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Plantlet Regeneration in vitro via Adventitious Buds and Somatic Embryos in Norway Spruce (Picea Abies)

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Genetic Manipulation of Woody Plants

Part of the book series: Basic Life Sciences ((BLSC,volume 44))

Abstract

Methods are described for plant regeneration via adventitious bud formation and somatic embryogenesis from mature zygotic embryos of Picea abies. Adventitious buds are formed on embryos pulse-treated with cytokinin. A few epidermal and subepidermal cells adjacent to the stomata are stimulated to give rise to meristemoids which subsequently develop further into adventitious buds. Embryogenic callus is formed when the embryos are cultured on medium containing both auxin and cytokinin. The somatic embryos arise from the region between the hypocotyl and the cotyledons. The different developmental stages during initiation and development of adventitious buds and somatic embryos are described.

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Author information

Authors and Affiliations

  1. Department of Plant Physiology, University of Uppsala, S-751 21, Uppsala, Sweden

    Sara von Arnold & Inger Hakman

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  1. Sara von Arnold
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  2. Inger Hakman
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Editors and Affiliations

  1. Michigan State University, East Lansing, Michigan, USA

    James W. Hanover  & Daniel E. Keathley  & 

  2. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Claire M. Wilson  & Gregory Kuny  & 

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© 1988 Plenum Press, New York

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von Arnold, S., Hakman, I. (1988). Plantlet Regeneration in vitro via Adventitious Buds and Somatic Embryos in Norway Spruce (Picea Abies). In: Hanover, J.W., Keathley, D.E., Wilson, C.M., Kuny, G. (eds) Genetic Manipulation of Woody Plants. Basic Life Sciences, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1661-9_12

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  • DOI: https://doi.org/10.1007/978-1-4613-1661-9_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8922-7

  • Online ISBN: 978-1-4613-1661-9

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