Abstract
Agrobacterium tumefaciens transforms a wide variety of dicotyledonous plants by introducing a specific piece of DNA (T-DNA) of a large tumor-inducing plasmid (Ti-plasmid) into plant cells. A wide variety of dicotyledonous plants and some gymnosperms are susceptible to infection. However, the ease of infectability and the efficiency of transformation vary widely within these groups. As a general rule, most of the gymnosperms are infected only with difficulty if at all. The transfer of the T-DNA to plant cells depends upon the activity of a region of the Ti-plasmid termed the virulence (vir) region. We have shown that the efficiency of transfer and the host range properties of Agrobacterium are, in large part, due to the genes in the vir region. In particular, virA, a regulatory molecule which recognizes plant signals, seems to be especially important. In addition, we have identified other regions which seem to be associated with the efficiency of transformation.
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© 1988 Plenum Press, New York
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Nester, E.W. (1988). Analysis of Host Range in Transformation of Higher Plants by Agrobacterium Tumefaciens . In: Hanover, J.W., Keathley, D.E., Wilson, C.M., Kuny, G. (eds) Genetic Manipulation of Woody Plants. Basic Life Sciences, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1661-9_13
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DOI: https://doi.org/10.1007/978-1-4613-1661-9_13
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