Abstract
The use of anticoagulant therapy plays an important role particularly in the early management of patients with acute coronary syndrome (ACS) and in those undergoing percutaneous coronary interventions (PCI). These include a variety of agents available for parenteral administration which may be chosen depending on the level of ischemic and hemorrhagic risk of the patient as well as the planned early management (conservative vs. invasive). Indeed, not only the choice of therapy by also timing of administration and cessation are key determinant of short and long-term outcomes of anticoagulant therapies in the setting of ACS and PCI. Given that ACS patients persist with elevated thrombin levels following an ACS event, there also has been an emerging interest on adding oral anticoagulant therapy to standard antiplatelet regiment for long-term secondary prevention of ischemic event. The present chapter is an overview of the different options of anticoagulant therapy that are available for use in ACS and PCI patients. In particular, the basic principles of pharmacology, rationale for use, indications, contraindications, dosing considerations and side effects of currently available anticoagulant therapies are summarized and recent advances in the field provided.
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Keywords
- Percutaneous Coronary Intervention
- Acute Coronary Syndrome
- Major Bleeding
- Stent Thrombosis
- Acute Coronary Syndrome Patient
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Introduction and General Considerations
Atherosclerotic disease is a progressive and diffuse disease which may affect any arterial vasculature [1]. Coronary artery disease (CAD) starts in the late teens under the form of fatty streaks and progress with age, until atherosclerotic plaques are developed. Thrombotic complications of atherosclerotic plaques occur mostly following rupture, fissure or superficial endothelial cell erosion [2]. This leads to activation of the platelet cascade, characterized by adhesion, activation, and aggregation, as well as activation of the extrinsic pathway of the coagulation cascade which ultimately results in thrombin generation. Thrombus formation can be subclinical, and thus favor plaque progression, or clinical, and thus leading a clinical manifestation of an acute coronary syndrome (ACS) [1, 2]. Nonocclusive thrombi typically lead to a non-ST elevation ACS (NSTE-ACS) and occlusive thrombi to a ST-elevation myocardial infarction (STEMI) [1–3]. The absence or presence of cardiac biomarkers allows to differentiate NSTE-ACS patients into two categories: unstable angina (UA) or non-ST elevation myocardial infarction (NSTEMI). In the setting of percutaneous coronary interventions (PCI), plaque rupture is iatrogenically induced. Therefore, these patients are also exposed to the risk of intraprocedural thrombotic complications which warrant specific therapies.
The extensive procoagulant and prothrombotic actions of thrombin underlie its key role in the setting of ACS, given that it represents the last step of coagulation as it converts fibrinogen to clottable fibrin by releasing fibrinopeptides A and B [4]. Moreover, thrombin is also the most potent naturally occurring platelet agonist and therefore has been considered as a pharmacological target in order to prevent the formation of fibrin- and platelet-rich thrombi induced by thrombin. The mechanism of generation of acute platelet-rich thrombus and promoting vascular healing after arterial injury has been broadly described [5–10]. On the platelet surface, thrombin binds to its specific receptor on platelets ultimately leading to the expression of activated glycoprotein IIb/IIIa (GP IIb/IIIa) receptors. Via the GP IIb/IIIa receptor platelets are cross-link activated by ligands such as fibrinogen forming platelet aggregates. Moreover such platelet aggregates increase the surface area for the prothrombinase complex by providing a phospholipid membrane platform on which a complex of activated factors V and X and calcium ions can form contributing to thrombus formation [7] and amplifying thrombin generation. Thrombin becomes resistant to inactivation by the heparin/antithrombin complex when bound to fibrin, fibrin degradation products or subendothelial matrix [8–10].
Given that this central role of thrombin in arterial thrombogenesis, therapeutic agents have been developed to target either thrombin or modulators of thrombin generation within the coagulation cascade (Fig. 3.1). These include a variety of agents available for parenteral administration, used in combination with antiplatelet therapies (discussed in details elsewhere in this book), used in ACS and PCI setting which may be chosen depending on the level of ischemic and hemorrhagic risk of the patient as well as their planned early management (conservative vs. invasive). Indeed, not only the choice of therapy by also timing of administration and cessation are key determinant of short and long-term outcomes. Given that ACS patients persist with elevated thrombin levels following an ACS event, there also has been an emerging interest on adding oral anticoagulant therapy to standard antiplatelet regiment for long-term secondary prevention of ischemic event. In this chapter, we provide an overview of the basic principles of pharmacology, rationale for use, indications, contraindications, dosing considerations and side effects of currently available anticoagulant therapies are summarized and recent advances in the field provided.
Anticoagulant Therapy: Classification
The classification of anticoagulant agents is based primarily on the target coagulation enzyme which is inhibited. Most clinically available anticoagulant therapies block factor (F) II or thrombin and FX. Each class of inhibitors blocking a specific target can then be classified according to its mechanism of action to exert its inhibitory effects as direct or indirect, based on the need of a co-factor without which these agents would provide minimal or null effects (Fig. 3.2). Finally, anticoagulant therapies can be classified according to their route of administration (parenteral or oral).In the setting of ACS and PCI, parenteral agents are used and include a variety of thrombin inhibitors (direct and indirect). Anti-X inhibitors are also used in ACS patients, mostly medically managed, although to a lesser extent than thrombin inhibitors and currently only an indirect anti-X inhibitor for parenteral use is clinically available. These agents are described in details below.
Thrombin Inhibitors
Thrombin inhibitors can be classified into two broad categories according to the presence or absence of a plasma cofactor needed to exert its effects: indirect and direct thrombin inhibitors (DTIs). Details of these agents are described below.
Indirect Thrombin Inhibitors
Indirect thrombin inhibitors include unfractionated heparin (UFH) and low-molecular weight heparin (LMWH), which in the absence of the cofactor antithrombin (AT), an endogenous inhibitor of several activated clotting factors have minimal or no intrinsic anticoagulant activity.
Unfractionated Heparin
Mechanism of Action and Pharmacokinetic/Pharmacodynamic Profile
UFH is a highly sulfated glycosaminoglycan composed by a heterogeneous mixture of variable molecular weight polysaccharide molecules. The structure and the mechanism of action of heparin are described in Fig. 3.3. UFH is administrated through anintravenous (IV) or subcutaneous (SC) route because its polysaccharide chain is degraded in gastric acid. UFH should not be administrated intramuscularly because of the danger of hematoma generation. UFH is metabolized primarily by the liver (by heparinase to uroheparin, which has only small AT activity) and partially metabolized by the reticuloendothelial system [13].
After administering an IV bolus followed by continuous IV infusion, an immediate anticoagulant effect can be achieved, which underscores this route of administration in patients with ACS. SC administrations (10 % lower bioavailability than IV) delay 1 hour (h) the anticoagulant effect of UFH, achieving the peak plasma levels at 3 h [14, 15]. After entering in the bloodstream, the binding of UFH to a number of plasma proteins, endothelial cells, macrophages, and von Willebrand factor (vWF), takes places, reducing its anticoagulant activity, leading to heparin resistance, and inhibiting vWF-dependent platelet function [16–19] The variability of anticoagulant response, particularly in patients with thromboembolic disorders, and its complex pharmacokinetic (PK) profile, is caused by the heterogeneity of UFH properties [16]. Clearance of UFH takes place by a saturable depolymerization process produced by the binding of UFH to endothelial cells and macrophages [20, 21]. A non saturable renal clearance, a slower elimination pathway, occurs mostly with supraclinical doses of UFH [22].
The PK profile of UFH explains why the intensity and duration of anticoagulant response results nonlinear to therapeutic doses of UFH and increases excessively with cumulative dose. In line with this, an IV bolus of 100 U/kg increases the biological half-life of UFH from 30 min (after an IV bolus of 25 U/kg) to 60 min, and a bolus of 400 U/kg for 30–150 min [14, 22–24]. The anticoagulant effect of UFH lasts just a few hours after cessation of UFH due to the fast clearance. In fact, it is possible to reactivate the coagulation process and because of an increase of thrombin activity after cessation a phenomenon called “heparin rebound” may occur, despite concomitant aspirin treatment [25, 26]. Changing from IV to SC dosing before interruption of UFH could mitigate this effect [27, 28].
The heterogeneous binding properties to several cells and proteins confer UFH other biologic effects in addition to its anticoagulant effects, such as alteration of platelet function, an increase in vessel wall permeability, a suppression of smooth muscle cell proliferation. Inhibition of osteoblast formation and osteoclast activation has also been described by in vitro studies of UFH [28–35]. Clinically the most relevant non anticoagulant effect of UFH is its potential to induce immune‐mediated platelet activation known as heparin‐induced thrombocytopenia (HIT) [24, 36, 37].In comparison with LMWH,UFH causes more frequently thrombocytopenia (defined as a platelet count <100,000/μL or a 50 % reduction in baseline platelet count), with an approximate incidence of 0.3 %. The phenomenon of inadequate response to UFH is described by heparin resistance, demanding higher than usual doses of UFH to acquire the desired anticoagulant effect. More rapid clearance of UFH [38], increased heparin-binding proteins [17], AT deficiency, or increased factor VIII levels [39] can explain this phenomenon.
Indications
The benefit of UFH in the setting of ACS, particularly UA/NSTEMI, has been extensively demonstrated in many trials establishing it as a class IA therapy concomitantly with platelet inhibitors [40, 41]. Metaanalyses of six relatively small randomized, placebo-controlled trials with UFH for treatment of UA/NSTEMI have led to guideline recommendations for UFH use in ACS [42–48] (Table 3.1).
For STEMI patients undergoing PCI the use bivalirudin with or without prior treatment with UFH is considered preferable to UFH andglycoprotein IIb/IIIa inhibitors (GPI) [49, 50] (Table 3.2). However, during PCI or combined with fibrinolytic therapy in the setting of STEMI adjunctive anticoagulation with UFH has been also demonstrated in clinical trials, but the use of SC or IV UFH as an adjunct to streptokinase (SK) remains controversial. UFH is recommended in high-thrombotic-risk patients SK-treated [49, 50].
Dosages, Monitoring and Reversal
Based on evidence-based guideline recommendations and available clinical trial data [49, 50], initial dosing of UFH in patients with ACS should start with an IV weight-based bolus followed by continuous IV infusion [40]. At each institution, because of the laboratory variation, nomograms should be performed in order to reach activated partial thromboplastin time (aPTT) values in the target range, for many aPTT reagents [40, 49]. In a patient with ACS, UFH is usually initiated at time of clinical presentation. For UA/NSTEMI, an initial IV bolus of 60–70 U/kg (maximum 4,000 U) followed by continuous infusion of 12–15 U/kg/h (maximum 1,000 U/h is recommended) [40, 49]. For STEMI patients on non-SK fibrinolytic therapy regimens, the dosing of UFH is at the lower end of this range (Table 3.1) [51]. Given that higher aPTT responses to UFH have been related with older age, low body weight, and female sex, these factors should be particularly considered in dosing decisions. Also smoking and diabetes should be considered because have been associated with an attenuated response to UFH [52, 53]. Higher than usual doses of UFH to obtain the desired anticoagulant effect are needed when the phenomenon of inadequate response to UFH occurs because of a heparin resistance [17]. Higher doses of UFH, [54, 55] prolonged or repeated exposure, [56] and concomitant use of fibrinolytic [57–59] or GPI [60] have been associated with an increase of the hemorrhagic and non-hemorrhagic complications. During UFH infusion platelet count and hemoglobin should be measured at least once a day. More than a 20 % of patients could have a mild, clinically insignificant drop in their platelet count (above 100,000/μL). All UFH therapy (included IV flush) should be immediately interrupted if there is a drop in platelet count (suspecting HIT). Moreover a screening for anti–platelet factor-4 antibodies, followed by the more definitive serotonin release assay, is also required [56, 61, 62]. Reversal of UFH effects can be achieved with protamine, a small arginine-rich (e.g. cationic) nuclear protein purified from fish sperm. An IV bolus of 1 mg of protamine could be used to rapidly neutralize 100 U of UFH, reversing its anticoagulant effect [63, 64].
In the setting of PCI, the recommended dosage of IV UFH depends on prior exposure to anticoagulant therapy [65] (Table 3.2). In patients who have received prior anticoagulant therapy, if IV GPI are planned, UFH should be added as needed (e.g., 2,000–5,000 U) to achieve an ACT of 200–250 s. If IV GPI are not planned, the additional UFH should aim to achievean ACT of 250–300 s for HemoTec, 300–350 s for Hemochron. Without prior anticoagulant therapy, if IV GPI are planned, a 50–70 U/kg bolus should be administrated to achieve an ACT of 200–250 s. However, if IV GPI are not planned, the bolus dose may be 70–100 U/kg to achieve target ACT of 250–300 s for HemoTec, 300–350 s for Hemochron. Full-dose anticoagulation is no longer used after successful PCI procedures [65].
Side Effects and Contraindications
Bleeding is the main side effect related with the use of IV UFH, but recent trials demonstrated that this is <3 % [66]. However, higher heparin dosages, concomitant use of antiplatelet drugs or oral anticoagulant, and increasing age (>70 years) increases the bleeding risk [66]. As stated above, the development of HIT (usually between 5 and 15 days after the initiation of UFH) is another important problem associated with heparin therapy. Patients who have a previous exposition to heparin may have a more rapid onset [36, 37, 61].An alternative antithrombin drug must be chosen in the setting of HIT. The development of osteoporosis and rare allergic reactions less commonly have been described with long-term use of heparin.
Low-Molecular-Weight Heparin
Mechanism of Action and Pharmacokinetic/Pharmacodynamic Profile
Because of the many known limitations associated with the use of UFH (nonspecific binding, the production of antiheparin antibodies that may induce thrombocytopenia, continuous IV infusion, and the necessity for frequent monitoring), LMWHs, potent inhibitors of both thrombin (anti-IIa effects) and FXa have been developed [55]. These heparins do not require monitoring due to their more rapid and predictable absorption, anticoagulant response and greater than 90 % bioavailability [67]. Anti‐Xa levels peak 3–5 h after a SC dose of LMWH [68, 69]. Fewer platelet agonist effects less often associated with HIT also characterize LMWHs. After 3–6 h following a SC dose a renal elimination (largely dose‐independent) takes place, leading to prolonged anti‐Xa effect and linear accumulation of anti‐Xa activity in patients with a creatinine clearance (CrCl) <30 ml/min [70–72]. The anticoagulant effect of LMWHs can be measured via anti-FXa levels, with a target peak anti-Xa level 0.6–1.0 U/mL derived from studies of venous thromboembolism treatment [73, 74].
Depolymerization of the polysaccharide chains of UFH originates LMWHs, producing fragments ranging from 2,000 to 10,000 Da [68, 69, 75]. The unique pentasaccharide sequence needed to bind to AT is contained by these shorter chain lengths, that are too short (<18 saccharides) to form the ternary complex crosslinking AT and thrombin. Consequently, the primary effect of LMWHs is limited to AT‐dependent FXa inhibition. LMWHs result in a FXa:thrombin inhibition ratio ranging from 2 to 4:1, in comparison to UFH where the ratio of FXa:thrombin inhibition is 1:1 [76]. Also compared to UFH, LMWHs have a more favorable and predictable pharmacokinetic profile because of its reduced binding to plasma proteins and cells [68].
Enoxaparin is the most studied and developed LMWH preparation in clinical trials of UA/NSTEMI, STEMI and PCI. The main difference among LMWHs is their molecular weight and therefore the relative anti‐Xa:anti IIa ratio. Enoxaparin has a mean molecular weight of 4,200 Da with anti‐Xa:anti‐IIa ratio of 3.8; dalteparin with a mean molecular weight of 6,000 Da has an anti‐Xa: anti‐IIa ratio of 2.7 [69]. The preferential binding ratio to FXa over thrombin, less plasma protein binding, attenuated platelet activation, lower risk of HIT, and reduced binding to osteoblasts are the theoretical pharmacologic advantages of LMWH over UFH [35, 69, 77–79].
In the majority of clinical settings routine monitoring of anti-FXa levels is not necessary due to the predictable anticoagulant response to LMWHs. The Thrombolysis in Myocardial Infarction 11A (TIMI 11A) trial assessed the PK and pharmacodynamic (PD) properties of enoxaparin including an enoxaparin clearance of 0.733 L/h, a distribution volume of 5.24 L, and an elimination half-life of 5 h [80]. Enoxaparin clearance modeled and predicted hemorrhagic complications and was significantly related to patient weight and CrCl. A 27 % decrease in enoxaparin clearance was correlated with CrCl <30 mL/min, causing a 3.8-fold increased risk of major hemorrhage [81].In patients with CrCl <30 mL/min, the dose of enoxaparin needs to be adjusted and reduced in half (e.g. 1 mg/kg/day). Age, female sex, lower body weight, reduced renal function, and interventional procedures can increase the risk of bleeding with LMWH which is highly dose-related [80].
The probability of LMWHs to origin anti–platelet factor-4 antibody formation is about three times lower than UFH [56, 82] and produces less frequently HIT in patients who have anti-platelet factor-4 antibodies, although LMWHs are similarly reactive as UFH in activation assays of washed platelets using serum from HIT patients [56, 83]. Also osteopenic effect is lower compared with UFH [84]. Protamine sulfate (1 mg per each 1 mg of enoxaparin within 8 h) may be used to neutralize the anti-IIa effect of LMWH when hemorrhagic complications, but it is variable and uncertain the grade to which the anti-Xa activity of LMWH is neutralized by protamine [85].
Indications
The safety and efficacy of LMWHs has been demonstrated in patients with UA/NSTEMI and STEMI, and also in patients undergoing PCI [40, 49, 86]. LMWH with UFH have been directly compared in 9 randomized trials [87–95] in UA/NSTEMI patients. No significant differences in death or MI were observed in patients treated with LMWH compared with UFH in two studies using dalteparin [87, 88] and one with nadroparin [89]. Enoxaparin’s greater anti-Xa-to-anti-IIa ratio when compared with dalteparin, and the extension of its antithrombotic actions to include inhibition of platelet aggregation by blocking the release of vWF could be the reasons of the greater severity of the disease in the patients enrolled in the reported studies [96].
A meta-analysis by Petersen et al. [97] that pooled the data from six trials evaluating 21,946UA/NSTEMI patients randomized to enoxaparin or UFH, showed significant reductions in the combined endpoint of death/MI by 30 days favoring enoxaparin over UFH, particularly in patients who had not received any antithrombin before randomization [97]. There were no significant differences in major bleeding or blood transfusion within the first week of therapy.
The largest and most recent trial comparing enoxaparin to UFH, randomized 10,027 high‐risk patients with UA/NSTEMI undergoing an early invasive strategy using guideline recommended aspirin, clopidogrel and GPI [90]. The primary composite end point of death or MI at 30 days, was no different between enoxaparin and UFH (14 % vs. 14.5 %; OR 0.96; 95 % CI, 0.86–1.06). Enoxaparin compared to UFH produced significantly higher TIMI major bleeding (P = 0.008). Anticoagulant switching effects due to pre‐randomization anticoagulant use and time- rather than ACT-guided sheath removal in the enoxaparin arm were potential causes of the increased bleeding with enoxaparin [90]. Enoxaparin, but not the other LMWHs, is preferred over UFH for the medical management of UA/NSTEMI in the ACC/AHA guidelines [40]. The greatest benefit is described in patients with elevated troponin values.
As adjunctive pharmacotherapy for STEMI patients receiving fibrinolytic therapy, the safety and efficacy of enoxaparin versus UFH has been assessed in 2 trials. Added to fibrinolytic therapy, enoxaparin diminished the risk of in‐hospital reinfarction or refractory ischemia compared to UFH, but at the expense of an increased the rate of intracranial hemorrhage (ICH) among patients over the age of 75 [49, 98, 99].
STEMI patients receiving thrombolytic therapy to enoxaparin or UFH for at least 48 h were randomized in the Enoxaparin and Thrombolysis Reperfusion for Acute Myocardial Infarction-TIMI 25 (ExTRACT‐TIMI 25) trial (n = 20,506) [51]. Patients with impaired renal function defined (CrCl <30 mL/min) received a reduced dose of enoxaparin (1 mg/kg SC q.d.) and also patients older than 75 did not receive bolus of enoxaparin and receive a lower SC dose of 0.75 mg/kg b.i.d). The risk of death and reinfarction at 30 days associated with enoxaparin compared with UFH was significantly reduced (p = 0.001). Enoxaparin was associated with a significant increase of TIMI major bleeding compared with UFH (2.1 % vs. 1.4 %; P < 0.001). In conclusion, the type of fibrinolytic agent and the age of the patient did not affect the net clinical benefit (absence of death, non‐fatal infarction, or ICH) which favored enoxaparin (10.1 % vs. 12.2 %; P < 0.001) [100, 101].
Dosages
UA/NSTEMI patients should receive anticoagulant therapy in addition to antiplatelet therapy as soon as possible after clinical presentation [40, 49] (Table 3.1). Enoxaparin (1 mg/kg SC b.i.d) has demonstrated to be effective for patients in whom an invasive or conservative strategy is selected. As previously mentioned, it is particularly important to adjust the dose of enoxaparin in patients with renal insufficiency (CrCl <30 mL/min), reducing it to 1.0 mg/kg SC daily. LMWH should be continued without loading of UFH if it has been started upstream.
Numerous routes of administration of LMWH can be used in the setting of a PCI: (a) the first dosing regimen option is 1 mg/kg SC b.i.d.; the last dose of SC LMWH has to be administered within 8 h of the procedure and it is also important to warrant that at least 2 SC doses of LMWH are given before the procedure to ensure balanced state; (b) a 0.3 mg/kg bolus of IV enoxaparin is recommended at the time of PCI if the last dose of enoxaparin was given 8–12 h before PCI, (c) another dosing regimen option at the time of PCI is 1 mg/kg enoxaparin IV (if no GPIis used) or 0.75 mg/kg (if a GPI is used) [40, 49]. The STEEPLE (SafeTy and Efficacy of Enoxaparin in PCI patients, an internationaL randomized Evaluation) study found safe the IV dose of 0.5 mg/kg for elective PCI [102].
In the setting of patients with STEMI treated with fibrinolysis if renal function is preserved (<2.5 mg/dL [220 μmol/L] in male patients and <2.0 mg/dL [175 μmol/L] in female patients), we recommend the use of enoxaparin over UFH, continued up to 8 days (Class IIa, LOE B). The recommended dosing for enoxaparin depends on the age: 30‐mg IV bolus followed by 1 mg/kg SC q12 h (maximum of 100 mg for the first two SC doses) for <75 years; and no IV bolus, 0.75 mg/kg SC q12 h (maximum of 75 mg for the first two SC doses) for age >75 years. Enoxaparin should be given before fibrinolytic administration. The continuation of enoxaparin therapy after discharge has not be demonstrated to be beneficial [49], and for this reason enoxaparin regimen uses to be maintained during hospitalization or until day 8 (whatever come first). Regardless of age, if the CrCl is <30 mL/min the dosage of 1 mg/kg subcutaneously every 24 h may be used.
For patients with STEMI undergoing PCI after receiving fibrinolytic therapy with enoxaparin, if the last SC dose was given within the prior 8 h, no additional enoxaparin should be administered; if the last SC dose was given between 8 and 12 h earlier, enoxaparin 0.3 mg/kg IV should be administered (Class I, LOE B) (Table 3.2). These recommendations are based on the analysis of patients who underwent PCI in the ExTRACT TIMI 25 trial [103] as well as on the Enoxaparin as adjunctive antithrombin therapy for ST-elevation myocardial infarction: results of the ENTIRE-TIMI 23 trial [104]. This trial demonstrated that enoxaparin was associated with similar TIMI 3 flow rates as UFH at an early time point, with similar risk of major hemorrhage and greater benefit over UFH regarding to ischemic events through 30 days (P = 0.005) [104].
Side Effects and Contraindications
Patients with contraindications to anticoagulant therapy such as active bleeding, significant thrombocytopenia, recent neurosurgery, ICH, or ocular surgery should not receive LMWH. Patients with bleeding diathesis, brain metastases, recent major trauma, endocarditis, and severe hypertension required to be treated with particular caution. Less major bleeding compared with UFH has been associated with enoxaparin in acute venous thromboembolism. In the setting of ACS, neither UFH nor LMWH are associated with an increase in major bleeding, but in ischemic stroke the both agents are associated with an increase in major bleeding [105]. Hemorrhagic complications could occur due to LMWH, particularly in patients with renal dysfunction (who should receive an adjusted dose of enoxaparin). To neutralize the anti‐IIa effect of LMWH, protamine sulfate may be administered, although the degree of neutralization of the anti‐Xa activity of LMWH is variable and uncertain. In patients with documented or suspected HIT,LMWH are not recommended for use because they can induce it.
Direct Thrombin Inhibitors
The direct thrombin inhibitors (DTIs) bind to thrombin and inhibit its capacity to transform fibrinogen to fibrin, to intensify its own generation through activation of FV, FVIII, and FIX, and to function as a potent platelet agonist [106]. Importantly, DTIs not only block free thrombin, but also inhibit thrombin bound to fibrin in contrast to indirect thrombin inhibitors [12, 107–109] (Fig. 3.3). These properties are indeed important and provide a rationale for their clinical use in the setting of ACS and PCI. The anticoagulant effect of DTIs in healthy volunteers can be reversed by recombinant factor VIIa, although the short half-life of these agents normally avoids the necessity for active reversal [110]. Three DTI’s are approved for clinical use (lepirudin, argatroban, and bivalirudin) and described below.
Hirudin (lepirudin)
Mechanism of Action and Pharmacokinetic/Pharmacodynamic Profile
Hirudin is among the most potent of the natural thrombin inhibitors. It is a 65-amino acid polypeptide located in the salivary glands of the leech Hirudo medicinalis. Lepirudin is a recombinant form of hirudin that irreversibly inhibits thrombin [111]. Several biochemical and molecular biological techniques have been used to study the specific nature of the hirudin-thrombin interaction. The thrombin time (TT) and the aPTT are the most frequently used measures for the anticoagulant activity of hirudin. Bleeding time is not significantly altered by hirudin which does not have direct effects on platelet aggregation or secretion.
Indications
In UA/NSTEMI a single dose of UFH with a single dose of hirudin were compared in both the TIMI-9 and the Global Utilization of Strategies to Open Occluded Coronary Arteries (GUSTO) II trials [57, 58]. Both trials used high doses of hirudin (0.6 mg/kg bolus followed by 0.2 mg/kg/h) and weight-adjusted heparin. An unacceptably high rate of ICH in both treatment arms forced to terminate prematurely both trials. Using lower doses of both hirudin (0.1 mg/kg bolus followed by 0.1 mg/kg/h) and heparin (not weight adjusted), both trials were continued as TIMI-9b [57] and GUSTO IIb [59]. The TIMI-9b trial showed similar efficacy of heparin and hirudin as adjunctive therapies for SK or t-PA in individuals with acute Q-wave MI without differences in bleeding [57]. The GUSTO IIb trial showed a marginally significant benefit of hirudin over heparin early after infarction in individuals with both Q wave and non–Q-wave MI, which lessened over time [59]. Additionally, results from the Organisation to Assess Strategies for Ischemic Syndromes (OASIS-2) trial showed that recombinant hirudin may be useful when compared with heparin in preventing cardiovascular death, MI, and refractory angina with an acceptable safety profile in patients who have UA/NSTEMI and who receive aspirin [112]. This study (n = 10,141) randomized patients to receive UFH or hirudin (0.4 mg/kg bolus, 0.15 mg/kg/h infusion) for 72 h. This study demonstrated a nonsignificant difference between hirudin and UFH in the primary outcome of cardiovascular death or MI at 7 days: 3.6 % vs.4.2 % had experienced cardiovascular death or new MI (P = 0.077). However, hirudin was associated with a significantly increased risk of major, but not life-threatening, bleeding [112].
A pooled analysis of the OASIS, GUSTO (Global Utilization of Streptokinase and Tissue Plasminogen Activator for Occluded Arteries)-2B, and TIMI-9B trials showed superiority of hirudin compared with UFH for the prevention of death or MI at 30–35 days [112]. However, the only approved clinical application for this agent is in the treatment of HIT recombinant hirudin (lepirudin). Because of a reduced risk for new thromboembolic complications, lepirudin-treated patients had consistently lower incidences of combined endpoints primarily when compared with historical controls [12].
Dosage
The dosage of IV hirudin is 0.15 mg/kg/h infusion with or without 0.4 mg/kg initial bolus [113]. Monitoring is required and it has a narrow therapeutic window. During treatment with lepirudin, aPTT ratios of 1.5:2.5 produce optimal clinical efficacy with a moderate risk for bleeding, aPTT ratios lower than 1.5 are subtherapeutic, and aPTT levels greater than 2.5 are associated with high bleeding risk. The plasma half‐life of hirudins is 60 min following IV injection [114]. Renal clearance is the predominant way of elimination of this drug. Adjustment of dose of lepirudin is recommended for patients with severe renal impairment (<30 ml/min) reducing the dose by a factor of six compared with that given to patients with normal renal function. Monitoring of aPTT should be necessary to further adjust if it exceeds two times baseline. In moderate (CrCl 31–60 ml/min) and mild renal impairment (CrCl 61–90 ml/min) adjustment is not recommended initially but if peak aPTT exceeds two times baseline it is necessary to reduce the dose by half [114].
Side Effects and Contraindications
In settings where anticoagulation is contraindicated hirudin should not be used. In the setting of concomitant anticoagulation or platelet inhibitors, the risk of bleeding with hirudin is increased. In patients with renal dysfunction hirudin should not be used (given that it is renally cleared). Antibody formation to hirudin can be induced for this agent in up to 40 % of patients, presenting anaphylaxis after a re-exposure [113].
Argatroban
Mechanism of Action and Pharmacokinetic/Pharmacodynamic Profile
Argatroban or (2R,4R) 4-methyl-[N2-(3-methyl-1,2,3,4-tetrahydro-8-quinolinyl) sulfonyl]-2-piperidine carboxylic acid is a small-molecule with potent direct competitive thrombin inhibiting effects [12]. This drug is a synthetic N2-substituted arginine derivative that binds to the catalytic site of thrombin with high affinity. Itbinds noncovalently and rapidly to both clot-bound and soluble thrombin, forming in that way a reversible complex [115, 116]. Argatroban is metabolized via the cytochrome P450 3A4 pathway in the liver with a half‐life of 45 min. Rapid restoration of normal hemostasis on cessation of therapy is allowed by the reversible binding of the agent. Argatroban has a predictable dose response that correlates with changes in anticoagulant parameters.
Indications
The use of argatroban has been evaluated primarily as adjunctive therapy with fibrinolytics, in the treatment of HIT, or in patients undergoing PCI [40, 49]. At present there are still limited data with argatroban and it is approved only for use in HIT [117].
Dosages
Argatroban is administered in individuals with unstable angina at a dose of 0.5–5.0 μg/kg/min for 4 h. Dose adjustment with renal impairment is unnecessary, but in patients with renal failure it should be used with caution [118].
Side Effects and Contraindications
Argatroban should not be used in patients who have contraindications to anticoagulant therapy. Argatroban metabolism occurs in the liver. The maximum concentration and half-life of argatroban are increased approximately two- to threefold and clearance is one fourth in patients with hepatic impairment compared with healthy volunteers.
Bivalirudin
Mechanism of Action and Pharmacokinetic/Pharmacodynamic Profile
Bivalirudin is a 20-amino acid polypeptide and is a synthetic version of hirudin [119]. Its amino-terminal D-Phe-Pro-Arg-Pro domain, which interacts with active site of thrombin, is linked via 4 Gly residues to adodecapeptide analogue of the carboxy-terminal of hirudin (thrombinexosite) [120] (Fig. 3.3). Bivalirudin forms a 1:1 stoichiometric complex with thrombin, but once bound, the amino terminal of bivalirudin is cleaved by thrombin, thereby restoring thrombin activity [121].
The half-life of bivalirudin is 25 min [122]. Its clearance is mediated by proteolysis, hepatic metabolism, and renal excretion [123]. Severe renal impairment prolongs the half-life of bivalirudin, and dose adjustment is required for dialysis [124]. Bivalirudin is not immunogenic, in contrast to hirudin, although antibodies against hirudin can cross-react with bivalirudin, the clinical consequences of which are unknown [113].
Indications and Dosage
The use of bivalirudin is supported by wide clinical trial experience. These include supstream treatment in patients with UA/NSTEMI [125, 126],across the spectrum of patients of patients undergoing PCI, including patients with STEMI undergoing primary PCI as an alternative to UFH plus GPI [127], in patients undergoing CABG [128], and in HIT [129]. The recommended dose in PCI is a bolus of 0.75 mg/kg followed by an infusion of 1.75 mg/kg/h for up to 4 h after the procedure as clinically warranted. After cessation of the 1.75 mg/kg/h infusion, a reduced infusion dose of 0.25 mg/kg/h may be continued for 4–12 h as clinically necessary.
Evidence for Use: UA/NSTEMI
Bivalirudin and UFH were compared in the Randomized Evaluation in Percutaneous Coronary Intervention Linking Angiomax to Reduced Clinical Events 2(REPLACE-2) study [130]. This trial (n = 6,010) enrolled patients undergoing urgent or elective PCI who were randomized to receive bivalirudin with provisional GPI or UFH with planned GPI. The study demonstrated the noninferiority of bivalirudin compared to UFH plus GPI regarding ischemic end point sand showed also a significant association with less major and minor bleeding [130].
In the Acute Catheterization and Urgent Intervention Triage Strategy (ACUITY) study, patients with UA/NSTEMI (n = 13,189) were randomized to one of three antithrombotic regimens: UFH or enoxaparin plus GPI, bivalirudin plus GPI, or bivalirudin alone [125]. Compared with UFH plus a GPI, bivalirudin plus a GPI, was associated with noninferior 30-day rates of the composite ischemia end point (7.7 and 7.3 %, respectively), major bleeding (5.3 and 5.7 %), and the net clinical outcome end point (11.8 and 11.7 %). Compared with UFH plus a GPI, bivalirudin alone was associated with a noninferior rate of the composite ischemia end point (7.8 and 7.3 %, respectively; P = 0.32) and significantly reduced rates of major bleeding (3.0 % vs. 5.7 %; P < 0.001) and the net clinical outcome end point (10.1 % vs. 11.7 %; P = 0.02). Essentially, compared with UFH bivalirudin in addition to GPI presented similar rates of ischemia and bleeding, but bivalirudin alone was also associated with significantly lower rates of bleeding [125].
However, the ACUITY trial presented some important limitations that need to be addressed: first, not all patients were pretreated with clopidogrel (and used inconsistent dosing) and two thirds of them were already receiving some anticoagulant before randomization and, with the resultant variability among the treatments before and during the study. Moreover the election of type of GPI and UFH/LMWH was left at the discretion of physician. Lastly, the ACUITY trial has been also criticized because of its quite liberal definition of bleeding, particularly concerning the definition of major bleeding [125].
The Intracoronary Stenting and Antithrombotic Regimen: Rapid Early Action for Coronary Treatment 4 (ISAR REACT 4) trial [126] was a double-blind randomized trial which design tried to overcome the aforementioned limitations of the ACUITY trial. The same GPI (abciximab) was used and all patients received a 600-mg-clopidogrel pretreatment. In addition, less liberal definitions of major bleeding were considered (presence of intracranial, intraocular, or retroperitoneal hemorrhage; a decrease in the hemoglobin level of > 40 g/L plus either overt bleeding or the need for transfusion of 2 or more units of packed red cells or whole blood). Specifically, the ISAR REACT 4 trial compared abciximab and heparin versus bivalirudin in NSTEMI patients undergoing PCI (n = 1,721) showing that such regimen compared to bivalirudin increased the risk of bleeding in NSTEMI patients undergoing PCI (P = 0.02) and failed to decrease the rate of ischemic events (P = 0.76) [126]. Current guidelines for UA/NSTEMI recommend to omit administration of an IV GPI if bivalirudin is selected as the anticoagulant and at least 300 mg of cvs. lopidogrel was administered at least 6 h earlier than planned catheterization or PCI (Class IIa, LOE B) [40, 65] (Table 3.1).
Evidence for Use: STEMI
In the Harmonizing Outcomes with Revascularization and Stents in Acute Myocardial Infarctions (HORIZONS-AMI) trial, [127] STEMI patients (n = 3,602) who presented within 12 h after onset of symptoms were randomized to UFH plus GPI or to treatment with bivalirudin alone for primary PCI. At 30 days, compared with UFH plus GPI, bivalirudin alone demonstrated lower rates of death (P = 0.047) and major bleeding (P < 0.001), leading to a significantly lower rate of net adverse clinical events (P = 0.005). The rates of cardiac mortality (P = 0.005) and all-cause mortality (P = 0.037) were significantly lower in the bivalirudin alone treatment group after 1 year (p = 0.001) [131] and 3 years (P = 0.03) [132]. Regarding stent thrombosis (ST), the study found that within the first 24 h ST occurred more commonly in patients on bivalirudin compared with those assigned to heparin plus a GPI [127]. Nevertheless, between 24 h and 1 year, ST was more frequent in the heparin plus GPI group than in the bivalirudin group (46 vs. 36 ST events, respectively). Thus, at the end of the 1-year follow-up, the rate of ST was similar in the two groups (3.1 % vs. 3.5 %, respectively, P =0.53) [131]. However, the hazard ratio for death within the first month was greater after major bleeding than after reinfarction or ST [127]. In a post-hoc analysis, mortality and major bleeding were shown to be significantly higher after in-hospital ST compared with out-of-hospital ST (p < 0.01 for both events). Randomization to UFH plus GPI (vs. bivalirudin) was additionally correlated with increased mortality after ST [133].
Another post-hoc analysis of this study [134] showed that 600-mg-clopidogrel loading dose compared with 300 mg had significantly lower 30-day unadjusted rates of definite or probable ST (1.7 % vs. 2.8 %, p = 0.04), as well as lower mortality (p = 0.03) and reinfarction (p = 0.02), and, without higher bleeding rates. Bivalirudin monotherapy resulted in similar reductions in net adverse cardiac event rates with both doses (p-interaction = 0.41). However, a 600-mg-clopidogrel loading dose was an independent predictor of lower rates of 30-day major adverse cardiac events (p = 0.04) [134].
In the setting of STEMI, current guidelines recommended bivalirudin over UFH and a GPI, restricting the use of GPI to bailout (Class I, LOE B) [49, 50]. In patients at high risk of bleeding the American guidelines recommended bivalirudin preferred over UFH with GPI (Class IIa, LOE B) [49] (Table 3.2).
Factor Xa Inhibitors
Fondaparinux
Mechanism of Action and Pharmacokinetic/Pharmacodynamic Profile
Fondaparinux is a selective indirect FXa inhibitor. This compound, with a molecular weight of 1,728 Da, is a synthetic analog of the unique AT-binding pentasaccharide sequence found in UFH. It binds reversibly to AT and provokes an irreversible conformational change at the reactive site of AT that increases its reactivity with FXa [135]. Fondaparinux is available to activate additional AT molecules after being released from AT, but it does not increase the rate of thrombin inhibition by AT because it is too short to bridge AT to thrombin. Fondaparinux does not have any inhibitory action against thrombin that is already formed, even though it inhibits FXa-dependent thrombin generation [113]. After SC injection the bioavailability is 100 % [136, 137]. The elimination half-life is 17 h with primary renal clearance. Fondaparinux is contraindicated in patients with severe renal impairment [40, 113, 138]. The anticoagulant response of fondaparinux is predictable and its PK profile is linear when it is given in SC doses of 2–8 mg or in IV doses ranging from 2 to 20 mg that result in anti-Xa activity that is roughly 7 times that of LMWHs [136, 137]. Its minimal nonspecific binding to plasma proteins it is likely to be the reason of its excellent bioavailability and predictable anticoagulant response [139].
Even if monitoring is not required, the anticoagulant effect of fondaparinux can be measured in anti-FXa units. Fondaparinux does not affect other parameters of anticoagulation, including aPTT, activated clotting time, or prothromb in time [138] and does not induce the formation of UFH:platelet factor-4 complexes and does not cross-react with HIT antibodies. Therefore, it is unlikely that induces HIT [140]. Not with Standing fondaparinux is not labeled for treatment of HIT, it has been used successfully to treat HIT patients [141, 142].It is unlikely that fondaparinux induces osteoporosis because it has no effect on osteoblasts [143]. In pregnancy, fondaparinux has not been studied enough, but it does not seem to cross the placental barrier [144].
Dosing, Monitoring, Reversal
Fondaparinux 2.5 mg daily was shown to have the best efficacy/safety profile when compared with 4-, 8-, and 12-mg doses of fondaparinux and with enoxaparin 1 mg/kg b.i.d based on a dose-ranging study of fondaparinux versus enoxaparin in the setting of UA/NSTEMI involving 1,147 patients [145].
There are no data about coagulation monitoring as part of the clinical development of this drug, but currently it is not recommended. Also in patients with severe renal impairment (CrCl <30 mL/min), fondaparinux has not been adequately studied. However, it is established that in patients with moderate renal impairment (30–50 mL/min) fondaparinux dose should be reduced in half or low-dose heparin should be used in place of fondaparinux [113].To assess the anticoagulant effect of fondaparinux it is possible to measure anti-Xa levels, but the standard therapeutic level is unknown. It is important to highlight that protamine is not able to reverse the anticoagulant effect of fondaparinux. Recombinant factor VIIa may be given to achieve the reversal of the anticoagulant effect of fondaparinux if life-threatening bleeding takes place due to this agent [146].
Indications
Evidence for Use: UA/NSTEMI
The large-scale OASIS-5 trial (n = 20,078) evaluated the efficacy and safety of fondaparinux (2.5 mg SC daily) compared with enoxaparin (1 mg/kg SC b.i.d) in patients with UA/NSTEMI [147]. Fondaparinux demonstrated to be noninferior vs. enoxaparin in the primary outcome of combined death, MI, or refractory ischemia at 9 days was achieved (P = 0.007), with less incidence of major bleeding (P < 0.001). Fondaparinux showed a superior net clinical benefit (composite of death, MI refractory ischemia, or major bleeding) compared with enoxaparin (P < 0.001). Compared with enoxaparin, fondaparinux also demonstrated significantly a reduction in mortality at 6 months (P = 0.05). Nevertheless, more catheter-related thrombus formation occurred with fondaparinux in the group of patients who underwent PCI (P < 0.001), showing that anticoagulation with fondaparinux alone is not enough for PCI, therefore another anticoagulant with FIIa activity (such as UFH) must be coadministered [147].
The main disadvantage of fondaparinux in this trial was the excess of catheter thrombosis seen in patients undergoing PCI (0.9 % vs. 0.4 % with enoxaparin), which has limited the widespread use of the drug in this setting. For that reason the OASIS investigators conducted The Fondaparinux Trial With UFH During Revascularization in ACS (FUTURA/OASIS-8) trial (n = 2,026) in order to evaluate the safety of 2 dose regimens of adjunctive IV UFH during PCI in high-risk patients with NSTEMI initially treated with fondaparinux [148]. Patients received either low-dose UFH (50 U/kg, regardless of use of GPI) or standard-dose UFH (85 U/kg or 60 U/kg with GPI), adjusted by activated clotting time. In terms of preventing peri-PCI major bleeding or major vascular access-site complications, low fixed-dose of UFH was not superior to standard ACT-guided UFH. Thrombotic events were not significantly different between the treatment groups (P = 0.27). Catheter thrombosis rates were very low (0.5 % in the low-dose group and 0.1 % in the standard-dose group, P = 0.15) [148].
Based on the above, current guidelines recommended 2.5 mg SC once daily as having the most favorable efficacy-safety profile with respect to anticoagulation (Class I, LOE A), avoiding its use for CrCl <30 mL/min. During PCI, at present2012 American guidelines recommend an additional 50–60 IU/kg IV bolus of UFH [40] while European guidelines recommend a 85 IU/kg IV bolus of UFH (adapted to ACT) or 60 IU in the case of concomitant use of GPI (Class I, LOE B) [41] (Table 3.1).
Evidence for Use: STEMI
In the OASIS-6 trial (n = 12,092), fondaparinux was evaluated as an alternative to standard adjunctive anticoagulation in patients with STEMI [149]. Patients received 2.5 mg SC daily for 8 day and it was compared with either no UFH (stratum I) or UFH infusion (stratum II) for 48 h. In approximately 25 % of patients primary PCI was performed. Approximately half the patients received fibrinolytic therapy, of whom 73 % received SK. In patients who received fondaparinux, the primary outcome of 30-day death or MI was significantly reduced (P = 0.008), although this was driven by patients in stratum I only. No significant benefit with fondaparinux was found in patients who underwent primary PCI or who were in stratum II. It is important to highlight that, compared with UFH, patients who underwent primary PCI with fondaparinux presented more catheter-related thrombi (P < 0.001), more coronary complications (P = 0.04), and a trend toward higher death or MI compared with UFH (P = 0.19).
Importantly, although European guidelines recommend using of fondaparinux in ACS [50], in the United States fondaparinux is not currently approved for such use by the FDA, as it is reflected in the current ACC guidelines for STEMI [49], because of the risk of catheter thrombosis (Class III, LOE: B) (Table 3.2).
As adjunctive antithrombotic therapy to support reperfusion with fibrinolytic therapy an initial IV dose of 2.5 mg of fondaparinux is recommended, and then 2.5 mg subcutaneously daily starting the following day for the index hospitalization up to 8 days or until revascularization. Fondaparinux is contraindicated if CrCl <30 mL/min [49] (Table 3.2).
Otamixaban
Otamixaban is a specific direct parenteral small molecule that inhibits clot-bound FXa which is inaccessible to large molecule or indirect inhibitors agent. Otamixaban is not still under advanced clinical investigation and not approved for clinical use. However, this agent has shown thus far promising results. Otamixaban has a very favorable PK/PD profile: short acting (half-life30 min), weight based bolus (vs. infusion), no need for monitoring and no significant renal elimination (<25 %), but no antidote to otamixaban has been described [150].
In the Randomized, double-blind, dose-ranging study of otamixaban, a novel, parenteral, short-acting direct factor Xa inhibitor, in percutaneous coronary intervention (the SEPIA-PCI trial) [151], 947 patients were randomly assigned to either 1 of 5 weight-adjusted otamixaban regimens or weight-adjusted UFH before PCI. The study showed that otamixaban reduced the median change in prothrombin fragments 1 + 2 (F1 + 2) significantly more than UFH at the highest dose regimen (P = 0.008), without significant difference in the incidence of TIMI bleeding compared with UFH [151]. Posteriorly, another phase II study: the Otamixaban for the treatment of patients with non-ST-elevation acute coronary syndromes (SEPIA-ACS1 TIMI 42) trial (n = 3,241) assessed the dose response on death, MI, urgent revascularization or bail-out in patients with NSTE-ACS with encouraging results about safety and efficacy, concluding that otamixaban 0.105–014 mg/kg/h appeared to be best range for further study as a replacement of UFH and GPI [152]. The ongoing phase III Treatment of Acute coronary syndromes with Otamixaban (TAO) trial (n = 13,240) is evaluating the efficacy of otamixaban vs. UFH with and without eptifibatide on reduction of death or MI in patients with NSTE-ACS [153].
Oral Anticoagulant Therapy in ACS
The main reasons of the necessity of long term anticoagulation in ACS patients is the coexistence of another indication for anticoagulant therapy, such as atrial fibrillation (AF), left ventricular thrombus, advanced heart failure, deep venous thrombosis, prosthetic heart valves, or history of pulmonary embolism. The main risk in these patients remains the concomitant use of antiplatelet therapy and the consequent high risk of bleeding. Although, in the ACS population a considerable reduction in cardiovascular events is obtained with long-term dual antiplatelet therapy (DAPT) using aspirin and a P2Y12 platelet receptor, [154] the risk of a recurrent vascular event within 12 months is still high and underscores the necessity of better secondary prevention strategies. Given that levels of thrombin generation persist elevated in ACS patients [155], and given the role of thrombin on arterial thrombogenesis, long-term use of oral anticoagulant strategies have been considered for secondary prevention of coronary events. Initially, vitamin K antagonists (VKAs) have been studied, mostly in combination with aspirin. However, despite the proven efficacy of this strategy, bleeding rates were high. The development of novel oral anticoagulants (NOACs) characterized by a better safety profile compared with VKAs have led to reconsider long-term oral anticoagulant therapy in adjunct to standard of care antiplatelet therapy, including mostly DAPT with aspirin and clopidogrel, also known as “triple therapy”. The role of oral anticoagulant therapy for secondary prevention of ischemic events is described below.
Vitamin K Antagonists
VKAs, warfarin and coumarin derivatives, have demonstrated to reduce the risk of recurrent ischemic events both in monotherapy and in combination with aspirin [156]. In the Warfarin-Aspirin Re-Infarction Study (WARIS II) study, in combination with aspirin or given alone, warfarin was superior to aspirin alone in reducing the incidence of composite events after an acute MI but was associated with a higher risk of bleeding [156]. In this study, the combination therapy targeted an international normalized ratio (INR) of 2–2.5 and the warfarin alone group had a target INR of 2.8–4.2. No reduction in the combined risk of cardiovascular death, reinfarction, or stroke was demonstrated using a fixed, low dose of warfarin added to aspirin in the long term after MI, but this combination reduced the risk of stroke (secondary endpoint). An increased risk of bleeding was also associated to the concomitant administration of aspirin and warfarin [157]. These bleeding rates are even higher in patients who are on DAPT with aspirin and clopidogrel, as have been studied mostly in patients with AF undergoing PCI [158–160], the details of which going beyond the scope of this chapter which is focused on the role of anticoagulants for secondary prevention of ischemic recurrences in ACS.
Currently available data based on around 20,000 patients from randomized clinical trials show that oral anticoagulant therapy (given in adequate doses) reduce the rates of re-infarction and thromboembolic stroke, but increasing significantly the rates of hemorrhagic events [161]. Nevertheless, even in controlled trials the use of warfarin, presents several difficulties. For example, in the WARIS II study the INR was below target in about one third of patients, and those over 75 years of age were excluded [161].
Novel Oral Anticoagulants for Secondary Prevention
Several classes of NOACs have been developed. These agents have been primarily studied in patients with AF [162–164] and characterized by more favorable safety profile, particularly a lower risk of ICH, with comparable or better efficacy compared with VKAs. These agents also have the advantage of less drug-drug and food-drug interactions and that they can be administered in fixed doses without routine coagulation monitoring.
There are some aspects that should be considered for the periprocedural management of NOACs, compared with warfarin, such as their shorter half-life or the fact that the onset of their effects is within 2 h, provided that intestinal absorption is normal. Table 3.3 summarizes the recommendations for NOACs in patients undergoing elective PCI. The current status of knowledge of NOACs for secondary prevention of ischemic events in ACS is described below.
Oral Direct Thrombin Inhibitors
Ximelagatran
The Efficacy and Safety of the Oral Direct Thrombin Inhibitor Ximelagatran in Patients with Recent Myocardial Damage (ESTEEM) trial for the secondary prevention of ACS showed a 24 % relative reduction with ximelagatran plus aspirin treatment for 6 months in the risk of the primary composite end point of death, nonfatal MI, and severe recurrent ischemia versus aspirin alone, although this occurred at the expense of an increased risk of bleeding [168]. Ximelagatran was retrieved from the market due to safety concerns (hepatic toxicity), but did provide encouraging results for other NOACs to be tested for secondary prevention in ACS.
Dabigatran Etexilate
Dabigatran etexilate binds reversibly and directly to the catalytic site of thrombin. It is a synthetic low molecular weight eptidomimetic generated as a prodrug which immediately after absorption is biotranformed by an esterase-mediated hydrolysis to the active compound dabigatran [169–171] Table 3.4 summarizes the principal pharmacologic characteristics of dabigatran etexilate compared with the new FXa inhibitors [173].
The Dose Finding Study for Dabigatran Etexilate in Patients With Acute Coronary Syndrome (RE-DEEM) trial (n = 1,861) was a phase II study that showed a dose-dependent increase in clinically relevant bleeding events, with highest rates with dose regimens currently used in AF (110 mg and 150 mg b.i.d.) [174]. Despite higher dabigatran doses compared with lower doses and placebo group seemed to have some benefit, it was impossible to demonstrate an efficacy difference in cardiovascular death, nonfatal MI or nonhemorrhagic stroke because the lack of enough statistical power of the trial [174]. Phase III clinical testing of dabigatran in ACS has not been pursued.
Oral Direct Factor Xa Inhibitors
Rivaroxaban, apixaban and darexaban are the three oral direct FXa inhibitors that have been most studied over recent years. Table 3.4 summarize the principal pharmacologic characteristics and dosages of these drugs compared with dabigatran [173, 175].
Darexaban (YM150)
The safety, tolerability, and regimen of darexaban for the prevention of ischemic events in ACS were evaluated in The Study Evaluating Safety, Tolerability and Efficacy of YM150 in Subjects With Acute Coronary Syndromes (RUBY-1) trial [176]. The study did not find benefits regarding an addition of efficacy to DAPT in this setting, but showed an expected dose-related 2- to 4-fold increase in bleeding versus placebo as the only safety concern [176]. Darexaban development has been discontinued.
Apixaban
The phase II Apixaban for Prevention of Acute Ischemic and Safety Events (APPRAISE) trial found a dose-dependent interaction increased risk of bleeding complications with apixaban 2.5 mg b.i.d. and 10 mg q.d. Although apixaban was associated with a numerically lower incidence of cardiovascular death, MI, severe recurrent ischemia, or ischemic stroke it was not statistically significant [177]. APPRAISE-2 trial (n = 7,392) failed also to find similar benefits of adding a high dose of apixaban (5 mg b.i.d) to single or DAPT in a very-high-risk ACS population [178]. A greater number of intracranial and fatal bleeding events happened with apixaban than with placebo, without a significant reduction in recurrent ischemic events. Because of the wide CIs allow for either benefit or harm, the overall efficacy/safety balance of apixaban is still unknown.
Rivaroxaban
The Rivaroxaban in Combination With Aspirin Alone or With Aspirin and a thienopyridine in Patients With Acute Coronary Syndromes-TIMI 46 (ATLAS ACS-TIMI 46) trial [179], demonstrated either in patients receiving aspirin alone and in patients receiving DAPT, a rivaroxaban dose-dependent increased risk of clinically significant bleeding complications. However, the lower doses were associated with lowest bleeding risk and accompanied by an ischemic benefit. This set the basis for developing the phase III ATLAS ACS-2–TIMI 51 (ATLAS-2) trial (n = 15,526) demonstrated that both rivaroxaban regimens (2.5-mg and 5-mg b.i.d.) compared with placebo significantly reduced the primary efficacy composite of cardiovascular death, MI, or stroke (p < 0.008) [180, 181]. The 2.5-mg (but not 5-mg b.i.d.), reduced cardiovascular and all-cause mortality. The 5 mg b.i.d. (but not 2.5-mg b.i.d.) reduced MI. Both dosages of rivaroxaban reduced significantly the risk of ST, as compared with placebo (P = 0.02), while the 2.5-mg b.i.d dose showed a nonsignificant but directionally consistent benefit for MI. The rates of non-CABG-related TIMI major bleeding and ICH with both doses were significantly increased, without a significant increase in fatal bleeding (P = 0.66) [180, 181].
Current ESC Guidelines for the management of STEMI recommended considering the use of low-dose rivaroxaban (2.5 mg b.i.d) in selected patients who receive aspirin and clopidogrel, if the patient is at low bleeding risk (Class of recommendation IIb, LOE B) [50]. Rivaroxaban however is still not approved for clinical use in the United States.
Anticoagulants Under Clinical Development
Aptamers are small oligonucleotides that form unique sequence-dependent three-dimensional structures [182, 183] and can be developed to inhibit specific protein targets with high affinity and used as active drugs. Aptamers provide the code for their own complement (reversal agent), which can be developed and used to inhibit their function [183–185]. Reversal of aptamer activity can be titrated to the patient’s clinical condition given that the degree of reversal is directly related to the molar ratio of administered components. The REG1 anticoagulation system is a novel, aptamer-based, FIXa inhibitor that is being described for use in patients undergoing PCI. This system consists of pegnivacogin, a single-stranded RNA factor IXa inhibitor, and its complementary reversal agent, anivamersen, which binds to and inactivates pegnivacogin with rapid kinetics [186]. Phase I [185–188] and phase II [189] studies investigated REG1 with encouraging results. Recently, the RADAR (A Randomized, Partially Blinded, Multicenter, Active-Controlled, Dose-Ranging Study Assessing the Safety, Efficacy, and Pharmacodynamics of the REG1 Anticoagulation System in Patients with ACS) trial [190, 191] showed that at least 50 % anivamersen-mediated reversal of pegnivacogin was necessary to effectively diminish bleeding after early femoral sheath removal in invasively managed patients with ACS. To determine the safety and efficacy of REG1 more powered randomized clinical trials are needed. A large scale phase III clinical trial is currently planned.
Thrombin generation is also decreased by drugs that target coagulation proteases that drive the propagation phase. Coagulation proteases modulate inflammation by activating protease activated receptors (PARs), and by binding to other cell surface receptors, such as Thrombomodulin (TM) and endothelial protein C receptor (EPCR) [192, 193]. PAR-2 does not bind thrombin, but the tissue factor (TF)/FVIIa complex and FXa can activate this receptor [194]. Activation of PARs by the various coagulation proteases results in the upregulation of genes involved in inflammation, including interleukin (IL)-8 and tumour necrosis factor (TNF)-α. TF/FVIIa-induced signaling events can modulate cell fate and behaviour, rendering cells and tissues proliferative, pro-migratory, and resistant to apoptosis. Based on these findings, PAR inhibitors are under development and PAR-1-targeting drugs have undergone phase III clinical trial evaluation [195, 196]. In addition to the role of PARs in inflammation, additional cross-talk occurs at the level of FXa. This concept is highlighted by the recent demonstration that lufaxin, a FXa inhibitor from the salivary glands of blood-sucking arthropods, not only inhibits thrombosis in mice, but also attenuates oedema formation triggered by FXa injection into their paws [197]. Other anticoagulant therapies in development that block target coagulationproteases that drive the propagation phase, such as FVIIIa (TB-402), or jointly FVa/FVIIIa, cofactors that are critical for the generation of thrombin (drotrecogin, which is a recombinant form of human activated protein C and recomodulin and solulin, both of which are recombinant soluble derivatives of human thrombomodulin). Inhibitors toward the TF/FVIIa complex, such as recombinant TFPI (tifacogin), recombinant nematode anticoagulant protein (NAP)C2, active site–inhibited recombinant (r) FVIIa inhibitors (rFVIIaI) and monoclonal antibodies against TFT have been developed to target the initiation of coagulation [107] (Fig. 3.4).
Conclusions
During the last decade antithrombotic treatment of ACS has changed very rapidly, particularly due to the development of new antiplatelet and anticoagulant agents. The coagulation cascade offers numerous potential targets of treatment that allow interfering in many processes of haemostasis and thrombosis. Currently, there are a plethora of new parenteral and oral anticoagulants that are being developed and incorporated into clinical practice in the setting of ACS. Many of these are preferred over older treatment regimens because of their more favorable safety profile. However, others still require to be refined and ongoing clinical trials will provide more insights on the safety and efficacy of these strategies.
References
Davies MJ, Thomas AC. Plaque fissuring-the cause of acute myocardial infarction, sudden ischaemic death, and crescendo angina. Br Heart J. 1985;53:363–73.
Farb A, Burke AP, Tang AL, et al. Coronary plaque erosion without rupture into a lipid core. A frequent cause of coronary thrombosis in sudden coronary death. Circulation. 1996;93(7):1354–63.
Braunwald E. Unstable angina and non-ST elevation myocardial infarction. Am J Respir Crit Care Med. 2012;185:924–32.
Furie B, Furie BC. Molecular and cellular biology of blood coagulation. N Engl J Med. 1992;326:800–6.
Chesebro JH, Zoldhelyi P, Badimon L, et al. Role of thrombin in arterial thrombosis: implications for therapy. Thromb Haemost. 1991;66:1–5.
Bar-Shavit R, Eldor A, Vlodavsky I. Binding of thrombin to subendothelial extracellular matrix: protection and expression of functional properties. J Clin Invest. 1989;84:1096–104.
Hogg PJ, Jackson CM. Fibrin monomer protects thrombin from inactivation by heparin-antithrombin III: implications for heparin efficacy. Proc Natl Acad Sci USA. 1989;86:3619–23.
Weitz JI, Hudoba M, Massel D, et al. Clot-bound thrombin is protected from inhibition by heparinantithrombin III but is susceptible to inactivation by antithrombin III-independent inhibitors. J Clin Invest. 1990;86:385–91.
Weitz JI, Leslie B, Hudoba M. Thrombin binds to soluble fibrin degradation products where it is protected from inhibition by heparin-antithrombin but susceptible to inactivation by antithrombin- independent inhibitors. Circulation. 1998;97:544–52.
Serruys P, Vranckx P, Allikmets K, et al. Clinical development of bivalirudin (angiox): rationale for thrombin-specific anticoagulation in percutaneous coronary intervention and acute coronary syndromes. Int J Clin Pract. 2006;60:344–50.
De Caterina R, Husted S, Wallentin L, et al. General mechanisms of coagulation and targets of anticoagulants (Section I). Position Paper of the ESC Working Group on Thrombosis – Task Force on Anticoagulants in Heart Disease. Thromb Haemost. 2013;109:569–79.
Di Nisio M, Middeldorp S, Buller HR. Direct thrombin inhibitors. N Engl J Med. 2005;353:1028–40.
McAllister BM, Demis DJ. Heparin metabolism: isolation and characterization of uroheparin. Nature. 1966;212:293–4.
Hirsh J. Heparin. N Engl J Med. 1992;324:1565–74.
Hull RD, Raskob GE, Hirsh J, et al. Continuous intravenous heparin compared with intermittent SC heparin in the initial treatment of proximal-vein thrombosis. N Engl J Med. 1986;315:1109–14.
Hirsh J, van Aken WG, Gallus AS, et al. Heparin kinetics in venous thrombosis and pulmonary embolism. Circulation. 1976;53:691–5.
Young E, Prins M, Levine MN, et al. Heparin binding to plasma proteins, an important mechanism for heparin resistance. Thromb Haemost. 1992;67:639–43.
Barzu T, Molho P, Tobelem G, et al. Binding and endocytosis of heparin by human endothelial cells in culture. Biochim Biophys Acta. 1985;845:196–203.
Sobel M, McNeill PM, Carlson PL, et al. Heparin inhibition of von Willebrand factordependent platelet function in vitro and in vivo. J Clin Invest. 1991;87:1787–93.
Friedman Y, Arsenis C. Studies on the heparin sulphamidase activity from rat spleen. Intracellular distribution and characterization of the enzyme. Biochem J. 1974;139:699–708.
Dawes J, Papper DS. Catabolism of low-dose heparin in man. Thromb Res. 1979;14:845–60.
de Swart CA, Nijmeyer B, Roelofs JM, et al. Kinetics of intravenously administered heparin in normal humans. Blood. 1982;60:1251–8.
Olsson P, Lagergren H, Ek S. The elimination from plasma of intravenous heparin. An experimental study on dogs and humans. Acta Med Scand. 1963;173:619–30.
Bjornsson TD, Wolfram KM, Kitchell BB. Heparin kinetics determined by three assay methods. Clin Pharmacol Ther. 1982;31:104–13.
Theroux P, Ouimet H, McCans J, et al. Aspirin, heparin, or both to treat acute unstable angina. N Engl J Med. 1988;319:1105–11.
Granger CB, Miller JM, Bovill EG, et al. Rebound increase in thrombin generation and activity after cessation of intravenous heparin in patients with acute coronary syndromes. Circulation. 1995;91:1929–35.
Theroux P, Waters D, Lam J, et al. Reactivation of unstable angina after the discontinuation of heparin. N Engl J Med. 1992;327:141–5.
Heiden D, Mielke Jr CH, Rodvien R. Impairment by heparin of primary haemostasis and platelet [14C]5-hydroxytryptamine release. Br J Haematol. 1977;36:427–36.
Eika C. Inhibition of thrombin-induced aggregation of human platelets by heparin and antithrombin 3. Scand J Haematol. 1971;8:250–6.
Kelton JG, Hirsh J. Bleeding associated with antithrombotic therapy. Semin Hematol. 1980;17:259–91.
Blajchman MA, Young E, Ofosu FA. Effects of unfractionated heparin, dermatan sulfate and low molecular weight heparin on vessel wall permeability in rabbits. Ann N Y Acad Sci. 1989;556:245–54.
Clowes AW, Karnowsky MJ. Suppression by heparin of smooth muscle cell proliferation in injured arteries. Nature. 1977;265:625–6.
Castellot Jr JJ, Favreau LV, Karnovsky MJ, et al. Inhibition of vascular smooth muscle cell growth by endothelial cell-derived heparin. Possible role of a platelet endoglycosidase. J Biol Chem. 1982;257:11256–60.
Shaughnessy SG, Young E, Deschamps P, et al. The effects of low molecular weight and standard heparin on calcium loss from fetal rat calvaria. Blood. 1995;86:1368–73.
Bhandari M, Hirsh J, Weitz JI, et al. The effects of standard and low molecular weight heparin on bone nodule formation in vitro. Thromb Haemost. 1998;80:413–7.
Visentin GP, Ford SE, Scott JP, et al. Antibodies from patients with heparin-induced thrombocytopenia/thrombosis are specific for platelet factor 4 complexed with heparin or bound to endothelial cells. J Clin Invest. 1994;93:81–8.
Greinacher A, Liebenhoff U, Kiefel V, et al. Heparin-associated thrombocytopenia: the effects of various intravenous IgG preparations on antibody mediated platelet activation -a possible new indication for high dose i.v. IgG. Thromb Haemost. 1994;71:641–5.
Whitfield LR, Lele AS, Levy G. Effect of pregnancy on the relationship between concentration and anticoagulant action of heparin. Clin Pharmacol Ther. 1983;34:23–8.
Edson JR, Krivit W, White JG. Kaolin partial thromboplastin time: high levels of procoagulants producing short clotting times or masking deficiencies of other procoagulants or low concentrations of anticoagulants. J Lab Clin Med. 1967;70:463–70.
Jneid H, Anderson JL, Wright RS, et al. 2012 ACCF/AHA focused update of the guideline for the management of patients with unstable angina/Non-ST-elevation myocardial infarction (updating the 2007 guideline and replacing the 2011 focused update): a report of the American College of Cardiology Foundation/American Heart Association Task Force on practice guidelines. Circulation. 2012;126:875–910.
Hamm CW, Bassand JP, Agewall S, et al. 2011 ESC Guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation: The Task Force for the management of acute coronary syndromes (ACS) in patients presenting without persistent ST-segment elevation of the European Society of Cardiology (ESC). Eur Heart. 2011;J32:2999–3054.
Telford AM, Wilson C. Trial of heparin versus atenolol in prevention of myocardial infarction in intermediate coronary syndrome. Lancet. 1981;1:1225–8.
Williams DO, Kirby MG, McPherson K, et al. Anticoagulant treatment of unstable angina. Br J Clin Pract. 1986;40:114–6.
Theroux P, Waters D, Qiu S, et al. Aspirin versus heparin to prevent myocardial infarction during the acute phase of unstable angina. Circulation. 1993;88:2045–8.
Neri Serneri GG, Gensini GF, Poggesi L, et al. Effect of heparin, aspirin, or alteplase in reduction of myocardial ischaemia in refractory unstable angina. Lancet. 1990;335:615–8.
Holdright D, Patel D, Cunningham D, et al. Comparison of the effect of heparin and aspirin versus aspirin alone on transient myocardial ischemia and in-hospital prognosis in patients with unstable angina. J Am Coll Cardiol. 1994;24:39–45.
Cohen M, Adams PC, Hawkins L, et al. Usefulness of antithrombotic therapy in resting angina pectoris or non-Q-wave myocardial infarction in preventing death and myocardial infarction (a pilot study from the Antithrombotic Therapy in Acute Coronary Syndromes Study Group). Am J Cardiol. 1990;66:1287–92.
Oler A, Whooley MA, Oler J, et al. Adding heparin to aspirin reduces the incidence of myocardial infarction and death in patients with unstable angina. A meta-analysis. JAMA. 1996;276:811–5.
O’Gara PT, Kushner FG, Ascheim DD, et al. 2013 ACCF/AHA guideline for the management of ST-elevation myocardial infarction: a report of the American College of Cardiology Foundation/American Heart Association Task Force on Practice Guidelines. Circulation. 2013;127:e362–425.
Steg PG, James SK, Atar D, Badano LP, et al. ESC Guidelines for the management of acute myocardial infarction in patients presenting with ST-segment elevation. Task Force on the management of ST-segment elevation acute myocardial infarction of the European Society of Cardiology (ESC). Eur Heart J. 2012;33:2569–619.
Antman EM, Morrow DA, McCabe CH, et al. Enoxaparin versus unfractionated heparin with fibrinolysis for ST-elevation myocardial infarction. N Engl J Med. 2006;354:1477–88.
Hirsh J, Warkentin TE, Raschke R, et al. Heparin and low-molecular-weight heparin: mechanisms of action, pharmacokinetics, dosing considerations, monitoring, efficacy, and safety. Chest. 1998;114:489S–510.
Hochman JS, Wali AU, Gavrila D, et al. A new regimen for heparin use in acute coronary syndromes. Am Heart J. 1999;138:313–8.
Morabia A. Heparin doses and major bleedings. Lancet. 1986;1:1278–9.
Antman EM. Hirudin in acute myocardial infarction. Safety report from the Thrombolysis and Thrombin Inhibition in Myocardial Infarction (TIMI) 9A Trial. Circulation. 1994;90:1624–30.
Warkentin TE, Levine MN, Hirsh J, et al. Heparin-induced thrombocytopenia in patients treated with low-molecular-weight heparin or unfractionated heparin. N Engl J Med. 1995;332:1330–5.
Antman EM. Hirudin in acute myocardial infarction. Thrombolysis and Thrombin Inhibition in Myocardial Infarction (TIMI) 9B trial. Circulation. 1996;94:911–21.
The Global Use of Strategies to Open Occluded Coronary Arteries (GUSTO) IIa Investigators. Randomized trial of intravenous heparin versus recombinant hirudin for acute coronary syndromes. Circulation. 1994;90:1631–7.
The Global Use of Strategies to Open Occluded Coronary Arteries (GUSTO) IIb Investigators. A comparison of recombinant hirudin with heparin for the treatment of acute coronary syndromes. N Engl J Med. 1996;335:775–82.
The EPIC Investigators. Use of a monoclonal antibody directed against the platelet glycoprotein IIb/IIIa receptor in high-risk coronary angioplasty. N Engl J Med. 1994;330:956–61.
Arepally GM, Ortel TL. Clinical practice. Heparin-induced thrombocytopenia. N Engl J Med. 2006;355:809–17.
Baroletti SA, Goldhaber SZ. Heparin-induced thrombocytopenia. Circulation. 2006;114:e355–6.
Caplan SN, Berkman EM. Protamine sulfate and fish allergy. N Engl J Med. 1976;295:172.
Stewart WJ, McSweeney SM, Kellett MA, et al. Increased risk of severe protamine reactions in NPH insulin-dependent diabetics undergoing cardiac catheterization. Circulation. 1984;70:788–92.
Levine GN, Bates ER, Blankenship JC, et al. 2011 ACCF/AHA/SCAI guideline for percutaneous coronary intervention. A report of the American College of Cardiology Foundation/American Heart Association Task Force on Practice Guidelines and the Society for Cardiovascular Angiography and Interventions. J Am Coll Cardiol. 2011;58:e44–122.
Hirsh J, Anand SS, Halperin JL, et al. Mechanism of action and pharmacology of unfractionated heparin. Arterioscler Thromb Vasc Biol. 2001;21:1094–6.
Handeland GF, Abildgaard U, Holm HA, et al. Dose adjusted heparin treatment of deep venous thrombosis: a comparison of unfractionated and low molecular weight heparin. Eur J Clin Pharmacol. 1990;39:107–12.
Hirsh J, Levine MN. Low molecular weight heparin. Blood. 1992;79:1–17.
Weitz JI. Low-molecular-weight heparins. N Engl J Med. 1997;337:688–98.
Boneu B, Caranobe C, Cadroy Y, et al. Pharmacokinetic studies of standard unfractionated heparin, and low molecular weight heparins in the rabbit. Semin Thromb Hemost. 1988;14:18–27.
Brophy DF, Wazny LD, Gehr TW, et al. The pharmacokinetics of subcutaneous enoxaparin in end-stage renal disease. Pharmacotherapy. 2001;21:169–74.
Becker RC, Spencer FA, Gibson M, et al. Influence of patient characteristics and renal function on factor Xa inhibition pharmacokinetics and pharmacodynamics after enoxaparin administration in non-ST-segment elevation acute coronary syndromes. Am Heart J. 2002;143:753–9.
Samama MM, Poller L. Contemporary laboratory monitoring of low molecular weight heparins. Clin Lab Med. 1995;15:119–23.
Boneu B, de Moerloose P. How and when to monitor a patient treated with ow molecular weight heparin. Semin Thromb Hemost. 2001;27:519–22.
Johnson EA, Kirkwood TB, Stirling Y, et al. Brozovic M. Four heparin preparations: anti-Xa potentiating effect of heparin after subcutaneous injection. Thromb Haemost. 1976;35:586–91.
Choay J, Petitou M, Lormeau JC, et al. Structure-activity relationship in heparin: a synthetic pentasaccharide with high affinity for antithrombin III and eliciting high anti-factor Xa activity. Biochem Biophys Res Commun. 1983;116:492–9.
Bara L, Samama M. Pharmacokinetics of low molecular weight heparins. Acta Chir Scand Suppl. 1988;543:65–72.
Bradbrook ID, Magnani HN, Moelker HC, et al. ORG 10172: a low molecular weight heparinoid anticoagulant with a long half-life in man. Br J Clin Pharmacol. 1987;23:667–75.
Antman EM, Cohen M, Radley D, et al. Assessment of the treatment effect of enoxaparin for unstable angina/non-Q-wave myocardial infarction. TIMI 11B-ESSENCE meta-analysis. Circulation. 1999;100:1602–8.
The Thrombolysis in Myocardial Infarction (TIMI) 11A Trial Investigators. Dose-ranging trial of enoxaparin for unstable angina: results of TIMI 11A. J Am Coll Cardiol. 1997;29:1474–82.
Bruno R, Baille P, Retout S, et al. Population pharmacokinetics and pharmacodynamics of enoxaparin in unstable angina and non-ST-segment elevation myocardial infarction. Br J Clin Pharmacol. 2003;56:407–14.
Warkentin TE, Roberts RS, Hirsh J, et al. An improved definition of immune heparininduced thrombocytopenia in postoperative orthopedic patients. Arch Intern Med. 2003;163:2518–24.
Greinacher A, Michels I, Mueller-Eckhardt C. Heparin-associated thrombocytopenia: the antibody is not heparin specific. Thromb Haemost. 1992;67:545–9.
Monreal M, Vinas L, Monreal L, et al. Heparin-related osteoporosis in rats. A comparative study between unfractioned heparin and a low-molecular-weight heparin. Haemostasis. 1990;20:204–7.
Racanelli A, Fareed J, Walenga JM, et al. Biochemical and pharmacologic studies on the protamine interactions with heparin, its fractions and fragments. Semin Thromb Hemost. 1985;11:176–89.
Wong GC, Giugliano RP, Antman EM. Use of low-molecular-weight heparins in the management of acute coronary artery syndromes and percutaneous coronary intervention. JAMA. 2003;289:331–42.
Klein W, Buchwald A, Hillis SE, et al. Comparison of low-molecular-weight heparin with unfractionated heparin acutely and with placebo for 6 weeks in the management of unstable coronary artery disease. Fragmin in unstable coronary artery disease study (FRIC). Circulation. 1997;96:61–8.
Fragmin During Instability in Coronary Artery Disease (FRISC) Study Group. Low-molecular-weight heparin during instability in coronary artery disease. Lancet. 1996;347:561–8.
FRAX.I.S. (FRAxiparine in Ischaemic Syndrome) Investigators. Comparison of two treatment durations (6 days and 14 days) of a low molecular weight heparin with a 6-day treatment of unfractionated heparin in the initial management of unstable angina or non-Q wave myocardial infarction. Eur Heart J. 1999;20:1553–62.
Ferguson JJ, Califf RM, Antman EM, et al. Enoxaparin vs. unfractionated heparin in high-risk patients with non-ST-segment elevation acute coronary syndromes managed with an intended early invasive strategy: primary results of the SYNERGY randomized trial. JAMA. 2004;292:45–54.
Cohen M, Demers C, Gurfinkel E, et al. A comparison of low-molecular-weight heparin with unfractionated heparin for unstable coronary artery disease. N Engl J Med. 1997;337:447–52.
Antman EM, McCabe CH, Gurfinkel EP, et al. Enoxaparin prevents death and cardiac ischemic events in unstable angina/non-Q-wave myocardial infarction. Results of the thrombolysis in myocardial infarction (TIMI) 11B trial. Circulation. 1999;100:1593–601.
Cohen M, Theroux P, Borzak S, et al. Randomized double-blind safety study of enoxaparin versus unfractionated heparin in patients with non-ST-segment elevation acute coronary syndromes treated with tirofiban and aspirin: the ACUTE II study. The Antithrombotic Combination Using Tirofiban and Enoxaparin. Am Heart J. 2002;144:470–7.
Goodman SG, Fitchett D, Armstrong PW, et al. Randomized evaluation of the safety and efficacy of enoxaparin versus unfractionated heparin in high-risk patients with non-ST-segment elevation acute coronary syndromes receiving the glycoprotein IIb/IIIa inhibitor eptifibatide. Circulation. 2003;107:238–44.
Blazing MA, de Lemos JA, White HD, et al. Safety and efficacy of enoxaparin vs. unfractionated heparin in patients with non-ST-segment elevation acute coronary syndromes who receive tirofiban and aspirin: a randomized controlled trial. JAMA. 2004;292:55–64.
Antman EM. The search for replacements for unfractionated heparin. Circulation. 2001;103:2310–4.
Petersen JL, Mahaffey KW, Hasselblad V, et al. Efficacy and bleeding complications among patients randomized to enoxaparin or unfractionated heparin for antithrombins therapy in non-ST-Segment elevation acute coronary syndromes: a systematic overview. JAMA. 2004;292:89–96.
The ASSENT‐3 Investigators. Efficacy and safety of tenecteplase in combination with enoxaparin, abciximab, or unfractionated heparin: the ASSENT‐3 randomised trial in acute myocardial infarction. Lancet. 2001;358:605–13.
Wallentin L, Goldstein P, Armstrong PW, et al. Efficacy and safety of tenecteplase in combination with the low-molecular-weight heparin enoxaparin or unfractionated heparin in the prehospital setting: the Assessment of the Safety and Efficacy of a New Thrombolytic Regimen (ASSENT)-3 PLUS randomized trial in acute myocardial infarction. Circulation. 2003;108:135–42.
Giraldez RR, Nicolau JC, Corbalan R, et al. Enoxaparin is superior to unfractionated heparin in patients with ST elevation myocardial infarction undergoing fibrinolysis regardless of the choice of lytic: an ExTRACT-TIMI 25 analysis. Eur Heart J. 2007;28:1566–73.
White HD, Braunwald E, Murphy SA, et al. Enoxaparin vs. unfractionated heparin with fibrinolysis for ST-elevation myocardial infarction in elderly and younger patients: results from ExTRACT-TIMI 25. Eur Heart J. 2007;28:1066–71.
Montalescot G, White HD, Gallo R, et al. STEEPLE Investigators. Enoxaparin versus unfractionated heparin in elective percutaneous coronary intervention. N Engl J Med. 2006;355:1058–60.
Antman EM, Louwerenburg HW, Baars HF, et al. Enoxaparin as adjunctive antithrombin therapy for ST-elevation myocardial infarction: results of the ENTIRE-Thrombolysis in Myocardial Infarction (TIMI) 23 Trial. Circulation 105:1642–9. Erratum in. Circulation. 2002;105:2799.
Gibson CM, Murphy SA, Montalescot G, et al. Percutaneous coronary intervention in patients receiving enoxaparin or unfractionated heparin after fibrinolytic therapy for ST-segment elevation myocardial infarction in the ExTRACT-TIMI 25 trial. J Am Coll Cardiol. 2007;49:2238–46.
Levine MN, Raskob G, Beyth RJ, et al. Hemorrhagic complications of anticoagulant treatment: The Seventh ACCP Conference on Antithrombotic and Thrombolytic Therapy. Chest. 2004;126:287S–310.
Eisert WG, Hauel N, Stangier J, et al. Dabigatran: an oral novel potent reversible nonpeptide inhibitor of thrombin. Arterioscler Thromb Vasc Biol. 2010;30:1885–9.
Weitz JI. Factor Xa and thrombin as targets for new oral anticoagulants. Thromb Res. 2011;127:S5–12.
Bates SM, Weitz JI. Direct thrombin inhibitors for treatment of arterial thrombosis: potential differences between bivalirudin and hirudin. Am J Cardiol. 1998;82:12P–8.
Xiao Z, Theroux P. Platelet activation with unfractionated heparin at therapeutic concentrations and comparisons with a low-molecular-weight heparin and with a direct thrombin inhibitor. Circulation. 1998;97:251–6.
Sorensen B, Ingerslev J. A direct thrombin inhibitor studied by dynamic whole blood clot formation. Haemostatic response to ex-vivo addition of recombinant factor VIIa or activated prothrombin complex concentrate. Thromb Haemost. 2006;96:446–53.
Wallis RB. Hirudins: from leeches to man. Semin Thromb Hemost. 1996;22:185–96.
OASIS-2 Investigators. Effects of recombinant hirudin (lepirudin) compared with heparin on death, myocardial infarction, refractory angina, and revascularisation procedures in patients with acute myocardial ischaemia without ST elevation: A randomised trial. Lancet. 1999;353:429–38.
Garcia DA, Baglin TP, Weitz JI, et al. Parenteral anticoagulants: Antithrombotic Therapy and Prevention of Thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines. Chest. 2012;141:e24S–43.
Lefevre G, Duval M, Gauron S, et al. Effect of renal impairment on the pharmacokinetics and pharmacodynamics of desirudin. Clin Pharmacol Ther. 1997;62:50–9.
Banner DW, Hadvary P. Inhibitor binding to thrombin: x-ray crystallographic studies. Adv Exp Med Biol. 1993;340:27–33.
Hursting MJ, Alford KL, Becker JC, et al. Novastan (brand of argatroban): a small molecule, direct thrombin inhibitor. Semin Thromb Hemost. 1997;23:503–16.
Lewis BE, Wallis DE, Leya F, et al. Argatroban-915 Investigators. Argatroban anticoagulation in patients with heparin-induced thrombocytopenia. Arch Intern Med. 2003;163:1849–56.
Swan SK, Hursting MJ. The pharmacokinetics and pharmacodynamics of argatroban: effects of age, gender, and hepatic or renal dysfunction. Pharmacotherapy. 2000;20:318–29.
Maraganore JM, Bourdon P, Jablonski J, et al. Design and characterization of hirulogs: a novel class of bivalent peptide inhibitors of thrombin. Biochemistry. 1990;29:7095–101.
Skrzypczak-Jankun E, Carperos VE, Ravichandran KG, et al. Structure of the hirugen and hirulog 1 complexes of alpha-thrombin. J Mol Biol. 1991;221:1379–93.
Witting JI, Bourdon P, Brezniak DV, et al. Thrombinspecific inhibition by and slow cleavage of hirulog-1. Biochem J. 1992;283:737–43.
Fox I, Dawson A, Loynds P, et al. Anticoagulant activity of Hirulog, a direct thrombin inhibitor, in humans. Thromb Haemost. 1993;69:157–63.
Robson R, White H, Aylward P, et al. Bivalirudin pharmacokinetics and pharmacodynamics: effect of renal function, dose, and gender. Clin Pharmacol Ther. 2002;71:433–9.
Chew DP, Bhatt DL, Kimball W, et al. Bivalirudin provides increasing benefit with decreasing renal function: a meta-analysis of randomized trials. Am J Cardiol. 2003;92:919–23.
Stone G, McLaurin BT, Cox DA, et al. ACUITY Investigators. Bivalirudin for patients with acute coronary syndromes. N Engl J Med. 2006;355:2203–16.
Kastrati A, Neumann FJ, Schulz S, et al. Abciximab and heparin versus bivalirudin for non-ST-elevation myocardial infarction. ISAR REACT 4 trial. New Engl J Med. 2011;365:1980–9.
Stone GW, Witzenbichler B, Guagliumi G, et al. Bivalirudin during primary PCI in acute myocardial infarction. N Engl J Med. 2008;358:2218–30.
Wasowicz M, Vegas A, Borger MA, et al. Bivalirudin anticoagulation for cardiopulmonary bypass in a patient with heparin-induced thrombocytopenia. Can J Anaesth. 2005;52:1093–8.
Mahaffey KW, Lewis BE, Wildermann NM, et al. The anticoagulant therapy with bivalirudin to assist in the performance of percutaneous coronary intervention in patients with heparin-induced thrombocytopenia (ATBAT) study: main results. J Invasive Cardiol. 2003;15:611–6.
Lincoff AM, Bittl JA, Harrington RA, et al. Bivalirudin and provisional glycoprotein IIb/IIIa blockade compared with heparin and planned glycoprotein IIb/IIIa blockade during percutaneous coronary intervention: REPLACE-2 randomized trial. JAMA. 2003;289:853–63.
Mehran R, Lansky AJ, Witzenbichler B, et al. Bivalirudin in patients undergoing primary angioplasty for acute myocardial infarction (HORIZONS-AMI): 1-year results of a randomised controlled trial. Lancet. 2009;374:1149–59.
Stone GW, Witzenbichler B, Guagliumi G, et al. Heparin plus a glycoprotein IIb/IIIa inhibitor versus bivalirudin monotherapy and paclitaxel-eluting stents versus bare-metal stents in acute myocardial infarction (HORIZONS-AMI): final 3-year results from a multicentre, randomised controlled trial. Lancet. 2011;377:2193–204.
Dangas GD, Claessen BE, Mehran R, Brener S, et al. Clinical outcomes following stent thrombosis occurring in-hospital versus out-of-hospital: results from the HORIZONS-AMI (Harmonizing Outcomes with Revascularization and Stents in Acute Myocardial Infarction) trial. J Am Coll Cardiol. 2012;59:1752–9.
Dangas G, Mehran R, Guagliumi G, et al. Role of clopidogrel loading dose in patients with ST-segment elevation myocardial infarction undergoing primary angioplasty: results from the HORIZONS-AMI (harmonizing outcomes with revascularization and stents in acute myocardial infarction) trial. J Am Coll Cardiol. 2009;54:1438–46.
Boneu B, Necciari J, Cariou R, et al. Pharmacokinetics and tolerance of the natural pentasaccharide (SR90107/Org31540) with high affinity to antithrombin III in man. Thromb Haemost. 1995;74:1468–73.
Donat F, Duret JP, Santoni A, et al. The pharmacokinetics of fondaparinux sodium in healthy volunteers. Clin Pharmacokinet. 2002;41:1–9.
Lieu C, Shi J, Donat F, et al. Fondaparinux sodium is not metabolised in mammalian liver fractions and does not inhibit cytochrome P450-mediated metabolism of concomitant drugs. Clin Pharmacokinet. 2002;41:19–26.
Bassand JP, Hamm CW, Ardissino D, et al. Guidelines for the diagnosis and treatment of non-ST-segment elevation acute coronary syndromes. Eur Heart J. 2007;28:1598–660.
Paolucci F, Clavies MC, Donat F, et al. Fondaparinux sodium mechanism of action: identification of specific binding to purified and human plasma-derived proteins. Clin Pharmacokinet. 2002;41:11–8.
Savi P, Chong BH, Greinacher A, et al. Effect of fondaparinux on platelet activation in the presence of heparin-dependent antibodies: a blinded comparative multicenter study with unfractionated heparin. Blood. 2005;105:139–44.
Kuo KH, Kovacs MJ. Fondaparinux: a potential new therapy for HIT. Hematology. 2005;10:271–5.
Parody R, Oliver A, Souto JC, et al. Fondaparinux (ARIXTRA) as an alternative antithrombotic prophylaxis when there is hypersensitivity to low molecular weight and unfractionated heparins. Haematologica. 2003;88:ECR32.
Matziolis G, Perka C, Disch A, et al. Effects of fondaparinux compared with dalteparin, enoxaparin and unfractionated heparin on human osteoblasts. Calcif Tissue Int. 2003;73:370–9.
Lagrange F, Vergnes C, Brun JL, et al. Absence of placental transfer of pentasaccharide (Fondaparinux, Arixtra) in the dually perfused human cotyledon in vitro. Thromb Haemost. 2002;87:831–5.
Simoons ML, Bobbink IW, Boland J, et al. A dose-finding study of fondaparinux in patients with non-ST-segment elevation acute coronary syndromes: the Pentasaccharide in Unstable Angina (PENTUA) Study. J Am Coll Cardiol. 2004;43:2183–90.
Bijsterveld NR, Moons AH, Boekholdt SM, et al. Ability of recombinant factor VIIa to reverse the anticoagulant effect of the pentasaccharide fondaparinux in healthy volunteers. Circulation. 2002;106:2550–4.
Yusuf S, Mehta SR, Chrolavicius S, et al. Comparison of fondaparinux and enoxaparin in acute coronary syndromes. N Engl J Med. 2006;354:1464–76.
Steg PG, Mehta S, Jolly S, et al. Fondaparinux with UnfracTionated heparin dUring Revascularization in Acute coronary syndromes (FUTURA/OASIS 8): a randomized trial of intravenous unfractionated heparin during percutaneous coronary intervention in patients with non-ST-segment elevation acute coronary syndromes initially treated with fondaparinux. Am Heart J. 2010;160:1029–34.
Yusuf S, Mehta SR, Chrolavicius S, et al. Effects of fondaparinux on mortality and reinfarction in patients with acute ST-segment elevation myocardial infarction: the OASIS-6 randomized trial. JAMA. 2006;295:1519–30.
Hinder M, Frick A, Jordaan P, et al. Direct and rapid inhibition of factor Xa by otamixaban: a pharmacokinetic and pharmacodynamic investigation in patients with coronary artery disease. Clin Pharmacol Ther. 2006;80:691–702.
Cohen M, Bhatt DL, Alexander JH, et al. Randomized, double-blind, dose-ranging study of otamixaban, a novel, parenteral, short-acting direct factor Xa inhibitor, in percutaneous coronary intervention: the SEPIA-PCI trial. Circulation. 2007;115:2642–51.
Sabatine MS, Antman EM, Widimsky P, et al. Otamixaban for the treatment of patients with non-ST-elevation acute coronary syndromes (SEPIA-ACS1 TIMI 42): a randomised, double-blind, active-controlled, phase 2 trial. Lancet. 2009;374:787–95.
Steg PG, Mehta SR, Pollack Jr CV, et al. Design and rationale of the treatment of acute coronary syndromes with otamixaban trial: a double-blind triple-dummy 2-stage randomized trial comparing otamixaban to unfractionated heparin and eptifibatide in non-ST-segment elevation acute coronary syndromes with a planned early invasive strategy. Am Heart J. 2012;164:817–24.
Angiolillo DJ, Ueno M, Goto S. Basic principles of platelet biology and clinical implications. Circ J. 2010;74:597–607.
Eikelboom JW, Weitz JI, Budaj A, et al. Clopidogrel does not suppress blood markers of coagulation activation in aspirin-treated patients with non-ST-elevation acute coronary syndromes. Eur Heart J. 2002;23:1771–9.
Hurlen M, Abdelnoor M, Smith P, et al. Warfarin, aspirin, or both after myocardial infarction. N Engl J Med. 2002;347:969–74.
Herlitz J, Holm J, Peterson M, et al. Effect of fixed low-dose warfarin added to aspirin in the long term after acute myocardial infarction: the LoWASA Study. Eur Heart J. 2004;25:232–9.
Orford JL, Fasseas P, Melby S, et al. Safety and efficacy of aspirin, clopidogrel, and warfarin after coronary stent placement in patients with an indication for anticoagulation. Am Heart J. 2004;1473:463–7.
Karjalainen PP, Porela P, Ylitalo A, et al. Safety and efficacy of combined antiplatelet-warfarin therapy after coronary stenting. Eur Heart J. 2007;286:726–32.
Khurram Z, Chou E, Minutello R, et al. Combination therapy with aspirin, clopidogrel and warfarin following coronary stenting is associated with a significant risk of bleeding. J Invasive Cardiol. 2006;184:162–4.
Becker RC. Antithrombotic therapy after myocardial infarction. N Engl J Med. 2002;347:1019–22.
Connolly SJ, Ezekowitz MD, Yusuf S, et al. Dabigatran versus warfarin in patients with atrial fibrillation. N Engl J Med. 2009;361:1139–51.
Patel MR, Mahaffey KW, Garg J, et al. Rivaroxaban versus warfarin in nonvalvular atrial fibrillation. N Engl J Med. 2011;365:883–9.
Connolly SJ, Eikelboom J, Joyner C, et al. Apixaban in patients with atrial fibrillation. N Engl J Med. 2011;364:806–17.
Stangier J, Rathgen K, Stähle H, et al. Influence of renal impairment on the pharmacokinetics and pharmacodynamics of oral dabigatran etexilate: an open-label, parallel-group, single-centre study. Clin Pharmacokinet. 2010;49:259–68.
Schulman S, Crowther MA. How I treat with anticoagulants in 2012: new and old anticoagulants, and when and how to switch. Blood. 2012;119:3016–23.
Spyropoulos AC, Douketis JD. How I treat anticoagulated patients undergoing an elective procedure or surgery. Blood. 2012;120:2954–62.
Wallentin L, Wilcox RG, Weaver WD, et al. ESTEEM Investigators. Oral ximelagatran for secondary prophylaxis after myocardial infarction: the ESTEEM randomised controlled trial. Lancet. 2003;362:789–97.
Sorbera LA, Bozzo J, Castaner J. Dabigatran/dabigatran etexilate. Drugs Fut. 2005;30:877–85.
Stangier J, Eriksson BI, Dahl OE, et al. Pharmacokinetic profile of the oral direct thrombin inhibitor dabigatran etexilate in healthy volunteers and patients undergoing total hip replacement. J Clin Pharmacol. 2005;45:555–63.
Eriksson BI, Dahl OE, Ahnfelt L, et al. Dose escalating safety study of a new oral direct thrombin inhibitor, dabigatran etexilate, in patients undergoing total hip replacement: BISTRO I. J Thromb Haemost. 2004;2:1573–80.
De Caterina R, Husted S, Wallentin L. New oral anticoagulants in atrial fibrillation and acute coronary syndromes: ESC Working Group on Thrombosis-Task Force on Anticoagulants in Heart Disease position paper. J Am Coll Cardiol. 2012;59:1413–25.
Eerenberg ES, Kamphuisen PW, Sijpkens MK, et al. Reversal of rivaroxaban and dabigatran by prothrombin complex concentrate: a randomized, placebo-controlled, crossover study in healthy subjects. Circulation. 2011;124:1573–9.
Oldgren J, Budaj A, Granger CB, et al. Dabigatran vs. placebo in patients with acute coronary syndromes on dual antiplatelet therapy: a randomized, double-blind, phase II trial. Eur Heart J. 2011;32:2781–9.
Jiang J, Hu Y, Zhang J, et al. Safety, pharmacokinetics and pharmacodynamics of single doses of rivaroxaban -an oral, direct factor Xa inhibitor- in elderly Chinese subjects. Thromb Haemost. 2010;103:234–41.
Steg PG, Mehta SR, Jukema JW, et al. RUBY-1: a randomized, double-blind, placebo-controlled trial of the safety and tolerability of the novel oral factor Xa inhibitor darexaban (YM150) following acute coronary syndrome. Eur Heart J. 2011;32:2541–54.
Alexander JH, Becker RC, Bhatt DL, et al. Apixaban, an oral, direct, selective factor Xa inhibitor, in combination with antiplatelet therapy after acute coronary syndrome: results of the Apixaban for Prevention of Acute Ischemic and Safety Events (APPRAISE) trial. Circulation. 2009;119:2877–85.
Alexander JH, Lopes RD, James S, et al. The APPRAISE 2 Investigators. Apixaban with antiplatelet therapy after acute coronary syndrome. N Engl J Med. 2011;365:699–708.
Mega JL, Braunwald E, Mohanavelu S, et al. Rivaroxaban versus placebo in patients with acute coronary syndromes (ATLAS ACSTIMI 46): a randomised, double-blind, phase II trial. Lancet. 2009;374:29–38.
Gibson CM, Mega JL, Burton P, et al. Rationale and design of the Anti-Xa therapy to lower cardiovascular events in addition to standard therapy in subjects with acute coronary syndrome-thrombolysis in myocardial infarction 51 (ATLAS-ACS 2 TIMI 51) trial: a randomized, double-blind, placebo-controlled study to evaluate the efficacy and safety of rivaroxaban in subjects with acute coronary syndrome. Am Heart J. 2011;161:815–21.
Mega JL, Braunwald E, Wiviott SD, et al. Rivaroxaban in patients with a recent acute coronary syndrome. N Engl J Med. 2012;366:9–19.
Becker RC, Rusconi C, Sullenger B. Nucleic acid aptamers in therapeutic anticoagulation. Technology, development, and clinical application. Thromb Haemost. 2005;93:1014–20.
Becker RC, Povsic TJ, Cohen MG, et al. Nucleic acid aptamers as antithrombotic agents: opportunities in extracellular therapeutics. Thromb Haemost. 2010;103:586–95.
Povsic T, Sullenger B, Zelenkofske S, et al. Translating nucleic acid aptamers to antithrombotic drugs in cardiovascular medicine. J Cardiovasc Transl Res. 2011;3:704–16.
Povsic TJ, Cohen MG, Chan MY, et al. Dose selection for a direct and selective factor IXa inhibitor and its complementary reversal agent: translating pharmacokinetic and pharmacodynamic properties of the REG1 system to clinical trial design. J Thromb Thrombolysis. 2011;32:21–31.
Dyke CK, Steinhubl SR, Kleiman NS, et al. First-in-human experience of an antidote-controlled anticoagulant using RNA aptamer technology: a phase 1a pharmacodynamic evaluation of a drug-antidote pair for the controlled regulation of factor IXa activity. Circulation. 2006;114:2490–7.
Chan MY, Cohen MG, Dyke CK, et al. Phase 1b randomized study of antidote-controlled modulation of factor IXa activity in patients with stable coronary artery disease. Circulation. 2008;117:2865–74.
Chan MY, Rusconi CP, Alexander JH, et al. A randomized, repeat-dose, pharmacodynamic and safety study of an antidotecontrolled factor IXa inhibitor. J Thromb Haemost. 2008;6:789–96.
Cohen MG, Purdy DA, Rossi JS, et al. First clinical application of an actively reversible direct factor IXa inhibitor as an anticoagulation strategy in patients undergoing percutaneous intervention. Circulation. 2010;122:614–22.
Povsic TJ, Cohen MG, Mehran R, et al. A randomized, partially-blinded, multicenter, activecontrolled, dose-ranging study assessing the safety, efficacy, and pharmacodynamics of the REG1 anticoagulation system in patients with acute coronary syndromes: design and rationale of the RADAR phase IIb trial. Am Heart J. 2011;161:261–8.
Povsic TJ, Wargin WA, Alexander JH, et al. Pegnivacogin results in near complete FIX inhibition in acute coronary syndrome patients: RADAR pharmacokinetic and pharmacodynamic substudy. Eur Heart J. 2011;32:2412–9.
Esmon CT. Crosstalk between inflammation and thrombosis. Maturitas. 2004;47:305–14.
Coughlin SR. Protease-activated receptors in haemostasis, thrombosis and vascular biology. J Thromb Haemost. 2005;3:1800–14.
Ruf W, Dorfleutner A, Riewald M. Specificity of coagulation factor signalling. J Thromb Haemost. 2003;1:1495–503.
Morrow DA, Braunwald E, Bonaca MP, et al. Vorapaxar in the secondary prevention of atherothrombotic events. N Engl J Med. 2012;366:1404–13.
Tricoci P, Huang Z, Held C, et al. Thrombin-receptor antagonist vorapaxar in acute coronary syndromes. N Engl J Med. 2012;366:20–33.
Collin N, Assumpção TC, Mizurini DM, et al. Lufaxin, a Novel Factor Xa Inhibitor From the Salivary Gland of the Sand Fly Lutzomyia longipalpis Blocks Protease-Activated Receptor 2 Activation and Inhibits Inflammation and Thrombosis In Vivo. Arterioscler Thromb Vasc Biol. 2012;32:2185–98.
Acknowledgements
Ana Muñiz-Lozano is recipient of a training grant from the Spanish Society of Cardiology (“Beca de la Sección de Cardiopatía Isquémica para Formación e Investigación Post Residencia en el Extranjero” – Sociedad Española de Cardiología).
Disclosures
Dominick J. Angiolillo has received payment as an individual for: (a) Consulting fee or honorarium from Bristol Myers Squibb, Sanofi-Aventis, Eli Lilly, Daiichi Sankyo, The Medicines Company, AstraZeneca, Merck, Evolva, Abbott Vascular and PLx Pharma; (b) Participation in review activities from Johnson & Johnson, St. Jude, and Sunovion. Institutional payments for grants from Bristol Myers Squibb, Sanofi-Aventis, Glaxo Smith Kline, Otsuka, Eli Lilly, Daiichi Sankyo, The Medicines Company, AstraZeneca, Evolva; and has other financial relationships with Esther and King Biomedical Research Grant.
Ana Muñiz-Lozano: has no conflict of interest to report.
Fabiana Rollini: has no conflict of interest to report.
Francesco Franchi: has no conflict of interest to report.
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Muñiz-Lozano, A., Rollini, F., Franchi, F., Angiolillo, D.J. (2014). Anticoagulation Therapy. Heparins, Factor II and Factor Xa Inhibitors. In: Avanzas, P., Clemmensen, P. (eds) Pharmacological Treatment of Acute Coronary Syndromes. Current Cardiovascular Therapy. Springer, London. https://doi.org/10.1007/978-1-4471-5424-2_3
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