Abstract
Chlorophylls and carotenoids are commonly used as quantitative biomarkers for the composition and biomass of marine phytoplankton. This chapter provides an overview of the molecular diversity of pigment markers, their distribution across algal taxa (based on theories of plastid diversity through endosymbiosis), and their environmental variability. Three new methods for analysis of pigments by HPLC are compared with the original SCOR method. Guidelines for interpreting HPLC pigment chromatograms from field samples are given to determine the likely algal types present, thus enabling optimal computational analysis. Mathematical techniques for analysis of complex pigment data sets (multiple linear regression, inverse simultaneous equations and matrix factorization methods, using CHEMTAX software) are discussed. Methods for converting pigment data to carbon biomass are considered, with suggested strategies for improving biomass estimates.
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Abbreviations
- But-fuco:
-
19′-butanoyloxyfucoxanthin
- Chl:
-
Chlorophyll
- Chl c 2-MGDG:
-
chlorophyll c 2-monogalactosyl diacylglyceride ester. A suffix, e.g. [18:4/ 14:0], denotes the chain lengths (18, 14) and the number of double bonds (4, 0) of the two esterified fatty acids, respectively
- Chlide:
-
chlorophyllide
- DMF:
-
dimethyl formamide
- DV:
-
divinyl
- Hex-fuco:
-
19′-hexanoyloxyfucoxanthin
- HPLC:
-
high performance liquid chromatography
- IS:
-
internal standard
- 4-k Hex-fuco:
-
4-keto-19′-hexanoyloxyfucoxanthin
- MgDVP:
-
magnesium divinyl pheoporphyrin a 5 monomethyl ester
- MV:
-
monovinyl
- Neox:
-
neoxanthin
- Np:
-
non-polar
- SCOR:
-
Scientific Council for Oceanic Research
- TBAA:
-
Tetrabutylammonium acetate
- tr:
-
trace
- Unk:
-
unknown
References
Jeffrey SW, Wright SW (2005) Photosynthetic Pigments in Marine Microalgae. In: Subba Rao DV (ed) Algal Cultures, Analogues of Blooms and Applications. Science Publishers, New Hampshire, USA (in press)
Jeffrey SW, Mantoura RFC, Wright SW (1997) (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris
Jeffrey SW, Wright SW, Zapata M (1999) Mar Freshwater Res 50:879
Garrido JL, Zapata M (2005) Chlorophyll analysis by new high performance liquid chromatography methods. In: Grimm B, Porra RJ, Rüdinger W, Scheer H (eds) Chlorophylls and bacteriochlorophylls: biochemistry, biophysics and biological function. Kluwer Academic, Dordrecht (in press)
Rowan KS (1989) Photosynthetic Pigments of Algae. Cambridge University Press, Cambridge
Young A, Britton G (1993) Carotenoids in Photosynthesis. Chapman & Hall, London
Scheer H (ed) (1991) Chlorophylls. CRC Press, Boca Raton, FL
Scheer H (2003) The pigments. In: Green BR, Parsons WE (eds) Light-harvesting antennas in photosynthesis. Advances in Photosynthesis and Respiration, vol 13. Kluwer Academic Publishers, Dordrecht p 29
Porra RJ, Pfündel EE, Engel N (1997) Metabolism and function of photosynthetic pigments. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 85
Jeffrey SW (1997a) Application of pigment methods to oceanography. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 127
Jeffrey SW, Mantoura RFC, Bjørnland T (1997) Data for the identification of 47 key phytoplankton pigments. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 447
Vernon LP, Seely GR (1966) The Chlorophylls. Academic Press, New York
Zapata M, Jeffrey SW, Wright SW, Rodríguez F, Garrido JL, Clementson L (2004) Mar Ecol Prog Ser 270:83
Strain HH, Cope BT, McDonald GN, Svec WA, Katz JJ (1971) Phytochem 10:1109
Jeffrey SW, Wright SW (1987) Biochim Biophys Acta 894:180–188
Fookes CJR, Jeffrey SW (1989) J Chem Soc Chem Comm 23:1827
Garrido JL, Zapata M (1998) J Phycol 34:70
Jeffrey SW (1989) Chlorophyll c pigments and their distribution in the chromophyte algae. In: Green JC, Leadbeater BSC, Diver WL (eds) The Chromophyte Algae: problems and perspectives. Clarendon Press, Oxford, p 13
Garrido JL, Otero J, Maestro MA, Zapata M (2000) J Phycol 36:497
Bjørnland T (1997) UV-vis spectroscopy of carotenoids. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: guidelines to modern methods, UNESCO monographs on oceanographic methodology, vol 10. UNESCO, Paris p 578
IUPAC Commission on Nomenclature of Organic Chemistry (1975) Pure Appl Chem 41:407
Bjørnland T (1997) Structural relationships between algal carotenoids. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: guidelines to modern methods, UNESCO monographs on oceanographic methodology, vol 10. UNESCO, Paris, p 572
Straub O, Pfander H, Gerspacher M, Rychener M, Schwabe R (1987) Key to Carotenoids. Birkhäuser, Basel
Liaaen-Jensen S (1985) Pure Appl Chem 57:649
Mercandente AZ (1999) Pure Appl Chem 71:2263
Bjørnland T, Liaaen-Jensen S (1989) Distribution patterns of carotenoids in relation to chromophyte phylogeny and systematics. In: Green JC, Leadbeater BSC, Diver WL (eds) The Chromophyte algae: Problems and perspectives. Clarendon Press, Oxford, p 37
Britton G (1995) UV=visible spectroscopy. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids, vol 1B Spectroscopy. Birkhäuser, Basel, p 13
Enzell CR, Back S (1995) Mass spectrometry. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids, vol 1B Spectroscopy. Birkhäuser, Basel, p 261
Liaaen-Jensen S (1995) Combined approach: identification and structure elucidation of carotenoids. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids, vol 1B Spectroscopy. Birkhäuser, Basel, p 343
Johnson PW, Sieburth JM (1979) Limnol Oceanogr 24:928
Waterbury JB, Watson SW, Guillard RRL, Brand LE (1979) Nature 277:293
Li WKW, Wood M (1988) Deep-Sea Res 35:1615
Chisholm SW, Olson RJ, Zettler ER, Goericke R, Waterbury JB, Welshmeyer NA (1988) Nature 334:340
Olson RJ, Chisholm SW, Zettler ER, Armbrust EV (1990) Limnol Oceanogr 35:45
Bodemer U (2004) J Plankton Res 26:1147
Bhattacharya D (1997) Origins of algae and their plastids. Springer, Berlin Heidelberg New York
Delwiche CF (1999) Am Nat 154:S164
McFadden GI (2001) J Phycol 37:951
Palmer JD (2003) J Phycol 39:4
Moreira D, LeGuyader H, Phillipè H (2000) Nature 405:69
Hallegraeff GM, Jeffrey SW (1984) Mar Ecol Prog Ser 20:59
Johnsen G, Samset O, Granskog L, Sakshaug E (1994) Mar Ecol Prog Ser 105:149
Goericke R, Montoya JP (1998) Mar Ecol Prog Ser 169:97
Schlüter L, Møhlenberg F, Havskum H, Larsen S (2000) Mar Ecol Prog Ser 192:49
Henriksen P, Riemann B, Kaas H, Sørensen HM, Sørensen HL (2002) J Plankton Res 24:835
Wood MA (1985) Nature 316:253
Bidigare RR, Schofield O, Prezelin BB (1989) Mar Ecol Prog Ser 56:177
Partensky F, Hoepffner N, Li WKW, Ulloa O, Vaulot D (1993) Plant Physiol 101:285
Sakshaug E, Andresen K (1986) J Plankton Res 8:619
Tukaj Z, Matusiak-Mikulin K, Lewandowska J, Szurkowski J (2003) Plant Physiol Biochem 41:337
Wilhelm SW, Maxwell DP, Trick CG (1996) Limnol Oceanogr 41:89
van Leeuwe MA, Timmermans KR, Witte HJ, Kraay GW, Veldhuis MJW, de Baar HHW (1998) Mar Ecol Prog Ser 166:43
Wilhelm C, Manns L (1991) J Appl Phycol 3:305
Stolte W, Kraay GW, Noordeloos AAM, Riegman R (2000) J Phycol 36:529
Jeffrey SW, Wright SW (1994) Photosynthetic pigments in the Haptophyta. In: Green JC, Leadbeater BSC (eds) The Haptophyte Algae. Clarendon Press, Oxford, p 111
Claustre H, Poulet SA, Williams R, Marty JC, Coombs S, Ben Mlih F, Hapette AM, Martin-Jezéquel V (1990) J Mar Biol Assoc UK 70:197
Vaulot D, Birrien J-L, Marie D, Casotti R, Veldhuis MJW, Kraay GW, Chrétiennot-Dinet M-J (1994) J Phycol 30:1022
Breton E, Brunet C, Sautour B, Brylinski JM (2000) J Plankton Res 22:1423
Mantoura RFC, Jeffrey SW, Llewellyn CA, Claustre H, Morales CE (1997) Comparison between spectrophotometric, fluorometric and HPLC methods for chlorophyll analysis. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 361
Jeffrey SW, Humphrey GF (1975) Biochem Physiol Pflanzen 167:191
Holm-Hansen O, Lorenzen CJ, Holmes RW, Strickland JDH (1965) J Cons Perm Int Explor Mer 30:3
Jacobsen TR (1978) Arch Hydrobiol Beih Ergebn Limnol 16:35
Wright SW, Mantoura RFC (1997) Guidelines for collection and pigment analysis of field samples. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 429
Mantoura RFC, Wright SW, Jeffrey SW, Barlow RG, Cummings DE (1997) Filtration and storage of pigments from microalgae. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 283
Wright SW, Jeffrey SW, Mantoura RFC (1997) Evaluation of methods and solvents for pigment extraction. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 261
Zapata M, Garrido JL (1991) Chromatographia 31:589
Diehn B, Seely GR (1968) Biochim Biophys Acta 153:862
Latasa M, van Lenning K, Garrido JL, Scharek R, Estrada M, Rodríguez F, Zapata M (2001) Chromatographia 53:385
Mantoura RFC, Llewellyn CA (1983) Analytica Chim Acta 151:297
Gieskes WWC, Kraay GW (1983) Mar Biol 75:179
Gieskes WWC, Kraay GW (1986) Mar Biol 92:45
Wright SW, Shearer JD (1984) J Chromatogr 294:281
Roy S (1987) J Chromatogr 391:19
Zapata M, Ayala AM, Franco JM, Garrido JL (1987) Chromatographia 23:26
Garrido JL, Zapata M (1997) Chromatographia 44:43
Van Heukelem L, Thomas C (2001) J Chromatogr 910:31
Wright SW, Jeffrey SW, Mantoura RFC, Llewellyn CA, Welschmeyer N, Bjørnland T, Repeta D (1991) Mar Ecol Prog Ser 78:183
Zapata M, Rodríguez F, Garrido JL (2000) Mar Ecol Prog Ser 195:29
Jeffrey SW, Mantoura RFC (1997) Minimum criteria for identifying phytoplankton pigments. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 631
Airs RL, Atkinson JE, Keely BJ (2001) J Chromatogr A 917:167
Wright SW, Mantoura RFC (1997) Guidelines for selecting and setting up an HPLC system and laboratory. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 383
Jeffrey SW, Wright SW (1997) Qualitative and quantitative HPLC analysis of SCOR reference algal cultures. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 343
Mantoura RFC, Repeta DJ (1997) Calibration methods for HPLC. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris p 407
Jeffrey SW (1974) Mar Biol 26:101
Jeffrey SW, Hallegraeff GM (1987) Mar Ecol Prog Ser 35:649
Barrett J, Jeffrey SW (1964) Plant Physiol 39:44
Barrett J, Jeffrey SW (1971) J Exp Mar Biol Ecol 7:255
Latasa M, Bidigare RR (1998) Deep-Sea Res II 45:2133
Jeffrey SW, Haxo FT (1968) Biol Bull 135:149
Mackey MD, Mackey DJ, Higgins HW, Wright SW (1996) Mar Ecol Prog Ser 144:65
Claustre H, Hooker SB, Van Heukelem L, Berthon JF, Barlowe R, Rasa J, Sessions H, Targa C, Thomas CS, van der Linde D, Marty JC (2004) Mar Chem 85:41
Garibotti IA, Vernet M, Kozlowski WA, Ferrario ME (2003) Mar Ecol Prog Ser 247:27
Prézelin BB, Hofmann EE, Mengelt C, Klinck JM (2000) J Mar Res 58:165
Marty JC, Chiavérini J, Pizay MD, Avril B (2002) Deep-Sea Res II 49:1965
Everitt DA, Wright SW, Volkman JK, Thomas DP, Lindstrom E (1990) Deep Sea Res 37:975
Letelier RM, Bidigare RR, Hebel DV, Ondrusek M, Winn CD, Karl DM (1993) Limnol Oceanogr 38:1420
Bidigare RR, Ondrusek ME (1996) Deep Sea Res II 43:809
Vidussi F, Marty J, Chiavérini J (2000) Deep-Sea Res I 47:423
Wright SW, Thomas DP, Marchant HJ, Higgins HW, Mackey MD, Mackey DJ (1996) Mar Ecol Prog Ser 144:285
Havskum H, Schlüter L, Scharek R, Berdalet E, Jacquet S (2004) Mar Ecol Prog Ser 273:31
Mackey MD, Higgins HW, Mackey DJ, Holdsworth D (1998) Deep-Sea Res 45:1441
Wright SW, van den Enden RL (2000) Deep-Sea Res 47:2363
Carreto JI, Montoya NG, Benavides HR, Guerrero R, Carignan MO (2003) Mar Biol 143:1013
van Lenning K, Latasa M, Estrada M, Sáez AG, Medlin L, Probert I, Véron B, Young B (2003) J Phycol 39:379
Latasa M, Scharek R, Le Gall F, Guilliou L (2004) J Phycol 40:1149
Stoń J, Kosakowska A, Łotocka M, Łysiak-Pastuszak E (2002) Oceanologia 44:419
Andersen RA, Bidigare RR, Keller MD, Latasa M (1996) Deep-Sea Res 43:517
Rodriguez J, Jimenez-Gomez F, Blanco JM, Figueroa FL (2002) Deep Sea Res II 49:693
Rodriguez F, Varela M, Fernández E, Zapata M (2003) Mar Biol 143:995
Ansotegui A, Sarobe A, Trigueros JM, Urrutxurtu I, Orive E (2003) J Plankton Res 25:341
Schlüter L, Møhlenberg F (2003) J Appl Phycol 15:465
Llewellyn CA, Fishwick JR, Blackford JC (2005) J Plankton Res 27:103
Lund JWG, Kipling C, Le Cren ED (1958) Hydrobiol 11:143
Veldhuis MJW, Kraay GW (2004) Deep-Sea Res I 51:507
Staehr PA, Henriksen P, Markager S (2002) Mar Ecol Prog Ser 238:47
Vidussi F, Claustre H, Manca BB, Luchetta A, Marty J-C (2001) J Geophys Res 106:19939
Barlow RG, Aiken J, Holligan PM, Cummings DG, Maritorena S, Hooker S (2002) Deep-Sea Res I 47:637
Gibb SW, Barlow RG, Cummings DG, Rees NW, Trees CC, Holligan P, Suggett D (2000) Prog Oceanogr 45:339
Di Tullio GR, Geesey M, Jones DR, Daly KL, Campbell L, Smith WO (2003) Mar Ecol Prog Ser 255:55
Welschmeyer NA, Lorenzen CJ (1984) Limnol Oceanogr 29:135
Geider RJ (1987) New Phytol 106:1
Geider RJ, La Roche J, Greene R M, Olaizola M (1993) J Phycol 29:755
Taylor AH, Geidler RJ, Gilbert FJH (1997) Mar Ecol Prog Ser 152:51
Riemann B, Simonsen P, Stensgaard L (1989) J Plankton Res 11:1037
Geider RJ, MacIntyre HL, Kana TM (1997) Mar Ecol Prog Ser 148:187
Hillebrand H, Dürselen CD, Kirschtel D, Pollingher D, Zohary T (1999) J Phycol 35:403
Montagnes DJS, Berges JA, Harrison PJ, Taylor FJR (1994) Limnol Oceanogr 39:1044
Menden-Deuer S, Lessard EJ (2000) Limnol Oceanogr 45:569
Redalje DG, Laws EA (1981) Mar Biol 62:73
Gieskes WWC, Kraay GW (1989) Deep Sea Res 36:1127
Goericke R, Welschmeyer NA (1993) Limnol Oceanogr 38:80
Stramski D (1999) Deep-Sea Res 46:335
Schmid H, Bauer F, Stich HB (1998) J Plankton Res 20:1651
Wright SW (1997) Summary of terms and equations used to evaluate HPLC chromatograms. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography: Guidelines to modern methods. UNESCO, Paris, p 622
Egeland ES, Guillard RRL, Liaaen-Jensen S (1997) Phytochem 44:1087
Stauber JL, Jeffrey SW (1988) J Phycol 24:158
Rodríguez F, Pazos Y, Maneiro J, Fraga S, Zapata M (2000) Ninth Int Conf on Algal Blooms, Hobart, Australia
Fawley MW (1991) J Phycol 27:544
Kolber ZS, Plumley FG, Lang AS, Beatty JT, Blankenship RE, VanDover CL, Vetriani C, Koblizek M, Rathgeber C, Falkowski PG (2001) Science 292:2492
Goericke R (2002) Limnol Oceanogr 47:290
Claustre H (1994) Limnol Oceanogr 39:1206
Brunet C, Casotti R, Aronne B, Vantrepotte V (2003) J Plankton Res 25:1413
Demers S, Roy S, Gagnon R, Vignault C (1991) Mar Ecol Prog Ser 76:185
Evens TJ, Kirkpatrick GJ, Millie DF, Chapman DJ, Schofield OME (2001) J Plankton Res 23:1177
Kashino Y, Kudoh S (2003) Phycol Res 51:168
Abrahamsson K, Lorén A, Wulff A, Wängberg S-A (2004) Deep-Sea Res II 51:2789
Andersson M, Van Nieuwerburgh L, Snoeijs P (2003) Mar Ecol Prog Ser 254:213
Bergmann T, Richardson TL, Paerl HW, Pinckney JL, Schofield O (2002) J Plankton Res 24:923
Ediger D, Raine R, Weeks AR, Robinson IS, Sagan S (2001) J Plankton Res 23:893
Furuya K, Hayashi M, Yabushita Y, Ishikawa A (2003) Deep Sea Res 50:367
Gibb SW, Cummings DG, Irigoien X, Barlow RG, Mantoura RFC (2001) Deep Sea Res 48:795
Gin KYH, Zhang S, Lee YK (2003) J Plankton Res 25:1507
Li H-P, Gong G-C, Hsiung T-M (2002) Bot Bull Acad Sin 43:283
Mackey DJ, Blanchot J, Higgins HW, Neveux J (2002) Deep-Sea Res 49:2561
Riegman R, Kraay GW (2001) J Plankton Res 23:191
Steinberg DK, Carlson CA, Bates NR, Johnson RJ, Michaels AF, Knap AH (2001) Deep-Sea Res 48:1405
Suzuki K, Hinuma A, Saito H, Kiyosawa H, Liu H, Saino T, Tsuda A (2005) Prog Oceanogr 64:167
Acknowledgments
Figure 5b was kindly provided by Laurie Van Heukelem. This work was supported by the Australian Government's Cooperative Research Centres Programme through the Antarctic Climate and Ecosystems Cooperative Research Centre (S.W. Wright), and CSIRO Marine Research (S.W. Jeffrey).
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Wright, S.W., Jeffrey, S.W. Pigment Markers for Phytoplankton Production . In: Volkman, J.K. (eds) Marine Organic Matter: Biomarkers, Isotopes and DNA. The Handbook of Environmental Chemistry, vol 2N. Springer, Berlin, Heidelberg. https://doi.org/10.1007/698_2_003
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