In vitro propagation methods, such as somatic embryogenesis (SE), are very interesting approaches when compared with traditional propagation, which presents serious drawbacks. SE is frequently regarded as the best system for propagation of superior genotypes (Kim, 2000) mostly because both root and shoot meristems are present simultaneously in somatic embryos. Cork oak (Quercus suber L.), as other woody species, is recalcitrant concerning somatic embryogenesis (SE). Most of the successful studies regarding SE within this species used juvenile materials (Gallego et al., 1997; Hernandez et al., 1999; Toribio et al., 1999; Hornero et al., 2001a; Pinto et al., 2001). Only recently, SE was successfully and reproducibly induced from adult cork oak trees (Pinto et al., 2002; Lopes et al., 2006), opening perspectives for breeding programmes of selected genotypes of this species.
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Keywords
- Somatic Embryo
- Somatic Embryogenesis
- Somaclonal Variation
- Propidium Iodide Fluorescence
- Internal Reference Standard
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References
Breiman, A., Rotemabarbanell, D., Karp, A. & Shaskin, H. (1987) Heritable somaclonal variation in wild barley (Hordeum spontaneum). Theoretical and Applied Genetics, 74, 104-112.
Bueno, M., Gomez, A., Vicente, O. & Manzanera, J. (1996) Stability in ploidy level during somatic embryogenesis in Quercus canariensis. In M. Ajuha, W. Bourjan & D. Neale (Eds), Somatic Cell Genetics and Molecular Genetics of Trees. Dordrecht: Kluwer Academic Publishers. pp. 23-28.
Clark, J.M. (1988) Novel non-templated nucleotide addition-reactions catalyzed by procaryotic and eukaryotic DNA-polymerases. Nucleic Acids Research 16, 9677-9686.
Conde, P., Loureiro, J. & Santos, C. (2004) Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill. Plant Cell Reports 22, 632-639.
Doležel, J. & Bartoš, J. (2005) Plant DNA flow cytometry and estimation of nuclear genome size. Annals of Botany 95, 99-110.
Doležel, J., Sgorbati, S. & Lucretti, S. (1992) Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiologia Plantarum 85, 625-631.
Doležel, J., Doleželová, M. & Novák, F. (1994) Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata and M. balbisiana). Biologia Plantarum, 36, 351-357.
Doležel, J., Greilhuber, J., Lucretti, S., Meister, A., Lysák, M., Nardi, L., et al. (1998) Plant genome size estimation by flow cytometry: inter-laboratory comparison. Annals of Botany, 82 (Supplement A), 17-26.
Doležel, J., Bartoš, J., Voglmayr, H. & Greilhuber, J. (2003) Nuclear DNA content and genome size of trout and human. Cytometry Part A, 51A, 127-128.
Endemann, M., Hristoforoglu, K., Stauber, T. & Wilhelm, E. (2002) Assessment of age-related polyploidy in Quercus robur L. somatic embryos and regenerated plants using DNA flow cytometry. Biologia Plantarum 44, 339-345.
Galbraith, D.W., Harkins, K.R., Maddox, J.M., Ayres, N.M., Sharma, D.P. & Firoozabady, E. (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science, 220, 1049-1051.
Galbraith, D.W., Lambert, G., Macas, J. & Doležel, J. (2002) Analysis of nuclear DNA content and ploidy in higher plants. In Robinson, J.P., Darzynkiewicz, Z., Dean, P.N., Dressler, L.G., Rabinovitch, P.S., Stewart, C.V., Tanke, H.J. & Wheeless, L.L. (Eds), Current Protocols in Cytometry. New York: John Wiley & Sons, Inc. pp. 7.6.1-7.6.22.
Gallego, F.J., Martinez, I., Celestino, C. & Toribio, M. (1997) Testing somaclonal variation using RAPDs in Quercus suber L. somatic embryos. International Journal of Plant Sciences, 158, 563-567.
Glaubitz, J. & Moran, G. (2000) Genetic tools: the use of biochemical and molecular markers. In Young, A., Boshier, D. & Boyle, T. (Eds), Forest Conservation Genetics: Principles and Practice. Collingwood, Victoria: CSIRO Publishing. pp. 39-59.
Gomez, A., Pintos, B., Aguiriano, E., Manzanera, J.A. & Bueno, M.A. (2001) SSR markers for Quercus suber tree identification and embryo analysis. Journal of Heredity 92, 292-295.
Greilhuber, J., Temsch, E. & Loureiro, J. (2007). Nuclear DNA content measurement. In Doležel, J., Greilhuber, J. & Suda, J. (Eds), Flow Cytometry with Plant Cells. Weinheim: Wiley-VCH. pp. 67-101.
Hernandez, I., Celestino, C., Martinez, J., Hormero, J., Gallego, J. & Toribio, M. (1999) Induction of somatic embryogenesis in leaves from mature Quercus suber trees. Paper presented at the Physiology and control of plant propagation in vitro. Report of activities, Eur Cost Action 822, Krakow, Poland.
Hornero, J., Martinez, I., Celestino, C., Gallego, F. J., Torres, V. & Toribio, M. (2001a) Early checking of genetic stability of cork oak somatic embryos by AFLP analysis. International Journal of Plant Sciences 162, 827-833.
Hornero, J., Gallego, F.J., Martinez, I. & Toribio, M. (2001b) Testing the conservation of Quercus spp. microsatellites in the cork oak, Q. suber L. Silvae Genetica 50(3-4), 162-167.
Isagi, Y. & Suhandono, S. (1997) PCR primers amplifying microsatellite loci of Quercus myrsinifolia Blume and their conservation between oak species. Molecular Ecology 6, 897-899.
Ishii, K., Thakur, R. & Jain, S. (1999) Somatic embryogenesis and evaluation of variability in somatic seedlings of Quercus serrata by RAPD markers. In Jain, S., Gupta, P. & Newton, R. (Eds), Somatic Embryogenesis in Woody Plants. Vol. 4. Dordrecht: Kluwer Academic Publishers. pp. 403-414.
Jones, C.J., Edwards, K.J., Castaglione, S., Winfield, M.O., Sala, F., vandeWiel, C., et al. (1997) Repro- ducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Molecular Breeding 3, 381-390.
Kampfer, S., Lexer, C., Glossl, J. & Steinkellner, H. (1998) Characterization of (GA)(n) microsatellite loci from Quercus robur. Hereditas 129, 183-186.
Karp, A., Edwards, K.J., Bruford, M., Funk, S., Vosman, B., Morgante, M., et al. (1997) Molecular techno- logies for biodiversity evaluation: opportunities and challenges. Nature Biotechnology 15, 625-628.
Kim, Y. (2000) Somatic embryogenesis in Quercus acutissima. In Jain, S., Gupta, P. & Newton, R. (Eds), Somatic Embryogenesis in Woody Plants.Vol. 6. Dordrecht: Kluwer Academic Publishers. pp. 671-686.
Larkin, P.J. & Scowcroft, W.R. (1981) Somaclonal variation - a novel source of variability from cell- cultures for plant improvement. Theoretical and Applied Genetics 60, 197-214.
Larkin, P.J., Banks, P.M., Bhati, R., Brettell, R.I.S., Davies, P.A., Ryan, S.A., et al. (1989) From somatic variation to variant plants - mechanisms and applications. Genome 31, 705-711.
Lopes, T., Pinto, G., Loureiro, J., Costa, A. & Santos, C. (2006) Determination of genetic stability in long-term somatic embryogenic cultures and derived plantlets of cork oak using microsatellite markers. Tree Physiology 26, 1145-1152.
Loureiro, J., Pinto, G., Lopes, T., Doležel, J. & Santos, C. (2005) Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry. Planta 221, 815-822.
Loureiro, J., Rodriguez, E., Doležel, J. & Santos, C. (2006a) Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content. Annals of Botany 98, 515-527.
Loureiro, J., Rodriguez, E., Doležel, J. & Santos, C. (2006b) Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Annals of Botany 98, 679-689.
Loureiro, J., Suda, J., Doležel, J. & Santos, C. (2007a). FLOWer: a plant DNA flow cytometry database. In Doležel, J., Greilhuber, J. & Suda, J. (Eds), Flow Cytometry with Plant Cells. Weinheim: Wiley- VCH. pp. 423-438.
Loureiro, J., Rodriguez, E., Doležel, J. & Santos, C. (2007b) Two new nuclear isolation buffers for plant DNA flow cytometry: A test with 37 species. Annals of Botany (in Press).
Lysák, M. & Doležel, J. (1998) Estimation of nuclear DNA content in Sesleria (Poaceae). Caryologia 52, 123-132.
Noirot, M., Barre, P., Louarn, J., Duperray, C. & Hamon, S. (2000) Nucleus-cytosol interactions - a source of stoichiometric error in flow cytometric estimation of nuclear DNA content in plants. Annals of Botany 86, 309-316.
Pinto, G., Amaral, R., Santos, C. & Carnide, O. (2001) Somatic embryogenesis in calluses of leaves from three year old Quercus suber L. plants. Paper presented at the Quality enhancement of plant production through tissue culture. Book of Abstracts. Second Meeting of the Cost 843 WG3, Carcavelos, Portugal.
Pinto, G., Valentim, H., Costa, A., Castro, S. & Santos, C. (2002) Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree. In Vitro Cellular & Developmental Biology-Plant 38, 569-572.
Pinto, G., Loureiro, J., Lopes, T. & Santos, C. (2004). Analysis of the genetic stability of Eucalyptus globulus Labill. somatic embryos by flow cytometry. Theoretical and Applied Genetics 109, 580-587.
Sanchez, M.C., Martinez, M.T., Valladares, S., Ferro, E. & Vieitez, A.M. (2003) Maturation and germination of oak somatic embryos originated from leaf and stem explants: RAPD markers for genetic analysis of regenerants. Journal of Plant Physiology, 160, 699-707.
Steinkellner, H., Fluch, S., Turetschek, E., Lexer, C., Streiff, R., Kremer, A., et al. (1997a) Identification and characterization of (GA/CT)(n)- microsatellite loci from Quercus petraea. Plant Molecular Biology 33, 1093-1096.
Steinkellner, H., Lexer, C., Turetschek, E. & Glossl, J. (1997b) Conservation of (GA)(n) microsatellite loci between Quercus species. Molecular Ecology 6, 1189-1194.
Thakur, R., Gota, S., Ishii, K. & Jain, S. (1999) Monitoring genetic stability in Quercus serrata Thunb. somatic embryogenesis using RAPD markers. Journal of Forestry Research 4, 157-160.
Tiersch, T.R., Chandler, R.W., Wachtel, S.S. & Elias, S. (1989) Reference standards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry 10, 706-710.
Toribio, M., Celestino, C., Gallego, J. & Martinez, J. (1999) Induction of somatic embryogenesis in tissues from mature oak trees. Paper presented at the Development of integrated systems for large scale propagation of elite plants using in vitro techniques. Report of activities, Eur Cost Action 822, Luxembourg.
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Santos, C., Loureiro, J., Lopes, T., Pinto, G. (2007). Genetic Fidelity Analyses of In Vitro Propagated Cork Oak (Quercus suber L.). In: Jain, S.M., Häggman, H. (eds) Protocols for Micropropagation of Woody Trees and Fruits. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6352-7_7
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