Abstract
The world of microorganisms provides a playground both for those fascinated by the prospects of understanding the nature of life, and for those interested in solving everyday problems arising from microbial activities. Both fundamental studies and applied aspects of microbiology have become “illuminated” in recent years by the techniques of flow cytometry (Boye and Løbner-Olesen 1990). It is, however, somewhat disappointing, considering the truly remarkable demonstrations of the usefulness and power of these approaches, that only a rather slow adoption has ensued. The reasons for this are not hard to find, and do not in any way detract from the great potential of flow cytometry as amply illustrated by existing literature. Cost may become less of a constraint as suitable low-power lasers become available (Shapiro 1985). It is also worth remembering that a high-pressure mercury arc lamp can give results as good as (or better than) a 5 W argonion laser in many applications (Peters 1979).
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References
Agar DW, Bailey JE (1982) Cell cycle operation during batch growth of fission yeast populations. Cytometry 3:123
Alderete JF, Kasmala L, Metcalfe E, Garza GE (1986) Phenotypic variation and diversity among Trichomonas vaginalis isolates and correlation of phenotype with trichomonal virulence determinants. Infect Immunol 53:285
Allman R, Hann AC, Phillips AP, Martin KL, Lloyd D (1990) Growth of Azotobacter vinelandii with correlation of Counter cell size, flow cytometric parameters and ultrastructure. Cytometry 11:822–831
Amy PS, Morita RY (1983) Starvation survival patterns of 16 freshly isolated open ocean bacteria. Appl Environ Microbiol 45:1109–1115
Arndt-Jovin DJ, Jovin TM (1974) Computer-controlled cell (particle) analyser and separator: use of light scattering. FEBS Lett 44:247
Berridge MJ (1989) Cell signalling through cytoplasmic calcium oscillators. In: Goldbeter A (ed) Academic Press, London, pp. 449–460
Bisset KA (1952) Bacteria. Livingstone, Edinburgh
Boye E, Løbner-Olesen A (1990) Flow cytometry: illuminating microbiology. The New Biologist 2:119–125
Boye E, Løbner-Olesen A, Skarstad K (1988) Timing of chromosomal replication in Escherichia coli. Biochim Biophys Acta 951:359–364
Chisholm SW, Olson RJ, Zettler ER, Goericke R, Waterbury JB, Welschmeyer NA (1988) A novel free-living prochlorophyte abundant in the oceanic euphotic zone. Nature 334:340–343
Cooper S (1991) Bacterial growth and division. Academic Press, London
Coulson PB, Tyndall R (1978) Quantitation by flow microfluorimetry of total cellular DNA in Acanthamoeba. J Histochem Cytochem 26:713–718
Crissman H, Tobey RA (1974) Cell cycle analysis in 20 minutes. Science 184:1297–1298
Das J, Busse HG (1991) Analysis of the dynamics of relaxation type oscillation in glycolysis of yeast extracts. Biophys J 60:369–379
Dennis K, Sricnc F, Bailey JE (1983) Flow cytomctric analysis of plasmid hctcrogeneity in Escherichia coli populations.. Biotechnol Bioeng 25:2485–2489
Dow CS, Whittenbury R, Carr NG (1983) The “shut down” or “growth precursor cell” - an adaptation for survival in a potentially hostile environment. Symp Soc Gen Microbiol 34:187–247, Cambridge University Press, Cambridge
Edwards C, McCann RJ (1981) Differential effects of inhibitors on respiratory activity of synchronous cultures of Bacillus subtilis prepared by continuous-flow centrifugation. J Gen Microbiol 125:47–53
Eisert WG, Ostertag R, Niemann EG (1975) Simple flow microphotometer for rapid cell population analysis. Rev Sci Instrum 46:1021
Falchuk KH, Krishan A, Vallee BL (1975) DNA distribution in the cell cycle of Euglena gracilis. Cytofluorometry of zinc deficient cells. Biochemistry 14:3439
Fazel-Madjlessi J, Bailey JE (1979) Analysis of fermentation process using flow microfluorimetry: single-parameter observations of batch bacterial growth. Biotech Bioeng 21:1995–2010
Ferry RM, Farr Jr LE, Hartman MG (1949) The preparation and measurement of the concentration of dilute bacterial aerosols. Chern Rev 44:389–395
Goldbeter A (1990) Rythmes et chaos dans les systemes biochimique et cellulaires. Masson, Paris
Hayworth MF, Papo J (1989) Use of two-colour flow cytometry to assess killing of Giardia muris trophozoites by antibody and complement. Parasitology 99:199–203
Hercher M, Mueller W, Shapiro HM (1979) Detection and discrimination of individual viruses by flow cytometry. J Histochem Cytochem 27:350–352
Hill EC, Genner C (1981) Avoidance of microbial infection and corrosion in slow-speed diesel engines by improved design of the crankcase oil system. Tribology Int 8:67–74
Hutter KJ, Eipel HE (1978) Flow cytometric determinations of cellular substances in algae, bacteria, moulds and yeasts. Anton van Leeuw 44:269
Hutter KJ, Eipel HE (1979) Microbial determination by flow cytometry. J Gen Microbiol 113:369–375
Hutter KJ, Oldiges H (1980) Alterations of proliferating microorganisms by flow cytometric measurements after heavy metal intoxication. Ecotoxicol Environ Safety 4:57
Hutter KJ, Goehde W, Emeis CC (1975a) Investigation about the synthesis of DNA, RNA and proteins of selected populations of microorganisms by cytophotometry and pulsecytophotometry. I. Methodical investigations about appropriate fluorescence-dyes and staining procedures. Chern Mikrobiol Technol Lebensum 4:29–32
Hutter KJ, Otto F, Emeis CC (1975b) Investigations about the synthesis of DNA, RNA and proteins of selected populations of microorganisms by cytophotometry and pulsecytophotometry. II. Synthesis of DNA, RNA and proteins of yeast of the species Saccharomyces during the vegetative growth. Chern Mikrobiol Technol Lebensum 4:75–80
Hutter KJ, Eipel HE, Hettwer H (1978) Rapid determination of the purity of yeast cultures by flow cytometry. European J Appl Microbiol Biotechnol 5:109–112
Jackson PR, Winkler DG, Kimzey SL, Fisher FM Jr (1977) Cytofluorograf detection of Plasmodium yoelii, Trypanosoma gambiense, and Trypanosoma equiperdum by laser excited fluorescence and stained rodent blood. J Parasitol 63:593–598
Kamentsky LA, Melamed MR, Derman H (1965) Spectrophotometer: new instrument for ultrarapid cell analysis. Science 150:630–631
Lewis DL, Gattie DK (1991) The ecology of quiescent microbes. Am Soc Microbiol 57:27–32
Lloyd D (1992) Intracellular timekeeping: epigenetic oscillations reveal functions of an ultradian clock. In: Lloyd D, Rossi ER (eds) Ultradian rhythms in life processess: A fundamental inquiry into chronobiology, Springer, London, pp 5–21
Lloyd D, Stupfel M (1991) The occurrence and function of ultradian rhythms. Bioi Rev 66:275–299
Lloyd D, John L, Hamill M, Phillips C, Kader J, Edwards SW (1977) Continuous flow cell cycle fractionation of eukaryotic microorganisms. J Gen Microbiol 99:223–227
Lloyd D, Poole RK, Edwards SW (1982) The cell division cycle: temporal organization and control of cellular growth and reproduction. Academic Press, London
Matin A (1992) Physiology, molecular biology and applications of the bacterial starvation response. J Appl Bacteriol Suppl 73:49S-57S
Mossel DAA, Corry JEL (1977) Detection and enumeration of sublethally injured pathogenic and index bacteria in foods and water processed for safety. Kult und Diff 19–34
Novitsky JA, Morita RY (1976) Morphological characterization of small cells resulting from nutrient starvation of a psychotrophic marine vibrio. Appl Environ Microbiol 32:616–622
Paau AS, Cowles JR, Oro J (1977) Flow-microfluorimetric analysis of Escherichia coli, Rhizobium melilotti, and Rhizobium japonicum at different stages of the growth cycle. Can J Microbiol 23:1165–1169
Paget TA, Lloyd D (1990) Trichomonas vaginalis requires traces of oxygen and high concentrations of carbon dioxide for optimal growth. Mol Biochem Parasitol 41:65–72
Peters DC (1979) A comparison of mercury arc lamp and laser illumination for flow cytometry. J Histochem Cytochem 27:241–245
Phillips AP, Martin KL (1988) Limitations of flow cytometry for the specific detection of bacteria in mixed populations. J Immunol Meth 106: 109–117
Phillips AP, Martin KL, Capey AJ (1987) Direct and indirect immunofluorescence analysis of bacterial populations by flow cytometry. J Immunol Meth 101:219–228
Phillips CA, Lloyd D (1978) Continuous-flow size selection of Tetrahymena pyriformis ST: changes in volume, DNA, RNA and protein during synchronous growth. J Gen Microbiol 105:95–103
Postgate JR, Hunter JR (1962) The survival of starved bacteria. J Gen Microbiol 29:233–263
Postgate JR, Hunter JR (1964) Accelerated death of Aerobacter aerogenes starved in the presence of growth limiting substrates. J Gen Microbiol 34:459–473
Postgate JR, Crumpton JE, Hunter JR (1961) The measurement of bacterial viabilities by slide culture. J Gen Microbiol 24:15–24
Powell EO (1956) A rapid method for determining the proportion of viable bacteria in a culture. J Gen Microbiol 14:153–159
Quesnel (1960) The behaviour of individual organisms in the lag phase and the development of small populations of Escherichia coli J Appl Bacteriol 23:99–105
Rollins DM, Colwell RR (1986) Viable but non-culturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment. Appl Environ Microbiol 52:531–538
Roszak DB, Colwell RR (1987) Metabolic activity of bacterial cells enumerated by direct viable count. Appl Environ Microbiol 53:2889–2983
Roszak DB, Grimes DJ, Colwell RR (1984) Viable but non-recoverable stage of Salmonella enteritidis in aquatic systems. Can J Microbiol 30:334–338
Russell AD (1991) Injured bacteria: occurrence and possible significance. Lett Appl Microbiol 12:1–2
Scott RI, Gibson JF, Poole RK (1980) Adenosine triphosphatase activity and its sensitivity to Ruthenium Red oscillate during the cell cycle of Escherichia coli K12. J Gen Microbiol 120:183–198
Shapiro HM (1983) Multistation multiparameter flow cytometry: a critical review and rationale. Cytometry 3:227–243
Shapiro HM (1985) The little laser that could: applications of low power lasers in clinical flow cytometry. Ann New York Acad Sci 468:18–27
Skarstad K, Boye E (1988) Perturbed chromosomal replication in recA mutants of Escherichia coli J Bacteriol 170:2549–2554
Skarstad K, Steen HB, Boye E (1983) Cell cycle parameters of slowly, growing E. coli B/r studied by flow cytometry. J Bacteriol 154:656–662
Skarstad K, Steen HB, Boye E (1985) DNA distributions of E. coli measured by flow cytometry and compared to theoretical computer simulations. J Bacteriol 163:661–668
Skarstad K, Boye E, Steen HB (1986) Timing of initiation of chromosome replication in individual Escherichia coli cells. EMBO J 5:1711–1717
Skarstad K, von Meyenburg K, Hansen FG, Boye E (1988) Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles. J Bacteriol 170:852–858
Skarstad K, Løbner-OIesen A, Atlung T, von Meyenburg K, Boye E (1989) Initiation of DNA replication in Escherichia coli after overproduction of the DnaA protein. Mol Gen Genet 218:50–56
Slater ML, Sharrow SO, Gart JJ (1977) Cell cycle of Saccharomyces cerevisiae in populations growing at different rates. Proc Natl Acad Sci USA 74:3850–3854
Steen HB, Boye E (1980) Bacterial growth studied by flow cytometry. Cytometry 1:32–36
Steen HB, Boye E (1981) Growth of Escherichia coli studied by dual parameter flow cytometry. J Bacteriol 145:1091–1094
Steen HB, Lindmo T (1979) Flow cytometry: a high resolution instrument for everyone. Science 204:403–404
Steen HB, Boye E, Skarstad K, Bloom B, Godal T, Mustafa S (1982) Applications of flow cytometry on bacteria: cell cycle kinetics, drug effects and quantitation of antibody binding. Cytometry 2:249–257
Steen HB, Lindmo T, Stokke T (1989) Differential light-scattering detection in an arc lampbased flow cytometer. In: Yen A (ed) Flow cytometry: Advanced research and clinical applications, vol. I. CRC Press, Boca Raton, Florida, p 63
Visser G, Reinten C, Coplan P, Gilbert DA, Hammond K (1990) Oscillations in cell morphology and redox state. Biophys Chern 37:383–394
Volkov EI, Stolyarov MN, Brooks RF (1992) The modelling of heterogeneity in proliferative capacity during clonal growth. In: Volkov E (ed) Biophysical approach to complex biological phenomena. Nova, New York, pp 183–203
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Lloyd, D. (1993). Flow Cytometry: A Technique Waiting for Microbiologists. In: Lloyd, D. (eds) Flow Cytometry in Microbiology. Springer, London. https://doi.org/10.1007/978-1-4471-2017-9_1
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DOI: https://doi.org/10.1007/978-1-4471-2017-9_1
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