Abstract
The detection of gene expression can be accomplished in several ways. “Classical” methods (still important, of course) relied on the observation of biochemical or phenotypic changes in cells or organisms in order to determine the expression of a specific gene. Later, advances in macromolecular separation technology made feasible the identification and isolation of a particular gene product or protein molecule. With the advent of recombinant DNA techniques, it is now possible to detect and analyze the transcriptional product of any gene. There are several methods now in widespread use for studying specific RNA molecules. These methods include in situ hybridization,1 Northern gels,2 dot-or slot-blots,2,3 S-1 nuclease assays,4 and RNase protection studies.5 In this chapter, we describe a new and powerful method to detect gene expression at the RNA level. Protocols will be outlined and discussed in detail and some applications will be briefly described. No attempt has been made to exhaustively review the literature in this fast moving area, so we wish to apologize beforehand to the numerous authors whose work may have not been cited in this chapter.
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Kawasaki, E.S., Wang, A.M. (1989). Detection of Gene Expression. In: Erlich, H.A. (eds) PCR Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-20235-5_8
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DOI: https://doi.org/10.1007/978-1-349-20235-5_8
Publisher Name: Palgrave Macmillan, London
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