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Analysis of Chitin and Chitosan

  • Chapter
Chitin Chemistry

Abstract

When considering the analysis of chitin and chitosan it must be remembered that there is no definitive ‘standard’ material for either polymer. Thus in many instances analysis is carried out in order to characterise the material rather than to determine whether or not it conforms to a definite structure. In the case of commercial products, much of the analysis is again a matter of characterisation since in many applications it is not critical whether, for example, the sample is chitosan[0.20] or chitosan[0.10]. However in a number of important applications the intended end-use will impose certain critical specifications such as the absence of toxic heavy metals or of residual protein, which might be allergenic, in chitin and chitosan intended for medical applications. Proposed standards for chitosan for use in pharmaceutical and medical applications have been published.1 These cover general characteristics, chemical and microbiological purity levels, physiological properties and biological activity.

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References

  1. J. Knapczyk, L. Krówczynski, J. Krzek, M. Brzeski, E. Nürnberg, D. Schenk and H. Struszczyk, in Chitin and Chitosan, G. Skjåk-Braek, T. Anthonsen and P. Sandford (eds), Elsevier, London, 1989, p. 657.

    Google Scholar 

  2. K. Kurita, T. Sannan and Y. Iwakura, Makromol. Chem., 178 (1977) 3197.

    Article  Google Scholar 

  3. S. Hirano, S. Tsuneyasu and Y. Kondo, Agric. Biol. Chem., 45 (1981) 1335.

    Article  Google Scholar 

  4. H. Braconnot, Ann. Chim. (Paris), 79 (1811) 265.

    Google Scholar 

  5. G. Ledderhose, Ber., 9 (1876) 1200.

    Article  Google Scholar 

  6. H. Brach and O. von Fürth, Biochem. Zeit., 38 (1912) 468.

    Google Scholar 

  7. B. Radhakrishnamurthy, E.R. Dalferes and G.S. Berenson, Anal. Biochem., 26 (1978) 61.

    Article  Google Scholar 

  8. Z. Holan, J. Votruba and V. Vlasáková, J. Chromatog., 190 (1980) 67.

    Article  Google Scholar 

  9. A. Elek and R.A. Harte, Ind. Eng. Chem., Anal. Ed., 8 (1936) 267.

    Article  Google Scholar 

  10. T. Sannan, K. Kurita and Y. Iwakura, Makromol. Chem., 177 (1976) 3589.

    Article  Google Scholar 

  11. T. Sannan, K. Kurita, K. Ogura and Y. Iwakura, Polymer, 19 (1978) 458.

    Article  Google Scholar 

  12. C.K. Kandaswamy, in Proceedings 1st International Conference on Chitin/Chitosan (1977), R.A.A. Muzzarelli and E.R. Pariser (eds), MIT Sea Grant Report MITSG 78-7, 1978, p. 517.

    Google Scholar 

  13. N. Gowri, G. Sundara Rajulu and M. Aruchami, in Chitin and Chitosan, S. Hirano and S. Tokura (eds), The Japanese Society of Chitin and Chitosan, Tottori, 1982, p. 77.

    Google Scholar 

  14. F.A. Rutherford and P.R. Austin, in ref. 12, p. 182.

    Google Scholar 

  15. L.J. Filar and M.G. Wirick, in ref. 12, p. 169.

    Google Scholar 

  16. R.U. Lemieux and C.B. Purves, Can. J. Res., 25B (1947) 485.

    Article  Google Scholar 

  17. S.E. Darmon and K.M. Rudall, Disc. Faraday Soc, 9 (1950) 251.

    Article  Google Scholar 

  18. G.K. Moore and G.A.F. Roberts, in ref. 12, p. 421.

    Google Scholar 

  19. G.K. Moore and G.A.F. Roberts, Int. J. Biol. Macromol., 2 (1980) 115.

    Article  Google Scholar 

  20. J. G. Domszy and G.A.F. Roberts, Makromol. Chem., 186 (1985) 1671.

    Article  Google Scholar 

  21. M. Miya, R. Iwamoto, K. Ogura and Y. Iwakura, Int. J. Biol. Macromol., 2 (1980) 323.

    Article  Google Scholar 

  22. S. Aiba, Int. J. Biol. Macromol., 8 (1986) 173.

    Article  Google Scholar 

  23. M. Miya, R. Iwamoto, K. Ohta and S. Mima, Kobunshi Ronbunshu, 42 (1985) 181.

    Article  Google Scholar 

  24. A. Domard and M. Rinaudo, Int. J. Biol. Macromol., 5 (1983) 49.

    Article  Google Scholar 

  25. B.D. Gummow and G.A.F. Roberts, Makromol. Chem., 186 (1985) 1239.

    Article  Google Scholar 

  26. G.G. Maghami and G.A.F. Roberts, Makromol. Chem., 189 (1988) 2239.

    Article  Google Scholar 

  27. R.A.A. Muzzarelli, F. Tanfani, G. Scarpini and G. Laterza, J. Biochem. Biophys. Methods, 2 (1980) 299.

    Article  Google Scholar 

  28. P. Broussignac, Chem. Ind. Genie Chim., 99 (1968) 1241.

    Google Scholar 

  29. H. Terayama, J. Pol. Sci., 8 (1952) 243.

    Article  Google Scholar 

  30. A.D. Domard, Int. J. Biol. Macromol., 9 (1987) 333.

    Article  Google Scholar 

  31. A. Baxter, M. Dillon, K.D.A. Taylor and G.A.F. Roberts, Int. J. Biol. Chem., submitted for publication.

    Google Scholar 

  32. J.E. Castle, J.R. Deschamps and K. Tice, in Chitin, Chitosan and Related Enzymes, J.P. Zikakis (ed.), Academic Press, London, 1984, p. 273.

    Google Scholar 

  33. R.A.A. Muzzarelli and R. Rocchetti, in Chitin in Nature and Technology, R.A.A. Muzzarelli, C. Jeuniaux and G.W. Gooday (eds), Plenum Press, New York, 1986, p. 385.

    Chapter  Google Scholar 

  34. A. Stone, Biopolymers, 7 (1969) 173.

    Article  Google Scholar 

  35. E. Kabat, K. Lloyd and S. Beychock, Biochemistry, 8 (1969) 747.

    Article  Google Scholar 

  36. A. Domard, Int. J. Biol. Macromol., 8 (1986) 243.

    Article  Google Scholar 

  37. L.A. Buffington and E.S. Stevens, J. Amer. Chem. Soc., 101 (1979) 5159.

    Article  Google Scholar 

  38. A. Domard, Int. J. Biol. Macromol., 9 (1987) 98.

    Article  Google Scholar 

  39. S. Hirano and R. Yamaguchi, Biopolymers, 15 (1976) 1685.

    Article  Google Scholar 

  40. R.C. Capozza, German Patent 2,505,305 (1975).

    Google Scholar 

  41. H. Saito, R. Tabeta and S. Hirano, in ref. 13, p. 71.

    Google Scholar 

  42. G.K. Moore, Ph.D. Thesis (CNAA), Trent Polytechnic, UK, 1978.

    Google Scholar 

  43. F.G. Donnan, Chem. Rev., 1 (1924) 73.

    Article  Google Scholar 

  44. E.R. Hayes and D.H. Davies, in ref. 12, p. 406.

    Google Scholar 

  45. G.G. Maghami and G.A.F. Roberts, Makromol. Chem., 189 (1988) 195.

    Article  Google Scholar 

  46. R. Jeanloz and E. Forchielli, Helv. Chim. Acta, 33 (1950) 1690.

    Article  Google Scholar 

  47. G.K. Moore and G.A.F. Roberts, Int. J. Biol. Macromol., 3 (1981) 337.

    Article  Google Scholar 

  48. W.A. Neugebauer, E. Neugebauer and R. Brzezinski, Carbohyd. Res., 189 (1989) 363.

    Article  Google Scholar 

  49. J.G. Domszy, Ph.D. Thesis (CNAA), Trent Polytechnic, UK, 1983.

    Google Scholar 

  50. Y. Araki and E. Ito, Biochem. Biophys. Res. Commun., 56 (1974) 669.

    Article  Google Scholar 

  51. Y. Araki and E. Ito, Eur. J. Biochem., 55 (1975) 71.

    Article  Google Scholar 

  52. J.G. Domszy and G.A.F. Roberts, Int. J. Biol. Macromol., 7 (1985) 45.

    Article  Google Scholar 

  53. P.R. Austin, US Patent 4,063,016 (1977).

    Google Scholar 

  54. J. Mattai and E.R. Hayes, J. Anal. Appl. Pyrolysis, 3 (1982) 327.

    Article  Google Scholar 

  55. G.S. Lal and E.R. Hayes, J. Anal. Appl. Pyrolysis, 6 (1984) 183.

    Article  Google Scholar 

  56. D.H. Davies, E.R. Hayes and G.S. Lal, in ref. 33, p. 365.

    Google Scholar 

  57. D.H. Davies and E.R. Hayes, in Methods in Enzymology, W.A. Wood and S.T. Kellogg (eds), Academic Press, London, 1988, vol. 161, p. 442.

    Google Scholar 

  58. G. Alonso, C. Peniche-Covas and J.M. Nieto, J. Therm. Anal., 28 (1983) 189.

    Article  Google Scholar 

  59. A. Domard, and M. Rinaudo, in ref. 3, p. 315.

    Google Scholar 

  60. M. Terbojevich, C. Carraro, A. Cosani and E. Marsano, Carbohyd. Res., 180 (1988) 73.

    Article  Google Scholar 

  61. M. Rinaudo and A. Domard, in ref. 1, p. 71.

    Google Scholar 

  62. G.A.F. Roberts and J.G. Domszy, Int. J. Biol. Macromol., 4 (1982) 374.

    Article  Google Scholar 

  63. K. Nagasawa, Y. Tohira, Y. Inoue and N. Tanoura, Carbohydr. Res., 18 (1971) 95.

    Article  Google Scholar 

  64. K. Nagasawa and N. Tanoura, Chem. Pharm. Bull., 20 (1972) 157.

    Article  Google Scholar 

  65. V.F. Lee, University Microfilms (Ann Arbor) 74/29446, 1974.

    Google Scholar 

  66. L.A. Berkovich, G.I. Timofeyeva, M.G. Tsyurupa and V.A. Davankov, Vysokomol. Soedin., Ser. A, 22 (1980) 1834.

    Google Scholar 

  67. N.V. Pogodina, G.M. Pavlov, S.V. Bushin, A.B. Mel’nikov, Ye. B. Lysenko, L.A. Nud’ga, V.N. Marcheva, G.N. Marchenko and V.N. Tsvetkov, Polym. Sci. U.S.S.R., 28 (1986) 251.

    Article  Google Scholar 

  68. K.H. Meyer, R.P. Piroué and M.E. Odier, Helv. Chim. Acta, 35 (1952) 574.

    Article  Google Scholar 

  69. P.J. Van Duin and J.J. Hermans, J. Pol. Sci., 36 (1959) 295.

    Article  Google Scholar 

  70. R.A.A. Muzzarelli, A. Ferrero and M. Pizzoli, Talanta, 19 (1972) 1222.

    Article  Google Scholar 

  71. M. Terbojevich, A. Cosani, M. Scandola and A. Fornasa, in ref. 33, p. 349.

    Google Scholar 

  72. A. Domard and M. Rinaudo, Polym. Comm., 25 (1984) 55.

    Google Scholar 

  73. R.A.A. Muzzarelli, C. Lough and M. Emanuelli, Carbohyd. Res., 164 (1987) 433.

    Article  Google Scholar 

  74. R.H. Hackman and M. Goldberg, Carbohyd. Res., 38 (1974) 35.

    Article  Google Scholar 

  75. A.C.M. Wu, W.A. Bough, E.C. Conrad and K.E. Alden, J. Chromatog., 128 (1976) 87.

    Article  Google Scholar 

  76. A.C.M. Wu and W.A. Bough, in ref. 12, p. 88.

    Google Scholar 

  77. S. Mima, M. Miya, R. Iwamoto and S. Yoshikawa, in ref. 13, p. 21.

    Google Scholar 

  78. A.C.M. Wu, in ref. 57, p. 447.

    Google Scholar 

  79. P.J. Flory, Principles of Polymer Chemistry, Cornell University Press, Ithaca, 1953, p. 321.

    Google Scholar 

  80. A.I. Gamzazade, V.M. Shlimak, A.M. Sklyar, E.V. Shtykova, S.A. Pavlova and S.V. Rogozhin, Acta Polym., 36 (1985) 420.

    Article  Google Scholar 

  81. A. Charlesby, J. Pol. Sci., 15 (1955) 263.

    Article  Google Scholar 

  82. A Sharpies and H.M. Major, J. Pol. Sci., 27 (1958) 433.

    Article  Google Scholar 

  83. G. Sitaramaih and D.A.I. Goring, J. Pol. Sci., 58 (1962) 1107.

    Article  Google Scholar 

  84. K. Kamida and K. Okajima, Pol. J., 13 (1981) 163.

    Article  Google Scholar 

  85. Y. Takiguchi, K. Ohkouchi, O. Okada and K. Shimahara, Seikai Daigaku Kogaku Hokoku 1 (1984) 2451.

    Google Scholar 

  86. K. Shimahara and Y. Takiguchi, in ref. 57, p. 417.

    Google Scholar 

  87. K. Shimahara, K. Ohkouchi and M. Ikeda, in ref. 13, p. 10.

    Google Scholar 

  88. O.L. Oke, S.O. Talabi, and I.B. Umoch, in ref. 12, p. 327.

    Google Scholar 

  89. C.J. Brine, in ref. 13, p. 105.

    Google Scholar 

  90. G.A.F. Roberts and F.A. Wood, Report to the Highlands and Islands Development Board, 1983.

    Google Scholar 

  91. American Society for Testing Materials, ASTM D1925.

    Google Scholar 

  92. R.A.A. Muzzarelli, in The Polysaccharides, G.O. Aspinall (ed.), Academic Press, New York, 1985, vol. 3, p. 417.

    Google Scholar 

  93. Official Methods of Analysis of the Association of Official Analytical Chemists, W. Horwitz (ed.), AOAC, Washington, 13th edn, 1980.

    Google Scholar 

  94. British Standard 4407, 1989.

    Google Scholar 

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© 1992 George A.F. Roberts

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Roberts, G.A.F. (1992). Analysis of Chitin and Chitosan. In: Chitin Chemistry. Palgrave, London. https://doi.org/10.1007/978-1-349-11545-7_3

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  • DOI: https://doi.org/10.1007/978-1-349-11545-7_3

  • Publisher Name: Palgrave, London

  • Print ISBN: 978-1-349-11547-1

  • Online ISBN: 978-1-349-11545-7

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