Abstract
The use of in situ hybridization (ISH) for the detection of mRNAs in cell bodies has greatly expanded our ability to detect cellular phenotypes in the central nervous system. Riboprobes have been used in the past to identify neuropeptide precursors, distribution of receptors, ion channels, and enzymes. More recently, the discovery of unambiguous markers for the major ionotropic transmitters has made possible the definitive identification of neurons involved in fast transmission. The advantages and disadvantages of different types of probes, including DNA probes, oligonucleotides, and RNA probes for the detection of mRNAs are described. Although in situ hybridization was pioneered with the use of radioactive probes, nonradioactive alternatives are now readily available. The relative merits of nonradioactive probes, specifically for combination with tract-tracing, are discussed. This chapter focuses on in situ hybridization methods based on nonradioactive riboprobes and their use in combination with tract-tracing and immunocytochemistry.
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Stornetta, R.L., Guyenet, P.G. (2006). Nonradioactive In Situ Hybridization in Combination with Tract-Tracing. In: Zaborszky, L., Wouterlood, F.G., Lanciego, J.L. (eds) Neuroanatomical Tract-Tracing 3. Springer, Boston, MA . https://doi.org/10.1007/0-387-28942-9_8
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DOI: https://doi.org/10.1007/0-387-28942-9_8
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