Abstract
Sample preparation for PCR can be as simple and rapid as adding cells directly to the PCR.1 Things are not quite this easy, however, for all samples and applications of PCR. In this chapter, I will discuss what determines the amount of preparation necessary and present example protocols for the rapid preparation for PCR of DNA and RNA from various sources.
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Saiki, R.K, Bugawan, T.L., Horn, G.T., Mullis, K.B., and Erlich, H.A. (1986) Nature 324:163.
Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K., and Erlich, H.A. (1988) Science 239:487.
Tsukumoto, A.S., Grosschedl, R., Guzman, R.C., Parslow, T., and Varmus, H.E. (1988) Cell 55:619.
Lawyer, F.C., Stoffel, S., Saiki, R.K., Myambo, K., Drummond, R., and Gelfand, D.H. J. Biol. Chem in press.
Ou, C., Kwok, S., Mitchell, S.W., Mack, D.H., Sninsky, J.J., Krebs, J.W., Feorino, P., Warfield, D., and Schochetman, G. (1988) Science 239:295.
Kogan, S.C., Doherty, M., and Gitschier, J. (1987) N. Engl. J. Med. 317:985.
Higuchi, R. (1989) Perkin Elmer/Cetus Newsletter Amplifications 2:1.
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© 1989 Stockton Press
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Higuchi, R. (1989). Simple and Rapid Preparation of Samples for PCR. In: Erlich, H.A. (eds) PCR Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-20235-5_4
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DOI: https://doi.org/10.1007/978-1-349-20235-5_4
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-0-333-48948-2
Online ISBN: 978-1-349-20235-5
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