Summary
The oxygen isotope technique is currently the only reliable method for studying relative electron partitioning between the cytochrome and alternative plant respiratory pathways. The theoretical background to this technique is described, as well as some of the difficulties that can complicate measurements. This chapter describes the development of systems over the last 15 years that currently allow measurement of respiration in both intact tissues and in the aqueous phase. Initially, the focus was on developing on-line systems for both gas and liquid phase measurements, but in recent years attention has shifted to the development of portable off-line systems which will allow measurements of respiratory electron partitioning in field studies. Measurements can now be made much more rapidly and accurately than a decade ago, however, the application of this technique is still limited by the availability of dedicated systems. Finally, a summary of data obtained with this technique is presented.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Angert A and Luz B (2001) Fractionation of oxygen isotopes by root respiration: Implications for the isotopic composition of atmospheric O2. Geochim Cosmochim Acta 65: 1695–1701
Bigeleisen J and Wolfsberg M (1959) Theoretical and experimental aspects of isotope effects in chemical kinetics. Adv Chem Phys 1: 15–76
Day DA, Krab K, Lambers H, Moore AL, Siedow JN, Wagner AM and Wiskich JT (1996) The cyanide-resistant oxidase: To inhibit or not to inhibit, that is the question. Plant Physiol 110: 1–2
Dry IB, Moore AL, Day DA and Wiskich JT (1989) Regulation of alternative pathway activity in plant mitochondria: Nonlinear relationship between electron flux and the redox poise of the quinone pool. Arch Biochem Biophys 273: 148–157
Finnegan PM, Whelan J, Millar AH, Zhang Q, Smith MK, Wiskich JT and Day DA (1997) Differential expression of the multigene family encoding the soybean mitochondrial alternative oxidase. Plant Physiol 114: 455–466
Gaston S, Ribas-Carbo M, Busquets S, Berry JA, Zabalza A and Royuela M (2003) Changes in mitochondrial electron partitioning in response to herbicides inhibiting branched-chain amino acid biosynthesis in soybean. Plant Physiol 133: 1351–1359
Gonzàlez-Meler MA, Ribas-Carbo M, Giles L and Siedow JN (1999) The effect of growth and measurement temperature on the activity of the alternative respiratory pathway. Plant Physiol 120: 765–772
Gonzàlez-Meler M, Thomas RB, Giles L and Siedow JN (2001) Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply. Plant Cell Environ 24: 205–215
Guy RD, Berry JA, Fogel ML and Hoering TC (1989) Differential fractionation of oxygen isotopes by cyanide-resistant and cyanide-sensitive respiration in plants. Planta 177: 483–491
Hayes JM (1983) Practise and principles of isotopic measurements in inorganic geochemistry. In: W.G. Meinschein (ed) Organic Geochemistry of Contemporaneous and Ancient Sediments, pp 5-1–5-31, Great Lakes Section, Society of Economic Paleontologists and Mineralogists, Bloomington, Ind
Henry BK, Atkin OK, Day DA, Millar AH, Menz RI and Farquhar G (1999) Calculation of the oxygen isotope discrimination factor for studying plant respiration. Aust J Plant Physiol 26: 773–780
Hoefnagel MHN, Millar AH, Wiskich JT and Day DA (1995) Cytochrome and alternative respiratory pathways compete for electrons in the presence of pyruvate in soybean mitochondria. Arch Biochem Biophys 318: 394–400
Hoefs J (1987) Stable Isotope Geochemistry. Springer-Verlag, Berlin
Lane GA and Dole M (1956) Fractionation of oxygen isotopes during respiration. Science 123: 574–576
Lennon AM, Neueschwander UH, Ribas-Carbo M, Giles L, Ryals JA and Siedow JN (1997) The effects of salicylic acid and TMV infection upon the alternative oxidase of tobacco. Plant Physiol 115: 783–791
Mariotti A, Germon JC, Hubert P, Kaiser P, Letolle R, Tardieux A and Tardieux P (1981) Experimental determination of nitrogen kinetic isotope fractionation: Some principles; Illustration for the denitrification and nitrification processes. Plant Soil 62: 413–430
Millar AH, Atkin OK, Menz RI, Henry B, Farquhar G and Day DA (1998) Analysis of respiratory chain regulation in roots of soybean seedlings. Plant Physiol 117: 1083–1093
Millenaar FF, Roelofs R, Gonzàlez-Meler MA, Siedow JN Wagner, AM and Lambers H (2000). The alternative oxidase during low-light conditions. Plant J 23: 623–632
Millenaar FF, Gonzàlez-Meler MA, Fiorani F, Welschen R, Ribas-Carbo M, Siedow JN, Wagner AM and Lambers H (2001) Regulation of alternative oxidase activity in six wild monocotyledons species. An in vivo study at the whole root level. Plant Physiol 126:376–387
Moore AL and Siedow JN (1991) The regulation and nature of the cyanide-resistant alternative oxidase of plant mitochondria. Biochim Biophys Acta 1059: 121–140
Nagel OW, Waldron S and Jones HG (2001) An off-line implementation of the stable isotope technique for measurements of alternative respiratory pathway activities. Plant Physiol 127: 1279–1286
Neter J and Wasserman W (1974) Applied Linear Statistical Models: Regression, Analysis of Variance, and Experimental Designs. Richard D. Irwin Inc., Homewood, IL
Noguchi K, Go C-S, Terashima I, Ueda S and Yoshinari T (2001) Activities of the cyanide-resistant respiratory pathway in leaves of sun and shade species. Aust J Plant Physiol 28: 27–35
Peñuelas J, Ribas-Carbo M and Giles L (1996) Effects of allelochemicals on plant respiration and oxygen isotope fractionation by the alternative oxidase. J Chem Ecol 22: 801–805
Ribas-Carbo M, Berry JA, Yakir D, Giles L, Robinson SA, Lennon AM and Siedow JN (1995) Electron partitioning between the cytochrome and alternative pathways in plant mitochondria. Plant Physiol 109: 829–837
Ribas-Carbo M, Lennon AM, Robinson SA, Giles L, Berry J and Siedow JN (1997) The regulation of the electron partitioning between the cytochrome and alternative pathways in soybean cotyledon and root mitochondria. Plant Physiol 113: 903–911
Ribas-Carbo M, Aroca R, Gonzàlez-Meler MA, Irigoyen JJ and Sanchez-Diaz M (2000a) The electron partitioning between the cytochrome and alternative respiratory pathways during chilling recovery in two cultivars differing in chilling sensitivity. Plant Physiol 122:199–204
Ribas-Carbo M, Robinson SA, Meler-Gonzàlez MA, Lennon AM, Giles L, Siedow JN and Berry JA (2000b) Effects of light on respiration and oxygen isotope fractionation in soybean cotyledons. Plant Cell Environ 23: 983–989
Robinson SA, Yakir D, Ribas-Carbo M, Giles L, Osmond CB, Siedow JN and Berry JA (1992) Measurements of the engangement of cyanide-resistant respiration in the Crassulacean acid metabolism plant Kalanchoë daigremontiana with the use of on-line oxygen isotope discrimination. Plant Physiol 100: 1087–1091
Robinson SA, Ribas-Carbo M, Yakir D, Giles L, Reuveni Y and Berry JA (1995) Beyond SHAM and cyanide: Opportunities for studying the alternative oxidase in plant respiration using oxygen isotope discrimination. Aust J Plant Physiol 22: 487–496
Saisho D, Nambara, E, Naito s, Tsutsumi N, Hirai A and Nakazono M. (1997). Characterization of the gene family for alternative oxidase from Arabidopsis thaliana. Plant Mol Biol 35: 585–596
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer
About this chapter
Cite this chapter
Ribas-Carbo, M., Robinson, S.A., Giles, L. (2005). The Application of the Oxygen-Isotope Technique to Assess Respiratory Pathway Partitioning. In: Lambers, H., Ribas-Carbo, M. (eds) Plant Respiration. Advances in Photosynthesis and Respiration, vol 18. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3589-6_3
Download citation
DOI: https://doi.org/10.1007/1-4020-3589-6_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3588-3
Online ISBN: 978-1-4020-3589-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)