Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 807)
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About this book
Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.
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Keywords
Table of contents (18 protocols)
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Front Matter
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Back Matter
Editors and Affiliations
Bibliographic Information
Book Title: Adeno-Associated Virus
Book Subtitle: Methods and Protocols
Editors: Richard O. Snyder, Philippe Moullier
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-370-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-369-1Published: 28 October 2011
Softcover ISBN: 978-1-4939-6175-7Published: 23 August 2016
eBook ISBN: 978-1-61779-370-7Published: 29 October 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 462
Number of Illustrations: 23 b/w illustrations, 20 illustrations in colour
Topics: Animal Genetics and Genomics, Human Genetics, Gene Therapy