In the survey conducted by the Environmental Agency on endangered species in Japan in the year 2000, 1665 species were listed as endangered one among 7087 vascular plants (Environmental Agency of Japan 2000). Collection for ornamental use, natural succession, and deforestation are the three major causes for threatening the species. To recover the endangered species, propagation of such plants for ex situ or in situ conservation is important. Among them, micropropagation by tissue culture is considered effective and useful method. There were several reports on micropropagation of endangered trees (Okochi et al., 2003; Sugii & Lamoureux, 2004). Here we describe protocols for micropropagation of an endangered five needle pine, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima for preserving it ex situ, and supply plants for rehabilitation.
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Ishii, K., Hosoi, Y., Maruyama, E. (2007). Micropropagation of Pinus armandii var. Amamiana. In: Jain, S.M., Häggman, H. (eds) Protocols for Micropropagation of Woody Trees and Fruits. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6352-7_5
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