Overview
- Designed for both reference and teaching with clear mathematical and physical formulations of far-field optical microscopy techniques
- Contains English translations of seminal papers that are historically and pedagogically relevant
- Gives detailed tutorials on many optical topics that are underdeveloped in similar books
- Discusses the ethical dimensions of the discovery of each relevant microscope
- Emphasizes the importance of attribution of credit for inventions and appropriate citation of prior works
- Presents a critical analysis of the benefits and limitations of each microscopic technique
Part of the book series: Springer Series in Optical Sciences (SSOS, volume 227)
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About this book
The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localizationmicroscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant.
This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.
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Keywords
Table of contents (16 chapters)
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Development of Microscopes
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Optical Techniques to Enhance Contrast in the Microscope
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Far-Field Superresolution Optical Microscopy
Reviews
“The book is a must for anyone working in optics as well as for anyone interested in science in general. It is very clearly written, touches on many historical aspects of optics and has many photos supporting the text information.” (Daniela Dragoman, Optics & Photonics News, osa-opn.org, July 16, 2020)
Authors and Affiliations
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Bibliographic Information
Book Title: Superresolution Optical Microscopy
Book Subtitle: The Quest for Enhanced Resolution and Contrast
Authors: Barry R. Masters
Series Title: Springer Series in Optical Sciences
DOI: https://doi.org/10.1007/978-3-030-21691-7
Publisher: Springer Cham
eBook Packages: Physics and Astronomy, Physics and Astronomy (R0)
Copyright Information: Springer Nature Switzerland AG 2020
Hardcover ISBN: 978-3-030-21690-0Published: 23 March 2020
Softcover ISBN: 978-3-030-21693-1Published: 26 August 2021
eBook ISBN: 978-3-030-21691-7Published: 21 March 2020
Series ISSN: 0342-4111
Series E-ISSN: 1556-1534
Edition Number: 1
Number of Pages: XXII, 403
Number of Illustrations: 19 b/w illustrations, 31 illustrations in colour
Topics: Spectroscopy and Microscopy, Biological Microscopy, Microwaves, RF and Optical Engineering, Optics, Lasers, Photonics, Optical Devices, History of Science