Abstract
Transients of chlorophyll (Chl) fluorescence emission are widely used to estimate kinetics, yield and regulation of photosynthetic processes in intact plants. In this chapter, we introduce concepts and terms required for a qualified application of the technique. An overview of relevant processes that occur on different timescales, from picoseconds to organism lifetime, is provided as a reference framework for description of approximations and models connecting physiologically relevant photosynthetic parameters and the fluorescence data. Reaching beyond the conventional analysis, we also describe models including Photosystem II heterogeneity and short-living radicals that can affect plant-Chl fluorescence emission. Current state-of-the-art and future prospects for Chl-fluorescence instrumentation are described at the end of the chapter.
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Nedbal, L., Koblížek, M. (2006). Chlorophyll Fluorescence as a Reporter on in vivo Electron Transport and Regulation in Plants. In: Grimm, B., Porra, R.J., Rüdiger, W., Scheer, H. (eds) Chlorophylls and Bacteriochlorophylls. Advances in Photosynthesis and Respiration, vol 25. Springer, Dordrecht. https://doi.org/10.1007/1-4020-4516-6_35
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DOI: https://doi.org/10.1007/1-4020-4516-6_35
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