Overview
- The methods chapters are preceded by several excellent reviews that highlight the role of post-translational modifications and subcellular trafficking in transcription factor function
- Includes new techniques for assessing kinase targeting of specific transcription factors, sites of phosphorylation and the involved signaling pathways
- Describes novel molecular and elegant microscopic imaging-based procedures to evaluate nuclear translocation and export in living cells
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 647)
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About this book
In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today.
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Keywords
Table of contents (24 protocols)
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Transcription Factor Trafficking and Post-translational Modifications: Overviews of Mechanisms
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Cytoplasmic-Nuclear Trafficking of Transcription Factors
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Post-translational Modifications and Impact on Function
Editors and Affiliations
Bibliographic Information
Book Title: Transcription Factors
Book Subtitle: Methods and Protocols
Editors: Paul J. Higgins
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-738-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60761-737-2Published: 28 June 2010
Softcover ISBN: 978-1-4939-5715-6Published: 23 August 2016
eBook ISBN: 978-1-60761-738-9Published: 09 August 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 426
Topics: Cell Biology