Abstract
Induction of somatic embryogenesis in roses involves several critical steps requiring specific tissue culture media compositions and particular manipulations of explants. However, it is important to note that although there are various reports on successful induction of somatic embryogenesis in rose, these are often limited to particular genotypes. Therefore, to date, there is no single protocol for inducing somatic embryogenesis that can be used for multiple rose genotypes. Nevertheless, advances have been made in studying regulation of gene expression during somatic embryogenesis. Moreover, successful genetic transformation of rose has been achieved using embryogenic cultures. Transgenic rose lines with desirable traits have now been obtained. Further opportunities for exploiting somatic embryogenesis for genetic manipulation and improvement of roses will become available with all these current achievements and future efforts.
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References
Arene L, Pellegrino C, Gudin S (1993) A comparison of the somaclonal variation level of Rosa hybrida L. cv. Meirutral plants regenerated from callus of direct induction from different vegetative and embryonic tissues. Euphytica 71:83–90
Bird A (2002) DNA methylation patterns and epigenetic memory. Genes Dev 16:6–21
Davies PJ (1995) The plant hormones: their nature, occurrence, and functions. In: Davies PJ (ed) Plant Hormones: Physiology, Biochemistry, and Molecular Biology. Kluwer, Dordrecht, pp 1–12
De Wit JC, Esendam HF, Honkanen JJ, Tuominen U (1990) Somatic embryogenesis and regeneration of flowering plants in rose. Plant Cell Rep 9:456–458
Finnegan EJ (2001) Is plant gene expression regulated globally? Trends Genet 17:361–365
Finnegan EJ, Kovac KA (2000) Plant DNA methyltransferases. Plant Mol Biol 43:189–201
Firoozabady E, Lemieux CS, Moy YS, Moll B, Nicholas JA, Robinson KEP (1991) Genetic engineering of ornamental crops. In vitro 27:96A
Firoozabady E, Moy Y, Courtney-Gutterson N, Robinson K (1994) Regeneration of transgenic rose (Rosa hybrida) plants from embryogenic tissue. Bio/Tech 12:609–613
Hill GP (1967) Morphogenesis of shoot primordia in cultured stem tissue of a garden rose. Nature 216:596–597
Hsia C, Korban SS (1996) Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima. Plant Cell Tiss Org Cult 44:1–6
Kankel MW, Ramsey DE, Stokes TL, Flowers SK, Haag JR, Jeddeloh JA, Riddle NC, Verbsky ML, Richards EJ (2003) Arabidopsis MET1 cytosine methyltransferase mutants. Genetics 163:1109–1122
Kintzois S, Manos C, Makri O (1999) Somatic embrogenesis from mature leaves of rose (Rosa sp.). Plant Cell Rep 18:467–472
Kunitake H, Imamizo H, Mii M (1993) Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thumb). Plant Sci 90:187–194
Levin I, Frankel P, Gilboa N, Tanny S, Lalazar A (2003) The tomato dark green mutation is a novel allele of the tomato homolog of the DEETIOLATED1 gene. Theor Appl Genet 106:454–460
Li, X, Krasnyanski S, Korban SS (2002a) Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa. J Plant Physiol 159:313–319
Li, X, Krasnyanski S, Korban SS (2002b) Optimization of the uidA gene transfer into somatic embryos of rose via Agrobacterium tumefaciens. Plant Physiol Biochem 40:453–459
Li X, Gasic K, Cammue B, Broekaert W, Korban SS (2003) Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew (Sphaerotheca pannosa). Planta 218:226–232
Litz RE, Gray DJ (1995) Somatic embryogenesis for agricultural improvement. World J Microbiol Biotech 11:416–425
Marchant R, Davey MR, Lucas JA, Power JB (1996) Somatic embryogenesis and plant regeneration in floribunda rose (Rosa hybrida L. cvs. Trumpeter and Glad Tidings). Plant Sci 120:95–105
Marchant R, Power JB, Lucas JA, Davey MR (1998a) Biolistic transformation of Rose (Rosa hybrida L.). Ann Bot 81:109–114
Marchant R, Davey MR, Lucas JA, Lamb CJ, Dixon RA, Power JB (1998b) Expression of a chitinase in rose (Rosa hybrida L) reduces development of black spot disease (Diplocarpon rosae Wolf). Mol Breed 4:187–194
Martienssen RA, Colot V (2001) DNA methylation and epigenetic inheritance in plants and filamentous fungi. Science 293:1070–1074
Murli S, Sreedhar D, Lokeswari TS (1996) Regeneration through somatic embryogenesis from petal-derived calli of Rosa hybrida L. Arizona (hybrid tea). Euphytica 91:271–275
Mustilli AC, Fenzi F, Ciliento R, Alfano F, Bowler C (1999) Phenotype of the tomato high pigment-2 mutant is caused by a mutation in the tomato homolog of DEETIOLATED1. Plant Cell 11:145–157
Ng HH, Bird AP (1999) DNA methylation and chromatin modification. Curr Opin Genet Dev 9:158–163
Noriega C, Sondahl MR (1991) Somatic embryogenesis in hybrid tea roses. Bio/Tech 9:991–993
Raemakers CJJM, Jacobsen E, Visser RGF (1995) Secondary somatic embryogenesis and applications in plant breeding. Euphytica 81:93–107
Rout GR, Debata BK, Das P (1991) Somatic embryogenesis in callus culture of Rosa hybrida L. cv. Landora. Plant Cell Tiss Org Cult 27:65–69
Rout GR, Samantaray S, Mottey J, Das P (1999) Biotechnology of the rose: a review of recent progress. Scient Hort 81:201–228
Sarasan V, Roberts AV, Rout GR (2001) Methyl laurate and 6-benzyladenine promote the germination of somatic embryos of a hybrid rose. Plant Cell Rep 20:183–186
Schafer E, Bowler C (2002) Phytochrome-mediated photoperception and signal transduction in higher plants. EMBO Rep 3:1042–1048
Tariq M, Paszkowski J (2004) DNA and histone methylation in plants. Trends Genet 20:244–251
Van der Salm TPM, Bouwer R, van Dijk AJ, Keizer LCP, Hanish Ten Cate CH, Van Der Plas LHW, Dons JJM (1998) Stimulation of scion bud release by rol gene transformed rootstocks of Rosa hybrida L. J Exp Bot 49:847–852
Van der Salm TPM, van der Toorn CJG, Hanischten cate CH, Dons HJM (1996) Somatic embryogenesis and shoot regeneration from excised adventitious roots of the rootstock Rosa hybrida cv. Money Way. Plant Cell Rep 15:522–526
Van der Salm TPM, van der Toorn CJG, Bouwer R, Don HJM (1997) Production of rol gene transformed plants Rosa hybrida L. and characterisation of their rooting ability. Mol Breed 3:39–47
Visessuwan R, Kawai T, Mii M (1997) Plant regeneration systems from leaf segment culture through embryogenic callus formation of Rosa hybrida and R. canina. Breed Sci 47:217–222
Xu ML, Li X, Korban SS (2004) DNA-methylation alterations and exchanges during in vitro cellular differentiation in rose (Rosa hybrida L.). Theor Appl Genet 109:899–910
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Korban, S.S. Somatic Embryogenesis in Rose: Gene Expression and Genetic Transformation. In: Mujib, A., Šamaj, J. (eds) Somatic Embryogenesis. Plant Cell Monographs, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_037
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DOI: https://doi.org/10.1007/7089_037
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