Abstract
The saccharification of microcrystalline cellulose by reconstituted ternary mixtures of purified cellulases (one endoglucanase and two cello-biohydrolases) has been studied over the entire range of mixture compositions. Ternary plots are used to compare the performance of five synthetic mixtures drawn from the cellulase systems of Acidothermus cel-lulolyticus, Trichoderma reesei, Thermomonospora fusca, and Thermotoga nea-politana. Results reveal that at least one synthetic mixture utilizing enzymes from three different organisms delivers performance competitive with that of a “native” (i.e., co-evolved) ternary system drawn exclusively from T. reesei. This heterologous system, consisting of the endoglucanase El from A. cellulolyticus and the exoglucanases CBHI from T. ressei and E3 from T. fusca, is forgiving from the system-design point of view, in that it delivers high saccharification rates over a wide range of mixture compositions.
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Baker, J.O., Ehrman, C.I., Adney, W.S., Thomas, S.R., Himmel, M.E. (1998). Hydrolysis of Cellulose Using Ternary Mixtures of Purified Cellulases. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_37
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DOI: https://doi.org/10.1007/978-1-4612-1814-2_37
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