Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1090)
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About this book
In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Plant Metabolic Flux Analysis, seeks to aid scientists in the further study of cutting-edge protocols and methodologies that are crucial to getting ahead in MFA.
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Keywords
Table of contents (21 protocols)
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Experimental Design and Acquistion of Labeling Data (Experimental Design and Data Acquisition for Isotopic Labeling Based Metabolic Flux Analysis)
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From Labeling Data to Flux Maps (from the Labeling Data to a Metabolic Map)
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Predicting Fluxes in Plants
Editors and Affiliations
Bibliographic Information
Book Title: Plant Metabolic Flux Analysis
Book Subtitle: Methods and Protocols
Editors: Martine Dieuaide-Noubhani, Ana Paula Alonso
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-688-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2014
Hardcover ISBN: 978-1-62703-687-0Published: 13 November 2013
Softcover ISBN: 978-1-4939-6050-7Published: 20 September 2016
eBook ISBN: 978-1-62703-688-7Published: 13 November 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 365
Number of Illustrations: 44 b/w illustrations, 31 illustrations in colour
Topics: Plant Sciences, Plant Biochemistry, Metabolomics