Abstract
Catharanthus roseus is an important medicinal plant that contains two well-known anticancerous alkaloids, vincristine and vinblastine. Cell culture technology has been employed for a long time to improve the alkaloid yield. In this chapter, various processes of somatic embryogenesis such as embryo induction, proliferation, maturation and germination are described. In this embryogenic system, embryos showed irregularities in structure and registered poor conversion frequency. Several carbon sources were added in order to improve the embryo quality before germination: 3% fructose or 3–6% maltose were found to be effective during maturation. Plantlet conversion was high on 3–6% maltose and 3% fructose. In addition, suspension culture, indirect embryogenesis and loss of embryogenic potential with time are discussed in brief. The authors felt that the low yields of vincristine and vinblastine may be improved if the single cell embryo origin concept is utilized in a genetic modification program.
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References
Afreen F, Zobayed SMA, Kozai T (2002) Photoautotropic culture of arabusta somatic embryos: Development of a bioreactor for large scale plantlet conversion from cotyledonary embryos. Ann Bot 90:21–29
Akula A, Becker D, Bateson M (2000) High yielding repetitive somatic embryogenesis and plant recovery in a selected tea clone, “TRI-2025” by temporary immersion. Plant Cell Rep 19:1140–1145
Alemanno L, Berthouly M, Michaux-Ferriere N (1997) A comparison between Theobroma cacao L. zygotic embryogenesis and somatic embryogenesis from floral explants. In Vitro Cell Dev Biol Plant 33:163–172
Bachiri Y, Gazeau C, Hansz J, Morisset C, Dereuddre J (1995) Successful cryopreservation of suspension cells by encapsulation dehydration. Plant Cell Tissue Org Cult 43:241–248
Cho DY, Lee EK, Soh WY (1998) Anomalous structure of somatic embryos developed from leaf explant cultures of Angelica gigasNakai. Korean J Plant Tissue Cult 25:1–5
Corredoira E, Ballester A, Vieitez AM (2003) Proliferation, maturation and germination of Castanea sativa Mill. somatic embryos originated from leaf explants. Ann Bot 92:129–136
Davletova S, Meszaros T, Miskolezi P, Oberschall A, Torok K, Magyar Z, Dudits D, Deak M (2001) Auxins and heat shock activation of a noval member of the calmodulin like domain protein kinase gene family in cultured alfalfa cell. J Exp Bot 52:215–221
Denchev PD, Kullin AI, Scragg AH (1992) Somatic embryo production in bioreactors. J Biotechnol 26:99–109
Dudits D, Bogre L, Gyorgyey J (1991) Molecular and cellular approaches to the analysis of plant embryo development from somatic cells in vitro. J Cell Sci 99:475–484
Etlenne H, Lartaud M, Michaux-Ferriere N, Carron MP, Berthouly M, Teisson C (1997) Improvement of somatic embryogenesis in Hevea brasiliensis (Mull. ARG.) using the temporary immersion technique. In Vitro Cell Dev Biol 33:81–87
Huang L, Chi C, Vits H, Sataba EJ, Cooke TJ, Hu W (1993) Population and biomass kinetics in fed-batch cultures of Daucus carota L. somatic embryos. Biotechnol Bioeng 41:811–818
Hvoslef-Eide AK, Olsen ORS, Lyngved R, Heyerdahl PH (2002) Bioreactor design for clonal propagation of somatic embryo. In: Abstracts of the 1st international symposium on liquid system for in vitro mass propagation of plants, Oslo, Norway, 30 May–1 June
Ilah A, Abdin MZ, Mujib A (2002) Somatic embryo irregularities in vitro cloning of Sandal (Santalum album). Sandalwood Res Newslett 15:2–3
Lee EK, Cho DY, Soh WY (2001) Enhanced production and germination of somatic embryos by temporary starvation in tissue culture of Daucus carota. Plant Cell Rep 20:408–415
Li XY, Feng H, Huang H, Murphy JB, Gbur EE Jr (1998) Polyethylene glycol and maltose enhance somatic embryo maturation in loblolly pine (Pinus teada L.). In vitro Cell Dev Biol Plant 34:22–26
Lipavska H, Konradova H (2004) Somatic embryogenesis in conifers: the role of carbohydrate metabolism. In Vitro Cell Dev Biol Plant 40:23–30
Mannonen L, Toivonen L, Kauppinen VC (1990) Effect of long term preservation on growth and productivity of Panax ginseng and Catharanthus roseus cell culture. Plant Cell Rep 9:173–177
Moreno PRH, Van der Heijden R, Verpoorte R, Vander-Heijden R (1995) Cell and tissue culture of Catharanthus roseus, a literature survey II. Updating from 1988–1993. Plant Cell Tissue Org Cult 42:1–25
Mujib A, Ilah A, Gandotra N, Abdin MZ (2003) In vitro application to improve alkaloid yield in Catharanthus roseus. In: Govil JN, Kumar PA, Singh VK (eds) Biotechnology and genetic engineering. Recent progress in medicinal plants, vol. IV. Sci Tech, Houston, USA, pp 415–440
Owen HR, Wengerd D, Miller AR (1991) Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, active charcoal and medium storage method. Plant Cell Rep 10:583–586
Robichaud RL, Lesser VC, Merkle SA (2004) Treatments affecting maturation and germination of American chestnut somatic embryos. J Plant Physiol 161:957–969
Saito T, Nishizawa S, Nishimura S (1991) Improved culture conditions for somatic embryogenesis from Asparagus officinalis L. using an aseptic ventilative filter. Plant Cell Rep 10:85–89
Sakano K, Kiyota S, Yazaki Y (1997) Acidification and alkalinization of culture medium by Catharanthus roseuscells is anoxic production of lactate a cause of cytoplasmic acidification? Plant Cell Physiol 38:1053–1059
Sharp WR, Sondahl MR, Caldas LS, Maraffa SB (1980) The physiology of in vitro asexual embryogenesis. Hortic Rev 2:268–310
Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues culture in vitro. Symp Soc Exp Biol 11:118–130
Smith DL, Krikorian AD (1990) Somatic embryogenesis of carrot in hormone-free medium: external pH control over morphogenesis. Am J Bot 77:1634–1647
Soh WY, Lee EK, Cho DY (2000) Germination arrest of carrot somatic embryos cultured in MS basal liquid medium. Korean J Plant Tissue Cult 27:175–180
Verma DC, Dougall DK (1977) Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures. Plant Physiol 59:81–85
Wetherell DF, Dougall DK (1976) Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue. Physiol Plant 37:97–103
Xing Z, Powell WA, Maynard CA (1999) Development and germination of American Chesnut somatic embryos. Plant Cell Tissue Org Cult 57:47–55
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Junaid, A., Mujib, A., Bhat, M.A., Ilah, A., Sharma, M.P. Embryogenesis in Catharanthus roseus: Roles of Some External Factors in Proliferation, Maturation and Germination of Embryos. In: Mujib, A., Šamaj, J. (eds) Somatic Embryogenesis. Plant Cell Monographs, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_030
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DOI: https://doi.org/10.1007/7089_030
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