Abstract
Regulation of energy production is fundamental to the survival and propagation of any cell type. What makes the preimplantation mammalian embryo so fascinating to study is the fact that the embryo undergoes major changes in its physiology and gene expression profiles during development. As the fertilised oocyte develops and differentiates into the blastocyst, embryonic genes are successively turned on (with the concomitant destruction of maternally derived mRNAs). Subsequently there is a growing energy demand as mitoses and biosynthesis increase post-embryonic-genome activation and as the blastocoel subsequently forms (through the activity of Na/K ATPase in the trophectoderm). Concomitantly, there are major changes in the regulation and relative activities of energy generating pathways. Of clinical interest is the fact that should an embryo at any stage of development have substantially altered energy production, i.e. if the flux of a specific nutrient through a metabolic pathway alters to a significant degree, even for a brief period, then this is associated with significantly impaired development in culture and reduction of viability post-transfer. Clearly it is in our interest to understand how the preimplantation embryo regulates its energy production, and to develop culture systems that best support an ‘optimal’ metabolism. Furthermore, it is evident that analysis of embryo metabolism is an appropriate means of assessing embryonic health and predicting subsequent viability. The task ahead is to determine the optimal range of metabolic functions that reflect viability at successive stages of embryo development.
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Regulation of energy production is fundamental to the survival and propagation of any cell type. What makes the preimplantation mammalian embryo so fascinating to study is the fact that the embryo undergoes major changes in its physiology and gene expression profiles during development. As the fertilised oocyte develops and differentiates into the blastocyst, embryonic genes are successively turned on (with the concomitant destruction of maternally derived mRNAs). Subsequently there is a growing energy demand as mitoses and biosynthesis increase post-embryonic-genome activation and as the blastocoel subsequently forms (through the activity of Na/K ATPase in the trophectoderm). Concomitantly, there are major changes in the regulation and relative activities of energy generating pathways. Of clinical interest is the fact that should an embryo at any stage of development have substantially altered energy production, i.e. if the flux of a specific nutrient through a metabolic pathway alters to a significant degree, even for a brief period, then this is associated with significantly impaired development in culture and reduction of viability post-transfer. Clearly it is in our interest to understand how the preimplantation embryo regulates its energy production, and to develop culture systems that best support an ‘optimal’ metabolism. Furthermore, it is evident that analysis of embryo metabolism is an appropriate means of assessing embryonic health and predicting subsequent viability. The task ahead is to determine the optimal range of metabolic functions that reflect viability at successive stages of embryo development.
Changes in Metabolism with Development
The fertilised oocyte exhibits low utilisation of oxygen [1] and has a limited capacity to utilise glucose as an energy source [2]. In fact, glycolytic activity is restricted in the fertilised oocyte and early embryo by the allosteric regulation of phosphofructokinase (PFK) due to a high ATP:ADP ratio [3]. High ratio of these nucleotides is a consequence of the low energy demands at these stages, reflecting the dormant status of the oocyte from which it was derived. Consequently, in the absence of amino acids, the early embryo generates its energy from low levels of oxidation of pyruvate (fertilised oocyte) and lactate (from the 2-cell stage onwards), derived directly from oviduct fluid (and the cumulus cells in the case of the oocyte [4–6]) (Fig. 1). However, the early embryo can also utilise the malate-aspartate shuttle, and in the presence of specific amino acids, e.g. aspartate, the embryo can utilise lactate as an energy source through the conversion of lactate to pyruvate [7]. In the immediate post-fertilisation period of mouse zygotes, there is an absolute requirement for oxidative metabolism of pyruvate to support the completion of the second meiotic division and subsequent development [8].
With an increase in cell divisions and biosynthesis following the activation of the embryonic genome, the formation of a transporting epithelium when the morula is formed, and subsequent creation of the blastocyst, the need for cellular energy increases dramatically. Consequently there is a decline in the ATP:ADP ratio (and a dramatic increase in AMP levels) as ATP is readily consumed, thereby alleviating inhibition on PFK and facilitating the flux of glucose through glycolysis. However, the overall regulation of carbon flux through glycolysis is also regulated at a number of sites (Fig. 2).
Interestingly, although the blastocyst exhibits a much higher oxygen consumption than the cleavage stage embryo [9–11], the late stage embryo does not oxidise all of the glucose consumed, but rather converts approximately 50 % (somewhat species-dependent) of glucose carbon to lactate, this even in the presence of adequate oxygen for complete oxidation of the glucose. This latter metabolic phenomenon is referred to as ‘aerobic glycolysis’ (not to be confused with anaerobic glycolysis found in tissues such as muscle when oxygen is limited). Aerobic glycolysis is a trait found in cancers and rapidly dividing cells, a phenomenon also known as the Warburg effect [12, 13] (Fig. 2).
What Is the Role of Aerobic Glycolysis/Warburg Effect in the Blastocyst?
In the blastocyst of primates and rodents, high levels of aerobic glycolysis, have been interpreted as the embryos adaptation to its imminent invasion of the endometrium, which remains avascular for a period of up to 12 h, and will therefore be relatively anoxic [14, 15]. During the initial stages of blastocysts invasion, glycolysis will plausibly be the predominant means of generating energy. However, this may not be the sole explanation for the high levels of glycolysis observed in blastocysts of domestic animals, as they do not ‘implant’ for a further week. An alternative explanation for the rather idiosyncratic metabolism of the blastocyst was proposed by Gardner in 1998 [16]. A high level of aerobic glycolysis is a common characteristic of proliferating cells and tumours, with which the mammalian blastocyst shares several traits [17–19]. As well as being used to generate energy for increased mitoses and blastocoel expansion, high levels of glucose utilisation will be required for the synthesis of triacylglycerols and phospholipids and as a precursor for complex sugars of mucopolysaccharides and glycoproteins. Glucose metabolised by the pentose phosphate pathway (PPP) will generate ribose moieties required for nucleic acid synthesis and the NADPH and carbon skeletons required for the biosynthesis of lipids and other complex molecules [18, 20]. NADPH is also required for pathways of one carbon metabolism, which are vital for the methylation of various intracellular substrates, including DNA. It is now well appreciated that embryos undergo dramatic changes in the epigenetic modifications of their genomes including DNA methylation during preimplantation development [21]. NADPH is also required for the reduction of intracellular glutathione, an important antioxidant for the embryo [22]. Interestingly, although the absolute amount of glucose metabolised through the PPP increases with development [23], the percentage of the total glucose consumed which is metabolised through this pathway appears to be lowest at the blastocyst stage [24]. This again raises the question as to why such high levels of aerobic glycolysis are required? It is plausible that high levels of aerobic glycolysis, such as that observed in the mammalian blastocyst, will ensure that there is sufficient substrate and cytosolic ATP available for biosynthetic pathways, such as the synthesis of nucleic acids, proteins and new membranes, at the required times during cellular proliferation [25, 26]. This in turn suggests that there are times within the cell cycle when the PPP is more active than others (Fig. 2). Analysis of embryo energy metabolism with respect to cell cycle has not been thoroughly analysed, but it is speculated that at specific times during the cell cycle there will be increased energy demands, particularly during cytokinesis.
A further explanation for the high levels of aerobic glycolysis by the blastocyst could lie in the activity of mitochondrial shuttles in the oxidation of hydrogen equivalents formed through glycolysis. The malate-aspartate is one such shuttle that allows cytosolic NADH to be exchanged for NAD+ that is derived from the mitochondrion. Once within the mitochondrion, the NADH can be more efficiently converted to ATP through oxidative phosphorylation. Interestingly, the malate-aspartate (NADH) shuttle of different tumours can contribute between 20 and 80 % of the total respiratory rate [27, 28]. As the mammalian blastocyst has a high respiratory quotient [1] combined with high levels of lactate production [5], it is plausible that glycolysis is used to derive ATP oxidatively through the malate-aspartate shuttle. In support of this it has recently been demonstrated that in the mouse preimplantation embryo malate-aspartate shuttle activity is essential for the regulation of metabolism during the preimplantation period [7]. Furthermore, if the activity of the shuttle is inhibited in the blastocyst, then subsequent implantation and fetal development are significantly reduced [29]. Such data further highlight the significance of maintenance of embryonic metabolism to subsequent viability and normality.
Comparison of Metabolic Activities of Embryos Developed In Vitro Compared to In Vivo
One advantage of working with an animal model, such as the mouse, is the ability to access and analyse embryos developed in vivo, thereby being able to determine how similar, or otherwise, embryos are when maintained in vitro. If a mouse blastocyst is collected from the uterus and its metabolism quantitated immediately, it is observed that just less than 50 % of glucose consumed will be released into the surrounding medium as lactate [5]. It has been established that when developed in simple culture conditions (i.e. a culture medium lacking amino acids and vitamins, and with only glucose, pyruvate and lactate as energy sources, which is typical of the early human embryo culture media such as HTF [30]), resultant blastocysts exhibit a significant increase in lactate production [5, 31] and a concomitant reduction in carbohydrate oxidation [32], which has been documented to be associated with a significant loss of viability [33]. Lane and Gardner [34] demonstrated that placing a mouse blastocyst that had developed in vivo and collected directly from the uterus, into a simple culture medium induced aberrant metabolism within just 3 h of incubation. They went on to show that collecting mouse blastocysts in the presence of amino acids and vitamins greatly reduced this metabolic trauma. Furthermore, when in vivo developed blastocysts were incubated for just 6 h in a simple medium prior to transfer, they exhibited a significant reduction in implantation rate and subsequent fetal development. In contrast, blastocysts incubated in the presence of amino acids and vitamins for the 6 h period were not significantly compromised post-transfer.
Key points arising from the aforementioned study are: that culture-induced metabolic stress, i.e. incubation in a medium lacking amino acids and vitamins, resulted in a rapid loss of metabolic regulation, and that secondly, this metabolic perturbation not only reduced implantation but also impacted fetal development, culminating in lighter foetuses. So an incubation of just a few hours in a stressful environment can induce metabolic changes that have downstream effects leading to compromised fetal development. Hence, several studies have used this model to develop culture conditions which ensure that embryos in vitro exhibit a metabolic profile similar to those embryos developed in vivo [35]. These data firmly establish that quantifying embryo metabolism is an appropriate physiological parameter to relate to subsequent viability after transfer. Furthermore, the above study was performed on in vivo developed blastocysts, and it has been shown that embryos post-compaction are far more resilient to environmental stress than the cleavage stage embryo. Consequently, exposing cleavage stage embryos to stress, i.e. prior to compaction, may have even greater downstream effects [36, 37].
Relationship Between Carbohydrate Metabolism and Embryo Viability
As discussed, substantial perturbations in the relative activity of pathways involved in intermediary metabolism culminate in compromised embryo development in culture and a reduction of viability post-transfer. Data from such studies have been invaluable in the development of modern culture systems that support normal metabolic function in the developing embryo, thereby promoting the development of viable human embryos [38]. A relationship between carbohydrate utilisation by embryos and resultant viability has been established both in animal models [33, 39, 40] and the human [41, 42] (reviewed in detail in Chap. 23). Post-compaction glucose consumption is positively correlated with subsequent viability. Furthermore, should the metabolism of glucose by the blastocyst differ from that expected, i.e. excessive glycolytic activity, then it has been established that viability is significantly compromised in the mouse model. These data support the hypothesis that it is not only the rate at which a nutrient is consumed that reflects developmental potential but also the metabolic fate of the nutrient measured. Of further interest is the observation that female embryos are metabolically more active than males [41, 43]. This finding is consistent with the observed differences in gene expression profiles [44, 45], and subsequent proteome [46], between male and female embryos.
Beyond the Carbohydrate Triad: Analysis of Amino Acid Utilisation
Alternative energy sources for cells, other than those present in typical embryo culture media (i.e. glucose, pyruvate and lactate) include free fatty acids (FFA) and amino acids. Although FFA may have a role in the development embryos of certain species (typically those with a high endogenous lipid content, such as the cow and pig), and their uptake by human embryos has been quantitated [47], further work is required to understand how FFA impact the metabolism of the embryo. Consequently they will not be discussed further here. However, it would be prudent to watch developments in this area, especially with regard to the role of FFA metabolism in oocyte maturation [48]. The beneficial effects of amino acids on embryos in culture have been demonstrated for several years. Amino acids are abundant in oviduct and uterine fluids [49, 50] and are important regulators of embryonic function. As well as their documented effects as biosynthetic precursors [51], buffers of intracellular pH in the embryo [52], antioxidants [53] and in signalling and differentiation [54–56], amino acids also serve as energy sources [23]. Furthermore, our understanding of how embryo energy metabolism is regulated has changed recently with the determination that the malate-aspartate shuttle is involved in the control of carbohydrate metabolism [7]. A paradigm held for over 40 years was that the fertilised oocyte had an absolute requirement for pyruvate [2]. However, recent analysis of embryo physiology has revealed that lactate and aspartate can substitute for pyruvate at the 1-cell stage, the two nutrients presumably generating sufficient energy through the malate-aspartate shuttle to support development.
The relationship between amino acid utilisation and embryo development and viability has been analysed by Leese and colleagues [57–59], and reviewed in detail by Houghton (Chap. 24). Using HPLC to quantitate amino acids in embryo culture medium, it was observed that a different pattern of amino acid utilisation existed between embryos that went on to form a blastocyst compared to those embryos that failed to develop in vitro. It was observed that leucine was taken up from the culture medium more by embryos which went on to develop [57]. The profiles of the amino acids alanine, arginine, asparagine, glutamine and methionine utilisation also correlated with blastocyst formation, although no relationship between amino acid utilisation and blastocyst quality or viability was established. Subsequently, Brison et al. [58] reported that changes in concentration of amino acids in the spent medium of human zygotes cultured for 24 h to the 2-cell stage in an embryo culture medium containing a mixture of amino acids, using HPLC. It was found that asparagine, glycine and leucine were all significantly associated with clinical pregnancy and live birth. Furthermore, and consistent with the data on glucose consumption, there appears to be gender differences with regard to amino acid utilisation [59, 60], consistent with differences in the proteomes of male and female embryos [43].
An important point to note is that all of the studies analysing the utilisation of amino acids by human embryos have been performed in the presence of 20 % oxygen, the significance of which is just now being realised and is discussed below.
Impact of the Culture Environment on Embryonic Metabolism
The relative concentrations of nutrients present in culture media directly affects embryo metabolism [61]. However, other factors of the culture system such as medium pH [62] and the concentration of oxygen used in the incubator can impact metabolic function. Historically, atmospheric oxygen (∼20 %) has been in tissue culture, and human IVF laboratories subsequently adopted this level of oxygen for embryo culture. However, the physiological concentration of oxygen within the female reproductive tract has been reported to be below 10 % [63–65]. Consistent with such data, embryo development in all mammals studied to date is significantly improved by culture in an oxygen concentration of 5–7 %, compared to 20 % [66–69], with a high oxygen concentration having its most detrimental effects at the cleavage stages [70]. Similar data sets are now being documented for the human embryo [71–74].
A significant body of work has been amassed on the effects of oxygen concentration on mammalian embryo development. It transpires that atmospheric oxygen has a significant negative impact on blastocyst gene expression [36, 75], the embryonic proteome [76] and more recently it has been established that oxygen concentration has a significant impact on embryo metabolism, impacting the utilisation of both carbohydrates and amino acids [77] (Fig. 3). Of interest the effects of oxygen on metabolism were stage-specific. During the cleavage stages, 20 % oxygen was associated with an overall increase in amino acid turn over and pyruvate uptake by embryos. In contrast, post-compaction 20 % oxygen was associated with a decrease in amino acid turnover and glucose uptake. Given that it has been established that glucose uptake by the embryo post-compaction is positively correlated with viability, such findings are consistent with reports of lower pregnancy rates following culture in 20 % oxygen, i.e. the decrease in glucose utilisation in the presence of 20 % oxygen is related to the reduction in viability. Given the documented harm atmospheric oxygen imparts on the developing embryo and its physiology, its continued use in human IVF can no longer be condoned.
What Are the Accepted Patterns of Metabolic Activity? Quietness Revisited
A novel hypothesis proposed by Leese [78] suggested that those embryos with a less active, or a ‘quiet’ metabolism are those that go on to give rise to pregnancies. Data to support this hypothesis have been obtained from different species and reviewed recently [79]. In essence it was proposed that embryos with a low metabolic activity reflected a less stressed physiology, and consequently those embryos classified as viable would be those that had low nutrient uptake and turn over [79]. However, a growing number of recent studies have generated data that do not provide support for this hypothesis [33, 41, 80, 81], rather they indicate that viability is associated with increased metabolic activity. So how can this apparent paradox be resolved? An analysis of the studies upon which the ‘quiet hypothesis’ was built reveals that a common factor among them is the use of 20 % oxygen, either for embryo culture and analysis, or during the actual analysis of metabolism. Given the documented negative impact of 20 % oxygen on embryonic gene expression, proteome and metabolism described above, the significance of the ‘quiet hypothesis’ for embryos cultured under physiological oxygen conditions must be carefully reviewed and studies performed to determine what is the optimal nutrient utilisation profile under physiological conditions.
We know that when mammalian embryos are stressed, especially by 20 % oxygen, their metabolic profile changes significantly [77]. Data to date [79] indicate that those embryos cultured in 20 % oxygen which are able to develop do have a lower turnover of nutrients. One explanation for this finding may be related to the inability of unhealthy or developmentally compromised embryos to tolerate oxidative stress. Suboptimal embryos may have lower endowments of antioxidants or less robust systems for responding to oxidising agents and consequently may have to resort to other energetically consuming mechanisms to preserve their redox status. In this context, the quiet embryo hypothesis would pertain more to the embryo’s ability to respond to oxidative stress than to its innate metabolic characteristics under physiologic conditions. Studies evaluating the metabolism of the same embryos in both low and high oxygen conditions could test this hypothesis. Thus far, little attention has been devoted to the development and application of stress tests, i.e., exposing embryos to safe, well-controlled stresses and monitoring their responses, to evaluate embryonic health and developmental potential. There are certainly many clinical situations in which stress tests are used to diagnose disease or disease susceptibility such as exercise stress tests for cardiac disease and glucose tolerance tests for gestational diabetes. The challenge for the development of an embryonic stress test is to ensure that the stressor does not cause lasting harm. As mentioned previously, exposure to high oxygen tension is not an acceptable stressor based on our understanding of the short- and long-term effects of this exposure.
It is evident that when a 5 % oxygen concentration is used during culture, the metabolism of embryos on day 4 and 5 at the morula and blastocyst stages, is significantly higher in those embryos that give rise to pregnancies, compared to those embryos that fail to develop post-transfer. Such data, therefore, do not support the ‘quiet hypothesis’ for embryos beyond the 8-cell stage. In contrast, the data obtained for day 4 and 5 human embryos and their subsequent pregnancy outcome, were collected under low oxygen conditions. These data that reveal those embryos with the more active metabolism give rise to pregnancies. Interestingly, recent data on oxygen consumption by cleavage stage human embryos indicates that at the cleavage stage viability is associated with an increased oxygen consumption rate, reflecting an increase in respiration rate [81]. At present, we do not know if there is a value that is too high to be consistent with viability, although it is most plausible. There will likely be upper and lower values of metabolic normality, outside which embryo will show a decline in viability. Given that there is an overall increase in most metabolic activities as the preimplantation embryo proceeds through development, it would seem more likely for compromised embryos to have lower rather than higher metabolic activities relative to their healthy cohorts. Furthermore, as glucose uptake [41, 43] and amino acid utilisation [59] are related to the sex of embryo, such data need to be factored in to further hypotheses on embryo metabolism and optimal rates of metabolic activity for embryo selection.
Conclusions
Some 30 years after Renard et al. showed a relationship between glucose uptake by day 10 cow blastocysts and subsequent pregnancy outcome [39], data sets relating the patterns of nutrient utilisation by the human embryo with pregnancy outcome, are growing [41, 58, 59]. Further analysis of human embryo physiology will assist in establishing the metabolic algorithms for embryo selection. Growing data indicate that stage-specific and sex-specific differences in metabolism exist during the preimplantation period. Understanding the nature of these differences, the impact of culture conditions (such as medium composition and the concentration of oxygen used) has important implications for embryo selection. As development proceeds, there is an increase in asynchrony amongst embryos of a cohort and this will be manifest as an increased variation in nutrient utilisation (Fig. 4). Consequently it is predicted that differences in embryo metabolism will be more readily detected with increasing developmental stage.
It is also evident that changes in metabolic activity not only affect subsequent implantation potential, but have downstream effects through to fetal development and beyond. Given that perturbations in metabolic activity are linked to epigenetic regulation of cell function [82], assessment of a viable metabolism should facilitate the creation of conditions that support the genetic stability and epigenetic integrity of the embryo.
Whereas the technologies for such work currently resides in a handful of laboratories around the world, the rapid developments in laboratory-on-a-chip technologies may well ensure that the assays required will be able to be housed in microfluidic devices and subsequently such analyses will be readily available to the general IVF community [83, 84].
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The author is indebted to Dr Mark Johnson for his comments on the manuscript.
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Gardner, D.K. (2013). Metabolism of the Viable Human Embryo. In: Gardner, D., Sakkas, D., Seli, E., Wells, D. (eds) Human Gametes and Preimplantation Embryos. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6651-2_20
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