Abstract
Information concerning the immobilization and hybridization of DNA on a surface is paramount to the development of DNA-based electronic biosensors. This study looks at recent investigations of DNA immobilized on gold surfaces using standard electrochemical techniques such as cyclic voltammetry (CV), potential step chronocoulometry and electrochemical impedance electrochemical impedance spectroscopy (EIS). The thiol-gold linkage is exploited for the immobilization of single- and double-stranded DNA onto gold electrodes. Two redox markers of opposite charge, ferricyanide and ruthenium hexaammine, respectively, are used to probe the environment in the vicinity of thiol-derivatized DNA electrodes. M-DNA is a form of DNA which allows the specific incorporation of certain metal ions into its helical structure under stringent conditions (i.e. low ionic strength and pH of 8.5). Single-stranded DNA monolayer and double strands DNA monolayer resistances were evaluated using EIS, respectively, and CV response were compared each other. The addition of Zn2+, under M-DNA formation conditions, led to a dramatic enhancement of electrochemical response compared to B-DNA.
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Li, CZ., Long, YT., Sutherland, T., Lee, J.S., Kraatz, HB. (2006). Electronic Biosensors Based on DNA Self-Assembled Monolayer on Gold Electrodes. In: Xing, WL., Cheng, J. (eds) Frontiers in Biochip Technology. Springer, Boston, MA. https://doi.org/10.1007/0-387-25585-0_17
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DOI: https://doi.org/10.1007/0-387-25585-0_17
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