Abstract
An important property of all biological membranes is that they are selectively permeable to a variety of cations and anions (including the principal cellular ions H+, Na+, K+ and Cl-), so that the different ions tend to move down their concentration gradients through the membrane at different rates. These two characteristics, selective permeability and ionic concentration gradients, lead to a difference in electric potential between the inside and the outside of a cell.
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References
Ahmed S, Booth IR (1981) Quantitative measurements of the proton-motive force and its relation to steady state lactose accumulation in Escherichia coli .Biochem J 200:573–581
Bakker EP (1982) Membrane potential in a potassium transport-negative mutant of Escherichia coli K-12. Biochim Biophys Acta 681:474–483
Bendall MJ, Ebrahim S, Finch RG, Slack RCB, Towner KJ (1986) The effect of an antibiotic policy on bacterial resistance in patients in geriatric wards. J Med 60:849–854
Booth JR, Mitchell WJ, Hamilton WA (1979) Quantitative analysis of proton-linked transport systems. Biochem J 182:687–696
Eddy A (1989) Use of carbocyanine dyes to assay membrane potential of mouse ascites tumour cells. Meth Enzymol 172:95–101
Eriok BJS, Webster DA (1990) Respiratory-driven Na+ electrical potential in the bacterium Vitreoscilla. Biochemistry 29:4734–4739
Hargittai PT, Youmans SJ, Lieberman EM (1991) Determination of the membrane potential of cultured mammalian Schwann cells and its sensitivity to potassium using a thiocarbocyanine fluorescent dye. Glia 4:611–616
Kamo N, Muratsuga M, Hongoh R, Kobatake Y (1979) Membrane potential of mitochondria measured with an electrode sensitive to tetraphenyl phosphonium and relationship between proton electrochemical potential and phosphorylation potential in steady state. J Membr Biol 49:105–121
Kaprelyants AS, Kell DB (1992) Rapid assessment of bacterial viability and vitality using Rhodamine 123 and flow cytometry. J Appl Bacteriol 72:410–422
Kessel D, Beck WT, Kukuruga D, Schulz V (1991) Characterization of multidrug resistance by fluorescent dyes. Cancer Res 51:4665–4670
Klugman KP, Koornhof HJ (1988) Bacteremic pneumonia caused by penicillin-resistant pneumococci. N Engl J Med 318:123–124
Lambert HP (1988) Clinical impact of drug resistance. J Hosp Inf 2 (Suppl A): 135–141
Lolkema JS, Hellingwerf KJ, Konings WN (1982) The effect of “probe binding” on the quantitative determination of the proton-motive force in bacteria. Biochim Biophys Acta 681:85–94
Matsuyama T (1984) Staining of living bacteria with rhodamine 123. FEMS Microbiol Lett 21:153–157
Muratsuga M, Kamo N, Kurihara K, Kobatake Y (1977) Selective electrode for diebenzyl diemethyl ammonium cation as indicator of the membrane potential in biological systems. Biochim Biophys Acta 464:613–619
Oyama Y, Chikahisa L, Tomiyoshi F, Hayashi II (1991) Cytotoxic action of triphenyltin on mouse thymocytes: a flow-cytometric study using fluorescent dyes for membrane potential and intracellular Ca2+. Jpn J Pharmacol 57:419–424
Pallares R, Gudiol F, Linares J et al. (1987) Risk factors and response to antibiotic therapy in adults with bacteremic pneumonia caused by penicillin-resistant pneumococci. N Engl J Med 317:18–22
Pena A, Uribe S, Pardo JP, Barbolla M (1984) The use of a cyanine dye in measuring membrane potential in yeast. Arch Biochem Biophys 231:217–225
Petit PX, O’Connor JE, Grunwald D, Brown SC (1990) Analysis of the membrane potential of rat and mouse liver mitochondria by flow cytometry and possible applications. Eur J Biochem 194:389–397
Philo R, Eddy AA (1978) The membrane potential of mouse ascites - tumour cells studied with the fluorescent probe 3,3-dipropyloxadicarbocyanine. Amplitude of the depolarization caused by amino acids. Biochem J 174:801–810
Piddock LJV (1990) Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J Appl Bacteriol 68:307–318
Ronot X, Benel L, Adolphe M, Mounlou J (1986) Mitochondria analysis in living cells: the use of Rhodamine 123 and flow cytometry. Biol Cell 57:1–8
Schuldiner S, Kaback HR (1975) Membrane potential and active transport in membrane vesicles from Escherichia coli. Biochemistry 14:5451–5416
Shalit I, Berger SA, Gorea A, Frimerman H (1989) Widespread quinolone resistance among methicillin-resistant Staphylococcus aureus: isolates in a general hospital. Antimicrob Agents Chemother 33:593–594
Shinbo T, Kamo N, Kurihara K, Kobatake (1978) A PVC based electrode sensitive to DDA as a device for monitoring the membrane potential in biological systems. Arch Biochem Biophys 187:414–419
Shinomiya N, Tsuru S, Katsura Y, Sekiguchi I, Suzuki M, Nomoto K (1992) Increased mitochondrial uptake of Rhodamine 123 by CDDP treatment. Exp Cell Res 198:159–163
Sims J, Waggoner AS, Wang C, Hoffman JF (1974) Studies of the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry 13:3315–3329
Skowronek P, Krummeck G, Haferkump O, Rodel G (1990) Flow cytometry as a tool to discriminate respiratory competent and respiratory deficient yeast cells. Curr Genet 18: 265–267
Stokes EJ, Ridgeway GL (1987) Clinical microbiology, 6th edn. Edward Arnold, London
Zaritsky A, Kihara M, MacNab RM (1981) Measurement of membrane potential in Bacillus subtilis: a comparison of lipophilic cations, rubidium ion, and a cyanine dye as probes. J Membr Biol 63:215–231
Zilberstein D, Schudliner S, Padan E (1979) Proton electrochemical gradient in Escherichia coli cells and its relation to active transport of lactose. Biochemistry 18:669–673
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© 1993 Springer-Verlag London
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Mason, D., Allman, R., Lloyd, D. (1993). Uses of Membrane Potential Sensitive Dyes with Bacteria. In: Lloyd, D. (eds) Flow Cytometry in Microbiology. Springer, London. https://doi.org/10.1007/978-1-4471-2017-9_5
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DOI: https://doi.org/10.1007/978-1-4471-2017-9_5
Publisher Name: Springer, London
Print ISBN: 978-1-4471-2019-3
Online ISBN: 978-1-4471-2017-9
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