populations: a pilot study on the effects of poly-aromatic hydrocarbons in six newly constructed tester strains. Mutation Res. 250: 291–298.

    Article  CAS  Google Scholar 

  • Vogel, E.W., and J.A. Zijlstra. 1987. Mechanistic and methodological aspects of chemically-induced somatic mutation and recombination in Drosophila melanogaster. Mutation Res. 182: 243–264.

    Article  CAS  Google Scholar 

  • Würgler, EE., and E.W. Vogel. 1986. In vivo mutagenicity testing using somatic cells of Drosophila melanogaster. In F.J. de Serres (Ed) Chemical Mutagens, Principles and Methods for Their Detection. Vol. 10 Plenum Press, New York, pp. 1–72.

    Google Scholar 

  • Zambrano, E, J. Guzmán-Rincón, L. Paredes-Gutierrez, and A. Delfin-Loya. 1998. Influencia de la edad larval en la inducci6n de la mutacion y recombinacion somatica con neutrones de reactor en Drosophila melanogaster. In preparation.

    Google Scholar 

  • Zordan M., M. Osti, S.S. Pavanello, R. Costa, and A.G. Levis. 1994. Relationship between benzo(a)pyreneDNA adducts and somatic mutation and recombination in Drosophila melanogaster. Environ Mol. Mutagen. 23: 171–178.

    Article  CAS  Google Scholar 

  • Zordan M., U. Graf, D. Singer, C. Beltrame, L. Della Valle, M. Osti, R. Costa, and A.G. Levis. 1991. T“e genotoxicity of nitrolotriacetic acid (NTA) in a somatic mutation and recombination test it Drosophila melanogaster. Mutation Res. 262: 253–261.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, ININ, Km.36.5 Carretera Mexico-Toluca, Salazar, CP 05245, Edo. de México, México

    Judith Guzmán-Rincόn & Patricia Ramίrez-Victoria

  2. Oncological Research Unit, Oncology Hospital, National Medical Center S-XXI, México, D.F 06720, México

    Luis Benitez

Authors
  1. Judith Guzmán-Rincόn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patricia Ramίrez-Victoria
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Luis Benitez
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute for River Research International, Rochester, Michigan, USA

    Frank M. Butterworth

  2. Donaukraft Engineering, Vienna, Austria

    Amara Gunatilaka

  3. Universidad Nacional Autonoma de Mexico, Cuidad Universitaria, Mexico

    Maria Eugenia Gonsebatt

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer Science+Business Media New York

About this chapter

Cite this chapter

Guzmán-Rincόn, J., Ramίrez-Victoria, P., Benitez, L. (2001). Somatic Mutation and Recombination Test in Drosophila Used for Biomonitoring of Environmental Pollutants. In: Butterworth, F.M., Gunatilaka, A., Gonsebatt, M.E. (eds) Biomonitors and Biomarkers as Indicators of Environmental Change 2. Environmental Science Research, vol 56. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1305-6_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-1305-6_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5488-8

  • Online ISBN: 978-1-4615-1305-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation