Abstract
Polyhydroxyalkanoates (PHA) have shown extensive structure variety1. Depending on growth substrates and types of organisms used, the side chain R can change from a simple methyl group to functional structures containing unsaturated double or triple bonds, halogens, benzyl, cyanide or epoxy groups (Fig. 1)2. Both the monomer structure and content affect PHA physical properties. PHA can be very brittle, such as polyhydroxybutyrate (PHB) produced by many bacteria; they can also be flexible, such as PHBV copolyesters consisting of 3-hydroxybutyrate (HB) and 3-hydroxyhexanoate (HHx), and PHBHx copolyesters consisting of HB and 3-hydroxyvalerate (HV). PHA can be elastic too when their monomers are 3-hydroxyoctanoate or 3-hydroxydecanoate (Table 1)3.
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References
Steinbüchel, A., 1991, Polyhydroxyalkanoic acids. In Biomaterials: Novel Materials from Biological Sources (D. Byrom, ed.), Stockton, New York, pp 124–213.
Chen, G.Q., Wu, Q., Zhao, K., and Yu, P.H., 2000, Functional polyhydroxyalkanoates synthesized by microorganisms. Chinese J. Polym. Sci. 5: 389–396.
Doi, Y., Kitamura, S., and Abe, H., 1995, Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 28: 4822–4828.
Hrabak, O., 1992, Industrial production of poly-β-hydroxybutyrate. FEMS Microbiol. Rev. 103:251–256.
Byrom, D., 1992, Production of poly-β-hydroxybutyrate and poly-β-hydroxyvalerate copolymers. FEMS Microbiol. Rev. 103: 247–250.
Chen, G.Q., Zhang, G., Park, S. J., Lee S. Y., 2001, Industrial Production of Poly(hydroxybutyrate-co-hydroxyhexanoate). Appl Microbiol Biotechnol 57: 50–55.
Anderson, A. J., Dawes, E. A., 1990, Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 45: 450–472.
Chen, G. Q., Koenig, K. H., Lafferty, R. M., 1991, Occurrence of poly-D (-)-3-hydroxyalkanoaies in the genus Bacillus. FEMS Microbiol. Lett. 84: 173–176.
Haywood, G. W., Anderson, A. J., Dawes, E. A., 1989, A survey of the accumulation of novel polyhydroxyalkanoates by bacteria. Biotechnol. Lett. 7: 471–476.
Chen, G. Q., Koenig, K. H. Lafferty, R. M., 1991, Production of poly-D(-)-3-hydroxybutyrate and poly-D(-)-3-hydroxyvalerate by strains of Alcaligenes latus. Anton. Leeuw. 60: 61–66.
Hanggi U. J., 1990, Pilot scale production of PHB with Alcaligenes latus. In: Novel Biodegradable Microbial Polymers. (E. D. Dawes ed), Kluwer Academic Publishers, Netherlands, pp. 65–70.
Brandl, H., Cross, R. A., Lenz, R. W., Fuller, C., 1988, Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54: 1977–1982.
Gorenflo, V., Steinbüchel, A., Marose, S., Rieseberg, M., Scheper, T., 1999, Quantification of bacterial polyhydroxyalkanoic acids by Nile red staining, Appl. Microbiol. Biotechnol. 51: 765–772.
Tian, W.D., 1998, Screening and study of novel polyhydroxyalkanoate producing microorganisms. M.Sc. Thesis, Tsinghua University, Beijing/China.
Hong, K., Sun, S.Q., Tian, W.D., Chen, G.Q., 1999, A rapid method for detecting bacterial PHA in intact cells by FT-IR. Appl. Microbiol. Biotechnol. 51: 523–526.
Kansiz, M., Billman-Jacobe, H., McNaughton, D., 2000, Quantitative determination of the biodegradable polymer poly(β-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics. Appl. Environ. Microbiol. 66: 3415–3420.
Wu, Q., Sun, S.Q., Yu, P.H.F., Chan, A.X.Z., Chen, G.Q., 2000, Environmental dependence of microbial synthesis of polyhydroxyalkanoates. Acta Polym. Sin. 6: 751–756.
Macrae, R.M., Wilkinson, J.F., 1958, Poly-beta-hydroxybutyrate metabolism in washed suspensions of bacillus cereus and Bacillus megaterium. J. Gen. Microbiol. 19: 210–220.
Wu, Q., Huang, H.H., Hu, G.H., Chen J.C., Ho, K.P., Chen, G.Q., 2001, Production of poly-3-hydroxybutyrate by Bacillus sp. JMa5 cultivated in molasses media. Anton. Leeuw. 80:111–118.
Küng, W., 1982, Wachstum und Poly-D(-)-3-Hydroxybuttersäure Akkumulation bei Alcaligenes latus. M.Sc. Thesis, Graz University of Technology, Graz/Austria.
Chen, G.Q., 1989, Produktion von Poly-D(-)-3-Hydroxybuttersäure und Poly-D(-)-3-Hydroxyvaleriansäure mit Einzel-und Mischpopulation von Alcaligenes latus DSM 1122, 1123 bzw. 1124. Ph.D. Thesis, Graz University of Technology, Graz/Austria.
Page, W.J., 1992, Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD in beet molasses culture. FEMS Microbiol Rev. 103: 149–158.
Chen, G.Q., Page, W. J., 1994, The effect of substrate on the molecular weight of poly-β-hydroxybutyrate produced by Azotobacter vinelandii UWD. Biotechnol Lett. 16: 155–160.
Chen, G.Q., Page, W. J., 1997, Production of poly-β-hydroxybutyrate by Azotobacter vinelandii UWD in a two-stage fermentation process. Biotechnol. Tech. 11: 347–350.
Hazenberg, W., Witholt, B., 1997, Efficient production of medium-chain-length poly(3-hydroxyalkanoates) from octane by Pseudomonas oleovorans: economic considerations. Appl. Microbiol. Biotech. 48: 588–596.
He, W.N., Tian, W.D., Zhang, G., Chen, G.Q., Zhang, Z.M., 1998, Production of novel polyhydroxyalkanoates by Pseudomonas stutzeri 1317 from glucose and soybean oil. FEMS Microbiol Lett. 169: 45–49.
Zhao, K., Yang, X.S., Chen, J.C., Chen, G.Q., 2002, Effect of lipase treatment on the biocompatibility of microbial polyhydroxyalkanoates. J. Mater. Sci. Mater. Med. 13: 1–6.
Yang, X.S., Zhao, K., Chen, G.Q., 2002, Effect of Surface Treatment on the Biocompatibility of Microbial Polyhydroxyalkanoates. Biomaterials 23: 1391–1397.
Deng, Y., Zhao, K., Zhang X.F., Hu, P., Chen, G.Q., 2002, Study on the three-dimensional proliferation of rabbit articular cartilage-derived chondrocytes on polyhydroxyalkanoate scaffolds. Biomaterials 23: 4049–4056.
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Chen, GQ. (2003). Production and Applications of Microbial Polyhydroxyalkanoates. In: Chiellini, E., Solaro, R. (eds) Biodegradable Polymers and Plastics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9240-6_11
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DOI: https://doi.org/10.1007/978-1-4419-9240-6_11
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