The species of the anamorphic genus Botrytis and its associated Botryotinia teleomorphs are briefly assessed. Recent progress in understanding the genetics of variation in the polyphagous B. cinerea (teleomorph Bt. fuckeliana) is summarised, with emphasis on chromosome complement and extrachromosomal elements. Sexual and vegetative compatibility studies are reviewed in relation to the limited evidence of clonality revealed by DNA population markers. It is concluded that in contrast to the traditional view of this species, sexual reproduction plays a major role in determining variation whereas heterokaryosis plays only a limited role. Evidence supporting the existence of a second polyphagous species within B. cinerea sensu lato is discussed. The limited knowledge of the genetics of the hostrestricted species is briefly described.
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Keywords
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- Botrytis Cinerea
- Vegetative Compatibility
- Fungicide Resistance
- Mycological Research
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7. References
Albertini C, Thebaud G, Fournier E and Leroux P (2002) Euburicol 14D-demethylase gene (CVP51) polymorphism and speciation in Botrytis cinerea. Mycological Research 106: 1171-1178
Alfonso C, Raposo R and Melgarejo P (2000) Genetic diversity in Botrytis cinerea populations on vegetable crops in greenhouses in south-eastern Spain. Plant Pathology 49: 243-251
Beever RE and Parkes SL (1993) Mating behaviour and genetics of fungicide resistance of Botrytis cinerea in New Zealand. New Zealand Journal of Crop and Horticultural Science 21: 303-310
Beever RE and Parkes SL (2003) Use of nitrate non-utilising (Nit) mutants to determine vegetative compatibility in Botryotinia fuckeliana (Botrytis cinerea). European Journal of Plant Pathology 109: 607-613
Bergquist RR and Lorbeer JW (1972) Apothecial production, compatibility and sex in Botryotinia squamosa. Mycologia 64: 1270-1281
Bergquist RR and Lorbeer JW (1973) Genetics of variation in Botryotinia squamosa. Mycologia 65: 36-47
Boland GJ (1992) Hypovirulence and double-stranded RNA in Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology 14: 10-17
Brasier CM (1997) Fungal species in practice: identifying species units in fungi. In: Claridge MF, Dawah HA and Wilson MR (eds) Species: The Units of Biodiversity. (pp. 135-170) Chapman & Hall, London, UK
Bridge P, Couteaudier Y and Clarkson J (1998) Molecular Variability of Fungal Pathogens. CAB International, Wallingford, UK
Brygoo Y, Caffier V, Carlier J, Fabre JV, Fernandez D, Giraud T, Mourichon X, Neema C, Notteghem JL, Pope C, Tharreau D and Lebrun MH (1998) Reproduction and population structure in phytopathogenic fungi. In: Bridge P, Couteaudier Y and Clarkson J (eds) Molecular Variability of Fungal Pathogens. (pp. 133-146) CAB International, Wallingford, UK
Buchwald NF (1953) Botryotinia (Sclerotinia) globosa sp. n. on Allium ursinum, the perfect stage of Botrytis globosa Raabe. Phytopathologische Zeitschrift 20: 241-254
Buck KW (1998) Molecular variability of viruses of fungi. In: Bridge P, Couteaudier Y and Clarkson J (eds) Molecular Variability of Fungal Pathogens. (pp. 53-72) CAB International, Wallingford, UK
Büttner P and Tudzynski P (1996) Variation in DNA content and chromosome numbers of Botrytis cinerea. Abstract in: Programme and Book of Abstracts: XI International Botrytis Symposium. Wageningen, The Netherlands, p.13
Büttner P, Koch F, Voigt K, Quidde T, Risch S, Blaich R, Brückner B and Tudzynski P (1994) Variations in ploidy among isolates of Botrytis cinerea: implications for genetic and molecular analyses. Current Genetics 25: 445-450
Castro M, Kramer K, Valdivia L, Ortiz S, Benavente J and Castillo A (1999) A new double-stranded RNA mycovirus from Botrytis cinerea. FEMS Microbiology Letters 175: 95-99
Castro M, Kramer K, Valdivia L, Ortiz S and Castillo A (2003) A double-stranded RNA mycovirus confers hypovirulence-associated traits to Botrytis cinerea. FEMS Microbiology Letters 228: 87-91
Chun SJ and Lee YH (1997) Inheritance of dsRNA in the rice blast fungus Magnaporthe grisea. FEMS Microbiology Letters 148: 159-162
Coenen A, Kevei F and Hoekstra R (1997) Factors affecting the spread of double-stranded RNA viruses in Aspergillus nidulans. Genetical Research (Cambridge) 69: 1-10
Coppin E, Debuchy R, Arnaise S and Picard M (1997) Mating types and sexual development in filamentous Ascomycetes. Microbiology and Molecular Biology Reviews 61: 411-428
Correll JC and Gordon TR (1999) Population structure of Ascomycetes and Deuteromycetes. In: Worrall JJ (ed.) Structure and Dynamics of Fungal Populations. (pp. 225-250) Kluwer Academic Publishers, Dordrecht, The Netherlands
Covert SF (1998) Supernumerary chromosomes in filamentous fungi. Current Genetics 33: 311-319
Daboussi MJ (1996) Fungal transposable elements: generators of diversity and genetic tools. Journal of Genetics 75: 325-339
Dawe AL and Nuss DL (2001) Hypoviruses and chestnut blight: exploiting viruses to understand and modulate fungal pathogenesis. Annual Review of Genetics 35: 1-29
De Miccolis Angelini RM, Santomauro A, De Guido MA, Pollastro S and Faretra F (2002). Genetics of anilinopyrimidine-resistance in Botryotinia fuckeliana (Botrytis cinerea). Abstracts Book of the 6th European Conference on Fungal Genetics, Pisa, Italy, p. 434
De Miccolis Angelini RM, Milicevic T, Natale P, Lepore A, De Guido MA, Pollastro S, Cvjetkovic B and Faretra F (2004) Botryotinia fuckeliana isolates carrying different transposons show differential response to fungicides and localization on host plants. Journal of Plant Pathology (in press)
Debets AJM (1998) Parasexuality in fungi: mechanisms and significance in wild populations. In: Bridge P, Couteaudier Y and Clarkson J (eds) Molecular Variability of Fungal Pathogens. (pp. 41-52) CAB International, Wallingford, UK
Delcán J and Melgarejo P (2002) Mating behaviour and vegetative compatibility in Spanish populations of Botryotinia fuckeliana. European Journal of Plant Pathology 108: 391-400
Deng F, Melzer MS and Boland GJ (2002) Vegetative compatibility and transmission of hypovirulence-associated dsRNA in Sclerotinia homoeocarpa. Canadian Journal of Plant Pathology 24: 481-488
Diolez A, Marches F, Fortini D and Brygoo Y (1995) Boty, a long-terminal-repeat retroelement in the phytopathogenic fungus Botrytis cinerea. Applied and Environmental Microbiology 61: 103-108
Elliott ME (1964) Self-fertility in Botryotinia porri. Canadian Journal of Botany 42: 1393-1395
Faretra F and Antonacci E (1987) Production of apothecia of Botryotinia fuckeliana (de Bary) Whetz. under controlled environmental conditions. Phytopathologia Mediterranea 26: 29-35
Faretra F and Grindle M (1992) Genetic studies of Botryotinia fuckeliana (Botrytis cinerea). In: Verhoeff K, Malathrakis NE and Williamson B (eds) Recent Advances in Botrytis Research. (pp. 7-17) Pudoc Scientific Publishers, Wageningen, The Netherlands
Faretra F and Pollastro S (1991) Genetic basis of resistance to benzimidazole and dicarboximide fungicides in Botryotinia fuckeliana (Botrytis cinerea). Mycological Research 8: 943-951
Faretra F and Pollastro S (1993) Genetics of sexual compatibility and resistance to benzimidazole and dicarboximide fungicides in isolates of Botryotinia fuckeliana (Botrytis cinerea) from nine countries. Plant Pathology 42: 48-57
Faretra F and Pollastro S (1996) Genetic studies of the phytopathogenic fungus Botryotinia fuckeliana (Botrytis cinerea) by analysis of ordered tetrads. Mycological Research 100: 620-624
Faretra F, Antonacci E and Pollastro S (1988) Sexual behaviour and mating system of Botryotinia fuckeliana, teleomorph of Botrytis cinerea. Journal of General Microbiology 134: 2543-2550
Faretra F, Pollastro S, Santomauro A and Miazzi M (1996). Genetics of Botryotinia fuckeliana (Botrytis cinerea): an overview. In: Programme and Book of Abstracts of the XI International Botrytis Symposium. Wageningen, The Netherlands, p. 11
Farr DF, Bills GF, Chamuris GP and Rossman AY (1989) Fungi on Plants and Plant Products in the United States. American Phytopathological Society Press, St. Paul, Minnesota, USA
Ford EJ, Miller RV and Sherwood JE (1995) Heterokaryon formation and vegetative compatibility in Sclerotinia sclerotiorum. Mycological Research 99: 241-247
Fournier E, Giraud T, Loiseau A, Vautrin D, Estoup A, Solignac M, Cornuet JM and Brygoo Y (2002) Characterization of nine polymorphic microsatellite loci in the fungus Botrytis cinerea (Ascomycota). Molecular Ecology Notes 2: 253-255
Fournier E, Levis C, Fortini D, Leroux P, Giraud T and Brygoo Y (2003) Characterization of Bc-hch, the Botrytis cinerea homolog of the Neurospora crassa het-c vegetative incompatibility locus and its use as a population marker. Mycologia 95: 251-261
Free SJ, Holtz BA and Michailides TJ (1996) Mating behavior in field populations of Monilinia fructicola. Mycologia 88: 208-211
Ghabrial SA (1994) New developments in fungal virology. Advances in Virus Research 43: 303-388
Giraud T, Fortini D, Levis C, Leroux P and Brygoo Y (1997) RFLP markers show genetic recombination in Botryotinia fuckeliana (Botrytis cinerea) and transposable elements reveal two sympatric species. Molecular and Biological Evolution 14: 1177-1185
Giraud T, Fortini D, Levis C, Lamarque C, Leroux P, LoBuglio K and Brygoo Y (1999) Two sibling species of the Botrytis cinerea complex, transposa and vacuma, are found in sympatry on numerous host plants. Phytopathology 89: 967-973
Glass NL, Jacobson DJ and Shiu PKT (2000) The genetics of hyphal fusion and vegetative incompatibility in filamentous ascomycete fungi. Annual Review of Genetics 34: 165-186
Godfrey GH (1923) Gray mold of castor bean. Journal of Agricultural Research 23: 679-716
Gregory PH (1949) Studies on Sclerotinia and Botrytis II. De Bary's description and specimens of Peziza fuckeliana. Transactions British Mycological Society 30: 1-13
Griffiths AJF (1995) Natural plasmids of filamentous fungi. Microbiological Reviews 59: 673-685
Griffiths AJF, Collins, RA and Nargang FE (1995) Mitochondrial genetics of Neurospora. In: Kück U (ed.) The Mycota II Genetics and Biotechnology. Springer-Verlag, Berlin,
Germany Grindle M (1979) Phenotypic differences between natural and induced variants of Botrytis cinerea. Journal of General Microbiology 111: 109-120
Hansen HN (1938) The dual phenomenon in imperfect fungi. Mycologia 30: 442-455
Harrington TC and Rizzo DM (1999) Defining species in the fungi. In: Worrall JJ (ed.) Structure and Dynamics of Fungal Populations. (pp.43-121) Kluwer Academic Publishers, Dordrecht, The Netherlands
Harrison JC (1988) The biology of Botrytis spp. on Vicia beans and chocolate spot disease - a review. Plant Pathology 37: 168-201
Hennebert GL (1963) Les Botrytis des Allium. Mededelingen Van de Landbouwhogeschool En de Opzoekingsstations Van de Staat Te Gent 28: 851-876
Hennebert GL (1973) Botrytis and Botrytis-like genera. Persoonia 7: 183-204
Hennebert GL and Groves JW (1963) Three new species of Botryotinia on Ranunculaceae. Canadian Journal of Botany 41: 341-373
Hiratsuka K, Namba S, Yamashita S and Doi Y (1987) Linear plasmid-like DNA's in the fungus Botrytis cinerea. Annals of the Phytopathological Society of Japan 53: 638-642
Holst-Jensen A and Schumacher T (1994) Sclerotiniaceous species on Rubus chamaemorus: morphoanatomical and RFLP studies. Mycological Research 98: 923-930
Holst-Jensen A, Vaage M and Schumacher T (1998) An approximation to the phylogeny of Sclerotinia and related genera. Nordic Journal of Botany 18: 705-719
Holst-Jensen A, Vaage M, Schumacher T and Johansen S (1999) Structural characteristics and possible horizontal transfer of group I introns between closely related plant pathogenic fungi. Molecular Biological Evolution 16: 114-126
Howitt, RLJ (1998) Characterisation of mycoviruses in the plant pathogenic fungus, Botrytis cinerea. PhD thesis, University of Auckland, New Zealand.
Howitt RLJ, Beever RE, Pearson MN and Forster RLS (1995) Presence of double-stranded RNA and virus-like particles in Botrytis cinerea. Mycological Research 99: 1472-1478
Howitt RL, Beever RE, Pearson MN and Forster RL (2001) Genome characterization of Botrytis virus F, a flexuous rod-shaped mycovirus resembling plant 'potex-like' viruses. Journal of General Virology 82: 67-78
Huang J, Hsieh TF, Chastagner GA and Hsiang T (2001) Clonal and sexual propagation in Botrytis elliptica. Mycological Research 105: 833-842
Hutson R and Mansfield J (1980) A genetical approach to the analysis of mechanisms of pathogenicity in Botrytis/Vicia faba interactions. Physiological Plant Pathology 17: 309-317
Jarvis WR (1977) Botryotinia and Botrytis Species: Taxonomy, Physiology, and Pathogenicity. Research Branch, Canada Department of Agriculture, Ottawa, Canada
Jarvis WR (1980) Taxonomy. In: Coley-Smith JR, Verhoeff K and Jarvis WR (eds) The Biology of Botrytis. (pp. 1-18) Academic Press, London, UK
Kerssies A, Bosker-Van Zessen AI, Wagemakers CAM and Van Kan JAL (1997) Variation in pathogenicity and DNA polymorphism among Botrytis cinerea isolates sampled inside and outside a glasshouse. Plant Disease 81: 781-786
Kidwell MG and Lisch DR (2001) Perspective: transposable elements, parasitic DNA, and genome evolution. Evolution 55: 1-24
Kohn LM (1979a) A monographic revision of the genus Sclerotinia. Mycotaxon 9: 365-444
Summers RW, Heaney SP and Grindle M (1984) Studies of a dicarboximide resistant heterokaryon of Botrytis cinerea. British Crop Protection Conference: Pests and Disease 2: 453-458
Sun D (1989) Heterokaryosis in Botrytis squamosa. Acta Mycologica Sinica 8: 311-315
Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS and Fisher MC (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genetics and Biology 31: 21-32
Thompson JR and Latorre BA (1999) Characterization of Botrytis cinerea from table grapes in Chile using RAPD-PCR. Plant Disease 83: 1090-1094
Tolmsoff WJ (1983) Heteroploidy as a mechanism of variability among fungi. Annual Review of Phytopathology 21: 317-340
Typas M, Mavridou A and Kramer K (1998) Mitochondrial DNA differences provide maximum intraspecific polymorphism in the entomopathogenic fungi Verticillium lecanii and Metarhizium anisopliae, and allow isolate detection/identification. In: Bridge P, Couteaudier Y and Clarkson J (eds) Molecular Variability of Fungal Pathogens. (pp. 227-238) CAB International, Wallingford, UK
Uhm JY and Fuji H (1983) Heterothallism and mating type mutation in Sclerotinia trifoliorum. Phytopathology 73: 569-572
Vallejo I, Carbú M, Muñoz F, Rebordinos L and Cantoral J.M. (2002) Inheritance of chromosome-length polymorphisms in the phytopathogenic ascomycete Botryotinia fuckeliana (anam. Botrytis cinerea). Mycological Research 106: 1075-1085
Vallejo I, Santos M, Cantoral JM, Collado IG and Rebordinos L (1996) Chromosomal polymorphism in Botrytis cinerea strains. Hereditas 124: 31-38
Van den Ende E and Pennock I (1996) The perfect stage of Botrytis elliptica. In: Book of Abstracts of the XI International Botrytis Symposium. Wageningen, The Netherlands, p.16
Van den Ende JE and Pennock-Vos IMG (1997) Primary sources of inoculum of Botrytis elliptica in lily. Acta Horticulturae No. 430: 591-595
Van der Vlugt-Bergmans CJB, Brandwagt BF, Van't Klooster JW, Wagemakers CAM and Van Kan JAL (1993) Genetic variation and segregation of DNA polymorphisms in Botrytis cinerea. Mycological Research 97: 1193-1200
Van Kan JAL, Goverse A and Van der Vlugt-Bergmans CJB (1993) Electrophoretic karyotype analysis of Botrytis cinerea. Netherlands Journal of Plant Pathology 99: 119-128
Vilches S and Castillo A (1997) A double-stranded RNA mycovirus in Botrytis cinerea. FEMS Microbiological Letters 155: 125-130
Weeds PL, Beever RE and Long PG (1998) New genetic markers for Botrytis cinerea (Botryotinia fuckeliana). Mycological Research 102: 791-800
Wu TH and Lu JY (1991) A new species of Botryotinia - the teleomorph of Botrytis fabae Sardiña. Acta Mycologia Sinica 10: 27-30
Yamamoto W (1959) Species of the Sclerotiniaceae in Japan. Transactions of the Mycological Society Japan 2: 2-8
Yohalem DS, Nielsen K and Nicolaisen M (2003) Taxonomic and nomenclatural clarification of the onion neck rotting Botrytis species. Mycotaxon 85: 175-182
Yourman LF, Jeffers SN and Dean RA (2000) Genetic analysis of isolates of Botrytis cinerea sensitive and resistant to benzimidazole and dicarboximide fungicides. Phytopathology 90: 851-859
Yunis H and Elad Y (1989) Survival of dicarboximide-resistant strains of Botrytis cinerea in plant debris during summer in Israel. Phytoparasitica 17: 13-21
Zolan ME (1995) Chromosome-length polymorphism in fungi. Microbiological Reviews 59: 686-698
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Beever, R.E., Weeds, P.L. (2007). Taxonomy and Genetic Variation of Botrytis and Botryotinia. In: Elad, Y., Williamson, B., Tudzynski, P., Delen, N. (eds) Botrytis: Biology, Pathology and Control. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2626-3_3
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