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A New Approach to Constructing Genetic Maps: PCR Analysis of DNA Sequences in Individual Gametes

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PCR Technology

Abstract

The construction of genetic maps in higher organisms depends upon the ability to analyze the progeny of selected matings or to compute linkage relationships by means of pedigree analysis. In humans, only the latter is possible. Using restriction fragment length polymorphisms (RFLPs), there has been significant progress towards the construction of a human linkage map (for reviews see 1–3). To locate genes with known phenotypic effects relative to RFLP markers, there has been a concerted effort to establish a panel of genetic markers at about 10-cM intervals (on the average, 1 cM = 1% recombination) so that no gene will be further than 5 cM away from an RFLP marker.4 Pedigree analysis is thought to be able to measure genetic distances to a resolution of approximately 1 cM (encompassing about 1000 kb of DNA) with statistical reliability.5 The analysis of smaller genetic distances requires an examination of such a large number of individuals from informative families that it is impractical.

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Authors
  1. Norman Arnheim
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Editor information

Editors and Affiliations

  1. Department of Human Genetics, Cetus Corporation, USA

    Henry A. Erlich Ph.D. (Senior Scientist, Director) (Senior Scientist, Director)

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© 1989 Stockton Press

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Arnheim, N. (1989). A New Approach to Constructing Genetic Maps: PCR Analysis of DNA Sequences in Individual Gametes. In: Erlich, H.A. (eds) PCR Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-20235-5_12

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