Abstract
DNA machines consisting of consecutive hairpins, which we have previously described, have various potential applications in DNA computation. In the present study, a 288-base DNA machine containing four consecutive hairpins was successfully constructed by ligation and PCR. PAGE and fluorescence spectroscopy experiments verified that all four hairpins were successfully opened by four opener oligomers, and that hairpin opening was dependent on the proper openers added in the correct order. Quantitative analysis of the final results by fluorescence spectroscopy indicated that all four hairpins were open in about 1/4 to 1/3 of the DNA machines.
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Keywords
- 10th International Workshop
- Hybridization Chain Reaction
- Stem Sequence
- Page Experiment
- Secondary Structure Design
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Kameda, A., Yamamoto, M., Ohuchi, A., Yaegashi, S., Hagiya, M. (2006). Unravel Four Hairpins!. In: Mao, C., Yokomori, T. (eds) DNA Computing. DNA 2006. Lecture Notes in Computer Science, vol 4287. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11925903_30
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DOI: https://doi.org/10.1007/11925903_30
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-49024-1
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