Hansenula polymorpha (Pichia angusta) belongs to a limited number of methylotrophic yeast species. It is able to assimilate nitrate and can grow on a range of carbon sources. Furthermore, H. polymorpha is a thermo-tolerant microorganism with some strains growing at temperatures up to 50° C and more. These unusual characteristics render H. polymorpha attractive as a model organism to study the development and functions of peroxisomes and the biochemistry of nitrate assimilation. H. polymorpha provides an established platform for heterologous gene expression and is distinguished by an impressive track record as producer of recom-binant proteins that include commercially available pharmaceuticals like hepatitis B vaccine, insulin and the IFN α-2a
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
References
Agaphonov, M.O., Beburov, M.Y., Ter-Avanesyan, M.D., and Smirnov, V.N. 1995. Yeast 11:1241–1247.
Agaphonov, M., Trushkina, P. M., Sohn, J.S., Choi, E.S., Rhee, S.K., and Ter-Avanesyan, M.D. 1999. Yeast 15:541–551.
Agaphonov, M.O., Packeiser, A.N., Chechenova, M.B., Choi, E.-S., and Ter-Avanesyan, M.D. 2001. Yeast 18:391–402.
Agaphonov, M.O., Romanos, N.V., Trushina, P. M., Smirnov, V.N., and Ter-Avanesyan, M.D. 2002. BMC Mol. Biol. 3:15–22.
Amuel, C., Gellissen, G., Hollenberg, C.P., and Suckow, M. 2000. Biotechnol. Bioproc. Eng. 5:247–52.
Avgerinos, G.C., Turner, B.G., Gorelick, M.D., Papendieck, A., Weydemann, U., and Gellissen, G. 2001. Sem. Thrombos. Hemostas. 27:357–371.
Avila, J., González, C., Brito, N., and Siverio, J.M. 1998. Biochem. J. 335:647–652.
Avila, J., Pérez, M.D., Brito, N., González, C., and Siverio, J.M. 1995. FEBS Lett. 366:137–142.
Baerends, R.J.S., Sulter, G.J., Jeffries, T.W., Cregg, J.M., and Veenhuis, M. 2002. Yeast 19:37–42.
Barnett, J.A., Payne, R.W., and Yarrow, D. 2000. Yeasts: Characteristics and identification, 3rd edn., Cambridge University Press, Cambridge, UK.
Bartelsen, O., Barnes, C.S., and Gellissen, G. 2002. In: Gellissen G. (Ed.), Hansenula polymorpha — biology and applications, Wiley VCH, Weinheim, pp. 211–228.
Birney, E., and Durbin, R. 2000. Genome Res. 10:547–548.
Bogdanova, A.I., Kustikova, O.S., Agaphonov, M.O., and Ter-Avanesyan, M.D. 1998. Yeast 14:1–9.
Brake, A.J., Merryweather, J.P., Coit, D.G., Heberlein, U.A., Masiarz, F.R., Mullenbach, G.T., Ureda, M.S., Valenzuela, P., and Barr, P.J. 1984. Proc. Natl. Acad. Sci. USA 81:4642–4646.
Bretthauer, R.K., and Castellano, F.J. 1999. Biotechnol. Appl. Biochem. 30:193–200.
Brito, N., Avila, J., Pérez, M.D., González, C., and Siverio, J.M. 1996. Biochem. J. 317:89.–95
Brito, N., Pérez, M.D., Perdomo, G., González, C., García-Lugo, P., and Siverio, J.M. 1999. Appl. Microbiol. Biotechnol. 53:23–29.
Brocke, P., Schaefer, S., Melber, K., Jenzelewski, V., Müller, F., Dahlems, U., Bartelsen, O., Park, K.N., Janowicz, Z.A., and Gellissen, G. 2004. In: Production of recombinant proteins: Novel microbial and eukaryotic expression systems (ed. Gellissen, G.), Wiley VCN, Weinheim, pp. 319–60.
Callewaert, N., Laroy, W., Cadirgi, H., Geysens, S., Saelnes, X., Jou, W.M., and Contreras, R. 2001. FEBS Lett. 503:173–178.
Chiba, Y., Suzuki, M., Yoshida, S., Yoshida, A., Ikenaga, H., Takeuchi, M., Jigami, Y., and Ichishima, E. 1998. J. Biol. Chem. 273:26298–26304.
Cook, M.W., and Thygesen, H.V. 2003. Food Chem. Toxicol. 41:523–529.
Cox, H., Mead, D., Sudbery, P., Eland, M., and Evans, L. 2000. Yeast 16:1191–1203.
Degelmann, A., Müller, F., Sieber, H., Jenzelewski, V., Suckow, M., Strasser, A.W.M., and Gellissen, G. 2002. FEMS Yeast Res. 2:349–361.
Didion, T., and Roggenkamp, R. 1992. FEBS Lett. 303:113–116.
Gellissen, G. 2000. Appl. Microbiol. Biotechnol. 54:741–750.
Gellissen, G., 2002. Hansenula polymorpha — biology and applications. Wiley-VCH, Weinheim.
Gellissen, G. and Hollenberg, C.P. 1997. Gene 190:87–97.
Gellissen, G. and Veenhuis, M. 2001. Yeast 18:1–3.
Gellissen, G., Janowicz, Z.A., Weydemann, U., Melber, K., Strasser, A.W.M., and Hollenberg, C.P. 1992a. Biotechnol. Adv. 10:179–189.
Gellissen, G., Melber, K., Janowicz, S.A., Dahlems, U., Weydemann, U., Piontek, M., Strasser, A. W. M., and Hollenberg, C.P. 1992b. Antonie van Leeuwenhoek 62:79–93.
Gellissen, G., Müller, F., Sieber, H., Tieke, A., Jenzelewski, V., Degelmann, A., and Strasser, A.W.M. 2002. In: Gellissen, G. (Ed.), Hansenula polymorpha — biology and applications, Wiley VCH, Weinheim, pp. 229–254.
Gemmill, T.R. and Trimble, R.B. 1999. Biochim. Biophys. Acta 1426:227–237.
Gleeson, M.A., Ortori, G.S., and Sudbery, P.E. 1986. J. Gen. Microbiol. 132:3459–3465.
Gonzalez, C., Perdomo, G., Tejera, P., Brito, N., and Siverio, J.M. 1999. Yeast 15:1323–1329. Guengerich, L., Kang, H.A., Behle, B., Gellissen, G., and Suckow, M. 2004. (In: Kück U. Ed.), The mycota II. Genetics and Biotechnology Springer-Verlag, Heidelberg, pp. 273–287.
Guengerich, L., Kang, H.A., Behle, B., Gellissen, G., and Suckow, M. 2004. (In: Kück U. Ed.), The mycota II. Genetics and Biotechnology Springer-Verlag, Heidelberg, pp. 273–287.
Hamilton, S.R., Bobrowicz, P., Bobrowicz, B., Davidson, R.C., Li, H., Mitchell, T., Nett, J.H., Rausch, S., Stadheim, T.A., Wischenwski, H., Wildt, S., and Gerngross, T.U. 2003. Science 301:1244 –46.
Hamilton, S.R., Davidson, R.C., Sethuraman, N., Nett, J.H., Jiang, Y., Rios, S., Bobrowicz, P., Stadheim, T.A., Li, H., Choi, B.-K., Hopkins, D., Wischenwski, H., Roser, J., Mitchell, T., Strawbridge, R.R., Hoopes, J., Wildt, S., and Gerngross, T.U. 2006. Science 313:1441–1443.
Hazeu, W., Bruyn, J.C., and de Bos, P. 1972. Arch. Microbiol. 87:185–188.
Hellwig, S., Stöckmann, C., Gellissen, G., and Büchs, G. 2005. In: Production of recombinant proteins: novel microbial and eukaryotic expression systems (Gellissen, G. Ed.), Wiley VCN, Weinheim, pp. 287–317.
Heo, J.H., Hong, W.K., Cho, E.Y., Kim, M.W., Kim, J.Y., Kim, C.H., Rhee, S.K., and Kang, H.A. 2003. FEMS Yeast Res. 4:175–184.
Heo, J.H., Won, H.S., Kang, H.A., Rhee, S.K., and Chung, B.H. 2002. Protein Expr. Purif. 24:117–122.
Hollenberg, C.P. and Janowicz, Z.A. 1988. EPA 0299108.
Janowicz, Z.A., Eckart, M.R., Drewke, C., Roggenkamp, R.O., Hollenberg, C.P., Maat, J., Ledeboer, A.M., Visser, C., and Verrips, C.T. 1985. Nucleic Acids Res. 13:3043–3306.
Jenzelewski, V. 2002. In: Gellissen, G. (Ed.), Hansenula polymorpha — biology and applications, Wiley VCH, Weinheim, pp. 156–174.
Jigami, Y. and Odani, T. 1999. Biochim. Biophys. Acta 1426:335–345.
Kang, H.A. and Gellissen G. 2005. In: Gellissen, G. (Ed.), Production of recombinant proteins: novel microbial and eukaryotic expression systems, Wiley VCN, Weinheim, pp. 111–142.
Kang, H.A., Kim, J.-Y., Ko, S.-M., Park, C.S., Ryu, D.Y., Sohn, J.-H., Choi, E.-S., and Rhee, S.-K. 1998a. Yeast 14:1233–1240.
Kang, H.A., Sohn, J.-H., Choi, E.-S., Chung, B.-H., Yu, M.-H., and Rhee, S.-K. 1998b. Yeast 14:371–381.
Kang, H.A., Kang, W., Hong, W.-K., Kim, M.W., Kim, J.-Y., Sohn, J.-H., Choi, E.-S., Choe, K.-B., and Rhee, S.-K. 2001a. Biotechnol. Bioeng. 76:175–185.
Kang, H.A., Hong, W.-K., Sohn, J.-H., Choi, E.-S., and Rhee, S.K. 2001b. Appl. Microbiol. Biotechnol. 55:734–741.
Kang, H.A., Sohn, J.-H., Agaphonov, M.O., Choi, E.-S., Ter-Avanesyan, M.D., and Rhee, S.K. 2002. Hansenula polymorpha — biology and applications, Wiley VCH, Weinheim, pp. 124–146.
Kim, M.W., Agaphonov, M.O., Kim, J.-Y., Rhee, S.K., and Kang, H.A. 2002. Yeast 19: 863–871.
Kim, M.W., Rhee, S.K., Kim, J.Y., Shimma, Y.I., Chiba, Y., Jigami, Y., and Kang, H.A. 2004. Glyco-biology 14:243–251.
Kim, M.W., Kim, E.J., Kim, J.-Y., Park, J.-S., Oh, D.-B., Shimma, J.-I., Chiba, Y., Jigami, Y., Rhee, S.K., and Kang, H.A. 2006. J. Biol. Chem. 281:6261–6272.
Kim, S.Y., Sohn, S.-H., Kang, H.A., and Choi, E.-S. 2001. Yeast 18:455–461.
Kim, S.Y., Sohn, J.H., Pyun, Y.R., and Choi, E.S. 2002. Yeast 19:1153–1163.
Kim, S.Y., Sohn, J.H., Bae, J.H., Pyun, Y.R., Agaphonov, M.O., and Ter-Avanesyan, M.D., Choi E.S. 2003. Appl. Environ. Microbiol. 69:4448–4454.
Klabunde, J., Diesel, A., Waschk, D., Gellissen, G., Hollenberg, C.P., and Suckow, M. 2002. Appl. Microbiol. Biotechnol. 58:797–805.
Klabunde, J., Kunze, G., Gellissen, G., and Hollenberg, C.P. 2003. FEMS Yeast Res. 4:185–193.
Kurtzman, C.P. 1984. Antonie van Leeuwenhoek 50:209–217.
Kurtzman, C.P. and Fell J.W. 1998. The yeasts, a taxonomic study, Elsevier, Amsterdam, 4th edn.
Kurtzman, C.P. and Robnett C.J. 1998. Antonie van Leeuwenhoek 73:331–371.
Lahtchev, K. 2002. (Gellisen, G. Ed.), Basic genetics of Hansenula Polymorpha. In: biology and applications, Wiley VCH, Weinheim, pp. 8–20.
Ledeboer, A.M., Edens, L., Maat, J., Visser, C., Bos, J.W., Verrips, C.T., Janowicz, Z.A., Eckart, M., Roggenkamp, R.O., and Hollenberg, C.P. 1985. Nucleic Acids Res. 13:3063–3082.
Levine, D.W. and Cooney, C.L. 1973. Appl. Microbiol. 26:982–989.
Mannazzu, I., Gierra, E., Strabbioli, R., Masia, A., Maestrale, G.B., Zoroddu, M.A., and Fatichenti, F. 1997. FEMS Microbiol. Lett. 147:23–28.
Mayer, A.F., Hellmuth, K., Schlieker, H., Lopez-Ulibarri, R., Oertel, S., Dahlems, U., Strasser, A.W.M., van Loon, A.P.G.M. 1999. Biotechnol. Bioeng. 63:373–381.
Middelhoven, W. 2002. (Gellissen, G. Ed.), In: Hansenula polymorpha — biology and applications Wiley VCH, Weinheim, pp. 1–7.
Montesino, R., García, R., Quintero, O., and Cremata, J.A. 1998. Protein Exp. Purif. 14: 197–207.
Morais, J.O.F. and de Maia M.H.D. 1959. Anais de Escola Superior de Quimica de Universidade do Recife 1:15–20.
Müller, S., Sandal, T., Kamp-Hansen, P., and Dalbøge, H. 1998. Yeast 14:1267–1283.
Müller, F. II, Tieke, A., Waschk, D., Mühle, C., Müller, F. I, Seigelchifer, M., Pesce, A., Jenzelewski, V., and Gellissen, G. 2002. Proc. Biochem. 38:15–25.
Ogata, K., Nishikawa, H., and Ohsugi, M. 1969. Agric. Biol. Chem. 33:1519–1520.
Oh, K.S., Kwon, O., Oh, Y.W., Sohn, M.J., Jung, S., Kim, Y.K, Kim, M.G., Rhee, S.K., Gellissen, G., and Kang, H.A. 2004. J. Microbiol. Biotechnol. 14:1239–1248.
Osoegawa, K., de Jong, P.J., Frengen, E., and Ioannou, P.A. 1999. Current Protocols in Human Genetics, 5.15.1–5.15.33.
Osoegawa, K., Woon, P.Y., Zhao, B., Frengen, E., Tateno, M., Catanese, J.J., de Jong, P.J. 1998. Genomics 52:1–8.
Papendieck, A., Dahlems, U., and Gellissen, G. 2002. In: Gelllissen, G. (Ed.), Hansenula poly-morpha — biology and applications, Wiley-VCH, Weinheim, pp. 255–271.
Park, B.S., Ananine, V., Kim, C.H., Rhee, S.K., and Kang, H.A. 2004. Enz. Microb. Technol. 34:132–138.
Park, J.-N., Sohn, M.J., Oh, D.-B., Kwon, O., Rhee, S.K., Hur, C.-G., Lee, S.Y., Gellissen, G., and Kang, H.A. 2007. Appl. Environm. Microbiol. 73:5990–6000.
Parpinello G., Berardi E., and Strabbioli R. 1998. J. Bacteriol. 180:2958–2967.
Perez M.D., Gonzalez C., Avila J., Brito N., Siverio J.M. 1997. Biochem. J. 15:397–403.
Phongdara, A., Merckelbach, A., Keup, P., Gellissen, G., and Hollenberg, C.P. 1998. Appl. Microbiol. Biotechnol. 50:77–84.
Ramezani-Rad, M., Hollenberg, C.P., Lauber, J., Wedler, H., Griess, E., Wagner, C., Albermann, K., Hani, J., Piontek, M., Dahlems, U., and Gellissen, G. 2003. FEMS Yeast Res. 4:207–215.
Reinders, A., Romano, I., Wiemken, A., and de Virgilio, C. 1999. J. Bacteriol. 181:4665–4668.
Roggenkamp, R., Hansen, H., Eckart, M., Janowicz, Z.A., and Hollenberg, C.P. 1986. Mol. Gen. Genet. 202:302–308.
Romano, I. 1998. Ph.D. Thesis, University of Basel, Switzerland.
Schaefer, S., Piontek, M., Ahn, S.-J., Papendieck, A., Janowicz, S.A., and Gellissen, G. 2001 (Dembowsky, K., and Stadler, P. Eds.). In : Novel therapeutic proteins: selected case studies, Wiley-VCN, Weinheim, pp. 245–274.
Schaefer, S., Piontek, M., Ahn, S.-J., Papendieck, A., Janowicz, Z.A., Timmermans, I., Gellissen, G 2002. In: Gellissen, G. (Ed.), Hansenula polymorpha — biology and applications, Wiley-VCH, Weinheim, pp. 175–210.
Siverio, J.M. 2002. In: Gellissen, G. (Ed.), Hansenula polymorpha — biology and applications, Wiley VCH, Weinheim, pp. 21–40.
Sohn, J.-H., Choi, E.-S., Kim, C.-H., Agaphonov, M.O., Ter-Avanesyan, M.D., and Rhee, J.-S., and Rhee, S.-K. 1996. J. Bacteriol. 178:4420–4428.
Sohn, J.-H., Choi, E.-S., Kang, H.A., Rhee, J.-S., and Rhee, S.-K. 1999. J. Bacteriol. 181: 1005–1013.
Steinborn, G., Gellissen, G., and Kunze, G. 2005. FEMS Yeast Res. 5:1047–1054.
Steinborn, G., Böer, E., Scholz, A., Tag K., Kunze G., and Gellissen, G. 2006. Microbial Cell Factories 5:33.
Suckow, M. and Gellissen, G. 2002. In: Gellissen, G. (Ed.), Hansenula polymorpha — biology and applications, Wiley VCH, Weinheim, pp. 105–123.
Sudbery, P. 2003. Yeast 20:1307–1308.
Tani, Y. 1984. In: Hou, C.T. (Ed.), Methylotrophs: microbiology, biochemistry, and genetics, CRC Press, Boca Raton, FL, pp. 55–86.
Teunisson, D.J., Hall, H.H., and Wickerham, L.J. 1960. Mycologia 52:184–188.
Tikhomirova, L.P., Ikonomova, R.N., Kuznetsova, E.N., Fodor, I.I., Bystrykh, L.V., Aminova, L.R., and Trotsenko, Y.A. 1988. J. Basic Microbiol. 28:343–351.
van Dijk, R., Faber, K.N., Hammond, A.T., Glick, B.S., Veenhuis, M., and Kiel, J.A.K.W. 2001. Mol. Genet. Genomics 266:646–656.
van Djik, R., Faber, K.N., Kiel, J.A.K.W., Veenhuis, M., and van der Klei, I.J. 2000. Enzyme Microb. Technol. 26:793–800.
van der Klei, I.J., and Veenhuis, M. 2002. In: Gellissen, G. (Ed.), Hansenula polymorpha — biology and applications Wiley VCH, Weinheim, pp. 76–94.
Veale R.A., Guiseppin M.L., van Eijk H.M., Sudbery P.E., and Verrips C.T. 1992. Yeast 8:361–372.
Weydemann, U., Keup, P., Piontek, M., Strasser, A.W.M., Schweden J., Gellissen G., and Janowicz Z.A. 1995. Appl. Microbiol. Biotechnol. 44:377–385.
Wickerham, L.J. 1951. Technical bulletin No 1029, US Dept Agric, Washington DC, pp. 1–56.
Yurimoto H., Sakai Y., and Kato N. 2002. In: Gellisen, G. (Ed.), Hansenula polymorpha — biology and applications, Wiley VCH, Weinheim, pp. 61–75.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science + Business Media B.V
About this chapter
Cite this chapter
Kunze, G., Kang, H.A., Gellissen, G. (2009). Hansenula polymorpha (Pichia angusta): Biology and Applications. In: Satyanarayana, T., Kunze, G. (eds) Yeast Biotechnology: Diversity and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8292-4_3
Download citation
DOI: https://doi.org/10.1007/978-1-4020-8292-4_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-8291-7
Online ISBN: 978-1-4020-8292-4
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)