Abstract
Laser assisted confocal microscopy has made a lot of progress over the past few years. Laser systems have become more modular and compact. There is an ever-increasing number of available laser excitation lines as well as an improvement in user friendliness and ease of use. At the same time, expansion of Web resources has provided easy access to a wealth of information. Our goal is both to aid the experienced and novice microscopist in quickly locating and sorting through the relevant laser information and to provide a means of avoiding common problems and pitfalls in the use of laser excitation in the various fluorescence techniques such as fluorescence correlation spectroscopy (FCS), fluorescence lifetime imaging microscopy (FLIM), fluorescence loss in photobleaching (FLIP), fluorescence recovery after photobleaching (FRAP), optical coherence tomography (OCT), second harmonic generation (SHG), single molecule detection (SMD), and single particle tracking (SPT). In this chapter we describe the characteristic properties of a number of lasers commonly used in fluorescence microscopy.
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Keywords
- Continuous Wave
- Second Harmonic Generation
- Optical Parametric Oscillator
- Fluorescence Recovery After Photobleaching
- Fluorescence Correlation Spectroscopy
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Gratton, E., vandeVen, M.J. (2006). Laser Sources for Confocal Microscopy. In: Pawley, J. (eds) Handbook Of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-45524-2_5
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