Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1039)
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About this book
In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
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Keywords
- DNA
- DNA for amplification and analysis
- RNA
- RNA monitoring in live cells
- capillary electrophoresis
- fluorescent in situ hybridization
- isothermal amplification techniques
- microarrays
- molecular beacon probes for micro RNA analysis
- nucleic acid detection methods
- point-of care diagnostics
- qPCR chemistries
- signal amplification technique
- visual or electric signals
Table of contents (24 protocols)
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New Real-Time and Instantaneous Assays
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Isothermal Amplification Methods for Nucleic Acid Detection
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Signal Amplification Approach
Editors and Affiliations
Bibliographic Information
Book Title: Nucleic Acid Detection
Book Subtitle: Methods and Protocols
Editors: Dmitry M. Kolpashchikov, Yulia V. Gerasimova
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-535-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2013
Hardcover ISBN: 978-1-62703-534-7Published: 13 September 2013
Softcover ISBN: 978-1-4939-6041-5Published: 23 August 2016
eBook ISBN: 978-1-62703-535-4Published: 12 September 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 320
Number of Illustrations: 27 b/w illustrations, 58 illustrations in colour
Topics: Human Genetics, Nucleic Acid Chemistry