Abstract
Candida kefyr DSM 70106 was cultivated in a medium containing inulin as a carbon source. About 92% of the inulinase was recovered directly from the medium. Different concentration (Cf) and enrichment (Ef) factors were obtained, using the following methods: Cross-flow filtration (microfiltration and cell diafiltration were carried out using a rotary filter; enzyme ultrafiltration and diafiltration were performed using a cassette module): Cf = 7.5 and Ef = 2.2; liquid-liquid extraction of N-Benzyl-N-Dodecyl-N-bis[2-hydroxyethyl] ammonium chloride (BDBAC) reversed micelles: Cf = 2.5 and Ef = 2.7; and expanded-bed adsorption: Cf = 2.8 and Ef = 4.3.
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References
Kierstan, M. (1980), Process Biochem. 15, 2–4.
Kaur, N., Kaur, M., Gupta, A. K., and Singh, R. (1992), J. Chem. Technol. Biotechnol. 53, 279–284.
Hauly, M. C. O., Bracht, A., Beck, R., and Fontana, J. D. (1992), AppL Biochem. Biotechnol. 34/35, 297–308.
Manzoni, M., and Cavazzoni, V. (1992), J. Chem. Technol. Biotechnol. 54, 311–315.
Claessens, G., Van Laere, A., and De Proft, M. (1990), J. Plant Physiol. 136, 35–39.
Manzoni, M., and Cavazzoni, V. (1988), Lebensm. Wiss. Technol. 21, 271–274.
Pessoa Jr., A; Vitolo, M., and Hustedt, H. (1996c) Appl. Biotechnol. Biochem. 57/58, 699–709.
Parekh, S., and Margaritis, A. (1986), Agric. Biol. Chem. 50, 1085–1087.
Krei, G., Meyer, U., Börner, B., and Hustedt, H. (1994), Bioseparation 5, 175–183.
Luisi, P. L., Giomini, M., Pileni, M. P., and Robinson, B. H. (1988), Biochim. Biophys. Acta 947, 209–246.
Krei, G. A. and Hustedt, H. (1989), BioEngineering 3/4, p.32–41.
Frej, A.-K., B., Hjorth, R., and Hammarström, A. (1994), Biotechnol. Bioeng. 44, 922–929.
Bradford, M. A. (1976), Anal Biochem. 72, 248–254.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951), J. Biol. Chem. 193, 265–275.
Kroner, K. H. and Schütte, H. (1989), in International Technical Conference on Membrane Separation Processes pp. 279–290.
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Pessoa, A., Vitolo, M. (1998). Downstream Processing of Inulinase Comparison of Different Techniques. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_46
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DOI: https://doi.org/10.1007/978-1-4612-1814-2_46
Publisher Name: Humana Press, Totowa, NJ
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