Abstract
The PCR method has provided a substitute for most of the repetitive types of molecular cloning and template preparation for sequencing. In combination with automated sequencing techniques, PCR will provide the fastest and most efficient means of generating nucleotide acid sequence information. The purpose of this chapter is to review methods for preparation of sequencing templates and performing direct sequencing of PCR products.
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© 1989 Stockton Press
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Gyllensten, U. (1989). Direct Sequencing of In Vitro Amplified DNA. In: Erlich, H.A. (eds) PCR Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-20235-5_5
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DOI: https://doi.org/10.1007/978-1-349-20235-5_5
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-0-333-48948-2
Online ISBN: 978-1-349-20235-5
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